Browse > Article
http://dx.doi.org/10.7732/kjpr.2018.31.6.667

Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts  

Jang, Tae-Won (Department of Medicinal Plant Resources, Andong National University)
Choi, Ji-Soo (Department of Medicinal Plant Science, Jungwon University)
Kim, Hoi-Ki (Fanipinkorea Co., Ltd)
Lee, Eun-Ja (Fanipinkorea Co., Ltd)
Han, Man-Wook (Fanipinkorea Co., Ltd)
Lee, Ki-Beom (Incheon Business Information Technopark. Biotechnology & Business Center)
Kim, Do-Wan (Department of Food Science and Industry, Jungwon University)
Park, Jae-Ho (Department of Pharmaceutical Science, Jungwon University)
Publication Information
Korean Journal of Plant Resources / v.31, no.6, 2018 , pp. 667-674 More about this Journal
Abstract
In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at $121^{\circ}C$, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. $IC_{50}$ values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.
Keywords
Abeliophyllum distichum; MITF; TRP-1; TRP-2; Tyrosinase;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Seo, J.Y., K.H. Cho, H.C. Eun and J.H. Chung. 2001. Skin aging from phenotype to mechanism. Korean J. Investig. Dermatol. 8(1):187-194.
2 Son, Y.O., S.A. Lee, S.S. Kim, Y.S. Jang, J.C. Chun and J.C. Lee. 2011. Acteoside inhibits melanogenesis in B16F10 cells through ERK activation and tyrosinase down-regulation. J. Pharm. Pharmacol. 63(10):1309-1319.   DOI
3 Tsatmali, M., J. Ancans and A.J. Thody. 2002. Melanocyte function and its control by melanocortin peptides. J. Histochem. Cytochem. 50(2):125-133.   DOI
4 Unver, N., P. Freyschmidt-Paul, S. Horster, H. Wenck, F. Stab, T. Blatt and H.P. Elsasser. 2006. Alterations in the epidermal melanin axis and factor XIIIa melanophages in senile lentigo and ageing skin. Br. J. Dermatol. 155(1):119-128.   DOI
5 Van den Berg, R., G.R. Haenen, H. Van den Berg and A. Bast. 1999. Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem. 66(4):511-517.   DOI
6 Wickens, A.P. 2001. Ageing and the free radical theory. Respir. Physiol. 128(3):379-391.   DOI
7 Yang, E.S., J.S. Hwang, H.C. Choi, R.H. Hong and S.M. Kang. 2008. The effect of genistein on melanin synthesis and in vivo whitening. Korean J. Soc. Microbiol. Biotechnol. 36(1):72-81.
8 Ahn, J.H., S.H. Jin and H.Y. Kang. 2008. LPS induces melanogenesis through p38 MAPK activation in human melanocytes. Arch. Dermatol. Res. 300(6):325-329.   DOI
9 Ahn, S.J., M. Koketsu, H. Ishihara, S.M. Lee, S.K. Ha, K.H. Lee, T.H. Kang and S.Y. Kim. 2006. Regulation of melanin synthesis by selenium-containing carbohydrates. Chem. Pharm. Bull. 54(3):281-286.   DOI
10 Bentley, N.J., T. Eisen, and C.R. Goding. 1994. Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator. Mol. Cell. Biol. 14(12):7996-8006.   DOI
11 Bondet, V., W. Brand-Williams and C. Berset. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT Food Sci. Technol. 30(6):609-615.   DOI
12 Bremer, B., K. Bremer, N. Heidari, P. Erixon, R.G. Olmstead, A.A. Anderberg, M. Kallersjo and E. Barkhordarian. 2002. Phylogenetics of asteroids based on 3 coding and 3 non-coding chloroplast DNA markers and the utility of non-coding DNA at higher taxonomic levels. Mol. Phylogenet. Evol. 24(2):274-301.   DOI
13 Brenner, M. and V.J. Hearing. 2008. The protective role of melanin against UV damage in human skin. Photochem. Photobiol. 84(3):539-549.   DOI
14 Chung, J.H., V.N. Hanft and S. Kang. 2003. Aging and photoaging. J. Am. Acad. Dermatol. 49(4):690-697.   DOI
15 Costin, G.E. and V.J. Hearing. 2007. Human skin pigmentation: melanocytes modulate skin color in response to stress. FASEB J. 21(4):976-994.   DOI
16 Curto, E.V., C. Kwong, H. Hermersdorfer, H. Glatt, C. Santis, V. Virador, V.J. Hearing and T.P. Dooley. 1999. Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem. Pharmacol. 57(6):663-672.   DOI
17 Eberlein-Konig, B., M. Placzek and B. Przybilla. 1998. Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and d-${\alpha}$-tocopherol (vitamin E). J Am Dermatol 38(1):45-48.   DOI
18 Ferreira, I.C., P. Baptista, M. Vilas-Boas and L. Barros. 2007. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 100(4):1511-1516.   DOI
19 Fisher, G.J., S. Kang, J. Varani, Z. Bata-Csorgo, Y. Wan, S. Datta and J.J. Voorhees. 2002. Mechanisms of photoaging and chronological skin aging. Arch. Dermatol. 138(11):1462-1470.
