• Journal of Environmental Health Sciences
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• v.40 no.3
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• pp.234-244
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• 2014

Objectives: This study was carried out in order to analyze the composition of the leaves and stems of Abeliophyllum distichum Nakai, with the aim of obtaining basic data for utilizing the plant as a food ingredient, as well as for processing. Methods: Leaves and stems from Abeliophyllum distichum Nakai were harvested at Cheongcheon-myeon, Geosan-gun, Chungcheongbuk-do, and were subsequently freeze-dried and ground to a fine powder for chemical component analysis and safety evaluation. Results: The moisture contents of Abeliophyllum distichum Nakai leaves and stems were respectively 65.07% and 40.97%, and the crude ash contents were 1.32% and 0.91%. In addition, the crude protein contents were 11.97% and 3.77%, and the crude fat contents were 2.52% and 0.36%, respectively. The fructose and glucose contents were 32.13 mg/g and 56.17 mg/g for leaves, and 11.38 mg/g and 10.59 mg/g for stems. The major fatty acids of the leaves were palmitic acid (31.79%) and stearic acid (14.79%), and those for stems were linolenic acid (32.78%) and palmitic acid (26.75%). The ascorbic acid contents of leaves and stems were 1.32 mg/g and 0.30 mg/g respectively. The calcium content was found to be the highest among the minerals tested, both in the leaves and stems, with the levels being 166.17 mg/100 g for leaves and 592.34 mg/100 g for stems. The content of organic acid was greater in leaves than in stems, with that of malic acid accounting for more than 75% of total organic acids for both samples. The total phenolic compounds and flavonoid contents of Abeliophyllum distichum Nakai were 50.64 mg/g and 13.53 mg/g in leaves and 96.47 mg/g and 18.53 mg/g in stems, respectively. No changes were shown in the number of micronucleated polychromatic erythrocytes (MNPCE) among 2,000 polychromatic erythrocytes compared to the negative control. Abeliophyllum distichum Nakai was administered orally to rats in order to investigate acute toxicity. The $LD_{50}$ values in rats were above 2,000 mg/kg. Conclusion: These results indicate that the leaves and stems of Abeliophyllum distichum Nakai can be used as natural ingredients in the development of nutritional and functional materials.

• The Korean Journal of Food And Nutrition
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• v.36 no.1
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• pp.42-49
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• 2023

To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.

• Food Science and Preservation
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• v.25 no.1
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• pp.27-35
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• 2018

In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.

• Nutrition Research and Practice
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• v.16 no.4
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• pp.419-434
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• 2022

BACKGROUND/OBJECTIVES: Benign prostatic hyperplasia (BPH) is the most common prostate disease and one of the most common chronic diseases caused by aging in men. On the other hand, there has been no research on BPH using Abeliophyllum distichum Nakai (A. distichum). Therefore, this study investigated the effects of A. distichum on BPH. MATERIALS/METHODS: A. distichum leaves were extracted with distilled water, 70% ethanol, and 95% hexane as solvents. Subsequently, the inhibitory effects of each A. distichum extract on androgen receptor (AR) signaling were evaluated in vitro. The testosterone-induced BPH model was then used to confirm the efficacy of A. distichum leaves in 70% ethanol extract (ADLE). RESULTS: ADLE had the strongest inhibitory effect on AR signaling. A comparison of the activity of ADLE by harvest time showed that the leaves of A. distichum harvested in autumn had a superior inhibitory effect on AR signaling to those harvested at other times. In the BPH rat model, the administration of ADLE reduced the prostate size and prostate epithelial cell thickness significantly and inhibited AR signaling. Subsequently, the administration of ADLE also reduced the expression of growth factors, thereby inactivating the PI3K/AKT pathway. CONCLUSIONS: An analysis of the efficacy of ADLE to relieve BPH showed that the ethanol extract grown in autumn exhibited the highest inhibitory ability of the androgen-signaling related factors in vitro. ADLE also inhibited the expression of growth factors by inhibiting the expression of the androgen-signaling related factors in vivo. Overall, ADLE is proposed as a functional food that is effective in preventing BPH.

• Korean Journal of Plant Resources
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• v.31 no.6
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• pp.667-674
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• 2018

In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at $121^{\circ}C$, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. $IC_{50}$ values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.

