• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.238-247
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• 2018

This study investigated survival rates and physiological responses in Pacific abalone (Haliotis discus hannai) to 18 days of containment in live fish containers ($8^{\circ}C$, 34 psu). The investigation was divided into three periods: before, during, and recovery after transportation. The overall survival rate was greater than 99%. Glucose, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) rose immediately on the first day of containment, but then gradually returned to normal levels. $NH_3$ continued to rise after the first day, but during the recovery period it decreased to a level not significantly different from that of the control group. $Na^+$ and osmolality did not show any abnormal changes. After recovery, superoxide dismutase (SOD) was not significantly different from control. Abalone in the experimental group had lower glutathione reductase (GR) than control. The hyalinocyte ratio fell immediately after confinement, but then gradually increased until it reached a normal level. The ratios of apoptotic and necrotic cells indicated no specific variations in hemocyte viability. Histological changes in the epidermal layer and muscle layer of the foot were not significantly different from those seen in the control group. The experimental data obtained in this study suggest that live fish containers may be used for transport of Pacific abalone without significantly impacting their physiology or survival rates.

• Korean Journal of Environmental Biology
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• v.25 no.2
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• pp.107-114
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• 2007

Radiation at very low doses frequently has a stimulating or hermetic effect on growth of organism. Effects of growth and survival rate on various dose of gamma irradiation in the farm culture of juvenile abalone (Haliotis discus hannai) were determined in Hallim (Jeju) from February 2004 to January 2005. The initial shell length of abalone juveniles in this study was average $3.45{\pm}0.4cm$. A change of growth after irradiation $(0{\sim}20Gy)$ was observed for 48 weeks. The highest growth rate was observed in 4 Gy-irradiated group and the lowest growth rate was observed in 20 Gy-irradiated group. The additional research about biochemical changes on juvenile abalone after irradiation should be accomplished. Continuous study for gamma radiation-induced hormesis on growth and metabolism of juvenile abalone will further induce the creation of value in ocean industry.

• Applied Biological Chemistry
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• v.27 no.1
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• pp.1-6
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• 1984

The lipoprotein-bound carotenoids occurred in the viscera of abalone (Haliotis discus hannai) were separated and characterized to be the mixture of fatty acid esters of lutein and free lutein. The main fatty acid components of the esters were lauric (68%) and myristic acid (17%), and indicated the characteristic pattern compared with that of triglycerides and cholesterol esters in abalone. Patterns of carotenoids from the lipoproteins were, simple and this suggested selected metabolisms of various feed-originated carotenoids found in the whole acetone extract of abalone.

• Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.81-88
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• 2015

We investigated factors affecting primary cultures of Pacific abalone Haliotis discus hannai ovary-dissociated cells to identify general aspects of their early-phase culture. Ninety-seven cell populations derived from 30 individuals were cultured in different media with varying compositions of medium supplements, and initial attachment, subculture, and survival for ${\geq}10$ weeks were assessed according to medium composition and individual. We also examined the time required for subculture and the rate of cell death according to both culturing period and passage number within 10 weeks. A lack of fetal bovine serum (FBS) and hemolymph significantly inhibited the growth of cultured cells, while we detected no significant effect of medium composition on initial cell attachment. Through data reallocation, with the omission of data from cell populations cultured in FBS-free and hemolymph-free media, we showed that growth inhibition was also affected by individual differences among the abalones used. During the culture, we observed four different types of cell morphology. Moreover, considerable time was required for subculture-18.4 and 19.5 days for first and second subcultures, respectively-and cell death did not occur within 30 days or for passage 0. Our results will provide valuable information for developing universal cell culturing guidelines in abalone species and suggest the feasibility of culturing abalone ovary-dissociated cells.

• Journal of Practical Agriculture & Fisheries Research
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• v.16 no.1
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• pp.143-154
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• 2014

Settlement of artificially released abalone, Haliotis discus hannai at the marine farming area in Jeollanam-do in 2007 was very efficient by showing the recapture of 2.0-5.2 ind./m² and the survival rate of 60-67%. The growth of shell after 1 year was highest in Hwoenggando by 75.9 mm and the lowest in Hwasan by 64.6 mm. Our result especially showed that the effect of the stock recruitment was the most efficient when abalone was released. The shell growth of the released abalone was better when sea mustard (Undaria sp.) was provided as food (shell length was up to 23.9 mm) or predators were periodically removed (shell length was up to 23.8 mm) in order to enhance the effect of the artificial release than the control group. The recapture rate was also higher than the control. However due to the difficulty of managing the release areas, it will be more efficient to release the recruited stocks after considering various biological and ecological factors such as survey of suitable release areas, stock size, release density, and health of stocks.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.317-322
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• 2011

