Isolation of Streptomyces sp. YU100 Producing Extracellular Phospholipase D

  • Lim, Si-Kyu (Institute of Biotechnology, Yeungnam University) ;
  • Choi, Jae-Woong (Department of Pharmacy, Yeungnam University) ;
  • Lee, Eun-Tag (Institute of Biotechnology, Yeungnam University) ;
  • Khang, Yong-Ho (Institute of Biotechnology, Department of Industrial Microbiology. Yeungnam University) ;
  • Kim, Sang-Dal (Institute of Biotechnology, Department of Industrial Microbiology, Yeungnam University)
  • 발행 : 2002.02.01

초록

Soil samples were screened for actinomycete strains capable of producing phospholipase D, and a strain, Streptomyces sp. YU100, showing a high transphosphatidylation activity was isolated. This strain secreted phospholipase D in a culture broth after 12 h of cultivation, and its productivity continued to increase for 36 h of fermentation. In addition, its transphosphatidylation rate of phosphatidylcholine to phosphatidylserine was almost $68\%$ within 1 h. The morphological and chemotaxonomical characteristics showed that this strain could be classified as a number of the Streptomycetaceae family, particularly due to the spiral form of its spore chain consisting of 60-70 smooth spores $(0.75{\times}1.0{\mu}m$) on an aerial mycelium, FA-2c type of fatty acid profile in the cell wall, and LL-DAP component in the cell wall peptidoglycan. A phylogenetic analysis of the 16S rDNA provided a clue that the strain YU100 was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyes sp. ASB27, S. peucetius JCM9920, and S. griseus ATCC10137. A dendrogram based on the 16S rDNA sequences also showed a phylogenetic relationship between the strain YU100 and these strains. However, the strain YU100 has not yet been assigned to a particular species, because of absence of any other classified species with a high matching score.

