• Title/Summary/Keyword: ACP1

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Effect of Testoeterone and Cyclic AMP-theophylline on Ultrastructure and Several Enzymes' Activities in Rat Epididymis (Testosterone과 Cyclic AMP-theophyllin이 흰쥐 부정소의 미세구조 및 몇 가지 효소활성에 미치는 영향)

  • 정경순;최임순
    • The Korean Journal of Zoology
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    • v.31 no.1
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    • pp.35-48
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    • 1988
  • This study was made to investigate the effect of testosterone and cyclic-AMP (cAMP) on rat epididymis. Peritoneai injections of testosterone and cAMP to rats were earned out The activities of acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were measured and ultrastructural changes of luminal epithelial cells were observed. As a result, the activity of ACP was significanily decreased on the third day, that of ALP on the fifth day and that of LDH on the seventh day respectively in castrated group. In addition, the activities of ACP and ALP were significanily increased when treated with testosterone for 5 days, that of LDH when treated with testosterone for 7 days. In case of cAMP and cAMP - theophylline injection, the activities of ACP and LDH were increased but the range of increase was of no significance. However a significant increase in the activity of ALP was seen on both cases. On electron microscopic examination, gradually deformed Golgi complex, destructed mitochondria and disrupted stereociha were observed in castrated group. In case of testosterone injection, disrupted Golgi complex, mitochondria and stereocilia showed recovery. When cAMP and cAMP-theophylline were injected as an alternative, various cytoplasmic organelles as well as Golgi com- plex were recovered but stereocilia remained unrecovered.

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Effects of various toothpastes on remineralization of white spot lesions

  • Jo, Su-Yeon;Chong, Hyun-Jeong;Lee, Eon-Hwa;Chang, Na-Young;Chae, Jong-Moon;Cho, Jin-Hyoung;Kim, Sang-Cheol;Kang, Kyung-Hwa
    • The korean journal of orthodontics
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    • v.44 no.3
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    • pp.113-118
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    • 2014
  • Objective: The purpose of this in vitro study was to examine the effects of fluoridated, casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP)-containing, and functionalized ${\beta}$-tricalcium phosphate (fTCP)-containing toothpastes on remineralization of white spot lesions (WSLs) by using Quantitative light-induced fluorescence (QLF-D) Biluminator$^{TM}$ 2. Methods: Forty-eight premolars, extracted for orthodontic reasons from 12 patients, with artificially induced WSLs were randomly and equally assigned to four treatment groups: fluoride (1,000 ppm), CPP-ACP, fTCP (with sodium fluoride), and control (deionized water) groups. Specimens were treated twice daily for 2 weeks and stored in saliva solution (1:1 mixture of artificial and human stimulated saliva) otherwise. QLF-D Biluminator$^{TM}$ 2 was used to measure changes in fluorescence, indicating alterations in the mineral contents of the WSLs, immediately before and after the 2 weeks of treatment. Results: Fluorescence greatly increased in the fTCP and CPP-ACP groups compared with the fluoride and control groups, which did not show significant differences. Conclusions: fTCP- and CPP-ACP-containing toothpastes seem to be more effective in reducing WSLs than 1,000-ppm fluoride-containing toothpastes.

Modified Suppression Subtractive Hybridization Identifies an AP2-containing Protein Involved in Metal Responses in Physcomitrella patens

  • Cho, Sung Hyun;Hoang, Quoc Truong;Phee, Jeong Won;Kim, Yun Young;Shin, Hyun Young;Shin, Jeong Sheop
    • Molecules and Cells
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    • v.23 no.1
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    • pp.100-107
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    • 2007
  • The moss Physcomitrella patens has two life cycles, filamentous protonema and leafy gametophore. A modified from of suppression subtractive hybridization (SSH), mirror orientation selection (MOS), was applied to screen genes differentially expressed in the P. patens protonema. Using reverse Northern blot analysis, differentially expressed clones were identified. The identified genes were involved mainly in metal binding and detoxification. One of these genes was an AP2 (APETALA2) domain-containing protein (PpACP1), which was highly up-regulated in the protonema. Alignment with other AP2/EREBPs (Ethylene Responsive Element Binding Proteins) revealed significant sequence homology of the deduced amino acid sequence in the AP2/EREBP DNA binding domain. Northern analysis under various stress conditions showed that PpACP1 was induced by ethephon, cadmium, copper, ABA, IAA, and cold. In addition, it was highly expressed in suspension-cultured protonema. We suggest that PpACP1 is involved in responses to metals, and that suspension culture enhance the expression of genes responding to metals.