This study was performed to obtain the basic data on physiological responses of low water temperature stress of the cultured Pacific abalone, Haliotis discus hannai. Abalones were exposed at low water temperatures of $7^{\circ}C$ and $4^{\circ}C$. We have investigated survival rate, superoxide dismutase (SOD) activity and total protein (TP) in the abalone by the exposure times (0, 3, 6, 12, 24, 72, 120, 168 and 216 hours). Survival rate of the abalone at $7^{\circ}C$ experiment was 90.8%, whereas at $4^{\circ}C$ experiment was 0% after exposure 10 days. SOD activity was significantly increased until 12 hours after exposure to $4^{\circ}C$, and then was recovered to starting level after 24 hours. However, there was no significant difference between control ($12^{\circ}C$) and $7^{\circ}C$ experiments. TP was significantly increased until 216 hours after 24 hours at $4^{\circ}C$ experiment, but $7^{\circ}C$ experiment showed no significant differences compared to control ($12^{\circ}C$) experiment. Therefore, H. discus hannai was acclimated in low water temperature stress at $7^{\circ}C$, but at $4^{\circ}C$, all abalone died possibly because they exceed the limits of defense ability to too low temperature.

• The Korean Journal of Malacology
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• v.32 no.2
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• pp.111-118
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• 2016

This study was conducted to find out concentration of trace metal, survival rate, falling rate and structural changes of the abalone Haliotis discus hannai exposed to nickel chloride. Experimental groups were composed of one control condition and four nickel chloride exposure conditions (7.0, 12.0, 17.0 and $22.0\;NiCl_2\;mg/L$). The concentration of nickel in abalone was significantly increased all exposure groups. Though the exposure groups had lower survival rate than the control group, falling rate higher than the control group. Histopathological changes in the foot, gill and hepatopancreas of abalone was revealed prominently with exposure group than control group.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.195-201
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• 2015

This study was conducted to find out concentration of trace metal, survival rate, falling rate and structural changes of abalone, Haliotis discus hannai exposed to zinc chloride. Experimental groups were composed of one control condition and four exposure conditions (0.3, 0.8, 1.3, $1.8\;ZnCl_2\;mg/L$). The accumulation of trace metal (zinc) in abalone was significantly increased all exposure group. Though the exposure groups had lower survival rate than the control group, higher falling rate than the control group. Histopathological changes in the foot, gill and hepatopancreas of abalone revealed obvious with exposure group than control group.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.5
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• pp.565-570
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• 2013

Physiological studies on the salinity tolerance with respect to survival rate and body composition changes of the abalone Haliotis discus hannai were conducted by rapidly changing the salinity in an indoor rearing system. The survival rate of the control (35 psu), 30 and 25 psu groups at $15^{\circ}C$ was 100%. The survival rate of the 20 psu group was $35{\pm}5.0%$. Survival rate of the 15 psu group was 0%. At $15^{\circ}C$ and 35, 30, 25, 20, 15 psu, the moisture contents of abalone muscle were $82.1{\pm}0.7$, $82.5{\pm}0.7$, $84.9{\pm}0.5$, $86.9{\pm}0.3$ and $86.6{\pm}0.4%$, respectively. Crude lipid contents were $0.47{\pm}0.03$, $0.47{\pm}0.03$, $0.47{\pm}0.09$, $0.77{\pm}0.09$ and $0.63{\pm}0.03%$, respectively and crude ash contents were $1.30{\pm}0.12$, $1.33{\pm}0.15$, $1.13{\pm}0.23$, $1.87{\pm}0.15$ and $1.40{\pm}0.31%$, respectively. At salinity below 20 psu, these values increased compared with the control. The general components of abalone muscles significantly increased below 20 psu, while amino acid composition showed no significant difference with salinity and water temperature.

• The Korean Journal of Malacology
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• v.31 no.2
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• pp.143-150
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• 2015

This study was conducted to find out accumulated concentration of nickel, survival rate and falling rate, structural changes of abalone, Haliotis discus hannai exposed to nickel chloride. Experimental groups were composed of one control condition and five nickel chloride exposure conditions (5.9, 8.8, 13.3, 20.0, 30.0 mg/L). The accumulation of nickel in abalone was significantly increased all exposure group. Though the exposure groups had lower survival rate than the control group, higher falling rate than the control group. Histopathological changes in the foot, gill and hepatopancreas of abalone revealed obvious with exposure group than control group.