키워드

참고문헌

  1. J. Microbiol. Biotechnol. v.11 Levan-producing Bacillus subtilis BS62 and its phylogeny based on its 16S rDNA sequence Choi, S.-H.;C. Sung;W.-Y. Choi
  2. Biochim. Biophys. Acta v.488 The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography Comfurius P.;R. F. Zwaal https://doi.org/10.1016/0005-2760(77)90120-5
  3. Chemical Methods in Bacterial Systematics, Society of Applied Bacteriology Technical Series no.20 Introduction to chemosystematics Goodfellow, M.;D. E. Minikin;M. Goodfellow(ed.);D. E. Minikin(ed.)
  4. Biotechnol. Letters. v.18 Phospholipase D production using immobilized cells of Streptoverticillium cinnamoneum Fukuda, H.;Y. Turugida;T. Nakajima;E. Nomura;A. Kondo https://doi.org/10.1007/BF00154628
  5. Lipid Analysis - A Practical Approach. Thin-layer chromatography Henderson, R. J.;D. R. Tocher;R. J. Hamilton(ed.);S. Hamilton(ed.)
  6. J. Biochem. v.83 Enzymatic determination of phospholipase D activity with choline oxidase Imamura, S.;Y Horiuti https://doi.org/10.1093/oxfordjournals.jbchem.a131960
  7. J. Biochem. v.85 Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose Imamura, S.;Y. Horiuti https://doi.org/10.1093/oxfordjournals.jbchem.a132334
  8. J. Ferment. Bioeng. v.79 Extracellular production of phospholipase D of Streptomyces antibioticus using recombinant Escherichia coli Iwasaki, Y.;N. Mishima;K. Mizumoto;H. N. Nakano;T. Yamane https://doi.org/10.1016/0922-338X(95)91254-3
  9. Biochim. Biophys. Acta v.1003 Conversion of phosphatidylcholine to phosphatidylserine by various phospholipase D in the presence of L- or D- serine Juneja, L. R.;T. Kazuoka;N. Goto;T. Yamane;S. Shimizu
  10. Chemical Methods in Bacterial Systematics, Society of Applied Bacteriology Technical Series no.20 Fatty acid and menaquinone analysis of actinomycetes and related organisms Kropenstedt, R. M.;M. Goodfellow(ed.);D. E. Minikin(ed.)
  11. Biochem. J. v.345 Phospholipase D: Molecular and cell biology of a novel gene family Liscovitch, M.;M. Czarny;G. Fiucci;X. Tang https://doi.org/10.1042/0264-6021:3450401
  12. Bergey's Manual of Systematic Bacteriology v.4 Streptomycetes and related genera Locci, R.;S. T. Williams(ed.);M. E. Sharp(ed.);J. G. Holt(ed.)
  13. Acta Psychiatr. Scand. v.81 Effects of phosphatidylserine therapy in geriatric patients with depressive disorders Maggioni, M.;G. B. Picotti;G. P. Bondiolotti;A. Panerai;T. Cenacchi;P. Nobile;F. Brambilla https://doi.org/10.1111/j.1600-0447.1990.tb06494.x
  14. Hewlett-Packard gas chromatography application note Miller, L.;T. Berger
  15. J. Biochem. v.125 Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum Ogino, C.;Y. Negi;T. Matsumiya;K. Nakaoka;A. Kondo;S. Kuroda;S. Tokuyama;U. Kikkawa;T. Yamane;H. Fukuda https://doi.org/10.1093/oxfordjournals.jbchem.a022282
  16. J. Biochem. v.78 Studies on phospholipases from Streptomyces: Ⅱ. Purification and properties of Streptomyces hachijoensis phospholipase D. Okawa, Y.;T. Yamaguchi https://doi.org/10.1093/oxfordjournals.jbchem.a130916
  17. Appl. Microbiol. v.6 Aguide for classification of Streptomycetes according to selected groups placement of strains in morphological sections Pridham, T. G.;C. W. Hesseltine;R. G. Benedict
  18. J. Microbiol. Biotechnol. v.11 Identification of Streptomyces sp. AMLK-335 producing antibiotic substance inhibitory to vancomycin-resistant enterococci Rhee, K.-H.;K.-H. Choi;C.-J. Kim;C.-H. Kim
  19. Mol. Biol. Evol. v.4 The neighbor joining method: A new method for constructing phylogenetic data Saitou, N.;M. Nei
  20. A Laboratory Manual Molecular Cloning Sambrook, J.;E. F. Fritsch;T. Maniatis
  21. Biosci. Biotech. Biochem. v.57 Purification and properties of phospholipases D from Streptomyces antibioticus Shimbo, K.;Y. Iwasaki;T. Yamane;K. Ina
  22. Agric. Biol. Chem. v.53 Two Streptomyces strains that produce phospholipase D with high transphosphatidylation activity Shimbo, K.;Y. Yano;Y. Miyamoto
  23. Agric. Biol. Chem. v.54 Purifieation and properties of phospholipase D from Streptomyces lydicus Shimbo, K.;Y. Yano;Y. Miyamoto
  24. J. Ferment. Bioeng. v.81 Continuous production of phospholipase D by Streptomyces lydicus D-121 immobilized with crosslinked chitosan bead Shinonaga, M.-A.;Y. Kawamura;K. Shimbo;T. Yamane https://doi.org/10.1016/0922-338X(96)80582-4
  25. Int. J. syst. Bacteriol. v.16 Method for the characterization of Streptomyces species Shirling, E. B.;D. Gottlieb
  26. Kor. J. Appl. Microbiol. Biotechnol. v.22 Screening of phospholipase D producing actinomycetes Shon, D.-H.;J.-Y. Shim;S.-H. Yoon
  27. Methods for General and Molecular Bacteriology Phenotypic characterization Smibert, R. M.;N. R. Krieg;P. Gerhardt(ed.);R. G. E. Murray(ed.);W. A. Wood(ed.);N. R. Krieg(ed.)
  28. J. Bacteriol. v.173 16S ribosornal DNA amplification for phylogenetic study Weisburg, W. G.;S. M. Barns;D. A. Pelletier;D. J. Lane https://doi.org/10.1128/jb.173.2.697-703.1991
  29. J. Gen. Microbiol. v.48 Use of a scanning electron microscope for the examination of actinomycetes Williams, S. T.;F. L. Davies https://doi.org/10.1099/00221287-48-2-171
  30. J. Gen. Appl. Microbiol. v.16 Taxonomic studies on coryneform bacteria Ⅱ. Principle amino acids in the cell wall and their taxonomic significance Yamada, K.;K. Komagata https://doi.org/10.2323/jgam.16.1_103
  31. J. Biol. Chem. v.242 Transphosphatidylation by phospholipase D. Young, S. F.;S. Freer;A. A. Benson