• Maxillofacial Plastic and Reconstructive Surgery
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• v.37
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• pp.2.1-2.9
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• 2015

Background: The aim of this study was to evaluate the effect of autogenous tooth bone as a graft material for regeneration of bone in vertical bony defects of the minipigs. Material and Methods: Six minipigs were used in this study. Four molars were extracted in the right mandibular dentition and sent to the Korea Tooth Bank for fabrication of autogenous tooth bone. Ten days later, each extraction site was implanted with MS Implant Narrow Ridge $3.0{\times}10mm$ fixture (Osstem, Seoul, Korea) after standardized 2mm-sized artificial vertical bony defect formation. Pineappleshaped Root-On type autogenous tooth bones were applied to the vertical defects around the neck area of the posterior three fixtures and the fore-most one was not applied with autogenous bone as a control group. Each minipig was sacrificed at 4, 8, 12 weeks after fixture installation and examined radiologically and histologically. Histological evaluation was done under light microscope with Villanueva osteochrome bone staining with semi-quantitative histomorphometric study. Percentage of new bone over total area (NBF) and bone to implant contact (BIC) ratio were evaluated using digital software for area calculation. Result: NBF were $48.15{\pm}18.02%$, $45.50{\pm}28.37%$, and $77.13{\pm}15.30%$ in 4, 8, and 12 weeks, respectively for experimental groups. The control group showed $37.00{\pm}11.53%$, $32.25{\pm}26.99%$, and $1.33{\pm}2.31%$ in 4,8,12 weeks, respectively. BIC ratio were $53.08{\pm}19.82%$, $45.00{\pm}28.37%$, and $75.13{\pm}16.55%$ in 4,8,12 weeks, respectively. Those for the control groups were $38.33{\pm}6.43%$, $33.50{\pm}29.51%$, and $1.33{\pm}2.31%$ in 4, 8, 12 weeks, respectively. Conclusion: Autogenous tooth bone showed higher score than control group in NBF and BIC in all the data encompassing 4,8,12 weeks specimens, but statistically significant only 12 weeks data in both NBF and BIC.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.1-7
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• 2001

This experiment was carried out to investigate the effects of shading treatments and harvesting methods on the growth of Eleutherococcus senticosus, which was known as the medicine of anticancer, anti-stress, hepatic detoxification activity, immunoactivity, and tonic. Light transmission ratio and air temperature were decreased as $40{\sim}64%$,$1.9{\sim}2^{\circ}C$, respectively, in 30% to 70% shading net treatment compared to non-shading. Fifty percentage shading net treatment was effective for the growth and yield of Eleutherococcus senticosus. During summer Eleutherococcus senticosus was growing under shading net treatment compared to non-shading. Tunnel type was more effective for growth than vertical type in shading method. 30cm length included 2 to 3 nodes cutting from soil surface was the most effective for branching stem length, plant height and yield as harvesting methods.

• Tunnel and Underground Space
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• v.27 no.4
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• pp.230-242
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• 2017

Seismic velocities measured from in-situ tests (n=177) and through rock core samples (n=1,035) are reviewed in light of construction standards, widely used standards as a first-hand approximation of rock classification solely based on seismic velocities. In-situ down hole tests and refraction survey for soft rocks showed seismic velocities of 1,400~2,900 m/s which is faster than those specified in construction standards. For moderate~ hard rocks, in-situ down hole tests and refraction survey showed 2,300~3,800 m/s which roughly corresponds with the range specified in the construction standards. A similar trend is also observed for seismic velocities measured from rock core samples. The observed differences between construction standards and seismic velocities can be explained in two ways. If construction standards are correct the observed differences may be explained with seismic velocities affected by underlying fast velocities and also possibly with selection of intact cores for velocity measurement. Alternatively, construction standards may have intrinsic problems, namely artificial discrete boundaries between soft rocks and moderate rocks, application of foreign standards without consideration of geologic setting and lack of independent verification steps. Therefore, we suggest a carefully designed verification studies from a test site. We also suggest that care must be exercised when applying construction standards for the interpretation and accessment of rock mass properties.

• Journal of Embryo Transfer
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• v.16 no.1
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• pp.35-40
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• 2001

