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http://dx.doi.org/10.5352/JLS.2007.17.3.375

Up-regulation of dynamin-2 gene expression in Ras-transformed cells  

Yoo, Ji-Yun (Department of Microbiology/Research institute of Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 375-380 More about this Journal
Abstract
Dynamin plays a key role in the scission event common to various types of endocytosis. It has been previously reported that the SH3 domain-mediated association of Grb2 with dynamin-2 was dominantly found in Ras transformed cells. However, whether this association results from the increased expression of dynamin-2 and Grb2 in Ras transformed cells or not is still unknown. So in this study we first analyzed the expression levels of dynamin-2 and Grb2 and found that the expression of dynamin-2 protein was dramatically increased in Ras-transformed NIH3T3 (NIH3T3(Ras)) cells. Furthermore competitive PCR data revealed that the mRNA transcripts for dynamin-2 were increased about 100-fold in NIH3T3(Ras) compared to those of NIH3T3 cells. However, the protein level and mRNA transcript of Grb2 were not changed in these two cells. We also examined promoter activity of dynamin-2 in NIH3T3(Ras) cells and suggest the existence of Ras-responsive sequence in promoter region -300 to -200.
Keywords
dynamin-2; Grb2; NIH3T3(Ras); NIH3T3; endocytosis;
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