Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.2.208

Development of Molecular Markers for Alternative Oxidase Synthesis Genes in Brassica rapa L.  

Jeong, Ye-Sol (Department of Life Science, Dongguk University-Seoul)
Chung, Sang-Min (Department of Life Science, Dongguk University-Seoul)
Publication Information
Journal of Life Science / v.20, no.2, 2010 , pp. 208-212 More about this Journal
Abstract
The low and high temperature stress might affect the yield and quality of many crop species. Alternative oxidase (AOX) gene is known as factors related to stress resistance in plants. In order to develop molecular markers related to stress resistance in Chinese cabbage, fifteen ESTs sharing sequence similarity to arabidopsis AOX genes were found using Brassica rapa EST database from NCBI. The polymorphic DNA sequences using the ESTs were then screened between Chinese cabbage, 'Chiifu' and 'Kenshin'. We found four ESTs that have either insertion or deletion between the two cultivars. These polymorphic sites were then targeted for development of the four PCR based molecular markers. These molecular markers developed in this study could be useful for a test of their relationship with abiotic stress resistance in Chinese cabbage.
Keywords
Chinese cabbage; abiotic stress; alternative oxidase; molecular marker;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, Y. S., Z. B. Zheng, and D. H. Shin. 2008. Growth inhibitory effects of kimchi (Korean traditional fermented vegetable product) against Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus. J. Food Prot. 71, 325-332.
2 Kreps, J. A., Y. Wu, H. S. Chang, T. Zhu, X. Wang, and J. F. Harper. 2002. Transcriptome changes for arabidopsis in response to salt, osmotic, and cold stress. Plant Physiology 130, 2129-2141.   DOI
3 Labana, K. S. and M. L. Gupta. 1993. Importance and origin, pp. 1-20, In K. S. Labana, S. S. Banga, and S. K. Banga (eds.), Breeding Oilseed Brassicas, Springer-Verlag Press, Berlin.
4 Lagerctantz, U. and D. Lydiate. 1996. Comparative genome mapping in brassica. Genetics 144, 1903-1910.
5 Levadoux, W. L., M. L. Kalmokoff, M. D. Pickard, and J. W. D. GrootWassink. 1987. Pigment removal from canola oil using chlorophyllase. J. Am. Oil Chem. Soc. 64, 139-144.   DOI
6 Mahmud, T. M. M., J. G. Atherton, C. J. Wright, M. F. Ramlan, and S. H. Ahmad. 1999. Pak Choi (Brassica rapa ssp. Chinensis L.) quality response to pre-harvest salinity and temperature. J. Sci. Food Agric. 79, 1698-1702.   DOI
7 Maxwell, D. P., Y. Wang, and L. Mcintosh. 1999. The alternative oxidase lower mitochondrial reactive oxygen production in plant cells. Proc. Natl. Acad. Sci. USA 96, 8271-8276.   DOI
8 McDonald, A. E. and G. C. Vanlerberghe. 2004. Branched mitochondrial electron transport in the animalia: Presence of alternative oxidase in several animal phyla. IUMBM Life 56, 333-341.   DOI
9 Nagaharu, U. 1935. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn. J. Bot. 7, 389-452.
10 Prasad, M., R. K. Varshney, J. K. Roy, H. S. Balyan, and P. K. Gupta. 2000. The use of microsatellite for detecting DNA polymorphism, genotype identification and genetic diersity in wheat. Theor. Appl. Genet. 100, 584-592.
11 Daisuke, S., E. Nambara, S. Naito, N. Tsutsumi, A. Hirai, and M. Nakazono. 1997. Charactaerization of the gene family for alternative oxidase from Arabidopsis thaliana. Plant Molecular Biology 35, 585-596.   DOI
12 Abe, F., K. Saito, K. Miura, and K. Toriyama. 2002. A single nucleotide polymorphism in the alternative oxidase gene among rice varieties differing in low temperature tolerance. FEBS Lett. 527, 181-185.   DOI
13 Ayeh, K. O. 2008. Expressed sequence tags (ESTs) and single nucleotide polymorphism (SNPs): Emerging molocular marker tools for improving agronomic traits in plant biotechnology. Afr. J. Biotechnol. 7, 331-341.
14 Berhold, D. A. and P. Stenmark. 2003. Membrane-bound diiron carboxylate proteins. Annu. Rev. Plant Biology 54, 497-517.   DOI
15 Kim, M. J. and J. S. Chun. 2005. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int. J. Food Microbiol. 103, 91-96.   DOI
16 Vanlerberghe, G. C. and L. McIntosh. 1997. Alternative oxidase: From gene to function. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 703-734.   DOI
17 Sugie, A., N. Naydenov, N. Mizuno, C. Nakamura, and S. Takumi. 2006. Overexpression of wheat alternative oxidase gene Waox1a alters respiration capacity and response to reactive oxygen species under low temperature in transgenic Arabidopsis. Genes Genet. Syst. 81, 349-354.   DOI
18 Thomashow, M. F. 1998. Role of cold-responsive genes in plant freezing tolerance. Plant Physiol. 118, 1-8.   DOI
19 Umbach, A. L., F. Fiorani, and J. N. Siedow. 2005. Characterization of transformed arabidopsis with altered alternative oxidase levels and analysis of effects on reactive oxygen species in tissue. Plant Physiol. 139, 1806-1820.   DOI