• 한국전기전자재료학회논문지
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• 제15권9호
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• pp.743-749
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• 2002

A 1.5V 70㏈ 100MHz CMOS class-AB complementary operational amplifier is presented. For obtaining the high gain and the high unity gain frequency, the input stage of the amplifier is designed with rail-to-rail complementary differential pairs which are symmetrically parallel-connected with the NMOS and the PMOS differential input pairs, and the output stage is designed to the rail-to-rail class-AB output stage including the elementary shunt stage technique. With this design technique for output stage, the load dependence of the overall open loop gain is improved and the push-pull class-AB current control can be implemented in a simple way. The designed operational amplifier operates perfectly on the complementary mode with 180$^{\circ}$ phase conversion for 1.5V supply voltage, and shows the push-pull class-AB operation. In addition, the amplifier shows the DC open loop gain of 70.4 ㏈ and the unity gain frequency of 102 MHz for $C_{L=10㎊∥}$ $R_{L=1㏁}$ Parallel loads. When the resistive load $R_{L}$ is varied from 1 ㏁ to 1 ㏀, the DC open loop gain of the amplifier decreases by only 2.2 ㏈.a$, the DC open loop gain of the amplifier decreases by only 2.2 dB.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2009년도 춘계학술대회 논문집
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• pp.248-251
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• 2009

AB100 & CB100 are general products in the steel beam of the section shape. They are applied for construction, welding and shipbuilding structures. But currently roll damages are generated in the intermediate & finish mills during producing AB 100, CB100. The goal of this project is to conduct rolling & roll stress analysis using the 3D FEM program and to research the things which roll diameter affects roll stress. By the analysis results, it is determined that the recording roll stress where the roll diameter will be big comes to be small, and it is recognized that roll damage occurrences of finish rolling mills can be high by roll stress analysis results.

• 대한의생명과학회지
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• 제26권3호
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• pp.136-148
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• 2020

A bispecific antibody (BsAb) is an artificial protein containing two kinds of specific antigen binding sites. BsAb can connect target cells to functional cells or molecules, and thus stimulate a directed immune response. Last several decades a wide variety of bsAb formats and production technologies have been developed. BsAbs are constructed either chemically or biologically, exploiting techniques like cell fusion and recombinant DNA technologies. There are over 100 different formats of bsAb so far developed, but they could be classified into the two main categories such as Fc-based (with a Fc region) bsAbs and fragment-based (without a Fc region) bsAbs. BsAb has a broad application prospect in tumor immunotherapy and drug delivery. Here, we present a brief introduction to the structure of antibody, pharmacological mechanisms of antibodies and the trend in the production technologies of therapeutic antibodies. In addition, we address a review on the current status of various bsAb format development and their production technologies together with global situation in the clinical studies of bsAb.

• BMB Reports
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• 제30권3호
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• pp.211-215
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• 1997

A photoactive analog of ATP, 3'(2')-O-(p-azidobenzoyl)-adenosine 5-triphosphate (AB-ATP) was synthesized by chemically coupling N-hydroxysuccinimidyl-4-azidobenzoate (NHS-AB) and ATP. The utility of AB-ATP as an effective active-site-directed photoprobe was demonstrated using catalytic subunit of protein kinase A as a model enzyme. Photoincorporation of AB-ATP was saturated with apparent dissociation constant of $30{\mu}m$ and protected completely by $100{\mu}m$ of ATP. When the enzyme was covalently modified by photolysis in the presence of saturating amounts of photoprobe, about 60% inhibition of enzyme activity was observed. These results demonstrate that AB-ATP has potential application as a probe to characterize ATP-binding proteins including protein kinases.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.959-962
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• 2013

