• 원예과학기술지
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• 제31권3호
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• pp.275-281
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• 2013

Low temperature is one of the major environmental factors that affect productivity including reduced growth and budding of vines, and changes of metabolic processes in grape (Vitis spp.). To screen the specific expression of abiotic stress-related genes against cold treatment in 'Kyoho' and 'Campbell Early' grapevines, expression of various defense-related genes was investigated by RT-PCR and real-time PCR. Among the 67 genes analyzed by RT-PCR and real-time PCR, 17 and 16 types of cDNA were up-regulated, while 5 and 6 types were down-regulated in cold-treated 'Kyoho' and 'Campbell Early' grapevines, respectively. Genes encoding carotene (Cart3564 and Cart4472), chalcone isomerase (CHI), cytochrome P450 (CYP), flavonol synthase (FLS), endo-${\beta}$-glucanase precursor (Glu), glutathione peroxidase (GPX), glutathione-S-transferase (GST), leucine-rich repeats (LRR), manganese superoxide dismutase (Mn-SOD), phenylalanine ammonia lyase (PAL), polygalacturonase-inhibiting protein (PGIP), proline rich protein 2 (PRP2), small heat shock protein (sHSP), temperature induced lipocalin (TIL), and thaumatin-like protein (TLP) were up-regulated, while those encoding CBF like transcription factor (CBF1), chitinase-like protein (CLP), cold induced protein (CIP), glycerol-3-phosphate acyltransferase (GPAT), and mitogen-activated protein kinase (MAPK) were down-regulated by low temperature treatment in both in 'Kyoho' and 'Campbell Early'.

• 농업과학연구
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• 제46권4호
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• pp.769-779
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• 2019

Chilli peppers are predominantly cultivated in open field systems under abiotic and biotic stress conditions. Abiotic and biotic factors have a considerable effect on plant performance, fruit quantity, and quality. Chilli peppers grow well in a tropical climate due to their adaptation to warm and humid regions with temperatures ranging from 18 to 30℃. Nowadays, chilli peppers are cultivated all around the world under different climatic conditions, and their production is gradually expanding. Expected climate changes will likely cause huge and complex ecological consequences; high temperature, heavy rainfall, and drought have adverse effects on the vegetative and generative development of all agricultural crops including chilli peppers. To gain better insight into the effect of climate change, the growth, photosynthetic traits, morphological and physiological characteristics, yield, and fruit parameters of chilli peppers need to be studied under simulated climate change conditions. Moreover, it is important to develop alternative agrotechnologies to maintain the sustainability of pepper production. There are many conceivable ideas and concepts to sustain crop production under the extreme conditions of future climate change scenarios. Therefore, this review provides an overview of the adverse impacts of climate change and discusses how to find the best solutions to obtain a stable chilli pepper yield.

• 생명과학회지
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• 제20권2호
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• pp.208-212
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• 2010

작물의 수량과 품질은 저온 및 고온 스트레스에 영향을 받는 것으로 알려져 있다. 본 연구에서는 배추에서의 스트레스 저항성과 연관된 분자마커를 개발하기 위하여 저온에서의 스트레스 저항성에 영향을 미치는 것으로 알려져 있는 alternative oxidase (AOX) 합성 유전자 관련 분자 마커를 개발하였다. 총 15개의 AOX 합성 유전자와 관련된 Brassica rapa ESTs를 arabidopsis AOX 합성 유전자 염기서열을 이용하여 찾을 수 있었다. 이를 이용하여 고온에서 상대적으로 약한 '지부'품종과 상대적으로 강한 '권심' 사이에서 DNA 염기서열을 조사하여 4개의 ESTs에서 insertion 또는 deletion을 찾았고 PCR로 확인 가능한 4개의 공동우성 마커를 개발하였다. 본 연구에서 개발된 분자마커는 배추 작물에서 환경스트레스 저항성과 유전적 연관성을 확인하는데 유용하게 사용될 수 있다고 기대된다.

• BMB Reports
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• 제41권5호
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• pp.376-381
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• 2008

The discovery of RNA silencing inhibition by virus encoded suppressors or low temperature leads to concerns about the stability of transgenic resistance. RNA-dependent RNA polymerase (RdRp) has been previously characterized to be essential for transgene-mediated RNA silencing. Here we showed that low temperature led to the inhibition of RNA silencing, the loss of viral resistance and the reduced expression of host RdRp homolog (NtRdRP1) in transgenic T4 progeny with untranslatable potato virus Y coat protein (PVY-CP) gene. Moreover, RNA silencing and the associated resistance were differently inhibited by potato virus X (PVX) and tobacco mosaic virus (TMV) infections. The increased expression of NtRdRP1 in both PVX and TMV infected plants indicated its general role in response to viral pathogens. Collectively, we propose that biotic and abiotic stress factors affect RNA silencing-mediated resistance in transgenic tobacco plants and that their effects target different steps of RNA silencing.

