• Title/Summary/Keyword: ASB

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Synthesis of the Low-Temperature Sintered Alumina Ceramic Composite(I) (저온소결용 알루미나 세라믹스 복합체 합성(I))

  • 김병익
    • Journal of the Microelectronics and Packaging Society
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    • v.5 no.1
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    • pp.19-30
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    • 1998
  • Aluminium secondary butoxide(ASB)를 출발물질로 하여 졸-겔방법에 의해 소결거 동에 미치는 $\alpha$-Al2O3 seed의 첨가효과에 따른 알루미나의 저온소결 가능성과 알루미나의 상전이에 대하여 TEM, DTA, XRD, FT-IR등으로 고찰을 하였다. TEM 분석결과 초기 생 성물인 boehmite가 비정질에서 단결정질로 진행되어 가고 있음을 확인하였다. 그리고 DTA 분석결과 $\alpha$-Al2O3 seed의 첨가한 경우 seed의 함량이 증가함에 따라 상전이 온도는 점차 낮아졌으며 약 0.4wt%일 때 seed를 첨가하지 않은 시료의 전이온도(약 1126$^{\circ}C$)에 비하여 약 7$0^{\circ}C$ 저하된 약 1056$^{\circ}C$로나타났으며 그 이상의 seedcja가에 있어서는 전이온도에 크게 영향을 나타내지 않았다. 또한 XRD분석결과 $\alpha$-Al2O3 seed를 첨가하지 않은 경우 110$0^{\circ}C$이 상의 온도에서 $\alpha$상이 생성되었음을 알수 있었다. 또한 100$0^{\circ}C$이상의 온도에서 $\alpha$상이 생성 되었음을 나타내는 Al-O 흡수특성 피크가 400~1000cm-1 범위에서 나타내고 있는 것을 FT-IR 분석결과에서도 확인할수 있었다. 그리고 $\alpha$-Al2O3 seed를 약 0.4wt% 첨가시 900~ 95$0^{\circ}C$에서 $\alpha$상이 형성됨을 관찰할 수 있었다.

Isolation of Streptomyces sp. YU100 Producing Extracellular Phospholipase D

  • Lim, Si-Kyu;Choi, Jae-Woong;Lee, Eun-Tag;Khang, Yong-Ho;Kim, Sang-Dal
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.71-76
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    • 2002
  • Soil samples were screened for actinomycete strains capable of producing phospholipase D, and a strain, Streptomyces sp. YU100, showing a high transphosphatidylation activity was isolated. This strain secreted phospholipase D in a culture broth after 12 h of cultivation, and its productivity continued to increase for 36 h of fermentation. In addition, its transphosphatidylation rate of phosphatidylcholine to phosphatidylserine was almost $68\%$ within 1 h. The morphological and chemotaxonomical characteristics showed that this strain could be classified as a number of the Streptomycetaceae family, particularly due to the spiral form of its spore chain consisting of 60-70 smooth spores $(0.75{\times}1.0{\mu}m$) on an aerial mycelium, FA-2c type of fatty acid profile in the cell wall, and LL-DAP component in the cell wall peptidoglycan. A phylogenetic analysis of the 16S rDNA provided a clue that the strain YU100 was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyes sp. ASB27, S. peucetius JCM9920, and S. griseus ATCC10137. A dendrogram based on the 16S rDNA sequences also showed a phylogenetic relationship between the strain YU100 and these strains. However, the strain YU100 has not yet been assigned to a particular species, because of absence of any other classified species with a high matching score.

