• Title/Summary/Keyword: ARS1

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Development and Validation of a Predictive Model for Listeria monocytogenes Scott A as a Function of Temperature, pH, and Commercial Mixture of Potassium Lactate and Sodium Diacetate

  • Abou-Zeid, Khaled A.;Oscar, Thomas P.;Schwarz, Jurgen G.;Hashem, Fawzy M.;Whiting, Richard C.;Yoon, Kisun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.7
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    • pp.718-726
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    • 2009
  • The objective of this study was to develop and validate secondary models that can predict growth parameters of L. monocytogenes Scott A as a function of concentrations (0-3%) of a commercial potassium lactate (PL) and sodium diacetate (SDA) mixture, pH (5.5-7.0), and temperature (4-37DC). A total of 120 growth curves were fitted to the Baranyi primary model that directly estimates lag time (LT) and specific growth rate (SGR). The effects of the variables on L. monocytogenes Scott A growth kinetics were modeled by response surface analysis using quadratic and cubic polynomial models of the natural logarithm transformation of both LT and SGR. Model performance was evaluated with dependent data and independent data using the prediction bias ($B_f$) and accuracy factors ($A_f$) as well as the acceptable prediction zone method [percentage of relative errors (%RE)]. Comparison of predicted versus observed values of SGR indicated that the cubic model fits better than the quadratic model, particularly at 4 and $10^{\circ}C$. The $B_f$and $A_f$for independent SGR were 1.00 and 1.08 for the cubic model and 1.08 and 1.16 for the quadratic model, respectively. For cubic and quadratic models, the %REs for the independent SGR data were 92.6 and 85.7, respectively. Both quadratic and cubic polynomial models for SGR and LT provided acceptable predictions of L. monocytogenes Scott A growth in the matrix of conditions described in the present study. Model performance can be more accurately evaluated with $B_f$and $A_f$and % RE together.

Isolation of an Autonomously Replicating DNA Sequence from Aspergillus nidulans

  • Jang, Seung-Hwan;Jahng, Kwang-Yeon
    • Journal of Microbiology
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    • v.37 no.2
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    • pp.51-58
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    • 1999
  • Using yeast, Saccharomyces cerevisiae, and the integrate vector system, we have isolated and characterized an autonomously replicating sequence (ARS) from Aspergillus nidulans. The DNA fragment, designated ANR1, is 5.0 kb in size and maintained free from the chromosome in S. cerevisiae. The YIplac211-ANR1 recombinant plasmid, which consists of sequences derived from the yeast integrative vector YIplac211 and 5.0 kb ANR1 fragment, showed a 104-fold enhancement in transformation efficiency over that found for YIplac211, and was easily recovered from the transformed yeast. Genetic analysis of transformants showed that YIplac21-ANR1 could be over 96% cured when cultured over 20 generations in complete medium and thus suggests that this sequence is mitotically unstable. In A. nidulans, recombinant plasmid PILJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANR1 showed a 170-fold enhancement in transformation efficiency compared to that of the integrative vector PILJ16.

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Case Series Reporting 4 Cases of Attention Deficit Hyperactivity Disorder (ADHD) Treated with Galgeunhwangryeonhwanggeum-tang based on Disease Pattern Identification Diagnostic System by Shanghanlun (『상한론(傷寒論)』 변병진단체계(辨病診斷體系)에 근거하여 갈근황연황금탕(葛根黃連黃芩湯) 투여 후 호전된 성인, 소아 ADHD 4례)

  • Hyo-joong Yun;Min-hwan Kim;In-sun Doo;Sung-Jun Lee
    • 대한상한금궤의학회지
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    • v.15 no.1
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    • pp.85-115
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    • 2023
  • Objectives : This study reports on the cases of four ADHD patients who treated with herbal medication based on Disease Pattern Identification Diagnostic System (DPIDS). Methods : Two children and two adults were diagnosed with ADHD according to DSM-V, and Galgeunhwangryeonhwanggeum-tang was administered. The parents of the two children completed the Korean ADHD Rating Scale (K-ARS) before and after treatment, while the other patients used the Korean ADHD Self Rating Scale (K-ASRS). The patients' somatic symptoms and other issues were evaluated through interviews. Results : In the first case, the K-ARS scale exhibited a change from 28 to 3. The patient demonstrated improvements in attention, concentration, and social behavior, along with the elimination of self-injurious behavior. In the second case, the K-ARS scale showed a change from 41 to 9. The patient in the third case demonstrated improved chronic fatigue, increased attention, work efficiency, and enhanced social skills, leading to maintaining employment for over six months for the first time in their life after the treatments. Additionally, the patient in the fourth case demonstrated improved work concentration and relief in nocturia. Conclusions : In this study, four patients exhibited improvements in symptoms of attention deficit, hyperactivity, and impulsivity before and after herbal treatment, leading to enhanced academic and social relationships. As the patients progressed in their health restoration, symptoms related to the genitourinary system also showed improvement.

