• Journal of Microbiology and Biotechnology
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• v.4 no.2
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• pp.102-107
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• 1994

The $\beta$-1,4-endoglucanase gene from Actinomyces sp. 40 was cloned into Escherichia coli DH5$\alpha$ with pUC19. Chromosomal DNA from Actinomyces sp. 40 was cleaved with the restriction enzyme Sau3AI and ligated into pUC19 for the transformation of Escherichia coli DH5$\alpha$. Positive clones of $\beta$-1,4-endoglucanase gene were detected as the clear zones on a medium supplemented with carboxymethylcellulose (CMC). This transformant possessed a single plasmid, designated pDS1, which contained the vector DNA and a 3.5 kilobase (kb) Sau3AI insertion fragment encoding endoglucanase. The size of the cloned fragment was reduced to 2.0 kb. The endoglucanase activity produced by the E. coli DH5$\alpha$ (pDS6) was higher than that of Actinomyces sp. 40 strain. The optimum pH and temperature of the cloned enzyme were pH 4.0$\sim$5.0 and 55$^{\circ}C$, respectively. The cloned enzyme was stable at 55$^{\circ}C$ or below and in buffer ranging from pH 4.0 to 7.0. The enzyme degraded CMC but did not degrade xylan, cellobiose, and methyl-umbelliferylcellobiopyranoside (MUC).

• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.129-133
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• 1993

The 44Md plasmid of Bacillus thruingiensis var. kurstaki HD-1(B. t k HD-1) was partially digested with Sau3AI and the fragments were cloned into E. coli HB101 on vector pBR322. Of 2, 950 clones with a recombinant pBR322, only one clone KC1 was determined to have the gene for crystal toxic proteins from the 44Md plasmid of B. t k HD-1 at the BamHI site of pBR322. The recombinant pBR322 was named pKC1 and its molecular size was 12kb. The KC1 produced a protein which was toxic to the silkworm and antigenically similar to the crystal toxic protein of B. t k HD-1. Also, electrophoretic mobility of the KC1 protein was apparently the same as that of the crystal toxic protein of B. t k HD-1.

• Journal of Korea Entertainment Industry Association
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• v.15 no.1
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• pp.153-160
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• 2021

In recent universities, the new-normal era. There is a growing interest in nurturing creative thinking learners who have converged thinking necessary for the AI era based on big data. This study intends to propose the expansion of science liberal arts education necessary to meet the education needs of these universities. To this end, first the status of convergence education in universities and the elements necessary for the learners' creative ideas were examined. Second, learners who took a convergence lecture at a university will look at the development case of in which convergence thinking, creative thinking, and gaming are implemented. Based on this, we would like to propose an expanded organization and operation of science liberal arts education as a way to enhance the creative thinking of learners and their competencies through convergence classes at universities.

• Convergence Security Journal
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• v.24 no.2
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• pp.31-38
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• 2024

Recent expansion in cloud service usage has heightened the importance of cloud security. The purpose of this study is to analyze current research trends in the field of cloud security and to derive implications. To this end, R&D project data provided by the National Science and Technology Knowledge Information Service (NTIS) from 2010 to 2023 was utilized to analyze trends in cloud security research. Fifteen core topics in cloud security research were identified using LDA topic modeling and ARIMA time series analysis. Key areas identified in the research include AI-powered security technologies, privacy and data security, and solving security issues in IoT environments. This highlights the need for research to address security threats that may arise due to the proliferation of cloud technologies and the digital transformation of infrastructure. Based on the derived topics, the field of cloud security was divided into four categories to define a technology reference model, which was improved through expert interviews. This study is expected to guide the future direction of cloud security development and provide important guidelines for future research and investment in academia and industry.

• Microbiology and Biotechnology Letters
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• v.14 no.3
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• pp.245-250
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• 1986

Antibiotic resistant bacteria isolated from the river water in Cheongju City were studied on the transfer of their R-plasmids in water environment. The R-plasmids were transferred by conjugation between the isolates ai the frequencies of 1.1$\times$10$^{-6}$ to 1.2$\times$10$^{-7}$ under laboratory condition and 1.2$\times$10$^{-7}$ to 1.0$\times$10$^{-9}$ in natural environment. The R-plasmids isolated from those bacteria were also transferred into the recipient cells of E. coli HB101 at the frequencies of 1.7-6.7$\times$10$^{-6}$. The AP$^{r}$Cm$^{r}$Tc$^{r}$-plasmid of isolate T-44 which were transformation by conjugation and transformation was determined to be 9.01 kilobses in molecular size. When the AP$^{r}$Cm$^{r}$Tc$^{r}$-plasmid DNA was treated with restriction endonuclease, the plasmid appeared to have one restriction site for EroRI and BamHI, respectively, and three sites for Pst I endonuclease.

