• Title/Summary/Keyword: A375-S2

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DNA fingerprinting patterns of 5 serotypes of Actinobacillus actinomycetemcomitans (Actinobacillus actinomycetemcomitans의 혈청형별 제한절편장 다변화에 관한 연구)

  • Choi, Jeom-Il;Koh, Myung-Yun;Yun, Il
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.365-375
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    • 1996
  • 5 serotypes(a, b, c, d, e) of Actinobacillus actinomycetemcomitans showed distinct hybridization patterns(DNA fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7-Kb sized DNA probe. The sizes of hybridized bands in each serotypes were different among serotypes and represented unique patterns of hybridization with the probe used. The serotype a showed two bands of fingerprinting patterns: 23.1 kb and 2.5 kb respectively. Serotype b and c showed single band: 6.6 kb and 9.5 kb, respectively. Serotype d and e showed two bands of hybridization: 23.1 kb and 2.8 kb, and 23.7 kb and 2.1 kb, respectively. The results indicate that this standard fingerpriting patterns of DNA hybridization with 4.7 kb probe can be further used for genotyping clinical isolates of Actinobacillus 8ctinomycetemcomitansand its relevance with periodontal disease activity.

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Magnetic and magneto-optical properties of two metallic phase magnet Co/Co$_2$TiSn films

  • Kim, T. W.;Lee, J. W.;S. C. Shin
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.06a
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    • pp.375-377
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    • 1998
  • The magneto-optical properties of Co/Co$_2$TiSn two-phase magnet films were studied. These films show that relatively large Kerr rotations which are -0.4 deg. at the wavelength of 400 nm, compared to that of pure Co. It is conceivable that the magneto-optical effects may be due to both contributions of ferromagnetic Co matrix and ferromagnetic Co$_2$TiSn Heusler alloy precipitate. The perpendicular magnetization curve domonstrates a typical bubble domain hysteresis loop. the saturation magnetization change of the annealed film is less sensitive to temperature in the low temperature region and the Curie temperature of Co$_2$TiSn Heusler alloy precipitate is a little higher in the annealed film. These can be explained by the increase of the number of Co-Co exchange interaction in Heusler alloy structure resulting from the change of chemical ordering by annealing.

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Experimental and CFD Simulations of Polluted Air Behavior in Rectangular Tunnels

  • Lee, Yong-Ho
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.5
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    • pp.608-615
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    • 2011
  • The objective of this study is to investigate the flow characteristics of polluted air behavior in rectangular tunnels using a PIV system and a commercial CFD program. The PIV experiments are simulated by using the olive oil as the tracer particles in scaled rectangular tunnels. Each model has one of four different outlet vents, each dimensionless L/H ratio of which is 0, 0.375, 0.75 and 1.125, respectively as the locations of each outlet are away from the vertical centerline through the inlet. A commercial CFD program, ANSYS CFX, was used to examine the velocity fields and the pressure distributions in numerical simulations. The kinematic viscosity of the air flow of $1.51{\times}10^{-5}m^2/s$ and the flow velocity of 0.3 m/s at the inlet are given under the same conditions in order to analyze the polluted air flow characteristics experimentally and computationally. This study is considered to examine the effect of the outlet locations in the naturally ventilated tunnel models.

Effect of Employed Mother's Work-mother Role Conflict on Parenting Stress: Mediation Effect of Parental Satisfaction (취업모의 일-어머니 역할 갈등이 양육스트레스에 미치는 영향: 부모역할만족도의 매개효과)

  • Kim, An-Na;Kwon, Young-Sook
    • Journal of Digital Convergence
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    • v.12 no.10
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    • pp.375-384
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    • 2014
  • This study was carried out to investigate the mediating role of parental satisfaction on the relationship between work-mother role conflict and parenting stress of employed mothers. Participants were 515 mothers of children aged 2 to 5 recruited from companies in Gyeonggi Province, Korea. Survey was conducted from February 15 to March 20, 2013. Structural equation modeling was used to examine direct and indirect effects among study variables. As a result, work-mother role conflict and parental satisfaction directly influenced parenting stress. Work-mother role conflict directly influenced parental satisfaction. The indirect effect of work-mother role conflict, with parental satisfaction as a mediator was verified on parenting stress. Therefore, to relieve the parenting stress of employed mothers, diverse programs to improve parental satisfaction need to be utilized along with decreasing work-mother role conflict through the settlement of child care policy.

