• Molecules and Cells
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• 제44권9호
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• pp.637-646
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• 2021

Free fatty acids are converted to acyl-CoA by long-chain acyl-CoA synthetases (ACSLs) before entering into metabolic pathways for lipid biosynthesis or degradation. ACSL family members have highly conserved amino acid sequences except for their N-terminal regions. Several reports have shown that ACSL1, among the ACSLs, is located in mitochondria and mainly leads fatty acids to the β-oxidation pathway in various cell types. In this study, we investigated how ACSL1 was localized in mitochondria and whether ACSL1 overexpression affected fatty acid oxidation (FAO) rates in C2C12 myotubes. We generated an ACSL1 mutant in which the N-terminal 100 amino acids were deleted and compared its localization and function with those of the ACSL1 wild type. We found that ACSL1 adjoined the outer membrane of mitochondria through interaction of its N-terminal region with carnitine palmitoyltransferase-1b (CPT1b) in C2C12 myotubes. In addition, overexpressed ACSL1, but not the ACSL1 mutant, increased FAO, and ameliorated palmitate-induced insulin resistance in C2C12 myotubes. These results suggested that targeting of ACSL1 to mitochondria is essential in increasing FAO in myotubes, which can reduce insulin resistance in obesity and related metabolic disorders.

• Journal of Nutrition and Health
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• 제55권1호
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• pp.70-84
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• 2022

Purpose: Skeletal muscles display significant heterogeneity in metabolic responses, owing to the composition of metabolically distinct fiber types. Recently, numerous studies have reported that in skeletal muscles, suppression of genes related to fatty acid channeling alters the triacylglycerol (TAG) synthesis and switches the energy substrates. However, such responses may differ, depending on the type of muscle fiber. Hence, we conducted in vitro and animal studies to compare the metabolic responses of different types of skeletal muscle fibers to the deficiency of fatty acyl-CoA synthetase (Acsl)6, one of the main fatty acid-activating enzymes. Methods: Differentiated skeletal myotubes were transfected with selected Acsl6 short interfering RNA (siRNA), and C57BL/6J mice were subjected to siRNA to induce Acsl6 deficiency. TAG accumulation and expression levels of insulin signaling proteins in response to acute glucose supplementation were measured in immortalized cell-based skeletal myotubes, oxidative muscles (OM), and glycolytic muscles (GM) derived from the animals. Results: Under conditions of high glucose supplementation, suppression of the Acsl6 gene resulted in decreased TAG and glycogen synthesis in the C2C12 skeletal myotubes. The expression of Glut4, a glucose transporter, was similarly downregulated. In the animal study, the level of TAG accumulation in OM was higher than levels determined in GM. However, a similar decrease in TAG accumulation was obtained in the two muscle types in response to Acsl6 suppression. Moreover, Acsl6 suppression enhanced the phosphorylation of insulin signaling proteins (Foxo-1, mTORc-1) only in GM, while no such changes were observed in OM. In addition, the induction ratio of phosphorylated proteins in response to glucose or Acsl6 suppression was significantly higher in GM than in OM. Conclusion: The results of this study demonstrate that Acsl6 differentially regulates the energy metabolism of skeletal muscles in response to glucose supplementation, thereby indicating that the fiber type or fiber composition of mixed muscles may skew the results of metabolic studies.

• 한국산업융합학회 논문집
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• 제6권2호
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• pp.141-147
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• 2003

In this paper, the vibration characteristics of a linear stepping motor(LSM) are analyzed using the finite element method(FEM : Flux2D) and ACSL. A magnetic equivalent circuit is based on the structure of the LSM, and then the electric equivalent circuit of the LSM is derived by solving equations for the magnetic equivalent circuit. A normal force is calculated using FEM. And the vibration characteristics of the LSM are simulated by the ACSL with the voltage equations, the thrust equations, the normal force equations and the kinetic equations, and are measured by LASER experimental system.

• 조명전기설비학회논문지
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• 제18권1호
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• pp.122-128
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• 2004

본 논문에서는 가동자의 이동방향에 따른 진동과 고정자에 대하여 수직으로 작용하는 진동을 동시에 고려할 수 있는 이론을 제시함에 있어서, LSM의 구조를 기초로 자기등가회로를 구성하였으며, 자기등가회로에 대한 방정식을 이용하여 LSM의 전기적인 등가회로를 유도하였다. 그리고, LSM의 변위에 따른 수직력 분포를 유한요소법(Flux2D)으로 계산하였으며 이를 방정식으로 나타내었다. 최종적으로 1상 여자방식에 의한 가동자의 이동방향에 따른 진동과 고정자에 대하여 수직으로 작용하는 진동을 전압방정식, 추력 및 수직력 방정식, 운동방정식을 사용하여 ACSL(Advanced Continuous Simulation Language)에 의하여 시뮬레이션 하였다.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.215-219
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• 2011

