• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.278-284
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• 1994

The sulfur amino acid composition in soybean (Glycine max L.) seeds may be an essential characteristic of new cultivars for some animal diets. Variation in seed storage protein among genotypes might make it possible to improve the quality of seed protein by genetically altering seed storage protein composition through plant breeding. This study was carried out to determine if mutant strains have potential for improving seed protein quality in soybean. Ten mutant strains had a distinct characteristic of seed storage protein subunits. Among the mutant strains, the sulfur amino acid compositions(methionine plus cystein) of Keburi(P.I.417016), Keburi(P.I.506817), and P.I.54608-1 were relatively higher than those of the others and were 1.9, 2.1, and 1.8%, repectively, which might be due to low levels of ${\alpha}$, ${\alpha}$', and ${\beta}$ subunits of 7S protein. Therefore, it is concluded that the mutant strains, Keburi(P.I.417016), Keburi(P.I.506817), and P.I.54608-1 appear to be potential materials for a breeding program for improving sulfur amino acid composition, and the others also seem to be possible breeding materials for other uses.

• Journal of Biosystems Engineering
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• v.36 no.1
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• pp.33-39
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• 2011

An optical sensing approach based on diffuse reflectance has shown potential for rapid and reliable on-site estimation of soil properties. Important sensing ranges and the resulting regression models useful for soil property estimation have been reported. In this study, a similar approach was applied to investigate the potential of reflectance sensing in estimating soil properties for Korean paddy fields. Soil cores up to a 65-cm depth were collected from 42 paddy fields representing 14 distinct soil series that account for 74% of the total Korean paddy field area. These were analyzed in the laboratory for several important physical and chemical properties. Using air-dried, sieved soil samples, reflectance data were obtained from 350 to 2500 nm on a 3 nm sampling interval with a laboratory spectrometer. Calibrations were developed using partial least squares (PLS) regression, and wavelength bands important for estimating the measured soil properties were identified. PLS regression provided good estimations of Mg ($R^2$ = 0.80), Ca ($R^2$ = 0.77), and total C ($R^2$ = 0.92); fair estimations of pH, EC, $P_2O_5$, K, Na, sand, silt, and clay ($R^2$ = 0.59 to 0.72); and poor estimation of total N. Many wavelengths selected for estimation of the soil properties were identical or similar for multiple soil properties. More important wavelengths were selected in the visible-short NIR range (350-1000 nm) and the long NIR range (1800-2500 nm) than in the intermediate NIR range (1000-1800 nm). These results will be useful for design and application of in-situ close range sensors for paddy field soil properties.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.8
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• pp.662-669
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• 2012

The effect of aspect ratio (AR) on the aerodynamic characteristics of a flapping wing was examined to analyze the design parameters of an insect-based MAV. The experimental model constructed with 4-bar linkages was operated in a water tank with the condition of a low Reynolds number. A water-proof micro-force load cell was fabricated and installed at the root of the wing which is made of a plexiglas. The wing shapes were based on the planform of a fruit fly wing. The ARs selected were 1.87, 3.74 and 7.48 and the Reynolds number was fixed at $10^4$. For AR=1.87 and 3.74, distinct lift peaks which indicate unsteady effects such as 'wake-capture' were observed at the moment of the start of the wing-stroke. However, for AR=7.48, no unsteady effects were observed. These phenomena were also observed in the delayed rotation case. The results indicate that a larger AR provides better aerodynamic performance for the insect-based flapping wing which can be applied in MAV designs.

