• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.103-108
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• 2002

We describe here the cDNA sequence and mRNA expression of a novel serine pretense from the firefly, Pyrocoelia rufa. The 771 bp cDNA encodes for 257 amino acid residues. The deduced protein of P. rufa serine pretense gene contains the catalytic triad and six-conserved cysteine residues. Alignment of the deduced protein of P. rufa serine pretense gene showed 47.4% protein sequence identity to known coleopteran insect Rhyzopertha dominica midgut trpsin-like enzyme. Northern blot analysis revealed that the P. rufa serine pretense is specifically expressed in the midgut of P. rufa larvae.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.57-62
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• 2001

A cDNA encoding the defensin homologue of a novel family member was isolated from the cDNA library of the firefly,Pyrocoelia rufa. Sequence analysis of the cDNA encoding the defensin homologue of P. rufa resulted that the 165 bp cDHA has an open reading frame of 55 amino acid residues. The deduced amino acid sequences of the defensin homologue gene from P. rufa showed identity to known mammalian defensins. Also 6 cystein residues in the P. rufa defensin homologue gene were conserved in the same position as those of known mammalian defensins. The result suggested that P. rufa defensin homologue is a novel member of the insect defensin family. Southern blot analysis suggests that there may be a single copy number of the P.rufa defensin homologue gene and their fat body-specific expression pattern at the transcriptional level was confirmed by Northern blot analysis.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.2
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• pp.101-107
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• 2002

We describe here the cDNA sequence and mRNA expression of a novel family of the antioxidant protein, peroxiredoxin, from the firefly, Pyracoetia ruin. The 555 bp cDNA sequence codes for a 185 amino acid protein with a calculated molecular mass of approximately 21 kDa. The deduced protein of P. rufa peroxiredoxin gene contains two conserved cysteine residues. Alignment of the deduced protein of P. rufa peroxiredoxin gene showed 71.1% protein sequenceidentity to known insect Drosophila melanogaster peroxiredoxin. Northern blot analysis revealed that the P. rufa peroxiredoxin is specifically expressed in the fat body of P. rufa larvae.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.181-189
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• 2003

We describe here the complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Pyrocoelia rufa. The luciferase gene of the P. rufa firefly consisted of six introns and seven exons coding for 548 amino acid residues. From the translational start site to the end of last exon, however, the genomic DNA length of the P. rufa luciferase gene from the Korean and Chinese samples spans 1,968 bp and 1983 bp, respectively, and 3 amino acid residues were different to each other. Additionally, we also analyzed mitochondrial cytochrome oxidase I(COI) gene of the Chinese P. rufa fireflies. Analysis of DNA sequences from the mitochondrial COI protein-coding gene revealed 4 mitochondrial DNA sequence-based haplotypes with a maximum divergence of 0.7％. With the 20 P. rufa haplotypes found in Korea, phylogenetic analyses using PAUP and PHYLIP subdivided the P. rufa into three clades, termed clades A and B for the Korean sample, and clade C for the Chinese sample.

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.2
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• pp.165-169
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• 2000

We have constructed cDNA library from the larvae whole body of the firefly, Pyrocoelia rufa. Single direct partial sequencing of anonymous cDNA clones was performed to obtain genetic information on the firefly, P. rufa, of which genetic information is currently not available. This expressed sequence tags (EST) analysis of the 54 clones (54%) showed significant homology to the known genes registered in GenBank. Of these clones, twenty-four were related to the known insect genes, but these clones were not matched to previously identified firefly genes. Putative functional categories of these clones showed that the next abundant genes were associated with energy metabolism.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.2
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• pp.167-172
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• 2001