Artificial insemination (AI) with frozen or cooled semen is widely used in commercial fields of cattle and pig. Little is known about characteristics of canine sperm after freezing or cooling. For both practical and commercial goal, the canine semen treated with cooling and freezing should be carried out to exam the fundamentals, including sperm motility, survivability and fertilizing capacity. The aim of this study, thus, was to identify the effects of extended exposure to 4$0^{\circ}C$ on canine semen by motility, survivability, acrosomal changes following different duration. Fifteen ejaculates collected by digital manipulation twice per week from 3 dogs (Shih-Tzu) were divided to 16 aliquots after adding Tris-egg yolk (TE) buffer formulated by our laboratory, and cooled from 37 to 4$^{\circ}C$, by ramp rate of 0.6$^{\circ}C$/min. Each sample was evaluated by their motility, survivability and the acrosomal status at 0h (control), 2h, 12h and 1 d~10 d, respectively. The motility of spermatozoa was graded to 6 levels using the modified method of Seager. The survivability of sperm was assessed using an epifluorescence microscope after Fert/Light (Mole-cular Probes Inc.) staining. To estimate the proportion of the spermatozoa of intact acrosome, 200 spermatozoa were assessed in randomly selected fields, using epifluorescence microscope after FITC/PSA (Sigma) staining. At 2 h after cooling, the motility of most spermatozoa were assessed to be grade 0 and 1. At 12 h, high number of sperm were in grade 0 to 1, however, it was significantly (P<0.05) lower than that of 2 h. From 1 d to 4 d, ~50% of sperm was assessed to grade 0 to 1. On day 7, a little sperm were in grade 0 to 1. No sperm showed motility on day 10. Sperm motility was rapidly reduced by the percent of 10% of grade 0 to 1. From 2 h to 6 h, the number of live sperm was 90% and the sperm chilled for 10 days lived>50%. Acrosomal intact of spermatozoa exposed to 4$^{\circ}C$ for 2 h was 51%, supposed the sperm of control was 100%. Our results suggest that 1) this is easy to transfer and preservation for short periods 2) AI can be used by semen chilled for 6-Day.

• Parasites, Hosts and Diseases
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• v.46 no.4
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• pp.285-288
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• 2008

To examine the infection status of freshwater fish with Gnathostoma spp. larvae in Myanmar, we purchased 15 snakeheads, Channa striatus, from a local market in a suburban area of Naypyidaw, the new capital city. Two larval gnathostomes were collected using an artificial digestion technique, and observed by a light microscope and a scanning electron microscope. The size of an intact larva was 2.65 mm long and 0.32 mm wide. The characteristic morphology of the larvae included the presence of a long esophagus (0.80 mm long), 2 pairs of cervical sacs (0.43 mm long), and a characteristic head bulb with 4 rows of hooklets. The number of hooklets in the 1st, 2nd, 3rd, and 4th row was 45, 48, 50, and 52, respectively. Based on these morphological characters, the larvae were identified as the advanced 3rd-stage larvae of Gnathostoma spinigerum. This is the first report of detection of G. spinigerum 3rd-stage larvae in the central part of Myanmar. Our study suggests that intake of raw meat of snakehead fish in Myanmar may result in human gnathostomiasis.

• Journal of Mushroom
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• v.17 no.4
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• pp.191-196
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• 2019

The edible ectomycorrhizal mushroom Amanita javanica is a valuable species protected by forest law in Korea. However, basic characterization data on its use as an important forest resource has been limited. This study was performed to determine mycelia growth characteristics of the domestically isolated Amanita javanica strain NIFoS 1267 on potato dextrose agar media under diverse culture conditions. Physical factors temperature, pH, and light, as well as chemical factors salts, heavy metals, and pesticides were examined for their effects on the growth of the mushroom strain. The mycelia of A. javanica strain exhibited optimal growth when cultured in dark at 30℃ in media with a pH of 5-6. Normal levels of growth were observed in media containing up to 2% saline. At a heavy metal ion content of 50 ppm, mycelial growth was not affected by arsenic ion but was affected by cadmium and lead ions. In the tests performed with two pesticides used in Korean forests, the growth of the mushroom strain was not affected by the presence of abamectin, but was inhibited in media containing acetamiprid, emamectin benzoate, or thiacloprid. These results are expected to facilitate artificial cultivation of A. javanica as a new commercial product.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.4
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• pp.317-326
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• 2011

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion.

• The Korean Journal of Zoology
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• v.8 no.2
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• pp.89-127
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• 1965

