BMB Reports
- Volume 30 Issue 3
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- Pages.211-215
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- 1997
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- 1976-670X(eISSN)
Synthesis and Photoaffinity Labeling of 3'(2')-O-(p-azidobenzoyl) ATP
- Shin, Seung-Jin (Center for Molecular Catalysis, Department of Chemistry, Seoul National University) ;
- Lee, Woo-Kyoung (Center for Molecular Catalysis, Department of Chemistry, Seoul National University) ;
- Park, Jong-Sang (Center for Molecular Catalysis, Department of Chemistry, Seoul National University)
- Received : 1997.03.15
- Published : 1997.05.31
Abstract
A photoactive analog of ATP, 3'(2')-O-(p-azidobenzoyl)-adenosine 5-triphosphate (AB-ATP) was synthesized by chemically coupling N-hydroxysuccinimidyl-4-azidobenzoate (NHS-AB) and ATP. The utility of AB-ATP as an effective active-site-directed photoprobe was demonstrated using catalytic subunit of protein kinase A as a model enzyme. Photoincorporation of AB-ATP was saturated with apparent dissociation constant of