Kim, Kwan-Su;Park, Chun-Geun;Kim, Dong-Hwi;Park, Si-Hyung;Choung, Myoung-Gun
Korean Journal of Medicinal Crop Science
/
v.14
no.2
/
pp.107-112
/
2006
Two Atractylodes species, A. japonica Koidz. ex Kitam. (AJ) and A. macrocephala Koidz (AM) were used in this study. AJ population had higher amounts of Sesquiterpenoids and stronger tolerance to root rot but less vigor of root growth than AM population. Two populations (AJ and AM) were crossed to make interspecific hybrid population. A total of 98 lines propagated clonally were selected from a cross of AJ and AM, and evaluated for contents of sesquiterpenoids, atractylon (ATLN) and atractylenolide III (AT3) using high performance liquid chromatography (HPLC), and growth characters such as plant height, stem number and root weight. HPLC profiles of the hybrids were compared with those of parent plants, and it demonstrated the production of introgression hybrid by crossing between AJ and AM. Of 98 clonal lines,10 lines were selected by 10% level based on the growth vigor and tolerance to root rot, and AJM2102-51 line showed the heaviest root weight (117.1 g/plant) among them. A total of 98 hybrid lines contained on average $0.16\;{\pm}\;0.10\;mg/g$ of $AT3,\;2.00\;{\pm}\;1.37\;mg/g$ of ATLN, and $2.16\;{\pm}\;1.40\;mg/g$ of total sesquiterpenoids, showing high coefficients of variation (above 65%). Ten lines having high contents of sesquiterpenoids were selected, and AJM2101-15 had the highest amount (9.83 mg/g) of ATLN, and showed 40.8 g/plant of root weight similar to mean value (39.9 g/plant) of hybrid lines. The result showed that the introgression of both characters of vigorous growth from AM and high sesquiterpenoids content from AJ could be possible to make new hybrid lines by crossing between AJ and AM.
Park, Jeong-Yong;Lee, Ji Yeon;Kim, Hyung Don;Jang, Gwi Yeong;Seo, Kyung Hye
Journal of Nutrition and Health
/
v.52
no.5
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pp.413-421
/
2019
Purpose: Astragalus membranaceus (AM) is an important traditional medicinal herb. Pharmacological research has indicated that AM has various physiological activities such as antioxidant, anti-inflammatory, immunoregulatory, anticancer, hypolipidemic, antihyperglycemic, and hepatoprotective activities. The bioactive substances responsible for the physiological activities in AM, including many antioxidant substances, change during the roasting process. This study investigated and compared the changes in the antioxidant constituents of AM caused by roasting. Methods: DPPH (1,1-diphenyl-2-picryl hydrazyl) and $ABTS^+$ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activities and their total phenolic content (TPC) were measured. High-performance liquid chromatography (HPLC) analysis was performed to confirm any changes in the isoflavonoids of roasted AM (R-AM),. The cell viability of UVB-induced HDF (Human dermal fibroblast) cells treated with AM and R-AM extracts was investigated. The comet assay was used to examine the inhibitory effects of R-AM extracts on DNA damage caused by oxidative stress. Results: The DPPH and $ABTS^+$ radical scavenging activities were $564.6{\pm}20.9$ and $108.2{\pm}3.1$ ($IC_{50}$ value) respectively, from the 2R-AM. The total phenol content was $47.80{\pm}1.40mg$ GAE/g from the 1R-AM. The values of calycosin and formononetin, which are the known isoflavonoid constituents of AM, were $778.58{\pm}2.72$ and $726.80{\pm}3.45{\mu}g/g$ respectively, from the 2R-AM. Treatment of the HDF cells with R-AM ($50{\sim}200{\mu}g/mL$) did not affect the cell viability. Furthermore, the R-AM extracts effectively protected against UVB-induced DNA damage. Conclusion: The findings of this study indicate that R-AM increases its isoflavonoid constituents and protects against UVB-induced DNA damage in HDF cells.