Acyl-CoA synthetase 4(ACSL4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues and postulated to modulate eicosanoid production. The human and mouse ACSL4 gene are mapped on chromosome X. The female mice heterozygous for ACSL4 deficiency became pregnant less frequent1y and produced small litters, with 40% of embryos surviving gestation. In this study, we examined the regulation of ACS4 by estradiol-$17{\beta}$ and progesterone (P4) in the human endometrial cancer cell line HTB-1B. ACSL4 mRNA was increased in a dose-dependent manner. Also, expression of ACSL4 gene was up-regulated in a time-dependent manner in HTB-1B cells. However, combined treatment with progesterone and estradiol-$17{\beta}$ modestly decreased the levels of ACS4L mRNA as compared with the estradiol-$17{\beta}$ and progesterone respectively. Overall, these results suggest that the ACSL4 gene is regulated by progesterone and estradiol-$17{\beta}$ in the HTB-1B cells.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2001년도 전력전자학술대회 논문집
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• pp.14-17
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• 2001

In this paper, the vibration characteristics of a 2-phase Hybrid type Linear Stepping Motor(HLSM) are analyzed using the ACSL. A magnetic equivalent circuit is based on the structure of the HLSM, and then the electric equivalent circuit of the HLSM is derived by solving equations for the magnetic equivalent circuit. A normal force is calculated using FEM(Flux2D). And the vibration characteristics of the HLSM are simulated by the ACSL with the voltage equations, the thrust equation, the normal force equation and the kinetic equation.

• 전력전자학회논문지
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• 제4권6호
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• pp.554-560
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• 1999

리니어 스텝핑 모터(Linear Stepping Motor : 이하 LSM이라 칭함)는 운동 변환기구 없이 입력신호에 따라 선형적인 변위를 발생시키는 다이렉트 드라이브(Direct Drive)방식의 전동기이다. 따라서 LSM은 자동반송장치, 의료기기, OA,FA 응용 등에 다양하게 사용되고 있다. 그러나 LSM은 구동시 구조적으로 큰 진동이 발생됨으로서 추력이 급격히 떨어질 때가 있으며, 최악의 경우에는 역전이나 탈조현상이 발생하고, 또한 소음의 원인이 되기 때문에 응용에 있어서 많은 제약을 받고 있는 실정이다. 여기에 대응하기 위해, 본 논문에서는 권선 인덕턴스에 저장된 에너지와 LSM의 유도전압을 이용한 새로운 1상 여자방식에 의한 진동억제 방법을 보여주며, 또한 LSM의 구조를 기초로 자기등가회로를 구성하였으며, 또한 자기등가회로에 대한 방정식을 이용하여 LSM의 전기적인 등기회로를 유도하였다. 그리고, LSM의 새로운 1상 여자방식에 의한 진동특성을 전압방정식과 추력방정식 및 운동방정식을 사용하여 ACSL에 의하여 시뮬레이션 하였으며, 레이저 실측 시스템에 의한 실측결과와 비교·검토하였다.

• 전력전자학회논문지
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• 제8권2호
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• pp.199-210
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• 2003

본 논문에서는 계통연계형 태양광발전시스템의 고립운전방지를 위한 새로운 고리운전 검출기법을 연구한다. 이를 위하여 계통연계형 태양광발전시스템 및 고립운전시의 특성에 대하여 기술하였다. 또한 고립운전의 검출특성을 향상시키기 위한 새로운 검출기법을 제시하고 해석하였으며 검출시험을 위한 부하조건 선정을 위한 부하임피던스 곡선을 해석적으로 유포하였다 ACSL 시뮬레이션 및 실험을 통하여 제시한 고립운전 검출기법 및 해석결과를 검증하였다.

• 한국산학기술학회논문지
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• 제4권4호
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• pp.323-328
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• 2003

본 논문에서는 통신 기지국용 전원으로 사용할 수 있는 직렬 공진형 컨버터 시스템의 새로운 제어방식을 제안하였다. 주파수 제어 직렬 공진형 컨버터 시스템은 병렬 출력 직렬 공진(Parallel Output Series Resonant: POSR)형태이기 때문에 부하변동에 강인하다. 또한 스위칭 주파수를 제어함으로써 입력전압의 변화에도 안정된 출력을 제공한다. 실제 시스템 제작에 앞서 제안된 병렬 출력 직렬 공진형 컨버터 시스템의 분석을 하였고 ACSL(Advanced Continuous Simulation Language)을 이용한 모의실험을 하였다. 또한 실제의 시스템을 제작 및 실험을 통하여 시스템 특성을 확인하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 B
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• pp.852-854
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• 2001

In this paper, the vibration characteristics of a 2-phase Hybrid type Linear Stepping Motor(HLSM) are analyzed using the ACSL. A magnetic equivalent circuit is based on the structure of the HLSM, and then the electric equivalent circuit of the HLSM is derived by solving equations for the magnetic equivalent circuit. A normal force is calculated using FEM(Flux2D). And the vibration characteristics(Continuous vibration) of the HLSM are simulated by the ACSL with the voltage equations, the thrust equation, the normal force equation and the kinetic equation.