• Korean Journal of Veterinary Service
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• v.32 no.1
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• pp.83-85
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• 2009

In a cross-sectional abattoir survey of bovine pulmonary diseases in Mymensingh, during September 2001 to April 2002, subclinical Dictyocaulus viviparous infection was found in mesoendemic level in Bangladeshi adult zebu cattle. The number of mature lung worms varied from $1{\sim}18$ in individual lungs. One to five mature lung worms were most frequent (74.2%) followed by 6 to 10(19.3%) and $11{\sim}18$ (6.5%). Of the total 123 mature worms collected from 31 lungs, there were 50 (40.7%) male and 73 (59.3%) female worms. The female lungworms (mean length 14.41mm) were significantly (P<0.01) larger than the male lungworms (mean length 11.28mm). The number of hydatid cysts varied from $1{\sim}80$ in individual lungs. One to five ($1{\sim}5$) cysts were recorded most commonly (76.0%) followed by $11{\sim}80$ (20.0%) and $6{\sim}10$ (4%). The size (diameter) of the hydatid cysts ranged from $2{\sim}12cm$. Of the total 203 hydatid cysts collected from 31 lungs 45 (22.2%) cysts were fertile and 158 (77.8%) cyst were sterile.

• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.154-158
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• 1987

As a series of systematic classification of paramphistomes, the worms in the rumen and reticulum were collected on 214 Korean cattle slaughtered at Jeonju abattoir from January, 1986 to April, 1987 and were classified by means of morphology. Afterwards, the karyotype of Paramphistomum cervi(Zeder, 1790) was detected by means of modified air.drying method from germ cells of the worms. The results were summarized as follows: 1. In the chromosome number of 254 p. cervi, the haploid cell was n:9 and the diploid 2n=18. The meiotic divisions were observed frequently; 1,924 haploid and 32 diploid cells were reliable. Nine pairs of mitotic chromosomes were homologous in the metaphase stage, and the chromosomes were composed of five medium-sized metacentrics (m) , subtelocentrics(st) or submetacentrics(sm) and four small-siRed subtelocentrics(st) or submetacentrics(sm). Meiotic metaphase was composed of five medium and four small chromosomes in size. 2. As a series of C-banding method, C-band was showed in centromeric region from all of the haploid germ cells. Whereas chromosome No. 3 and 5 included heterochromatin on the tip region, chromosome No. 4 on the distal region and No. 6 proximal region. And chromosomes No. 2 and 8 showed a remarkable C-band distinguished from other chromosomes.

• Food Science of Animal Resources
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• v.41 no.1
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• pp.135-144
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• 2021

This study determined the antibiotic resistance patterns of Escherichia coli and Staphylococcus aureus from the raw meat and feces of three game species from three different farms across South Africa. The Kirby-Bauer disk diffusion method was used according to the Clinical and Laboratory Standards Institute 2018 guidelines. E. coli was tested against ampicillin, ceftazidime, chloramphenicol, streptomycin, sulphafurazole and tetracycline. S. aureus was tested against tetracycline, erthromycin, vancomycin, penicillin, oxacillin and cefoxitin. There were no significant differences in the E. coli antibiotic resistance profiles between the meat and fecal samples (except towards ceftazidime where 5% of the meat isolates were resistant and 0% of the fecal isolates). The S. aureus meat isolates showed high (75%) resistance towards penicillin and on average, 13% were resistant to oxacillin/ cefoxitin, indicating methicillin resistance. The results from this study indicate that there is incidence of antibiotic resistant bacteria from the feces and meat of wildlife species across South Africa, suggesting that cross contamination of the meat occurred during slaughter by antibiotic resistant bacteria from the abattoir personnel or equipment and or from carcass fecal matter. In addition, the results highlight the importance of food safety and hygiene procedures during slaughter to prevent cross-contamination of antibiotic resistant bacteria, as well as pathogens, onto raw meat.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1049-1054
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• 2003