• Journal of Korean Elementary Science Education
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• v.43 no.1
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• pp.148-157
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• 2024

The purpose of this study was to develop an artificial intelligence-based marine ecological education program to improve the ecological sensitivity of elementary school students. The program was taught 11 times within 4 weeks, and an ecological sensitivity test was conducted before and after the program. The statistical results of the tests showed that the developed program improved the ecological sensitivity of elementary school students. Through in-depth interviews, improvements were found in all the areas, such as empathy for the living things, interest in nature, enjoyment of nature, and wonder about nature. Through the marine ecological classes, which used artificial intelligence and virtual reality, the students were able to get closer to nature, and the student participation activities showed a positive effect on their ecological sensitivity. This indicates that experience-oriented education methods are more effective than simple explanatory classes to improve the students' ecological sensitivity, and artificial intelligence technology proved effective in increasing the students' immersion in the class.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.24 no.3
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• pp.123-131
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• 2024

The dispute between SK and Netflix, which has been going on for more than three years, ended in the direction of dropping the lawsuit and establishing a cooperative relationship between the two companies. However, as Internet traffic usage is likely to increase further in the future due to digital transformation and activation of generated AI, conflicts between domestic mobile carriers and global CP operators over network usage fees can arise at any time. In this study, the issues of the dispute related to network usage fees that occurred between SK and Netflix were examined, and different implications were drawn for each issue. The cost and scope of network usage considerations are an issue that must be determined entirely by negotiations between operators. However, if a dispute occurs between operators, user damage such as speed delays may occur, so it is necessary to prepare a policy alternative. As the domestic media industry has grown cooperatively with global CPs, it is considered important to form a reciprocal relationship between domestic mobile telecommunication operators and global CP operators regarding network usage fees in the future.

• Journal of Christian Education in Korea
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• v.66
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• pp.413-437
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• 2021

Humans are created as spiritual beings that can relate to God. However, when a human spirit refuses to transform through confronting God, it experiences a crisis. A spiritual crisis results from disconnecting with God, who is the ultimate foundation, but we humans try to overcome such absence through accomplishments and efforts. In this technological age, the ethics issues of AI (Artificial Intelligence), robots, and cloning are related to anthropology. The development of the mind, heart, and logic cannot suggest a basis for destruction and confusion as much as the development of the world. In fact, education focused on the human mind cannot be considered holistic. Mind, together with thought, will, and belief, plays a crucial role in making choices and leading a human life. So it is actively studied in other domains other than Christian education. However, although the human spirit takes care of some territory of humanity, unlike the mind, it can neither be partial nor fragmentary. Instead, it manages the transformation that influences the core of human life. Therefore, Christian education must clearly concentrate on the spirit rather than on other human elements, intentionally concerning spiritual transformation through encounters with God. In other words, Christian education is the passage connecting a human spirit to God's presence at work, which enables us to understand the human being as a whole. For this, we must put our efforts to increase the chances of encountering God through Christian education. While "Encounter" requires both parties' interaction, "Transformation" stresses God as the main agent and His proactive nature. I also want to emphasize "worship" as the opportunity to communicate and experience God in our daily lives. By examining the preparation and the process of the spiritual transformation of humans, this paper would offer a theological foundation for continued transformation of the human spirit in the faith community, rather than personal experience or conviction.

• Journal of the Korean Home Economics Association
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• v.49 no.1
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• pp.79-91
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• 2011

The purposes of this study were firstly to explore the practical implications that of 'low carbon and green growth' policy, which is at the top of the Government's agenda provides to family, and secondly to propose some visions for a future based on those implications. The results of this study were as follows: Firstly, in terms of a global perspective, there is now a worldwide trend towards the adoption of 'low carbon and green growth' policies. Secondly, the Government-driven 'green growth policy' demands a total transformation, that is, revolution, not only in terms of our industries, but also in terms of our mentality and ordinary life. Thirdly, the driving force for this life revolution lies in having green life style, and the family is the primary agent for making the green life style a practical reality.

• The Korean Journal of Applied Statistics
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• v.14 no.1
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• pp.25-37
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• 2001

The marginal model is proposed for the analysis of data which have c(2: 3) categories in the 3 x 3 cross-over trials with three periods and three treatments. This model could be used for the counterpart of the Kenward-Jones' joint probability one and should be the generalization of Balagtas et ai's univariate marginal logits one, which analyze the treatment effects in the 3 x 3 cross-over trials with binary response variables[Kenward and Jones(1991), Balagtas et al(1995)]. The model equations for the marginal probability are constructed by the three types of link functions. The methods would be given for making of the link function matrices and model ones, and the estimation of parameters shall be discussed. The proposed model is applied to the analysis of Kenward and Jones' data.