Objective: HBV infection may cause damage to the immune system and induce lymphomas as a result. Some scholars have indicated that HBsAg(+) reflecting HBV infection may have a relationship with lymphoma development. This study was designed to find out the specific stage of HBV infection which may be related to lymphoma. Methods: HBV serum markers, including HBsAg, HBsAb, HBeAg, HBeAb, HBcAb were tested among 100 lymphoma patients and 100 other patients who were diagnosed with non-lymphoma diseases in the First Hospital of Jilin University from 2010.1.1 to 2012.12.31. Three subgroups were established depending on different combinations of HBV serum markers. Subgroup 1 was HBsAg(+) representing the early stage of HBV infection. Subgroup 2 was HbsAb(+) representing convalescence and Subgroup 3 was "HbsAg and HbsAb negative combined with other positive markers" representing the intermediate stage of HBV infection. Chi square tests were used to compare the rates of three subgroups in lymphoma and control groups. Results: The rates of Subgroup were 13% and 5% respectively, an association between HBsAg and lymphoma being found (P<0.05). There was no difference between rate of Subgroup 2 of lymphoma group (15%) and that of control group (16%). In lymphoma group and control group, the rate of Subgroup 3 was different (12% vs 4%). This evidence was not specific to T cell lymphoma, B cell lymphoma or Hodgkin's lymphoma. Conclusions: Among serum markers of HBV, the combination of serum markers representing the early stage and intermediate stage of HBV infection have a relationship with lymphoma. Convalescence from HBV infection appears to have no relationship with lymphoma.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.179-181
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• 2003

$F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.

• 치과방사선
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• 제21권1호
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• pp.99-107
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• 1991

저자는 두부규격방사선사진 촬영기에서 건조 두개골 10개의 basal view와 lateral view상에서 측두골의 추체와 접형골의 후상상돌기가 악관절부위에 중첩되지 않는 수평면과 수직면에서의 입사각을 구하였고 Denar사의 Accurad-100의 형태대로 제작하고 고정장치와 cephalostat를 사용하여 촬영된 방사선사진의 재현성을 검토하여 다음과 같은 결과를 얻었다. 중심방사선의 입사각은 수직면에서는 24.5±4.1°, 수평면에서는 3.0±5.7°였으며 재현성을 검정하기 위해 건조두개골의 악관절부위에 임의의 3점을 계측하였든 바 Accurad-100의 형태대로 제작한 고정장치를 이용한 경우에는 AB간의 거리 24.60±0.25㎜, AC간의 거리 16.76±0.27㎜, BC간의 거리 8.79±0.18㎜이었고 cephalostat를 이용한 경우에는 AB간의 거리 29.03±0.14㎜, AC간의 거리 19.95±0.24㎜, BC간의 거리 10.08±0.07㎜로 나타났다. 따라서 이들은 상당히 양호한 재현성을 나타냈으며 특히 cephalostat를 이용한 경우 더욱 재현성이 좋았다.

• 한국임상수의학회지
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• 제31권1호
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• pp.40-45
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• 2014

Abalone herpes-like virus (AbHV) is a fatal disease of abalones that impose severe economic impacts on the industry of infected regions due to high mortality. The aim of this study was to quantify the risk of introducing AbHV into Korea through the importation of live abalones for human consumption by import risk analysis (IRA). Monte Carlo simulation models were developed to provide estimates of the probability that a ton of imported abalone contains at least one AbHV-infected individual, using historical trade data and relevant literatures. A sensitivity analysis with 5,000 iterations was also conducted to determine the extent to which input parameters affect the outcome of the model. Although many uncertainties were present in the data, the results indicated that, if 5,000 tons of abalone were imported from a hypothetical exporting country with low prevalence of AbHV (model 1), there would be at least one AbHV-infected abalones in 4,816 of those tons (96.3%), while there would be at least one AbHV-infected abalones in 100% of those tons imported from country with high prevalence (model 2). Sensitivity analysis indicated that for model 1, prevalence was the strongest influence factor on the predicted number of infections. For model 2, background mortality and washing to reduce the risk of surface contamination during processing were the major contributing factors. Risk management strategies need to be enforced to reduce the risk of AbHV introduction in that at least one infected abalone would remain in a consignment from country even with a low prevalence of AbHV infection. The methodology and the results presented here will contribute to improve the development of AbHV management program, and with more accurate data this IRA model will aid science-based decision-making on mitigation strategies to reduce the risk of AbHV introduction in Korea.

