• 생물정신의학
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• 제22권4호
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• pp.179-186
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• 2015

Objectives Adrenergic alpha 1 and 2 receptors work as pathways to control the serotonergic neuron moderation and mirtazapine acts as antagonist of these receptors. The adrenoreceptor alpha 1a (ADRA1A) gene, which encodes adrenergic alpha 1 receptor, has Arg-347Cys genetic polymorphism and the polymorphism has strong relationship with many neuro-psychiatric diseases. In this study, we explored the relationship between ADRA1A R347C polymorphism and mirtazapine treatment response in Koreans with major depression. Methods 352 patients enrolled in this study, and the symptoms were evaluated by 17-item Hamilton Depression Rating (HAMD-17) scale. After 1, 2, 4, 8, and 12 weeks of mirtazapine treatment, the association between ADRA1A R347C polymorphism and remission/response outcomes was evaluated. Results Treatment response to mirtazapine was significantly better in T allele carriers than C allele homozygotes after 12 weeks of mirtazapine monotherapy. The percentile decline of HAMD-17 score in T allele carriers was larger than that of C allele homozygotes. ADRA1A R347C genotypes were not significantly associated with remission. Conclusions The result showed that treatment response to mirtazapine was significantly associated with ADRA1A R347C genetic polymorphism. T allele carriers showed better treatment response than C allele homozygotes. It can be supposed that T allele carriers have a trend of better treatment response to mirtazapine monotherapy.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1256-1264
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• 2016

Adrenergic, alpha-1B-, receptor (ADRA1B) and peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B) genes are involved in regulation of hen ovarian development. In this study, these two genes were investigated as possible molecular markers associated with hen-housed egg production, egg weight (EW) and body weight in Chinese Dagu hens. Samples were analyzed using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique, followed by sequencing analysis. Two novel single nucleotide polymorphisms (SNPs) were identified within the candidate genes. Among them, an A/G transition at base position 1915 in exon 2 of ADRA1B gene and a T/C mutation at base position 6146 in the 3'- untranslated region (UTR) of PPARGC1B gene were found to be polymorphic and named SNP A1915G and T6146C, respectively. The SNP A1915G (ADRA1B) leads to a non-synonymous substitution (aspartic acid 489-to-glycine). The 360 birds from the Dagu population were divided into genotypes AA and AG, allele A was found to be present at a higher frequency. Furthermore, the AG genotype correlated with significantly higher hen-housed egg production (HHEP) at 30, 43, 57, and 66 wks of age and with a higher EW at 30 and 43 wks (p<0.05). For the SNP T6146C (PPARGC1B), the hens were typed into TT and TC genotypes, with the T allele shown to be dominant. The TC genotype was also markedly correlated with higher HHEP at 57 and 66 wks of age and EW at 30 and 43 wks (p<0.05). Moreover, four haplotypes were reconstructed based on these two SNPs, with the AGTC haplotype found to be associated with the highest HHEP at 30 to 66 wks of age and with higher EW at 30 and 43 wks (p<0.05). Collectively, the two SNPs identified in this study might be used as potential genetic molecular markers favorable in the improvement of egg productivity in chicken breeding.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제24권1호
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• pp.21-27
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• 2013

Objectives : Dysregulation of the central noradrenergic system may be involved in the pathophysiology of attention-deficit hyperactivity disorder (ADHD). The aim of this study was to examine the differences in pre- and post-treatment cerebral perfusion according to the MspI polymorphisms of the alpha-2A-adrenergic receptor gene (ADRA2A) in children with ADHD. Methods : Thirty seven drug-naive ADHD children (8.9+1.8 years old, M=32, F=5) were genotyped. Baseline single-photon emission computed tomography (SPECT) and clinical assessments were performed for ADHD children. After treatment with methylphenidate for eight weeks, SPECT and clinical assessment were repeated. Results : No differences in baseline clinical assessments or cerebral perfusion were observed according to the MspI genotype. However, after treatment, ADHD children with the G/G genotype at the MspI polymorphism showed hyperperfusion in the right cerebellar declive (p=.001, uncorrected) and hypoperfusion in the left lentiform nucleus and left cingulate gyrus (p<.001 and p=.001, uncorrected), compared to children without the G/G genotype. Conclusion : Although the results of this study should be interpreted cautiously, they suggest a possible role of the MspI polymorphisms of the ADRA2A gene in methylphenidate-induced changes in cerebral perfusion.