칼슘락테이트가 반죽발효와 빵의 품질 및 저장성에 미치는 영향

  • 이예경;이명예;김순동
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.121.2-122
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    • 2003
  • 다슬기분말(PSB)과 그 회분(ASB)으로 제조한 칼슘락테이트(PCaL 및 ACaL)를 0.5%씩 첨가한 반죽의 발효와 빵의 품질 및 저장성에 미치는 영향을 조사하였다. 반죽의 pH는 4.85~4.98로 ACaL.PCaL.대조군의 순으로 나타났다. 반죽의 부피와 빵의 loaf volume index는 대조군이 높았고 ACaL 첨가군이 낮았으며, pH를 5.50으로 조정하여 제조한 반죽의 부피와 빵의 loaf volume은 대조군과 큰 차이를 보이지 않았다. PCaL 및 ACaL을 첨가한 빵의 Ca함량은 29.4~29.7 mg/100 g-f.w로 대조군의 13.0 mg/100 g-f.w에 비하여 높았으며, 첨가군의 미량 무기질로 Mg, Fe, Zn이 0.03~0.98 mg/100 g-f.w 범위로 검출되었다. 빵의 L$^{*}$ 값은 대조군과 실험군의 유의적인 차이가 없었으며, a*값, b*값은 PCaL 첨가군이 가장 높아 황갈색을 띄었다. 빵의 hardness, gumminess는 대조군$^{\circ}C$ 실온에 두면서 저장한 결과 대조군은 3일째부터, ACaL 첨가군은 5일째부터, PCaL 첨가군은 6일째부터 곰팡이가 번식하였다.

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Genetic Relationship among the Korean Native and Alien Horses Estimated by Microsatellite Polymorphism

  • Cho, G.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.784-788
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    • 2006
  • Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean native horse (102) and Jeju racing horse (56) together with Japan Hokkaido horse (5), Mongolian horse (19), Thoroughbred horse (108), Quarter horse (11) and Przewalskii horse (4). Allelic frequencies, the number of alleles per locus were estimated by direct counting from observed genotype, and genetic variability was computed using the CERVUX software and DISPAN. The number of alleles per locus varied from 6 (HMS6) to 18 (ASB17) with an average value of 10.45 in horse breeds. The expected total heterozygosity ($H_T$) and coefficient of gene differentiation ($G_{ST}$) ranged 0.764-0.921 (the average value was 0.830) and 0.102-0.266 (the average value was 0.180) in horse breeds, respectively. Four populations (Przewalskii horse, Japan Hokkaido horse, Quarter horse, Thoroughbred horse) showed lower heterozygosity than the average value (the average value was 0.710). The expected heterozygosity within breed ($H_S$) and mean no. of observed alleles ranged from $0.636{\pm}0.064$ (Japan Hokkaido horse) to $0.809{\pm}0.019$ (Mongolian horse), and from 2.73 (Przewalskii horse) to 8.27 (Korean native horse), respectively. The polymorphic information content (PIC) ranged from 0.490 (Przewalskii horse) to 0.761 (Mongolian horse) with an average value of 0.637 in horse breeds. The results showed three distinct clusters with high bootstrap support: the Korean native horse cluster (Korean native horse, Mongolian horse), the European cluster (Przewalskii horse, Thoroughbred horse), and other horse cluster (Jeju racing horse, Japan Hokkaido horse, and Quarter horse). A relatively high bootstrap value was observed for the Korean native horse cluster and European cluster (87%), and the Korean native horse and Mongolian horse (82%). Microsatellite polymorphism data were shown to be useful for estimating the genetic relationship between Korean native horse and other horse breeds, and also be applied for parentage testing in those horse breeds.

A Study on the Utilization of Coal Ash as Earthwork Materials (석탄회의 토공재로서의 활용에 관한 연구)

  • 천병식;임해식
    • Geotechnical Engineering
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    • v.12 no.1
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    • pp.73-86
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    • 1996
  • In this study, the possibility of the utilization of coal ash as earthwork materials is investigated. For this purpose, some laboratory experiments were carried out. The samples used in these tests are fly ash(FA), bottom ash(BA), coal ash dropped into ash pond(FA:BA=8:2), and mixed coal ash(FA:BA=5:5), which were discharged as a by-product at Yong-Yeul thermoelectric power plant, and general road filling materials. And for the deformation analysis of coal ash reclamation ground, several hyperbolic model parameters were determined by triaxial compression test. As a result of this study, coal ash has excellent engineering properties such as strength parameters comparing with general soils of the same grain size, especially in case of being used as backfill materials and reclamation materials on soft ground, and coal ash is superior to general earthwork materials in engineering properties becasuse of self hardening behaveiour, light weight property, etc.