A group of Hanwoo (Korean cattle) steers (n=14,386) was sampled from a commercial abattoir located in Seoul over one year period (spring, summer, autumn and winter) and their carcass traits were collected. Carcass traits assessed by an official meat grader comprised degree of marbling, meat color, fat color, texture and maturity for quality grade, and back fat thickness, ribeye area and carcass weight for yield grade. A heavier carcass with a higher marbling score, more red meat color and white fat color received better quality grade (p<0.05). Regression analysis showed that the marbling score was the strongest attribute (partial $R^2=0.88$) for quality grade. Lighter carcasses with a thinner back fat and larger ribeye area received higher yield grade score. The back fat thickness was the most negative determinant of yield grade (Partial $R^2=-0.66$). The slaughter season had a little effect on quality and yield grades. As slaughter weight increased, back fat thickness and ribeye area increased linearly, whereas marbling score reached its asymptotic level at approximately 570 kg. As a consequence, quality grade showed a considerable improvement up to 570 kg, but increases in slaughter weight afterward showed a little benefit on quality grade. There was a clear curvilinear relationship between slaughter weight and yield grade in that the yield grade reached its highest point at approximately 490 kg and decreased afterward. These results suggested that 570kg at the age of 24 months might be the economic slaughter weight for quality grade but 490 kg for yield grade.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.9
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• pp.1253-1260
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• 2017

Objective: Purpose was to determine the effects of grazing system and floor type on concentrations of blood metabolites, activity of creatine kinase, body weight and carcass characteristics of castrated Nguni goats. Methods: Forty eight, 7 month old goats were randomly allocated to herding and tethering treatments from 0800 to 1300 hours and accommodated on slatted and earth floors daily. Blood samples were collected by jugular venipuncture every fifteenth day for metabolite analysis. Slaughter was done at a commercial abattoir following 5 months of monitoring. Results: Tethered goats had significantly higher concentrations of urea (5.19 mmol/L) (p<0.001), creatinine ($55.87{\mu}mol/L$) (p<0.05), total protein (64.60 g/L) (p<0.01), and globulin (49.79 g/L) (p<0.001), whereas herded goats had higher glucose (3.38 mmol/L) (p<0.001), albumin (15.33 g/L) (p<0.05), albumin/globulin ratio (0.34) (p<0.01), and body weight (24.87 kg) (p<0.001). Slatted floors caused higher (p<0.01) albumin at 15.37 g/L. The interaction of grazing system and floor type affected creatinine, total protein, globulin at (p<0.01) and albumen/globulin ratio at (p<0.01). The least creatinine concentration and albumin/globulin ratio was in herded and tethered goats that were accommodated on earth floors, respectively. The highest total protein and globulin concentrations were in serum of tethered goats that were accommodated on earth floors. The highest (p<0.05) dressing percentage (45.26%) was in herded goats accommodated on slatted floors. Conclusion: Herding of goats lowered globulin concentration, improved estimated feed intake, blood glucose and albumin concentrations, albumin globulin ratio, increased body weights and weight related carcass characteristics. Floor type had very little effects on metabolites where earth floors only reduced albumin concentration. Tethering and housing goats on earth floors resulted in double stress that increased chronic infections.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.128-128
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• 2003

The aims of this study are 1) to test oocytes and embryos collected from in-vivo and in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to the procedures of Funahashi et al. Fertilized oocytes were cultured in glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39$^{\circ}C$, and 10% fetal bovine serum was added to the culture medium thereafter. Embryos were treated with 7.5$\square$g/ml cytochalasin-B for 30 min, centrifuged at 13,000 ${\times}$ g for 13 min and then exposed sequentially to an ethylene glycol (EG) vitrification solution, aspirated into OPSs, and plunged/thawed into/from liquid nitrogen. In vivo embryos were surgically collected from three donors after Al. Forty-six embryos (18, 9 and 19 embryos, respectively) were washed 3 times in mPBS+10%FBS, followed treatments : cultured, centrifuged, vitrified, recovered and transferred to recipients as in vitro prepared embryos. Three recipients received surgically 34(control), 188 and 184 embryos (derived from abattoir), respectively. Another three recipients were received nonsurgically 150, 100 and 150 embryos, respectively. All recipient sows exhibited delayed returns to estrus. To our knowledge, these results suggest that required an improved techniques, more vigorous embryos preparation and cleaner uterous condition(use gilt).

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.109-117
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• 1993