20 He, Z.D., K.M. Lau, H.X. Xu, P.C. Li and B.P. Pui-Hay. 2000. Antioxidant activity of phenylethanoid glycosides from Brandisia hancei. J. Ethnopharmacol. 71(3):483-486.   DOI
21 Jang, T.W. and J.H. Park. 2018. Antioxidant activity and inhibitory effects on oxidative DNA damage of callus from Abeliophyllum distichum Nakai. Korean J. Plant. Res. 31(3):228-236.   DOI
22 Hearing, V.J. and M. Jimenez. 1987. Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19(12):1141-1147.   DOI
23 Hearing, V.J. and K. Tsukamoto. 1991. Enzymatic control of pigmentation in mammals. FASEB J. 5(14):2902-2909.   DOI
24 Iozumi, K., G.E. Hoganson, R. Pennella, M.A. Everett and B.B. Fuller. 1993. Role of tyrosinase as the determinant of pigmentation in cultured human melanocytes. J. Invest. Dermatol. 100(6):806-811.   DOI
25 Jimenez-Cervantes, C., F. Solano, T. Kobayashi, K. Urabe, V.J. Hearing, J.A. Lozano and J.C. Garcia-Borron. 1994. A new enzymatic function in the melanogenic pathway. The 5,6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP-1). J. Biol. Chem. 269(27):17993-18000.
26 Kim, B., Y.E. Choi and H.S. Kim. 2014. Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator-activated receptor (PPAR)-${\alpha}$ and suppression of inflammatory cytokines. Phytother. Res. 28(9):1359-1366.   DOI
27 Kim, Y.J. and H. Uyama. 2005. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell. Mol. Life Sci. 62(15):1707-1723.   DOI
28 Li, G., Ju, H.K. and H.W. Chang, Y. Jahng, S.H. Lee and J.K. Son. 2003. Melanin biosynthesis inhibitors from the bark of Machilus thunbergii. Biol. Pharm. Bull. 26(7):1039-1041.   DOI
29 Lee, S.Y., E.J. Hwang, G.H. Kim, Y.B. Choi, C.Y. Lim and S.M. Kim. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Korean J. Med. Crop Sci. 13(3):93-100.
30 Li, X., Y. Xie, K. Li, A. Wu, H. Xie, Q. Guo, P. Xue, Y. Maleshibek, W. Zhao, J. Guo, and D. Chen. 2018. Antioxidation and cytoprotection of acteoside and its derivatives: Comparison and mechanistic chemistry. Molecules 23(2):498.   DOI
31 Luft, R. 1994. The development of mitochondrial medicine. PNASU. 91(19):8731-8738.   DOI
32 Marnett, L. 2000. Oxiradicals and DNA damage. Carcinogenesis 21(3):361-370.   DOI
33 Masaki, H., S. Sakaki, T. Atsumi, and H. Sakurai. 1995. Active oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18(1):162-166.   DOI
34 Maxwell, S.R. 1995. Prospects for the use of antioxidant therapies. Drugs 49(3):345-361.   DOI
35 Oh, H.C., D.G. Kang, T.O. Kwon, K.K. Jang, K.Y. Chai, Y.G. Yun, H.T. Chung and H.S. Lee. 2003. Four glycosides from the leaves of Abeliophyllum distichum with inhibitory effects on angiotensin converting enzyme. Phytother. Res. 17(7):811-813.   DOI
36 Oyaizu, M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J. Nutr. 44(6): 307-315.   DOI
37 Park, H.Y., M. Kosmadaki, M. Yaar and B.A. Gilchrest. 2009. Cellular mechanisms regulating human melanogenesis. Cell. Mol. Life Sci. 66(9):1493-1506.   DOI
38 Park, J.H. 2011. Antioxidant activities and inhibitory effect on oxidative DNA damage of extracts from Abeliophylli distichi Folium. Korean J. Herbol. 26(4):95-99.
39 Que, F., L. Mao and X. Pan. 2006. Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Res. Int. 39(5):581-587.   DOI
40 Schlesier, K., M. Harwat, V. Bohm and R. Bitsch. 2002. Assessment of antioxidant activity by using different in vitro methods. Free Radic. Res. 36(2):177-187.   DOI