• Clinical and Experimental Pediatrics
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• v.60 no.6
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• pp.189-195
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• 2017

Purpose: The purpose of this study was to investigate the effects of lamotrigine for the treatment of attention-deficit hyperactivity disorder (ADHD) symptoms in children with epilepsy. Methods: Pediatric patients newly diagnosed with epilepsy (n=90 [61 boys and 29 girls]; mean age, $9.1{\pm}3.4years$) were enrolled. All patients were evaluated with the Korean ADHD rating scale (K-ARS)-IV before treatment with lamotrigine and after doses had been administered. The mean interval of ADHD testing was approximately 12.3 months. The initial dosage of lamotrigine was 1 mg/kg/day (maximum 25 mg/day for the first 2 weeks), and increased by 1 mg/kg every 2 weeks until titrated up to 7 mg/kg/day (or maximum 200 mg/day). Results: The mean ADHD test score of the 90 subjects was $17.0{\pm}1.8$ at baseline. It was slightly reduced to $15.6{\pm}1.7$ after lamotrigine monotherapy (P>0.01). Prior to treatment, a total of 31 patients (34.4%) met the diagnostic criteria for ADHD according to Diagnostic and Statistical Manual of Mental Disorders, 4th Edition, Text Revision, Of these 31 patients, 27 (87.1%) had significantly improved ADHD scores with lamotrigine monotherapy ($28.0{\pm}1.6$ reduced to $18.1{\pm}2.6$, P<0.001). Among these 27 patients, 25 (92.6%) showed normalized electroencephalogram (EEG) and 26 (96.3%) achieved total freedom from seizures within 12 months of the initiation of lamotrigine monotherapy. Conclusion: The results from our study show that lamotrigine had a positive effect in pediatric epilepsy patients by reducing ADHD symptoms, preventing seizures, and normalizing EEG. However, further research is required to determine whether lamotrigine is efficacious against ADHD symptoms independent of its effects on epileptic seizures.

• Journal of The Korean Society of Agricultural Engineers
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• v.51 no.1
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• pp.41-52
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• 2009

새로 개발된 모형에 대한 민감도 분석은 입력변수의 선정 및 보정을 위한 필요한 지침을 제공하기 위해 수행되어야 할 필수적인 과정이다. 동적 농업비점오염 평가모델 (dynamic agricultural nonpoint source assessment tool, DANSAT)은 농업 최적관리기법(BMP)의 지표 및 지하수에 대한 영향을 평가하기 위해 개발된 3차원 모델이다. 본 연구의 목적은 서로 다른 토층 및 유역내 위치에서 입력변수의 변화에 따른 지표 및 지하관련 출력들의 반응을 토대로 DANSAT의 일반적인 민감도 분석을 수행하는 것이다. 선정된 입력변수들의 변화에 따른 세가지의 지표관련 출력 (유출량, 유사량, 유출수중 농약 부하량) 및 두가지의 지하관련 출력 (지하수로의 유입 유량 및 지하수로의 농약 유입량)의 반응이 고려되었다. 민감한 입력변수들은 하나의 격자만을 이용한 예비 민감도 분석을 통하여 선정되었는데, 대체로 토양 관련 인자들이 지표 및 지하에서 양적/질적으로 민감하게 작용하는 것으로 나타났다. 예비민감도 분석을 통해 선정된 토양입력변수들의 서로 다른 토층에서의 변화에 따른 모형 출력들의 반응을 고려한 수직적 민감도 분석결과, 지표관련 출력들의 경우 지표 부근의 상층 토양의 인자가, 지하관련 출력들의 경우 하부 토층의 인자가 각각 민감하게 작용하는 것으로 평가되었다. 유역내에서 입력변수들의 공간적 위치의 변화에 따른 반응을 고려하는 수평적 민감도 분석결과, 유역 경계의 주변보다 하천 주변에서의 입력변수 변화가 모형의 지표 및 지하관련 출력들에 민감한 것으로 나타났다. 본 연구 결과는 BMP의 지표 및 지하의 수문/수질에 미치는 영향을 평가하기 위해 개발된 DANSAT 모형의 입력변수 선정 및 보정에 유용한 자료로 활용될 것으로 기대된다.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.489-499
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• 2016

Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses, respectively. In this, 4, 4, and 2 Vitis species were classified into RR, SR, and SS, respectively. Gas chromatography mass spectrometry (GC-MS) analysis of the grapevine stem metabolites with A. vitis infection showed 134 metabolites in various compound classes critically occurred, which were differentially clustered with the response types by the principal component analysis. Multivariate analysis of the metabolite profile revealed that 11 metabolites increased significantly in relation to the response types, mostly at post-inoculation stages, more prevalently (8 metabolites) at two days after inoculation than other stages, and more related to SS (7 metabolites) than RR (3 metabolites) or SR (one metabolite). This suggests most of the disease-related metabolites may be rarely pre-existing but mostly induced by pathogen infection largely for facilitating gall development except stilbene compound resveratrol, a phytoalexin that may be involved in the resistance response. All of these aspects may be used for the selection of resistant grapevine cultivars and their rootstocks for the control of the crown gall disease of the grapevine.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.143-146
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• 2011

Some of men have been suffered from the insufficient secretion of testosterone causing by physical factors, social and psychological factors. Testosterone is an essential steroid hormone controlling male reproductive function. Alternative medicines in plants, fungi, and insects have been studied to enhance sexuality. $Cordyceps$ species including $Cordyceps$ $sinensis$ (CS) and $C.$ $militaris$(CM) has been used as for the enhancement of sexual functionfor hundreds of years in Far East Asian. In the present study, we determined the effect of fruiting bodies of $C.$ $militaris$ which cultured on bee drone medium (CMD) and brown rice medium (CMB) on testosterone concentration in Sprague-Dawley rats. Eighteen rats per group were housed to regular diet or diet supplemented with CMB and CDD, respectively for 4 weeks. Serum was collected from 6 rats per group. Results showed that changes of the body weight, food and water intake of the rats were not observed in this study. However, both CMB and CDD increased the serum testosterone concentration in rats. Furthermore, CMD significantly stimulated testosterone production (p <0.05) compared to the control. Hence, it suggests that $C.$ $militaris$ fruiting bodymight be developed as a complementary medicine to improve sexual hormones.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.1072-1076
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• 2004

도시 개발에 의해 우수의 불투수지역 확대, 하천부지의 축소, 산림 및 유수지의 감소 등이 급속히 진행되어 하천유량의 변화, 지하수위의 저하, 용수의 고갈, 생태계의 파괴 등이 발생되어 왔다. 도시지역은 도시형 수해발생, 갈수시의 급수안전도 지하, 평시 하천유량의 감소, 공공수역의 수질악화, 지하수 오열 등 여러 가지 문제에 직면하고 있다. 이러한 문제들은 서울의 경우도 예외는 아니며 청계천 복원 사업과 더불어 그동안 방치되었던 도시유역의 물순환 체계를 분석할 필요가 있다. 본 연구에서는 SWAT 모형을 이용하여 도시하천 유역의 물순환을 해석하였다. SWAT모형은 미국 농무성 농업연구소(Agricultural Research Service, ARS)에서 개발된 모델로서, 내규모의 복잡한 유역에서 장기간에 걸친 다양한 종류의 토양과 토지이용 및 토지관리 상태에 따른 물과 유사 및 농업화학물질의 거동에 대한 토지관리 방법의 영향을 예측하기 위해 개발되었다. SWAT 모형은 물리적 이론에 근거한 연속모형으로 준분포형 (Semi-Distributed) 모형이다. 본 연구는 도시하천 유역의 물순환체계 변동을 고려한 물순환 정상화 기술을 개발하기 위한 기초단계로서, 청계천 유역에 내해 모형을 적용하였다. 청계천은 중랑천의 제1지류인 지방2급 하천으로 유역면적 $50.96km^2$, 유로연장 13.75km이며, 2003년 7월부터 ,5.9km의 본류구간에 대한 복원공사가 진행 중이다. 적용유역의 수문${\cdot}$기상${\cdot}$지하수 자료는 1993널 1월 1일 $\~$ 2002년 12월 31일까지의 서울 기상청 자료를 이용하였으며, 지형, 토양, 토지이용 자료는 기존에 구축된 GIS 자료를 이용하였다. 모형 적용결과, 도시하천 유역에 대한 SWAT 모형의 적용성을 확인할 수 있었으며 유역의 물순환계를 구성하는 강수, 지표수, 토양수, 지하수 및 하천수 등의 상호 관계 분석을 통해 장기간의 유역 물순환체계 변화를 분석할 수 있었다.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.201-210
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• 2007

Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.