To investigate the thermal environment over the summertime asphalt pavements, an automatic weather observation system was installed at a parking lot paved with asphalt to observe various meteorological parameters and surface temperature from July 1 to September 30, 2014. Since the number of rainy days in summer of 2014 particularly after the mid July is more than that of average data, a ratio of daily peak surface temperature above $45^{\circ}C$ was 28% which was lower than the average. The observational data about hourly average surface temperature and various heat balance factors at days where daily peak surface temperature is above $45^{\circ}C$ are as follows: An hour that had the daily maximum temperature was around 15 pm and the value was $49^{\circ}C$ approximately. Net shortwave radiation was the highest at 12pm as $800W/m^2$ and much radiation of $500W/m^2$ was absorbed at the ground between 11am and 17pm. Sensible heat that was delivered from the ground to the atmosphere was evaluated as $200W/m^2$ between 10am and 19pm. underground transfer heat up to $100W/m^2$ was measured as negative from 19pm to the next day 8am, which indicated the lower atmosphere was heated at night.
Two experiments were conducted to investigate the effects of Animunin Powder${\circledR}$ supplementation on the growth perfonnance and blood components in nursery and growing pigs. In Exp. 1, ninety six pigs(initial mean live weights 27,6 ${\pm}$ 2.63kg) were randomly allocated into four treatments with six replications per each treatment. Four treatments were control(CON), control + 0.1% Antibiotics(AB), control + 0.1% Animunin Powder${\circledR}$(AM), and control + 0.1% Antibiotics + 0.1% Animunin Powder${\circledR}$(ABM). During 4 weeks experiment, ADG of pigs was significantly(p < 0.05) higher in ABM treatment compared with CON and AB treatments, Serum IgG concentration was higher(P < 0.05) in AM and ABM treatments than CON. Serum from treated pigs ABM had higher(P < 0.05) albumin concentration in comparison to CON and AB treatments. In addition, higher (p < 0.05) serum lymphocyte concentration was found in ABM treatment compared with CON and AM treatments. Digestibility of dry matter was higher(P < 0.05) in ABM treatment compared with AB and AM treatments. However, no statistical differences(P > 0.05) were found in total protein content of serum and crude protein digestibility of diets. In Exp. 2, seventy five pigs(initial mean live weights 40.22 ${\pm}$ 2.82kg) were randomly allocated into three treatments with 5 replicates per each treatment for 29 days. Three treatments were control(CON), control + 0.1% Animunin Powder${\circledR}$(AM1) and control + 0.2% Animunin Powder${\circledR}$(AM2). During the whole experiment, pigs fed AM1 and AM2 diets were shown higher ADG than pigs fed CON diet. The ADFI of AM1 diet is higher(P < 0.05) compared to that of CON diet. Serum IgG and albumin concentration of AM1 and AM2 treatments were greater than those of CON treatment(p < 0.05). Lymphocyte was highest for AM1 and lowest for CON, with intermediate for AM2(P < 0.05). In conclusion, Animunin Powder${\circledR}$ was effective for improving growth performance and to increase the concentrations of blood IgG, albumin, total protein and lymphocyte in nursery and growing pigs.
Purpose: Human body have biological rhythmic pattern in a day, which is affected by internal and external environmental factors. We investigated whether respiratory function was fluctuated according to the influence of time-of-day (around at 9 am, 1 pm, and 6 pm) in health subjects, using pulmonary function test (PFT). Methods: Eighteen healthy volunteers (8 men, mean ages; $22.4{\pm}1.6$, mean heights; $166.61{\pm}9.60$, mean weight; $59.3{\pm}10.3$) were recruited. Pulmonary function test (PFT) was measured at three time points in day, around 9 am, 1 pm, and 6 pm in calm research room with condition of under 55dB noise level, using a spirometer (Vmax 229, SensorMecis, USA). Forced vital capacity (FVC), forced expiratory volume at one second (FEV1), FVC/FEV1, and peak expiratory flow (PEF) were acquired. Results: In comparison of raw value of PFT among three time points, subjects showed generally better respiratory function at 9 am, than at other points, although no significance was found. In comparison of distribution of ranking for respiratory function in each individual, only PEF showed significant difference. In general, distributional ratio of subjects who showed best performance of respiratory function in a day was high. Conclusion: These findings showed that circadian rhythm by diurnal pattern was not detected on respiratory function throughout all day. But, best performance on respiratory function was observed mostly in the morning, although statistical significance did not exist.