• Restorative Dentistry and Endodontics
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• 제21권2호
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• pp.530-545
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• 1996

The purpose of this study was to investigate by spectrophotometry the changes in color parameters of composite resins after exposure to distilled water, filtered coffee, and artificial saliva. Five kinds of fine particle composite resin in experiment 1, and six kinds of composite resin in experiment 2 were used. In experiment 1, each group of composite resin was stored in distilled water at $4^{\circ}C$, $50^{\circ}C$, and filtered coffee solution at $4^{\circ}C$, $50^{\circ}C$. And then each specimen was measured by spectrophotometer. Measurements were repeated in 1 hour, 2 hours, 4 hours, 8 hours, 16 hours, 24 hours, and 48 hours. In experiment 2, all specimens of each brand were stored in distilled water at $37^{\circ}C$, artificial saliva not contained mucin at $37^{\circ}C$, and artificial saliva contained mucin at $37^{\circ}C$. All specimens of each brand were measured by spectrophotormeter in 1 week, 2 weeks, 4 weeks, 8 weeks, and up to 12 weeks. The results were as follows : 1. In cold coffee, five of fine particle composite resins showed ${\Delta}E{^*}_{ab}$ values less than 2. However, in hot coffee ${\Delta}E{^*}_{ab}$ values of Amelogen Universal and Prisma TPH were higher than those of Conquest Crystal, ${\AE}$litefil and Z100. 2. Z100 and ${\AE}$litefil had better cleansibility of extrinsic coffee staining than Amelogen Universal, Conquest Crystal and Prisma TPH. 3. In distilled water and artificial saliva not contained mucin, five of fine particle composite resin showed ${\Delta}E{^*}_{ab}$ values less than 2 up to 12 weeks. However, Silux Plus showed higher than ${\Delta}E{^*}_{ab}$ values of fine particle composite resin. 4. As an immersion solution for discoloration experiment, artificial saliva not contained mucin showed similar appearance as distilled water. However, artificial saliva contained mucin had different appearance from the others. 5. In artificial saliva contained mucin, ${\AE}$litefil, Prisma TPH and Z100 showed ${\Delta}E{^*}_{ab}$ values less than 1 up to 12 weeks. However, Silux Plus, Amelogen Universal and Conquest Crystal showed ${\Delta}E{^*}_{ab}$ values more than 1 up to 12 weeks. But, Conquest Crystal showed different characteristics of chromacity difference value(less chromatic) and lightness difference value(lighter) from Silux Plus and Amelogen Universal.

• Clinical and Experimental Pediatrics
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• 제50권4호
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• pp.355-362
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• 2007

목 적 : 3가 항원 DTaP 백신을 3회 기초 접종 받은 건강한 국내 영아에서 면역원성 및 안전성을 평가하고자 하였다. 방 법 : 2004년 4월부터 2005년 4월까지 5개 연구 참여 대학병원에서 제태기간 32주 이상에서 태어난 건강한 영아에게 생후 2, 4, 6개월에 3가 항원 DTaP 백신($Infanrix^{(R)}$)을 대퇴부 전외측에 접종하여 접종 전 혈청과 3차 접종 1개월 후 혈청에서 디프테리아, 파상풍, PT, FHA, PRN 항원에 대한 항체를 측정하여 면역원성을 평가하였고, 접종 후 3일 내에 발생이 예상되는 국소 및 전신 이상반응을 적극 관찰하였고 3주내에 발생한 모든 이상반응을 관찰하여 안전성 평가를 실시하였다. 한편, 2가 항원 DTaP 백신을 접종 받은 14명을 대조군으로 설정하여 연구군과 동일한 방법으로 면역원성을 평가하여 비교하였다. 결 과 : 60명에서 실시된 연구군의 면역원성 평가에서 디프테리아, 파상풍, FHA, PRN 항원에 대한 항체 양전율은 100%이었고, PT 항원에 대한 항체 양전율은 98.3%이었다. 한편 대조군에서 디프테리아, 파상풍, PT, FHA 항원에 대한 항체 양전율은 100%이었으나 PRN 항원에 대한 항체 양전율은 50%이었다. 147명에서 확인된 이상반응 평가에서 연구 기간동안 연구 백신과 인과관계가 있는 중증 이상반응은 없었고, 3일 내에 특이한 치료 및 처치가 없이 자연 소멸되는 국소 및 전신 이상반응만 관찰되었으며 접종 차수별 이상반응의 증가도 없었다. 결 론 : 이상의 결과를 볼 때에 3가 항원 DTaP 백신($Infanrix^{(R)}$)은 국내 영아 기초 접종에 유효한 백신임을 확인할 수 있었고, 국내에서 가장 많이 사용되고 있는 2가 DTaP 백신에 비해 PRN항원에 대한 항체의 면역원성이 높은 이점이 있음을 확인하였다. 그러나 향후 더욱 많은 대상을 통한 연구가 이루어져 이런 결과를 재입증하여야 할 것이고, 추가 접종에 대한 동일한 연구도 요구된다.