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2-way Service Using URL-style Command in DMB (DMB에서 URL방식 명령을 통한 양방향 서비스)

  • Lee, Jeong-Joon;Bae, Byung-Joon;Yun, Jeong-Il;Noh, In-Woo
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2007.02a
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    • pp.29-32
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    • 2007
  • 지상파 DMB 상용 서비스가 시작된 지 1년이 경과함에 따라 DMB 시청자의 수가 급격히 증가하였다. 그러나 DMB와 융합된 새로운 서비스가 활성화가 활발하지 못하고, 따라서 여러 가지 부가 수익서비스가 창출되지 못하고 있다. 그 이유 중의 하나는 기존에 제안된 양방향 데이터 서비스들은 모바일 홈페이지로의 연결하는 방식이라, 시청자가 DMB 단말기의 작은 화면에서 단말기의 버튼이나 터치스크린을 사용하기 불편하고, 방송시청이 불편하다. 또한, 기존의 방송참여 방식인 ARS, SMS 방식을 포용하지 못한다. 본 논문은 기존의 시청자 참여 방식을 분석하고 기존 방식의 약점을 보안하여 URL 방식 명령을 통한 양방향 서비스를 제안한다. 이 방법은 작은 디스플레이를 통해 홈페이지 방식을 고집하지 않고, 기존의 ARS, SMS를 포용하며, 단순한 동작으로 시청자 참여가 가능하다. 본 논문이 제안하는 방법은 서비스 방식 및 구조에 따라 ARS 서비스를 제공하는 URL방식 명령, SMS 서비스를 제공하는 URL 명령, 서버응용 호출서비스를 위한 URL방식 명령(SERVER-URL), 클라이언트 응용호출 서비스를 위한 URL방식 명령(CLIENT-URL)으로 분류된다. 본 방법들은 사용자의 참여가 단순하게 이루어지도록 방송시 송출되며, 이러한 방법은 ol동 방송에 특히 적합할 것으로 사료되어, 앞으로 보다 실용적인 DMB 기반의 융합 서비스에 사용될 것으로 사료된다.

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Structure Analysis of ARS Cryptoprocessor based on Network Environment (네트워크 환경에 적합한 AES 암호프로세서 구조 분석)

  • Yun, Yeon-Sang;Jo, Kwang-Doo;Han, Seon-Kyoung;You, Young-Gap;Kim, Yong-Dae
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.15 no.5
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    • pp.3-11
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    • 2005
  • This paper presents a performance analysis model based on an M/M/1 queue and Poisson distribution of input data traffic. The simulation on a pipelined AES system with processing rate of 10 rounds per clock shows $4.0\%$ higher performance than a non-pipelined version consuming 10 clocks per transaction. Physical implementation of pipelined AES with FPGA takes 3.5 times bigger gate counts than the non-pipelined version whereas the pipelined version yields only $3.5\%$ performance enhancement. The proposed analysis model can be used to optimize cost-performance of AES hardware designs.

Separation of Fe(Ⅱ) from Co(Ⅱ), Ni(Ⅱ), Pb(Ⅱ) Using Amberlite IRA 400 Loaded with Alizarin Red S (Alizarin Red S가 결합된 Amberlite IRA 400 에 의한 Co(Ⅱ), Ni(Ⅱ), Pb(Ⅱ) 중 Fe(Ⅱ)의 분리)

  • Cha, Ki Won;Park, Chan Il;Kang, Ji Soo
    • Journal of the Korean Chemical Society
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    • v.42 no.2
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    • pp.172-176
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    • 1998
  • An anion exchange resin which has Alizarin Red S (ARS) as functional group was prepared by batch method. The resin is stable in acidic solutions below 0.5 M hydrochloric acid, nitric acid and sulfuric acid and adsorption capacity of Fe(Ⅱ) ion on the resin was larger than other metal ions. The preconcentration and the separation of Fe(Ⅱ) ion from the mixture solution were carried out with the pH 4.5 buffer solution and 0.1 M $HNO_{3}$ as eluents.

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Empirical Relations of Nutrients, N : P Ratios, and Chlorophyll in the Drinking Water Supplying Dam and Agricultural Reservoirs

  • Lee, Sang-Jae;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.41 no.4
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    • pp.512-518
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    • 2008
  • This study were to evaluate trophic conditions, N : P ratios, and empirical relations of chlorophyll (CHL) systematically using TN, TP, and CHL values in agricultural reservoirs and drinking water supplying dams. During the study, nutrients and CHL varied depending on seasonal conditions and types of the reservoirs, but most reservoirs were diagnozed as eutrophic to hypertrophic. Mass ratios of TN : TP averaged 93.1 (range: $0.68{\sim}1342$) and about 96.6 % of the total observations (n=516) was > 17 in the N : P ratios. This result suggests that P was a potential factor limiting algal growth in the entire reservoir. Thus, TN : TP ratios were a function of phosphorus rather than nitrogen. Regression analysis of log-transformed N : P ratios against TP in DWDRs and ARs showed that ratios were linearly declined with an increase of TP ($R^2$>0.66; p<0.001). Seasonal mean CHL was minimum ($4.3{\mu}g\;L^{-1}$, range: $0.1{\sim}39.7{\mu}g\;L^{-1}$) in premonsoon, and was similar between the monsoon and postmonsoon. In contrast, one of the tremendous features was that values of CHL was greater in the ARs than DWDRs. Thus, the spatial and temporal patterns in CHL were similar to those of TP but not TN. Empirical models of CHL-TP showed that CHL variation could explain average 15.3% and 11.3% in DWDRs and ARs, respectively. Seasonal analysis of empirical models showed that CHL-TP relations were stronger in postmonsoon than those of premonsoon and monsoon.