Mutagenicity of the Essential Oils in Ames Test (Ames Test에 의한 정유의 돌연변이원성)

  • Park, Hee-Juhn
    • Korean Journal of Pharmacognosy
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    • v.33 no.4 s.131
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    • pp.372-375
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    • 2002
  • Mutagenic acivity of essential oils was tested using Salmonella typhimurium TA100 in the presence or absence of 59 fraction prepared from the mouse liver. Growth inhibitory effect of the oils on the bacteria was measured to warrant the mutagenic effect. Most oils were (round to be very strongly toxic against the bacteria at a high dose $(2,000{\mu}g/plate)$. At lower doses than this concentration, the Curcuma longa oil was found to be the most mutagenic with S9 fraction whereas it was not mutagenic without the fraction suggesting that this oil could undergo activation for the mutagenicity by cytochrome P45O. However, the mutagenicity of the Eugenia caryohpylata oil was disappeared under S9 fraction. Other oils obtained from Cinnamomum cassia, Chrysanthemum sibiricum, Paeonia moutan the flower of Artemisia princeps var. Orientalis, Allium sativum, were not mutagenic. This result suggested that antimutagenicity assay on the essential oil is necessary for the biological available substances.

Surfacing Process of Pulsed Nd:YAG laser by using Multiple mesh and Pulse Superposition Technique (다단메쉬 및 펄스중첩법을 적용한 펄스형 Nd:YAG의 Surfacing Process)

  • Joung, J.H.;Hong, J.H.;Kim, D.H.;Kim, H.J.;Cho, J.S.
    • Proceedings of the KIEE Conference
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    • 1997.11a
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    • pp.373-375
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    • 1997
  • In this study, we designed multiple mesh circuit consisting of 3-6 meshes and pulse superposition one consisting of a 3 mesh, and fabricated the electrical power supply and the single elliptical resonator. We developed the two pulse superposition technique forming the step pulse shapes of pulsed Nd:YAG laser with single shot multivibrator and 2 SCRs. Laser beam generated by multiple mesh circuit and superposition one respectively irradiated target surface to analyze process state of surface with spark and vapor. And it was obtained experimental results that all superposition meshes had common points which the best efficiency was obtained at delay time 0[${\mu}s$], followed by, no superposition and obtained at delay time 250[${\mu}s$].

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A Structural Model for Chemotherapy Related Cognitive Impairment and Quality of Life in Breast Cancer Patients (유방암 환자의 항암화학요법 관련 인지기능 변화와 삶의 질의 구조모형)

  • Lee, Jung Ran;Oh, Pok Ja
    • Journal of Korean Academy of Nursing
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    • v.49 no.4
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    • pp.375-385
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    • 2019
  • Purpose: This study aimed to develop and test a structural model for chemotherapy-related cognitive impairment of breast cancer patients based on a literature review and Hess and Insel's chemotherapy-related cognitive change model. Methods: The Participants consisted of 250 patients who were ${\geq}19$ years of age. The assessment tools included the Menopause Rating Scale, Symptom Experience Scale, Hospital Anxiety and Depression Scale, Everyday Cognition, and Functional Assessment of Cancer Therapy-Breast Cancer. Data were analyzed using the SPSS 21.0 and AMOS 21.0 programs. Results: The modified model was a good fit for the data. The model fit indices were ${\chi}^2=423.18$ (p<.001), ${\chi}^2/df=3.38$, CFI=.91, NFI=.91, TLI=.89, SRMR=.05, RMSEA=.09, and AIC=515.18. Chemotherapy-related cognitive impairment was directly influenced by menopausal symptoms (${\beta}=.38$, p=.002), depression and anxiety (${\beta}=.25$, p=.002), and symptom experiences (${\beta}=.19$, p=.012). These predictors explained 47.7% of the variance in chemotherapy-related cognitive impairment. Depression and anxiety mediated the relations among menopausal symptoms, symptom experiences, and with chemotherapy related cognitive impairment. Depression and anxiety (${\beta}=-.51$, p=.001), symptom experiences (${\beta}=-.27$, p=.001), menopausal symptoms (${\beta}=-.22$, p=.008), and chemotherapy-related cognitive impairment (${\beta}=-.15$, p=.024) had direct effects on the quality of life and these variables explained 91.3%. Conclusion: These results suggest that chemotherapy-related toxicity is highly associated with cognitive decline and quality of life in women with breast cancer. Depression and anxiety increased vulnerability to cognitive impairment after chemotherapy. Nursing intervention is needed to relieve chemotherapy-related toxicity and psychological factor as well as cognitive decline for quality of life in patients undergoing chemotherapy.

