• Title/Summary/Keyword: AOTF

Search Result 28, Processing Time 0.046 seconds

Research about Hyperspectral Imaging System for Pre-Clinical testing of Small Animal (소형동물 전임상실험을 위한 하이퍼스펙트럼 영상장비 연구)

  • Lee, kyeong-Hee;Choi, Young-Wook
    • The Transactions of The Korean Institute of Electrical Engineers
    • /
    • v.56 no.12
    • /
    • pp.2208-2213
    • /
    • 2007
  • In this study we have developed a hyperspectrum imaging system for highly sensitive and effective imaging analysis. An optical setup was designed using acoustic optical tunable filter (AOTF) for high sensitive hyperspectrum imaging. Light emitted by mercury lamp gets split in to diffracted and undiffracted beams while passing though AOTF. GFP transfected HEK-293 cell line was used as a model for in vitro imaging analysis. Cells were first, analyzed by fluorescence microscope followed by flow cytometric analysis. Flow cytometric analysis showed 66.31% transfection yield in GFP transfected HEK-293 cells. Various images of GFP transfected HEK-293 cell were grabbed by collecting the diffracted light using a CCD over a dynamic range of frequency of 129-171 MHz with an interval of 3 MHz. Subsequently, for in vivo image analysis of GFP transfected cells in mouse, a whole-body-imaging system was constructed. The blue light of 488 nm wavelength was obtained from a Xenon arc lamp using an appropriate filter and transmitted through an optical cable to a ring illuminator. To check the efficacy of the newly developed whole-body-imaging system, a comparative imaging analysis was performed on a normal mouse in presence and absence of Xenon arc irradiation. The developed hyperspectrum imaging analysis with AOTF showed the highest intensity of green fluorescent protein at 153 MHz of frequency and 494 nm of wavelength. However, the fluorescence intensity remained same as that of the background below 138 MHz (475 nm) and above 162 MHz (532 nm). The mouse images captured using the constructed whole-body-imaging system appeared monochromatic in absence of Xenon arc irradiation and blue when irradiated with Xenon arc lamp. Nevertheless, in either case mouse images appeared clearly.

Hyperspectral Fluorescence Imaging for Mouse Skin Tumor Detection

  • Kong, Seong G.;Martin, Matthew E.;Vo-Dinh, Tuan
    • ETRI Journal
    • /
    • v.28 no.6
    • /
    • pp.770-776
    • /
    • 2006
  • This paper presents a hyperspectral imaging technique based on laser-induced fluorescence for non-invasive detection of tumorous tissue on mouse skin. Hyperspectral imaging sensors collect image data in a number of narrow, adjacent spectral bands. Such high-resolution measurement of spectral information reveals contiguous emission spectra at each image pixel useful for the characterization of constituent materials. The hyperspectral image data used in this study are fluorescence images of mouse skin consisting of 21 spectral bands in the visible spectrum of the wavelengths ranging from 440 nm to 640 nm. Fluorescence signal is measured with the use of laser excitation at 337 nm. An acousto-optic tunable filter (AOTF) is used to capture images at 10 nm intervals. All spectral band images are spatially registered with the reference band image at 490 nm to obtain exact pixel correspondences by compensating the spatial offsets caused by the refraction differences in AOTF at different wavelengths during the image capture procedure. The unique fluorescence spectral signatures demonstrate a good separation to differentiate malignant tumors from normal tissues for rapid detection of skin cancers without biopsy.

  • PDF

Optical performance monitoring in 16CH WDM system, using FFP-TF (FFP-TF를 활용한 OPM의 16CH WDM시스템 광 성능 모니터링)

  • 이동선
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.28 no.8A
    • /
    • pp.657-662
    • /
    • 2003
  • The OPM(optical performance monitoring module) is needed in order to monitor optical performance. the most importance of OPM is to measure the wavelength of optical signal. In the past time, it was very difficult to get the wavelength value because they used pilot tone. Since then, using AWG(arrayed waveguide grating) and AOTF(acousto optic tuneable filter), the wavelength and the transmission qualify for multi channel signal have been monitored. In this paper, we chose the fiber fabry-perot wavelength variable filter which be evaluated for optical resolution ability to excellent, so that FBG(fiber bragg grating) was used for setting reference wavelength because chose a wavelength variable filter.