해외 선진 슈퍼컴퓨팅센터 동향 분석 및 시사점

  • Choe, Jae-Yeong
    • Journal of Scientific & Technological Knowledge Infrastructure
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    • s.13
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    • pp.40-47
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    • 2004
  • 최근 NSF는 Cyberinfrastructure를 통한 과학과 공학의 혁명을 위해 산.학.연 전문가의 의견을 수렴하여 Blue-Ribbon Advisory Panel on Cyberinfrastructure(2003. 1)의 보고서를 통해 ACP(Advanced Cyberinfrastructure Program)을 제안하였다. ACP의 목표는 정보기술을 응용하여 과학 및 공학 연구에 혁신적인 발전을 가져오는데 있다. 본고에서는 미국, 유럽의 대표적 슈퍼컴퓨팅센터인 NCSA와 EPCC(Edenbugh Parallel Computing Center, 영국), HLRS(High Performance Computing Center Stuttgart, 독일)에 대한 분석을 수행해보고자 한다.

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Heritability Estimates of Sink and Source Characters by $F_2$ and $F_5$ Correlation in Rice ($F_2$$F_5$ 상관에 의한 벼 Sink 및 Source 관련형질의 유전력 추정)

  • Ha, Woon-Goo;Kim, Ho-Yeong;Choi, Hae-Chune;Lim, Sang-Jong;Suh, Hak-Soo;Lim, Moo-Sang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.151-156
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    • 2002
  • The heritability of sink-source characters was estimated by regression coefficient between F$_2$ and F$_{5}$ in two crosses of IR 66738-118-1-2/Gayabyeo and the Ilpumbyeo/YR 15965 Acp 33. In order to get the some basic information for breeding of high yielding rice, genetic resources of new plant type with low tiller and heavy panicle were used. Most of the sink and source characters in Ilpumbyeo/YR 15965 Acp 33 cross showed high heritability over than 0.224. But the specific leaf area and sink-source ratio displayed low heritability being 0.009 and 0.013, respectively. Heritability of all sink and source characters in IR 66738-118-1-2/Gayabyeo cross ranged from 0.115 to 0.247. Correlation coefficient between yield and yield components in both combination were in the ranged between 0.001 and 0.247. But correlations among the yield components were not significant. Correlations between the yield and sink-source characters in both combinations were also highly significant. Particularly, correlation between the grain tiling ratio and most of the sink-source characters in IR 66738-118-1-2/Gayabyeo cross were not significant. In Ilpumbyeo/YR 15965 Acp 33 cross, correlations between grain filling ratio and source characters were positively significant. but sink characters (sink capacity and sink-source ratio, etc) were negatively significant.

Nano-Indenter 측정 결과를 Weibull 분포로 해석한 ACP 플라즈마 소스의 플라즈마 에칭 조건에 따른 균일도 연구

  • Kim, Su-In;Lee, Jae-Hun;Kim, Hong-Gi;Kim, Sang-Jin;Seo, Sang-Il;Hwang, Byeong-Hyeon;O, Sang-Ryong;Kim, Nam-Heon;Lee, Chang-U
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.176.1-176.1
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    • 2015
  • 본 연구는 플라즈마 건식 식각 후 박막의 물성 특성 변화 측정에 Nano-Indentation 분석 기법을 도입하였으며, 식각 후 박막 표면 강도를 nano 영역에서 측정하여 박막 표면의 damage 분석에 적용하여 물리적인 해석을 시도하였다. 하지만 기판의 대면적화로 인하여 반도체 공정에 사용되는 기판은 300 mm로 증가하였고 이로 인하여 플라즈마 건식 식각에서 대면적에 대한 균일도 향상 연구를 진행 중에 있다. 이 연구에서는 플라즈마 건식 식각 후 박막의 균일도를 Nano-indenter 측정 결과를 기반으로 Weibull 분포 해석을 통하여 정량적인 균일도를 측정하고자 하였다. 플라즈마 건식 식각을 위하여 플라즈마 소스는 Adaptively Coupled Plasma (ACP)를 사용하였고 식각 후 TEOS $SiO_2$ 박막 표면을 분석하기 위하여, 시료 평면의 x, y 축에 대하여 각각 $20{\mu}m$로 indent 각 지점을 이격하여 동일한 측정 조건에서 Nano-indenter를 이용하여 박막 표면의 강도를 측정하였다. 측정된 결과는 Weibull 분포를 활용하여 정량화하였다. 결과에 의하면 플라즈마 소스의 bias 파워가 300 W 일 때 균일도가 가장 높은 29.84로 측정되었고, 150 W 일 때 가장 낮은 8.38로 측정되었다. 식각 전 TEOS $SiO_2$ 박막의 Weibull 분포에 의한 균일도가 17.93으로 측정됨을 기반으로 ACP 플라즈마 소스의 식각 조건에 따라 TEOS $SiO_2$ 박막의 균일도가 상대적으로 변함을 정량적으로 분석할 수 있었다.