This paper dealt with the situation and the hatch rate of bot's eggs on the equine hairs in Cheju horse with the species grouping of the bot flies of equine(genus Gasterophilus). The prevalence and infection dynamics of Gasterophilus spp. larvae was also evaluated in the equine alimentary canal submitted in this laboratory for the necropsy or from the abattoir. Samples including Gasterophilus spp. larvae, bot's flies and its eggs, which were collected from the alimentary canal and equine hairs, respectively, were studied and classified by morphology. The morphologic feature of the spines of Gasterophilus larvae were studied by scanning electron microscope. 1. Gasterophilus intestinalis larvae concentrated in the nonglandular portions of the stomach. The infection of second-, and third stage larvae were common in November, and from January to October, respectively. Gasterophilus nasalis larvae were commonly identified on the gastric pylorus and upper portion of duodenum. Second stage larvae were found from October to December, and 3rd stage larvae, from January to September. 2. The hatch rate of laid eggs of Gasterophilus intestinalis was 28.4%, and that of Gasterophilus nasalis was 79.5%. The hatch rate of Gasterophilus intestinalis eggs was highest(62.5%) in December. The hatch rate of laid eggs were higher in the region of scapula(64.0%) and limbs(62.5%) than on the maned hairs. The eggs of Gasterophilus nasalis were completely hatched by October. 3. Eight hundred five Cheju horses examined in this study were infected with the eggs of Gasterophilus spp. Gasterophilus intestinalis eggs on the body regions from sixty horses were recognized in phalangeal (14.4%), in abdominal(13.8%), metacarpal, brachial and cervical regions. Gasterophilus nasalis eggs were uncommon and recognized in submandibular regions(1.4%). 4. In conclusion, the infection of imago, larvae and eggs of both Gasterophilus intestinalis and Gasterophilus nasalis were indentified in cheju horse.

• Parasites, Hosts and Diseases
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• v.56 no.2
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• pp.121-127
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• 2018

To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately $0.21-1.25{\mu}m$ in length and $0.05-0.07{\mu}m$ in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.842-849
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• 2007

The purpose of the present study was to produce live offspring from in vitro fertilized goat embryos. Oocytes were collected from abattoir ovaries and kept in oocyte collection medium. Oocytes were washed 4-5 times with maturation medium containing medium-199 with 5 ${\mu}g/ml$ FSH, 100 ${\mu}g/ml$ LH, 1 ${\mu}g/ml$ estradiol-$17{\beta}$ 50 ${\mu}g/ml$ gentamycin, 10% inactivated estrus goat serum, and 3% BSA (fatty acid free). Oocytes were placed in 100 ${\mu}l$ drops of maturation medium containing granulosa cell monolayer and incubated in a 5% $CO_2$ incubator at $38.5^{\circ}C$ for 27 h. For capacitation of spermatozoa fresh semen was processed and mixed in 3 ml fertilization TALP medium containing 50 ${\mu}g/ml$ heparin and kept in the above incubator for 2 h. The capacitated spermatozoa were coincubated with matured oocytes for fertilization. Cleaved embryos were separated and cultured in embryo development medium with oviductal cells and 494 embryos were produced. Recipient goats were synchronized with two injections of 15 mg $PGF_{{2}{\alpha}}$/goat 10 days apart. Eighty early stage embryos were transferred into the uterotubal junction of 14 surrogate mothers using laparoscopy techniques. One recipient delivered twin kids, whereas another two recipients each.delivered a single kid The parentage of these kids was evaluated using highly polymorphic co-dominant microsatellites markers. From the present study, it was concluded that live goat kids can be produced from in vitro matured and fertilized goat embryos, to the best of our knowledge for the first time in India.

• Korean Journal of Veterinary Research
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• v.30 no.3
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• pp.271-275
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• 1990

The tests related to red cell fragility were performed. Samples of blood anticoagulated with heparin were obtained from Korean native cattle in Chonbuk region abattoir, and classified by the district(Kun) with reference to breeding location. Hemolysis test for red cell fragility was performed with whole blood and glutathione peroxidase activity was measured spectrophotometrically. Blood concentration of selenium, inorganic component of glutathione peroxidase, was also determined fluorophotometrically. The results obtained were summerized as follows; 1. Percent hemolysis of erythrocytes ranged from 13.53 to 20.74%, and its mean Palue was low as $17.11{\pm}9.91%$. Means in all were not district(Kun) in Chonbuk region significantly different. 2. Glutathione peroxidase activity ranged from 2,881 to 4,000mU/ml, and high mean values, $3,352{\pm}1,872mU/ml$, reflected low percent hemolysis. 3. There was a highly negative correlation between the red cell fragility(Y) and blood glutathione peroxidase activity(X). The linear regression equation for these data was: Y=29.86-3.75X with a correlation coefficient of r=-.6886 (p<0.01) 4. Blood selenium concentration ranged from 0.16 to $0.24{\mu}g/ml$, and mean values was normal level as $0.2{\pm}0.11{\mu}g/ml$. 5. There was a highly positive correlation between blood selenium concentration(X), and blood glutathione peroxidase activity(Y). The linear regression for these data was: Y=230+15,790X, with a correlation coefficient officient of r=0. 8635.