• 한국해양학회지
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• 제26권2호
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• pp.155-168
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• 1991

만경, 동진강 하구 표영 생태계에서 일차생산자의 군집 구조와 분포 양상 그리고 이들과 무생물 환경과의 관계를 파악하기 위하여 1989년 10월부터 1990년 7월까지 5회 에 걸쳐 시료를 채집, 분석 하였다. 그 결과 식물플랑크톤은 159종이 동정되었으며 규 조류가 70.4%로 가장 우세하였다. 생태특성으로는 연안종이 52%로 가장 우세하였고 담 수종 18%, 외상양 12% 순으로 나타나 담수와 외양의 해수에 의해 영향을 받는 하구역 의 특성이 뚜렷하다. 식물플랑크톤의 종 조성은 염분 변화에 좌우되는 것으로 사료된 다. 연구해역에서 일차생산자의 현존량은 시, 공간에 따라 크게 변하는데 투명도와 밀 접한 관계가 것으로 보이며 서해의 다른 해역에 비해 높은 수준이다. 종 다양성 지수 역시, 시, 공간적으로 변화가 컸으며 10월에 최대, 5월에 최소치를 보였다. 연구해역 은 가을과 겨울에는 외양수에 의한 영향으로, 봄과 여름에는 강으로부터 유입되는 담 수에 의한 영향으로 크게 두 개의 해역으로 구분되어 진다.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.287-295
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• 2017

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.

• 한국환경생태학회지
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• 제20권4호
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• pp.374-390
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• 2006

물리적 구조조사 항목 25개 중에서 하천유형 분류척도로 이용되는 9개의 항목과 출현한 저서생물 및 식물과의 상관성을 분석한 결과 하상재료의 다양성, 하도의 사행성 횡단사주의 발달정도가 생물의 출현에 높은 상관성을 보였다. 그리고 수화학적 인자의 경우 총 12개 중 COD농도만이 저서생물의 분포에 유효한 상관성을 나타내었다. 본 연구의 무생물적 인자와 생물적 인자와의 상관성 분석에서는 특히 모래하천과 자갈하천이 서로 음과 양의 방향으로 상관관계를 나타내어 두개 유형의 하천의 물리적 구조 및 생태적 특성이 서로 뚜렷하게 다르다는 것을 지시하고 있었다. 따라서 이러한 분석결과는 생물서식처를 고려해야만 하는 하천복원이나 하천의 생태적 유형분류를 위한 하천평가에도 활용되어질 수 있다고 사료된다.

• Plant Resources
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• 제8권3호
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• pp.202-208
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• 2005

Osmotic stress is one of major limiting factors in crop production. In particular, seasonal drought often causes the secondary disease in the field, resulting in severe reduction in both quality and productivity. Recent efforts have revealed that many genes encoding protein kinases play important roles in osmotic stress signal transduction pathways. Previously, the AtSIK (Arabidopsis thaliana Stress Inducible Kinase) mutants have shown to enhance tolerance to abiotic stresses, accompanying with higher expression of abiotic stress-related genes than did the wild-type plants. In this study, we have transformed potato (cv. Taedong Valley) with the AtSIK expression cassette. Both PCR and RT-PCR using AtSIK-specific primers showed stable integration and expression of the AtSIK gene in individual transgenic lines, respectively. Foliar application of herbicide ($Basta^{(R)}$) at commercial application rate (0.3% (v/v)) revealed another evidence of stable gene introduction of T-DNA which includes the bar gene for herbicide resistance. Overexpression of the AtSIK gene under dual CaMV35S promoter increased sensitivity to salt stress (300 mM NaCl), which was demonstrated by the reduction rate of chlorophyll contents in leaves of transgenic potato lines. These results suggest that possible increase of osmotic tolerance in potato plants may be achieved by antisense expression of AtSIK gene.

• The Plant Pathology Journal
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• 제26권2호
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• pp.101-109
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• 2010

Protein phosphorylation is one of the major mechanisms for controlling many cellular processes in all living organisms. Mitogen-activated protein kinase (MAPK) cascades are known to transducer extracellular stimuli to several cellular processes, including cell division, differentiation as well as responses to various stresses. In plants, several studies have revealed that MAPK cascade pathways play an important role in responses against biotic and abiotic stresses, including wounding, pathogen infection, temperature, drought, salinity and plant hormones. It is also known that MAPK cascades-mediated signaling is an essential process in the resistance step to pathogens by regulating the activity of transcription factors. Here, the insights into the functions of MAPK cascade pathways in plant defense response signaling from Arabidopsis, tobacco and rice are described.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.69-76
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• 2002

Mustard aphid, Lipaphis erysimi(Kalt.) plays a key role in determining the productivity of rapeseed-mustard. This pest alone attributes 30-70% losses in yield potential in different agro climatic zones with a mean loss of 54.2% in India. Considering the extent of damage caused by the pest, the present experiment was conducted at Pulses and Oilseeds Research Station during 1992-93 to 1994-95 to study the migratory nature of mustard aphid by trapping them in yellow colour pan tray and their simultaneous growth and development on three different genotypes. The temperature during noon hours is the predominant factor to govern the appearance of alate mustard aphid in rapeseed-mustard field. Stepwise regression analysis revealed that temperature and relative humidity played an important role for its development. The variety RW white flower glossy stem harboured minimum number of aphid in comparison to other two varieties B 9 and T6342. The population reached a peak of 61.28 aphids/10 cm central twig during $6^{th}$ standard week irrespective of varieties.