• 한국자원식물학회지
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• 제10권2호
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• pp.151-158
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• 1997

본 연구는 콩계통들의 지방함량이 서로 다른 가상요인에 어떻게 영향을 받는 지를 규명하기 위해 미국 노스캐롤라이나지방의 5개 지역에서 3년에 걸쳐 수행하였다. 각 계통의 지방함량 및 지방수량들이 기상요인에 대한 Linear response가 조사되었고, 또한 최저기상 반응모델을 결정하기 위해 Stepwise Selection Program이 사용되었다. 콩지방함량, 지방수량은 기상 요인인 온도와 강우량에 의해 크게 영향을 받았으며 성숙시기에 온도가 가장 높고, 최고최저 온도범위가 가장 작은 환경에서 지방함량이 가장 낮게 나타났고, 최저온도 변이가 가장 작고 강우량변이가 가장 큰 환경에서 지방수량이 가장 높게 나타났다. 계통들의 대부준으 지방함량이 MxDT, HTD, ADT, ADTRg에 반비례하는 경향을 나타내었으며 이는 성숙시기에 온도가 높아질수록 지방함량이 감소한다는 것을 의미한다. 그러나 NC107은 MxDT, ADT에 비례하는 경향을 보였다. 모든 계통들이 VMnDT, VADTRg, ADRa에 비례하는 경향을 나타내었고 이는 최저온도와 최고최저 온도범위의 변이가 클수록, 평균강우량이 많을수록 지방함량은 증가한다는 것을 의미한다. 11계통이 1개 내지 3개의 기상변수를 가지는 최적기상모델을 형성하였다. 그러나 NC109와 NC105는 모든 기상변수에 유의한 Linear 반응을 나타내지는 않았으나 전자는 2개의 기상변수를 가지는 최적기상모델을 형성하였고 후자는 가지지 않았다. 이는 NC109는 지방 함량이 아마도 기상변수 하나에 의한 영향보다도 2개 변수의 상호작용에 의한 영향이 큰 것으로 추측 할 수 있고, NC105는 지방함량이 기상환경에 상대적으로 더 안정하기 때문인 것으로 생각된다. 위와 같은 사실은 콩지방함량의 기상반응에 대한 품종적 차이가 상당히 크다는 것을 의미한다. 지방수량은 ADTRg와 VADRa에 반비례하는 경향을 나타내었으며 이는 최고최저 온도차이와 평균강수량 변이가 클수록 지방수량은 감소한다는 것을 의미한다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.112-112
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• 2002

Under normal conditions, women produce a single dominant follicle that participates in a single ovuation each menstrual cycle. But Polycystic ovary syndrome(PCOS) conditions, folliculogenesis does not proceed normally. This condition leads to the accumlation of large numbers of small graffian follicles in which the theca interstitial cells (TIC) produce abnormally large amounts of androgen. PCOS is probably the most common endocrine disorder, affecting women of reprodutive age with 5-10% prevalence estimate. Chronic anovulation, hyperandrogenism, hirsutism, obesity, infertility and polycystic ovaries are clinical hallmarks of women with PCOS. Its etiology remains unknown. To investigate the gene expression pattern of ovary in PCO-induced rat, we used cDNA expression analysis. Total RNA was extracted from the ovary of PCO-induced rat and reverse-transcribed in the presence of［$\alpha$$^{32}$P］-dATP Which were hybridized to Atlas$^{TM}$ Rat Toxicology 1.2 array (Clontech) representing approximately 1176 rat genes. We compared gene expression between ovary of pco-induced immature female rats and control. Differential gene expression profiles were revealed (LIFR-alpha, ADRA1A, Heat shock 90-kDa protein A, PDGFRA). Reverse transcription-polymerase chain reaction(RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the altered expression of genes and PCO is a matter of further investigation. This study was supported by Korea Science and Engineering Foundation(KOSEF)

• 한국작물학회지
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• 제42권5호
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• pp.539-547
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• 1997

This study was carried out to identify how soybean seed protein concentration is influenced by climatic factors. Twelve lines selected for seed protein concentration were studied in 13 environments of North Carolina. Sensitivity of seed protein concentration, total seed protein, and seed yield to climatic variables was investigated using a linear regression model. Best response models were determined using two stepwise selection methods, Maximum R-square and Stepwise Selection. There were wide climatic effects in seed protein concentration, total protein and seed yield. The highest protein concentration environment was characterized by the most high temperature days(HTD) and the smallest variance of average daily temperature range (VADTRg), while the lowest protein concentration environment was distinguished by the fewest HTD and the largest VADTRg. For protein concentration, all lines responded positively to average maximum daily temperature(MxDT), HTD, and average daily temperature range(ADTRg) and negatively to ADRa, while they responded positively or negatively to average daily temperature(ADT), variance of average minimum daily temperature (VMnDT), and VADTRg, indicating that genotypes may greatly differ in degrees of sensitivity to each climatic variable. Eleven lines seemed to have best response models with 2 or 3 variables. Exceptionally, NC106 did not show a significant sensitivity to any climatic variable and thus did not have a best response model. This indicates that it may be considered phenotypically more stable. For total seed protein and seed yield, all the lines responded negatively to both ADTRg and VADRa, suggesting that synthesis of seed components may increase with less daily temperature range and less variation in daily rainfall.