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High Performance Ku-band 2W MMIC Power Amplifier for Satellite Communications (위성 통신 시스템 응용을 위한 우수한 성능의 Ku 대역 2W MMIC 전력증폭기)

  • Ryu, Keun-Kwan;Ahn, Ki-Burm;Kim, Sung-Chan
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.11
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    • pp.2697-2702
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    • 2014
  • In this paper, we demonstrated a Ku-band 2W MMIC power amplifier for satellite communication applications. The device technology used relies on $0.25{\mu}m$ GaAs pseudomorphic high electron mobility transistor (PHEMT) of Wireless Information Networking (WIN) Semiconductor foundry. The 2W MMIC power amplifier has gain of over 29 dB and saturation output power of over 33.4 dBm in the frequency range of 13.75 ~ 14.5 GHz. Power added efficiency (PAE) is a 29 %. To our knowledge, this is the highest power added efficiency reported for any commercial GaAs-based 2W MMIC power amplifier in the Ku-band.

Clinical Features, Molecular Analysis, and Outcome of ERT in Korean Patients with Mucopolysaccharidosis Type VI (국내 6형 뮤코다당증의 임상 양상, 분자유전학적 특징 및 효소치료의 효과에 대한 고찰)

  • Wichajarn, Khunton;Kim, Jinsup;Yang, Aram;Sohn, Young Bae;Lee, Beom Hee;Yoo, Han-Wook;Cho, Sung Yoon;Jin, Dong-Kyu
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.16 no.1
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    • pp.24-33
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    • 2016
  • Mucopolysaccharidosis type VI (MPS VI) is a rare disease caused by the mutation of ARSB with prevalence range from 1/5,000 in northeast Brazil to 1/2,057,529 births in Czech Republic. In Asia, there is only one published figure in Taiwan of about 1/833,000 births. The exact prevalence in the Korean population is unknown, but we estimated the incidence of MPS VI is about 0.03/100,000 live births. Enzyme replacement therapy (ERT) with recombinant human Arylsulfatase B (rhASB) is a modality for the treatment of MPS VI that reduces the excretion of urine glycosaminoglycan (GAG) and improves joint motion, pulmonary function, and endurance. We presented the clinical features, molecular analysis and outcome of ERT in three Korean MPS VI patients. All patients had the typical characteristic clinical features of MPS IV. Short stature, dysostosis multiplex, corneal opacity and valvular heart disease were found at first presentation, while restrictive lung disease and carpal tunnel syndrome developed later in all patients. Molecular analysis demonstrated novel missense and nonsense mutation in the patients, including p.Ile 67Ser, p.Gly328Arg, $p.Arg191^*$, p.Asp352Asn, and p.Gly17Asp. After ERT, urine GAG was decreased in all patients. Skeletal involvement, corneal opacity, heart valve abnormalities and pulmonary function were not improved with ERT, but it had a better outcome on regarding joint motion and endurance. One patient underwent allogeneic bone marrow transplantation (BMT) prior to ERT, but their clinical response was not improved much after BMT. This study demonstrates clinical phenotypes and molecular analysis of the severe form of MPS VI in Korean patients.

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Genetic Relationship and Characteristics Using Microsatellite DNA Loci in Horse Breeds. (Microsatellite DNA를 이용한 말 집단의 유전적 특성 및 유연 관계)

  • Cho, Gil-Jae
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.699-705
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    • 2007
  • The present study was conducted to investigate the genetic characteristic and to establish the parentage verification system of the Korean native horse(KNH). A total number of 192 horses from six horse breeds including the KNH were genotyped using 17 microsatellite loci. This method consisted of multiplexing PCR procedure. The number of alleles per locus varied from 5 to 10 with a mean value of 7.35 in KNH. The expected heterozygosity and observed heterozygosity were ranged from 0.387 to 0.841(mean 0.702) and from 0.429 to 0.905(mean 0.703), respectively. The total exclusion probability of 17 microsatellite loci was 0.9999. Of the 17 markers, AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 marker have relatively high PIC value(>0.7). This study found that there were specific alleles, P allele at AHT5, Q allele and R allele at ASB23, H allele at CA425, S allele at HMS3, J allele at HTG10 and J allele at LEX3 marker in KNH when compared with other horse populations. Also, the results showed two distinct clusters: the Korean native horse cluster(Korean native horse, Mongolian horse), and the European cluster(Jeju racing horse, Thoroughbred horse). These results present basic information for detecting the genetic markers of the KNH, and has high potential for parentage verification and individual identification of the KNH.