• Korean Journal of Environment and Ecology
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• v.18 no.2
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• pp.210-220
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• 2004

The purpose of this study is to propose the raw data of conservation and restoration by analyzing the environmental and growth characteristics in Abeliophyllum distichum habitats. The types of soil were sandy loam, loam and silty clay loam and the correlation on chemical characteristics of soil appeared that T-N and C.E.C were highly correlative. In case of growth characteristics, height and diameter in 1st site, crown in 2nd site, number of leaves in 5th site and number of seeds in 4th site were better than other sites. In the results of correlation on growth characteristics, height and diameter were highly correlative. In case of growth and chemical characteristics, EC and height were highly correlative. In the results of regression analysis, R-square of height and EC was some 70.4％ and that of number of leaves and T-N was some 70.2%. To conserve and restore Abeliophyllum distichum habitats, there were demanded that the accurate environmental analysis that considered such diverse factors as light intensity, temperature, genetic quality and so forth.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.231-236
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• 2015

Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at $100^{\circ}C$, 70% ethanol at $85^{\circ}C$, and 70% ethanol with ultrasonication at $60^{\circ}C$ The ultrasonicated extract showed 95.62% of the highest cell viability in addition of $0.3mg/m{\ell}$ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to $2908.1pg/m{\ell}$ with ultrasonicated extract while $6640.8pg/m{\ell}$ with water extract and $3609.3pg/m{\ell}$ with ethanol extract, in addition of $0.3mg/m{\ell}$. Collagen production was increased up to $154.7ng/m{\ell}$ in addition of ultrasonicated extract, and followed by $121.4ng/m{\ell}$ and $31.2ng/m{\ell}$ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.4
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• pp.149-160
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• 1995

We investigated the seasonal changes flower color of 163 deciduous woody landscape plants in the Suwon region from January 1, 1992 to March 20, 1993. The results were as follows; 1. By the month of anthesis of woody landscape plants, only one plant of Hamamelis japonica flowered in February, 15 species in March, 48 species in April, 63 species in May, 23 species in June, 12 species in July, and one plant of Hydrangea paniculata was flowered in August. 2. The flowering period was about 220 days from February 24, 1992 that Hamamelis japonica was anthesis to October 5, 1992 when Hydrangea paniculata was deblossomed. 3. By the flowering period of woody landscape plants, 81 species continued for 11 days through 20 days, and Rosa spp., 118 days, Hibiscus syriacus 'Yungkwang', 80 days, Largerstroemia indica, 65 days, and 6 species continued for 41 through 60 days, 10 species were 31 through 40 days, 43 species were 21 through 30 days, and 20 species were for less than 11 days. 4. The woody landscape plants flowering before leaf spreading, Hamamelis japonica, Abeliophyllum distichum, Prunus mume 'Hwahyangmi', Prunus mume 'Baekkaha', Lindera obtusiloba, Cornus officinalis, Prunus armeniaca. The others were plants with leaves spreading white flowering; Forsythia ovata 'Tetra gold', Forsythia ovata, Corylus hetrophylla, Rhododendron mucronulatum, Magnolia denudata, Forsythia koreana 'Seoul Gold', Forsythia koreana, Magnolia stellata, Acer negundo 'Elegans', Magnolia kobus, Forsythia viridissima 'Bronxensis', Prunus yedoensis, Prunus leveilleana var. pendula, Prunus persica for. albiplena, Prunus tomentosa, Prunus persia, Magnolia liliflora, Prunus glandulosa for. sinensis, Cercis chinensis, Poncirus trifoliata. 5. In terms of flower color based on KBS standard color number, 83 species were white, 44 species wer red, 21 species were yellow, 12 species were violet, and 3 species were green. 6. In terms of the flower color by month. Hamamelis japonica was yellow February. Flower colors in March were : yellow-7 species, red-3 species and white-5 species. Flower colors in April were : White-21 species, red-19 species and yellow-6 species. Flower colors in May were : White-36 species, red-16 species. The white flowers in June were 16 species. Flower colors in July were : white-4 species, red-4 species. 7. The white flower color of woody landscape plants of trees was 35 species. The red flower color was 18 species, yellow flower color was 5 species, violet flower color was 2 species, and green flower color was 3 species. Also the white flower color of woody landscape plants of shrubs was 48 species, red flower color was 25 species, yellow flower color was 17 species and violet flower color was 10 species. 8. The new 'Cultivars' of woody landscape plants are needed to introduced the development of planting design. 9. Present data of illustrated books of plants should be checked by new data that was studied in this research.