The vitellin of firefly, Pyrocoelia rufa, is composed of three polypeptides, designated Vn1 (175 kDa), Vn2 (160 kDa) and Vn3 (45 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph, ovary and egg extracts, but not observed in the male. This vitellin was purified from the eggs of P. rufa by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In nature, vitellin of P. rufa has molecular weight of 400 kDa. Western blot analysis using polyclonal antiserum against purified vitellin showed that the antiserum was reacted with the three polypeptides, Vnl, Vn2 and Vn3 from the female adult hemolymph, ovary and egg extracts. Amino acid residues at N-terminus of three subunits were sequenced. The N-terminal sequences of large subunits, Vnl and Vn2, were similar to each other, But, the N-terminal sequences of small subunits Vn3, did not have any signnificant homology with large subunits.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.69-72
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• 2003

Development and life history of the Korean native firefly, Pyrocoelia rufa, were investigated throughout the indoor rearing. Average size of egg with an oval shape was 1.7 m and the hatchability of egg of P. rufa was approximately 88.5%. The larvae were pupated at the ${5^th}$ instar and the body length of the matured larvae was 27.1 mm. The total periods of larval stage to the end of the 5$^{th}$ instar were approximately 104.7 days. Average pupal period was 10.3 days and average number of eggs oviposited by a female was 87.3 eggs. The body size of female in the pupa and adult was larger than that of male. The wings of female adult were deteriorated.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.62-62
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• 2003

The Korean native firefly, P. rufa, is widely distributed in Korea and only at Tsushima in the case of Japan. P. rufa is known to occur in various habitats such as stream area, wet fields and mountain area. Development and life history of the Korean native firefly, Pyrocoelia rufa, were investigated throughout the indoor rearing. (omitted)

• Journal of Plant Biology
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• v.1 no.1
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• pp.11-16
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• 1958

한국산 Genus Vespula THOMSON 으로는 다음 6종이 알려지고 있다. Vespula austpiaca PANZER 삼성말벌 V. germanica flevicets SMITH 해첨배기말벌 V. media RETZIUS 좀말벌 V. koieensis YADOSZKOWSKI 참땅벌 V. rufa L. var. schrenckii RADOSZAKOWSKI 슈렝크말벌 V. vulgaris L. var. Lewisii CAMERON 땅벌 상기종중에서 V. austriaca, V. rufa, V. vulgaris의 종간은 각지에서 채집된 기록이 있으나 나머지 것은 한국에 산한다고만 되어있다. 채집된 상기 3종은 형태초적으로는 거의 비슷하고 반문 만을 달리하니 그 반문의 변이를 조사하는 것은 중대한 의의를 가진다. 필자는 서울 근교우이동에서 밤을 이용하여 8개의 벌집속 벌을 모조리 채집하여 그 중 2 벌집의 개체만에 관하여 그 변이를 조사한 결과를 제1보로 여기에 발표한다. 한 벌집 속 개체수는 726과 661 이었고 다라서 총개체수 1337 마리를 조사한 결과 상당히 심히 문반변이가 잇음을 알었고 더우기 V. austriaca, V. rufa, V. koreensis 와 V. vulgaris 간의 반문차는 동일종내의 변이에 불과함을 알게 되었다. 따라서, V. austriaca, V. rufa, V. koreensis는 V. vulgaris 의 synonym 임을 밝히는 바이다. 본연구에 있어서 귀중한 교\ulcorner을 빌려주신 고대이과부장 조복의교수와 재료의 채집과 조사에 협력해준 고대생물학과생 김원임, 이경\ulcorner, 이병준, 이동\ulcorner \ulcorner군에게 감사를 표하는 바이다.

• The Korean Journal of Mycology
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• v.27 no.5 s.92
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• pp.341-344
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• 1999

The genus Hypocrea, a member of the Hypocreales, has yellow to pale yellow-ochre, perithecial stromata and angular to cuboid ascospores. The isolate of Hypocrea microrufa collected on decayed tropical wood at Malaysia is compared with H. rufa and H. microsplendens. This isolation of H. microrufa will be the first record from tropical region and the first description with scanning electron microscopy. The ornamentation of ascospores used one of main taxonomic keys for identification.