Journal of electromagnetic engineering and science
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v.8
no.1
/
pp.17-22
/
2008
It is presented that sampling rates for behavioral modeling of quasi-memory less nonlinear devices can be far less than the Nyquist rate of the input signal. Although it has been believed that the sampling rate of nonlinear device modeling should be at least the Nyquist rate of the output signal, this paper suggests that far less than the Nyquist rate of the input signal can be applied to the modeling of quasi-memoryless nonlinear devices, such as frequency multipliers. To verify, a QPSK signal at 820 MHz were applied to a frequency tripler, whereby the device can be utilized as an up-converting mixer into 2.46 GHz with the aid of digital predistortion. AM-AM, AM-PM and PM-PM can be successfully measured regardless of sampling rates.
Park, Sang-Chul;Sung, A-Young;Kim, Tae-Hyun;Kim, Douk-Hoon
Journal of Korean Ophthalmic Optics Society
/
v.10
no.1
/
pp.35-40
/
2005
Subjective and objective visions were measured on young adults(mean 21 yrs, 126 eyes) who were free of any ocular diseases and laser surgery and none wore contact lenses. The aim of this study was to investigate the diurnal variation of vision through subjective and objective measurements. Subjective visual acuity were measured at 5 m three times a day, morning(8:00 AM-10:00 AM), noon(12:00 PM-2:00 PM) and afternoon(4:00 PM-6:00 PM). The instrument used for objective refraction right after visual acuity measurement was Nvision-K 5001(shin-nippon) which unique in being able to disregard subject's accommodation because of its unrestricted viewing conditions. Also, we measured that three times and then calculated the average values. The result showed that an average subjective visual acuity in the morning, noon, afternoon were 0.256(${\pm}0.263$), 0.266(${\pm}0.276$), 0.242(${\pm}0.249$) respectively. Average spherical equivalent power in objective refraction of right eyes showed -3.416 D(${\pm}2.907$), -3.359 D(${\pm}2.735$), -3.297 D(${\pm}2.709$) respectively and dioptric power was decreased from morning to afternoon. Vision changed throughout the day in both subjective and objective measurements nevertheless its variations were statistically insignificant(p<0.05). Therefore it does not seem to matter of time for either visual acuity test or refraction.
The Journal of Korean Institute of Electromagnetic Engineering and Science
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v.10
no.5
/
pp.711-718
/
1999
Some of modern communication systems make use of broadband digital modulation with 5 MHz and 10 MHz. In the broadband communication systems, the nonlinear properties of power amplifier have a great effect on all systems. This paper analyzed the ACPR characteristics of WLL reverse link power amplifier with the varying AM and PM nonlinearities and suggests the required backoff from 1 dB compression point to satisfy the ACPR specifications. Also the comparions between the measured and the simulated characteristics are presrnted.
