Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Regulation of Long-Chain Acyl-CoA Synthetase 4 Expression with Progesterone and Estradiol-$17{\beta}$

프로게스테론과 에스트라디올 $17{\beta}$에 의한 Long-Chain Acyl-CoA Synthetase 4의 발현 조절

  • Park, Hyo-Young (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Kang, Man-Jong (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
  • 박효영 (전남대학교 농업생명과학대학 동물자원학부) ;
  • 강만종 (전남대학교 농업생명과학대학 동물자원학부)
  • Received : 2011.08.26
  • Accepted : 2011.09.02
  • Published : 2011.09.30
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Abstract

Acyl-CoA synthetase 4(ACSL4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues and postulated to modulate eicosanoid production. The human and mouse ACSL4 gene are mapped on chromosome X. The female mice heterozygous for ACSL4 deficiency became pregnant less frequent1y and produced small litters, with 40% of embryos surviving gestation. In this study, we examined the regulation of ACS4 by estradiol-$17{\beta}$ and progesterone (P4) in the human endometrial cancer cell line HTB-1B. ACSL4 mRNA was increased in a dose-dependent manner. Also, expression of ACSL4 gene was up-regulated in a time-dependent manner in HTB-1B cells. However, combined treatment with progesterone and estradiol-$17{\beta}$ modestly decreased the levels of ACS4L mRNA as compared with the estradiol-$17{\beta}$ and progesterone respectively. Overall, these results suggest that the ACSL4 gene is regulated by progesterone and estradiol-$17{\beta}$ in the HTB-1B cells.

Keywords

References

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