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A 5Watt Power Amplifier Module Using Gallium Nitride Device (질화갈륨소자를 이용한 5Watt급 전력증폭기 모듈)

  • Park, Chun-Seon;Han, Sang-Min;Lim, Jong-Sik;Ahn, Dal;An, Chong-Chul;Park, Ung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.5
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    • pp.1193-1200
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    • 2008
  • This paper describes a developed 5Watt power amplifier module fer mobile communication system using Gallium Nitride (GaN) devices. Three amplification stages such as pre-amplifier, driver amplifier, and power amplifier have been fabricated and measured separately in advance for incorporating the total power amplifier module and estimating the performances. In addition, a defected ground structure is combined with the output stage of the power amplifier module for improving harmonic rejection and adjacent channel power (ACP) characteristics. The measured performances of the GaN power amplifier module include 58dB,min of gain, 37dBm,min of output power, 50dBc,min of harmonic rejection, 35dBc,min of IMD3 for 2-tone input, and 35dBc,min of ACP at 2.1GHz frequency band.

The Effects of Acute Osmotic Stress on Innate Immunity of Nile Tilapia (Oreochromis niloticus)

  • Choi, Sang-Hoon;Park, Kwan-Ha
    • Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.343-349
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    • 2010
  • The effects of osmotic stress on the non-specific immune response of Nile tilapia, Oreochromis niloticus, were investigated. Osmoregulatory mechanism of tilapia has been studied, but less information is available about innate immune response of O. niloticus faced with hyperosmolality. Acute osmotic stress was elicited by transferring tilapia from freshwater (FW) to 24 psu seawater (SW). Non-specific immune parameters including lysozyme activities of plasma and head kidney (HK), alternative complement pathway (ACP) activity in plasma, phagocytic capacities of spleen and HK immune cells, and respiratory burst activity of immune cells in both HK and spleen were analyzed. Lysozyme activities were increased at 1 h and 30 h after transfer to SW, but decreased at 10 h after SW transfer. Conversely, ACP activity increased 10 h after SW transfer. Phagocytic capacity increased slightly at 1 h and 5 h after SW transfer, and respiratory burst activity showed an increase in superoxide release at 10 h after SW transfer. Taken together, these results indicate that the exposure of tilapia to hyperosmotic conditions has immunostimulatory effects on cellular and humoral immune reactions.

Variations of Agronomic Traits on the Progenies of the Different Callus Origin and Subculture Period in Rice (벼에서 캘러스 유기원와 계대배양 기간에 따른 재분화 계통의 주요 농업형질 변이)

  • Yi, Gi-Hwan;Oh, Byeong-Geun;Yang, Sae-Jun;Kim, Soon-Chul;Nam, Min-Hee
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.365-368
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    • 2003
  • Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17%) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1%) in ACP, leaf color (4.2%) in OCP and grain fertility (4.9%) and leaf color (4.9%) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).

Comparison of enzyme cytochemical activities between rosetted cells in peripheral blood of Korean native cattle (한우(韓牛) 말초혈액내(末梢血液內) rosette 형성세포(形成細胞)의 효소화학적(酵素化學的) 염색성(染色性) 비교(比較))

  • Kim, Nyeun-su;Song, Hee-jong;Kim, Sun-jae;Seo, Ye-won
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.129-135
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    • 1997
  • Peripheral blood mononuclear cells(PBMNC) of Korean native cattle rosetted with Korean goat erythrocytes(KGRBC) and blood monocytes were evaluated for four cytochemical reactions such as acid phosphatase(ACP), alkaline phosphatase-anti-boby(ALP-Ab), ${\alpha}$-naphthyl butyrate esterase(${\alpha}$-NBE) and peroxidase. The results obtained were as follows; In rosetted cells, the positivities of ACP in E AET-DeX, EA and EAC were 70.3%, 22.4% and 25.2%, those of ${\alpha}$-NB were 27.4%, 44.2% and 79.8%, and those of ALP-Ab were 9.5%, 88.3% and 91.5%, respectively. Whereas, the positivity for Peroxidase in monocytes was 100%. In non-rosetted (remained) cells, the positivities of ACP in E AET+DeX. EA and EAC were 41.4%, 57.2% and 61.9%, those of ${\alpha}$-NB were 38.6%, 16.5% and 18.9% and those of ALP-Ab were 98.2%, 5.3% and 6.3%, in order.

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