Journal of the Korean Association of Oral and Maxillofacial Surgeons
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v.32
no.3
/
pp.189-199
/
2006
Purpose of study: Partial thickness skin graft is the golden standard regimen for full-thickness skin defect caused by burn or trauma. However, in case of extensive burns of more than 50% of total body surface area, the donor site is not sufficient to cover all defects. As a second choice, allograft, xenograft and synthetic materials have been used to treat skin defect. Among them the amniotic membrane(AM) was used as a biological dressing for centuries because of its potential for wound healing. In this study, quantification of EGF in AM and effect of AM-collagen complex on full thickness skin defects was examined. Materials & Methods: The concentration of EGF in fresh, deep frozen and freeze-dried AM was evaluated by ELISA. EGF-R immunostaining was performed in freeze-dried AM. SD rats weighing 250${\sim}$300g was used for wound healing experiment. Three full thickness skin defects(28mm diameter) were made on dorsal surface of SD rat. The control group was covered by Vaselin gauze and AM-collagen complex and $Terudermis^{(R)}$. was grafted in two other defects. Healing area, Cinamon's score were evaluated before biopsy. Grafted sites were retrieved at 3 days, 1 week, 2 weeks and 4 weeks after operation. H & E and Factor VIII immunohistochemical stain was performed to evaluate the microscopic adhesion and structural integrity and microvessel formation. Results: 1. EGF concentration of fresh, deep frozen and freeze-dried AM showed similar level and EGF-R was stained in epithelial layer of freeze-dried AM. 2. At 4 weeks after grafting, the healing area of AM-collagen and Terudermis group was 99.29${\pm}$0.71% and 99.19${\pm}$0.77 of original size. However, that of control group was 24.88${\pm}$2.90. 3. The Cinamon's score of AM-Collagen and $Terudermis^{(R)}$. group at 4 weeks was 15.6${\pm}$1.26 and 14.6${\pm}$3.13 and that of control group was 3.7${\pm}$0.95. Significant difference was observed among control and experimental groups(p<0.05). 4. Histologic examination revealed that AM protected leukocyte infiltration and epithelial migration was nearly completed at 4 weeks. $Terudermis^{(R)}$. group showed mild neutrophil infiltration until 2 weeks and completion of epithelization at 4 weeks. Control group showed massive leukocyte infiltration until 4 weeks. 5. Microvessels were increased sharply at 1 week and control group at 1 and 4 week showed significant differences with $Terudermis^{(R)}$. group of same interval(p<0.05) but no differences were found with AM group(p<0.05). Conclusion: EGF and EGF-R were well preserved in freeze-dried AM. AM attached to collagen acted as excellent biologic dressing which had similar effect with $Terudermis^{(R)}$. AM showed anti-inflammatory action and healing was completed at 4 weeks after full-thickness skin defect.
Kim, Dong-Soon;Paik, Sang-Hoon;Shim, Tae-Sun;Lim, Chae-Man;Lee, Sang-Do;Koh, Youn-Suck;Kim, Woo-Sung;Kim, Won-Dong
Tuberculosis and Respiratory Diseases
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v.45
no.1
/
pp.116-127
/
1998
Background: The natural course of sarcoidosis is variable from spontaneous remission to significant morbidity or death. So the assessment of disease activity is important but no single parameter was generally accepted as a good marker. Recently several studies suggested that adhesion molecules, especially ICAM-1 can be a marker, but there are some controversies. And only few data are available about the relationship of ICAM-1 with clinical follow-up course. Methods: We measured the expression of adhesion molecules on BAL cells by flow cytometry and the level of soluble ICAM-1(sICAM-1) in serum and BALF at the time of diagnosis in 12 patients with active disease and 7 inactive sarcoidosis(5 male, 14 female, mean age: $39.4{\pm}10.7$ years, mean follow-up : $20{\pm}15$ months). Follow-up clinical course were compared with the changes in serum sICAMA-1 level and the adhesion molecule on BAL cells. Results: In the patients with active disease, the ICAM-1 on AM(RMFI: $3.68{\pm}1.71$) and sICAM-1 level in serum($582{\pm}193$ng/ml) and BAL fluid($47.8{\pm}16.5$ng/ml) were all higher than those of 7 inactive disease(RMFI: $1.89{\pm}0.75$, p=0.0298, serum: $294{\pm}117$ ng/ml, p=0.0049, BALF: $20.9{\pm}8.3$ ng/ml). In the active sarcoidosis, ICAM-1 on AM(RMFI : $1.51{\pm}0.84$) and serum sICAM-1 were decreased after the therapy($250{\pm}147$ ng/ml) but no significant change was noted in inactive disease. Also we found the initial ICAM-1 on AM and serum sICAM-1 had a significant correlation with the degree of improvement in PFT after the therapy. During the follow-up, the disease relapsed in 4 patients after the discontinuation of steroid and the serum sICAM-1 level went-up again at the time of relapse. Conclusion: Our data suggest that the serum sICAM-1 level and the ICAM-1 expression on AM can be a good marker of disease activity and also a predictor of outcome in sarcoidosis.
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