1. This report is on the outcome of observation made up the breeding process of Narcissus flycatcher, Muscicapa narcissina zanthopygia, using 15 ness-boxes at Seoul and Kwang Nung Experimental forest in 1964, and the relevant feeding habit was studied by the collar method of wrapping the necks of thenestlings for 50-60 minutes or more in some cases from 1963 to 1964. 2. The Narcissus flycatcheris a common summer resident throughout Korea arriving at the end of April. The earliest data of arrival of this species in Seoul, is April 26, 1964. They migrate southward from the later part of September to early part of October. 3. In a week after their arrival , they start to build nest after a brief courtship. Male and female prepare their nest ina nestboxes in 4 or 5 days. Nests are usually found in the moss, which provide definite frame-work. The inner side of the nest is usually composed of roots of Gramineae or leaves of pine, oak or other broad leave tree. 4. The laying period is generally from the later part of April to the middle of June : and the number of eggs laid is from 3 to 8 , therefore 5 in average : eggs are pink with traces of milky yellow blending of light auburn and orange red spots and chicken-egg-like in shape. 5.The incubation takes place for 7-12 days from the middle part of May to the middle of June, in the order of male and female . The hatching rate tends to increase in proportion to clutch size. 6. The feeding period usually lasts 15 days in average from the early part of June to the middle of July and the feeding urge tends to increase in proportion to brood size. 7. The animals invading into their nest-boxes are red ants, chipmunks, bees , barkbeetles and philippine red-tailed shirkes, etc. 8. On the basis of analysis covering 714 birds in 31 nests, the food they consumed during the nesting period of nestlings were mostly of animal matters composing of : insect larvae form-39. 88% insect adult form 36.26%, insect pupae-0.19%, spider-17.75% and other animal matters 5.92% , Besides a small amount of plant pieces were found . As to the insect larvae, Lepidoptera was the largest in the content followed by Hymenoptera , Orthoptera and Coleoptera. As to the insect adult, Diptera was the largest in the content followed by Coleoptera, and Hymenoptera. In view of forest protection, noxious insect was of 69.38%, useful insect 4.39%, and spider 17.75%. 9. The rich assortment of its food, the ability of acquiring various insect (adults as well as larvae), its destruction of great numbers of harmful insects , and its quick assimilation artificial nesting with the nest-boxes of 2.5-3cm entrance hole employed, allow us to conclude that the flycatcher may be successfully employed in the elimination of fores pests. The above results are only that part obtained near Seoul in the middle of Korea, however, if we would extend the scheme to other broad areas using similar techniques, the nest-boxes could not only be well used by the birds, but also it would help to prevent the increase of harmful. insects.

• The International Journal of Costume Culture
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• v.4 no.1
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• pp.25-33
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• 2001

This study is to analyze Korean-Chinese women's Costume in Yabian with factors such as reform opening-up, economic growth, change of social values, development of technology, more education opportunities and influence of mass media. It divides the 1990's into two halves. Photos, interview, observation and relevant literature were used for this study. Even though economy grew rapidly, they needed to purchase daily goods other than clothes. In a way that they preferred practicality, it showed somewhat it was still developing. The trend in the first half of the 1990's was characterized : first, Synthetic or artificial fibers and ready-made suits were popular ; second, there still remained the men's style ; third, they wore Tanrikoo(彈力袴), Tisingkoo(體型袴), Jiaotakoo(脚袴) because of its properties of activeness and comfort ; fourth, shoulders looked ore prominent with pads as they were used in Chungsanfoo(中山服) ; fifth, clothes in grey and dark blue was in fashion ; sixth, they wore hand-made knit vests and sweaters and often mountain-climbing gears for its light and warm quality ; seventh, along with opening-up, various materials and colorful clothes were in style, which satisfied women's desire for beauty. They decorated Hanbok(韓服), using sleeves with colors, golden ornaments, flower patterns embroidery, materials of different color in collar and tie and dyes of sleeves and skirts. In the first half of the 1990's when the opening-up was beginning, there were diverse styles and colors in fashion, yet not close to good quality. As the economy grew, the second half of the 1990's was characterized by good palate, individuality, favouritism on foreign goods, rapid change of fashion. For instance, fur coat and woolen fabric were favored. Economic growth led to abundance of cloth, dyes and decorative materials. In addition, people possessed more clothing and it was possible for them to wear both Hanbok and wedding dress in wedding ceremony. People placed their standards on competency and financial ability rather than ideology. Worship disappeared and individuality arose. Therefore, apparel functioned not only as protection but as suits with aesthetic purpose. This resulted in introduction of bold style, imitation of western countries and extension of use of Hanbok. With the help of mass media, transportation, telecommunication, contact with Korean company and civilians, Korean and western cultures, through Korea, were accepted. Change in structure of economic status caused excessive spending and more educational opportunities that enabled people to accept foreign culture quickly. Values moved onto new, beautiful and better characteristics. it was possible to have suits ready-made due to improvement of mass production and cotten, wool, linen, silk became popular owing to technology. New technology, the bases of mass consumption, increased possession of clothing and accelerated change of fashion. In summary, women's Costume in Yanbian were affected by the factors in economy, politics, culture contact as well as change of society and technology.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.101-104
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• 2016

Curvularia leaf blight of creeping bentgrass (Agrostis stolonifera) putting green by caused Curvularia trifolii was observed in Hapcheon, Korea. In July to September 2014, curvularia leaf blight developed on leaf blades of creeping bentgrass as small water-soaked lesions that subsequently turned into dark-colored, necrotic spots. The spots were expanded and became gray, grayish-brown, or light brown, circular to oblong lesions with purple to dark brown borders that often were surrounded by a yellow halo. The necrotic lesions coalesced, became irregular in shape and caused tip or complete blighting of the leaves. Blighted leaf blades appeared grayish-white to tan. The fungus was identified by morphological characters and 16S rDNA sequencing as C. trifolii. Conidia of the pathogen were short, with predominantly 3-septa, straight or often curved, with end cells frequently paler than intermediate cells. Size of the 3-septate conidia in culture are $26{\sim}28{\times}11{\sim}12{\mu}m$. Pathogenicity of the fungus was proved by artificial inoculation on the host. This is the first report of C. trifolii causing leaf blight on creeping bentgrass in Korea.