Influence of the Size of the Spoon on the Eating rate, Energy Intake and the Satiety Levels of Female College Students (숟가락 크기의 감소가 여대생의 식사속도, 음식섭취량과 포만도에 미치는 영향)

  • Hong, Yang Hee;Kim, Young Suk;Kwon, Hyun Jung;Chang, Do Seok;Kim, Dong Geon;Chang, Un Jae
    • Korean Journal of Community Nutrition
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    • v.20 no.5
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    • pp.375-382
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    • 2015
  • Objectives: This study examined the influence of different sizes of spoons (normal spoon, 8.3 cc vs small spoon, 4 cc) on eating rate, energy intake and the satiety levels of female college students. Methods: Twenty four healthy female college students participated in this study once a week for 2 weeks. Two hundred ten grams of cooked rice and 250 g of beef shank soup with a normal spoon and same amount of rice and soup with a small size spoon were served to the same participants over two consecutive weeks. After each lunch, the eating rate, energy intake, and the satiety levels were measured. Results: Results showed that the subjects who were using a small spoon ate less beef shank soup (149.0 kcal) (p < 0.01) and had lower total energy intake (423.3 kcal) (p < 0.05) than using a normal spoon (178.7 and 461.1 kcal, respectively). Also, the meal time (15.7 min) (p < 0.01), a serving per one spoon (8.6 g) (p < 0.001), and eating rate (27.9 g/min) (p < 0.001) of those who used a small spoon were significantly different than that of those who used a normal spoon (13.6 min, 12.5 g and 35.7 g/min, respectively). However, despite consuming less energy at lunch, the level of satiety after eating from the small spoon was not significantly different from the normal spoon immediately after, 1 hour after and 2 hour after lunch. Conclusions: Our results revealed that students were able to control their eating rate by using a small spoon and they could feel full enough even though they eat less. In conclusion, eating rate decrease by using a small spoon may play an important role in food intake.

Up-regulation of dynamin-2 gene expression in Ras-transformed cells (Ras에 의해 암화된 세포에서 dynamin-2의 발현 촉진)

  • Yoo, Ji-Yun
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.375-380
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    • 2007
  • Dynamin plays a key role in the scission event common to various types of endocytosis. It has been previously reported that the SH3 domain-mediated association of Grb2 with dynamin-2 was dominantly found in Ras transformed cells. However, whether this association results from the increased expression of dynamin-2 and Grb2 in Ras transformed cells or not is still unknown. So in this study we first analyzed the expression levels of dynamin-2 and Grb2 and found that the expression of dynamin-2 protein was dramatically increased in Ras-transformed NIH3T3 (NIH3T3(Ras)) cells. Furthermore competitive PCR data revealed that the mRNA transcripts for dynamin-2 were increased about 100-fold in NIH3T3(Ras) compared to those of NIH3T3 cells. However, the protein level and mRNA transcript of Grb2 were not changed in these two cells. We also examined promoter activity of dynamin-2 in NIH3T3(Ras) cells and suggest the existence of Ras-responsive sequence in promoter region -300 to -200.

Comparative Study on the Effects of Korean and Chinese Crataegus pinnatifida on Scopolamine-induced Memory Impairment in Mice (Scopolamine 유발 기억 손상 마우스 모델에서 국산 산사와 중국산 산사의 항건망 효과 비교)

  • Lee, Jihye;Kim, Hye-Jeong;Lee, Chanhee;Park, Sang Hyeok;Jung, Chul Jong;Beik, Gyung Yun;Shin, Jin Gi;Jung, Ji Wook
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.32 no.6
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    • pp.375-383
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    • 2018
  • This study was conducted to investigate the cognitive improvement and memory recovery effects of Korean and Chinese Crataegus pinnatifida ethanolic extracts on scopolamine-induced memory impairment in mice. In vivo studies were carried out with mice treated with Korean Crataegus pinnatifida extracts (KCF) and Chinese Crataegus pinnatifida extracts (CCF) in doses of 5 and 50 mg/kg (p.o.) and scopolamine was injected 30 min before the behavioral testing. Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and acetylcholinesterase inhibition by Ellman's modified method. The chlorogenic acid and hyperoside as marker compounds of KCF and CCF was quantified by ultra-performance liquid chromatography analysis (UPLC). Results showed that KCF was more contained high content of chlorogenic acid and hyperoside than CCF. In addition, KCF was more exerted free radical (DPPH and ABTS) scavenging activity and blocked AChE activity than CCF. In vivo studies also showed that KCF administration has a further improved the memory of scopolamine-treated mice than CCF in Y-maze test, passive avoidance test and Morris water maze test. These results revealed that KCF more prevents scopolamine-induced memory impairments through antioxidant and acethylcholinesterase inhibition effect compared CCF.