• Bulletin of the Korean Chemical Society
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• v.7 no.4
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• pp.320-321
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• 1986

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.420-427
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• 2018

The orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (Sc YRS) and a variant of E. coli initiator tRNA, fMam $tRNA_{CUA}$ which recognizes the amber stop codon is an effective tool for site-specific incorporation of unnatural amino acids into the protein in E. coli. To evolve the amber suppression activity of the orthogonal pair, we generated a mutant library of Sc YRS by randomizing two amino acids at 320 and 321 which involve recognition of the first base of anticodon in fMam $tRNA_{CUA}$. Two positive clones are selected from the library screening with chloramphenicol resistance mediated by amber suppression. They showed growth resistance against high concentration of chloramphenicol and their $IC_{50}$ values were approximately 1.7~2.3 fold higher than the wild type YRS. In vivo amber suppression assay reveals that mutant YRS-3 (mYRS-3) clone containing amino acid substitutions of P320A and D321A showed 6.5-fold higher activity of amber suppression compared with the wild type. In addition, in vitro aminoacylation kinetics of mYRS-3 also showed approximately 7-fold higher activity than the wild type, and the enhancement was mainly due to the increase of tRNA binding affinity. These results demonstrate that optimization of anticodon recognition by engineered aminoacyl tRNA synthetase improves the efficiency of unnatural amino acid incorporation in response to nonsense codon.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.2
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• pp.321-323
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• 2012

Cognitive radio has been recently proposed to dynamically access unused-spectrum. Since the spectrum availability for opportunistic access is determined by spectrum sensing, sensing is identified as one of the most crucial issues of cognitive radio networks. The PHY-layer sensing, as a part of spectrum sensing in cognitive radio, concerns the sensing mechanism to determine channel to be sensed and to access. One of the important issues in the PHY-layer sensing control is to find an available sensing period and trade-off between spectrum sensing and data transmission. In this paper, we show the relationship between spectrum sensing and data transmission according to the sensing period. We analyze and propose the new scheme to evaluate optimal sensing period.

• Biomedical Science Letters
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• v.23 no.4
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• pp.321-326
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• 2017

Tomatoes are rich in nutrients and have many beneficial advantages on human health. Four cherry tomato supplementation diets (CTSDs) were prepared from the juice and cake of fresh and processed (heat-treated) cherry tomatoes. Rats were fed CTSDs for 28 days and the changes in health indices in the serum were analyzed. CTSDs significantly decreased (P < 0.05) food efficiency ratio compared with the control. CTSD feeding significantly increased (P < 0.05) the high-density lipoprotein cholesterol level compared with the control, which resulted in a significant (P < 0.05) decrease in coronary artery risk index and atherogenic index. Furthermore, CTSD feeding increased serum serotonin level. These results indicate that CTSD shows antihyperlipidemic effect.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.18 no.4
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• pp.321-326
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• 2005

Chemical mechanical polishing(CMP) process is essential technology to be applied to manufacturing the dielectric layer and metal line in semiconductor devices. It has been known that overpolishing in CMP depends on pattern selectivity as a function of density and pitch, and use of fixed abrasive pad(FAP) is one method which can improve the pattern selectivity. Thus, dishing & erosion defects can be reduced. This paper introduces the manufacturing technique of FAP using hydrophilic polymers with swelling characteristic in water and explains the self-conditioning phenomenon. When applied to tungsten blanket wafers, the FAP resulted in appropriate performance in point of uniformity, material selectivity and roughness. Especially, reduced dishing and erosion was observed in CMP of tungsten pattern wafer with the proposed FAP.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.38 no.6
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• pp.321-325
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• 2012

Collagen is widely used for regenerative therapy and pharmaceutical applications as one of the most useful scaffolds. Collagen is the most abundant protein in vertebrates and the natural substrate of various types of animal cells. Bone and dentin are mineralized tissues and almost similar in chemical components. They consist of collagen (18%), non-collagenous proteins (2%), hydroxyapatite (70%) and body fluid (10%) in weight volume. Pepsin-digested, type I collagen (atelocollagen) and heat-denatured collagen (gelatin) are basic collagenous materials for medical use. Demineralized dentin matrix (DDM) and demineralized bone matrix (DBM) belong to acid-insoluble group, and vital tooth-derived DDM is a unique dentin material including cementum and growth factors. In this review, collagen-based materials will be introduced and discussed for bone regenerative surgery.

• Communications for Statistical Applications and Methods
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• v.20 no.4
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• pp.321-327
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• 2013

Bayesian and empirical Bayesian methods have become quite popular in the theory and practice of statistics. However, the objective is to often produce an ensemble of parameter estimates as well as to produce the histogram of the estimates. For example, in insurance pricing, the accurate point estimates of risk for each group is necessary and also proper dispersion estimation should be considered. Well-known Bayes estimates (which is the posterior means under quadratic loss) are underdispersed as an estimate of the histogram of parameters. The adjustment of Bayes estimates to correct this problem is known as constrained Bayes estimators, which are matching the first two empirical moments. In this paper, we propose a way to apply the constrained Bayes estimators in insurance pricing, which is required to estimate accurately both location and dispersion. Also, the benefit of the constrained Bayes estimates will be discussed by analyzing real insurance accident data.

• Journal of Korean Institute of Industrial Engineers
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• v.40 no.3
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• pp.321-324
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• 2014

Kwon et al. (2013) studied the optimal monitoring interval of systems with finite life cycle. It is assumed that there are several failure modes from several failure causes and the occurrence of causes follows a homogeneous Poisson process. The total expected cost is used as an optimization criterion. In this article, we derive newly the total expected cost under the same assumptions and consider some extended models.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.3
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• pp.321-324
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• 1994

One hundred and twenty indigenous pigeons of either sexes of different age groups were sacrificed in different seasons to assess the interactions of sex, age and seasons on the proximate composition and gross energy values of fresh boneless meat. The dry matter and protein contents decreased but the fat, ash and energy contents increased linearly as the birds aged. Seasons and age had significant influences on either of these two performance traits. Meat of growing pigeons of both sexes was found to contain high protein and low fat throughout the year.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.2
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• pp.321-325
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• 2011

After investigating the analytic model to predict penetration depth, we propose an analytical model which can be used in estimating the normal penetration characteristics of small projectiles, when they are impacted to the concrete targets with ordnance velocities. The major parameters of this model are nose factor of penetrator, compressive strength and density of targets, and impact velocity. We can predict accelerations, velocities, displacements of projectiles and applied forces by this proposed model. Estimated penetration depths were shown 5% error. We also verified the usefulness of the new method with laboratory impact test data.