All-fiber dynamic add-drop multiplexer based on acousto-optic interaction (음향광학 효과를 이용한 전광섬유 동적 파장가감기)

  • 박희수;송광용;윤석현;김병윤
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2002.07a
    • /
    • pp.52-53
    • /
    • 2002
  • 파장분할 다중화 광통신 선로 상에서, 특정 파장 채널을 임의로 선택하여 분리/첨가 할 수 있는 동적파장가감기(wavelength-division dynamic optical add-drop multiplexer)는 광 네트워크의 효율을 높이는데 핵심 소자라 할 수 있다. 지난 수년간 개발된 수많은 기술들 중에서, LiNbO$_3$ 광도파로 음향광학소자(acousto-optic tunable filter: AOTF)는 몇 가지의 특징적인 장점들을 보여줬는데, 예를 들면 넓은 영역의 파장 선택성, 짧은 스위칭 시간, 다중 채널 동작 가능성 등이다. (중략)

  • PDF

Enhancement of free spectral range in the resonance peaks in a long period fiber grating by controlling clad mode material dispersion (광섬유 클래딩 모드의 분산 특성 조절에 의한 장주기 격자 내에서의 공명 피크의 free spectral range 향상)

  • Jeong, Hoon;Oh, K.
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2000.02a
    • /
    • pp.72-73
    • /
    • 2000
  • Optical fiber gratings have been intensively studied for applications in optical fiber communications and sensor systems due to their all-fiber configuration, low insertion loss, low cost, and high flexibility. Especially, cladding mode coupling fiber devices such as long period gratings (LPG) and acousto-optic tunable filters (AOTF) have been developed as a gain equalization filter in erbium doped fiber amplifier (EDFA) for wavelength division multiplexing (WDM) systems$^{(1-2)}$ . (omitted)

  • PDF

A Study on the Acousto-Optical Wavelength Tunable Filters Utilizing a SAW Guide Variable Directional Coupler (방향성 가변결합 구조의 음향파 도파로를 이용한 음향광학형 파장가변 필터에 관한 연구)

  • 정기조;임경훈;정흥식
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2001.02a
    • /
    • pp.152-153
    • /
    • 2001
  • 파장 가변형 광 필터와 파장 선택형 광 스위치는 파장분할다중 광통신 및 광 교환 시스템을 구현하는데 매우 중요한 소자들 중의 하나이다. 특히 음향광학효과를 이용한 파장 가변형 광 필터(AOTF : Acousto-Optic Tunable Filter)는 150nm 이상의 넓은 파장 가변범위, 1.5nm이하의 좁은 통과대역폭, 수 $\mu\textrm{s}$ 정도의 비교적 빠른 스위칭 속도 그리고 여러 개의 파장 채널을 동시에 선택할 수 있는 장점들을 가지고 있지만, 한편으로 표면 음향파(SAW: Surface Acoustic Wave) 구동에 필요한 RF 파워와 부 모드 (sidelobe)가 비교적 크다는 단점 때문에 실용화에 많은 제약을 받아왔다. (중략)

  • PDF

A Study of the Effect of Sidelobes on Multichannel Operation Characteristics of $LiNbO_3$ Acousto-Optical Tunable Wavelength Filters ($LiNbO_3$ 음향광학효과형 가변파장 광 필터의 다채널 특성에 부 모드가 미치는 영향에 관한 연구)

  • 김정희;정홍식
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2001.02a
    • /
    • pp.154-155
    • /
    • 2001
  • 파장가변 광 필터는 WDM 광전송 시스템에 필요로 하는 핵심 부품중의 하나이다. 특히 LiNbO$_3$의 음향광학효과를 이용하는 AOTF(Acousto-Optic Tunable Filter)는 150nm 이상의 파장 가변 범위, 1.5nm 이하의 파장 통과대역폭, 수 $\mu\textrm{s}$ 정도의 비교적 빠른 스위칭 속도, 그리고 여러 파장 채널을 동시에 선택할 수 있는 장점들을 보유하고 있음에도 불구하고 표면 음향파(SAW: SurFace Acoustic Wave) 구동에 필요한 RF 파워와 부 모드(sidelobe)가 비교적 크다는 단점 때문에 실용화에 많은 제약을 받아왔다. (중략)

  • PDF

Scientific and Engineering Applications of Full-field Swept-source Optical Coherence Tomography

  • Mehta, Dalip Singh;Anna, Tulsi;Shakher, Chandra
    • Journal of the Optical Society of Korea
    • /
    • v.13 no.3
    • /
    • pp.341-348
    • /
    • 2009
  • We report the development of full-field swept-source optical coherence tomography (SS-OCT) in the wavelength range of 815-870 nm using a unique combination of super-luminescent diode (SLD) as broad-band light source and acousto-optic tunable filter (AOTF) as a frequency-scanning device. Some new applications of full-field SS-OCT in forensic sciences and engineering materials have been demonstrated. Results of simultaneous topography and tomography of latent fingerprints, silicon microelectronic circuits and composite materials are presented. The main advantages of the present system are completely non-mechanical scanning, wide-field, compact and low-cost.