• 한국수산과학회지
• /
• 제53권4호
• /
• pp.566-571
• /
• 2020

To classify a presumed hybrid of imported grouper species acquired from the National Fishery Products Quality Management Service, maternal and paternal lines were identified based on partial sequencing of mitochondrial cytochrome c oxidase 1 (co1) and nuclear recombination activation gene 1 (rag1) genes. The matrilineal species was identified as Epinephleus moara by a partial (760 bp) co1 sequence. Ambiguous sequences with base pairs belonging to E. moara or E. lanceolatus were found in a total of 15 different base pairs in the partial 1,159 bp of the rag1 gene, and the patrilineal species was found to be E. lanceolatus. Therefore, all of the groupers examined in the study were identified to be hybrids of E. moara and E. lanceolatus. In addition, a fast and convenient method using random amplification of polymorphic DNA (RAPD) was established for hybrid discrimination. Hybrids between E. moara ♀ and E. lanceolatus ♂ were identified through specific bands of 387 bp and 433 bp in PRIMER 6.

• 한국어류학회지
• /
• 제26권1호
• /
• pp.70-73
• /
• 2014

Interspecific hybrids between tiger grouper Epinephelus fuscoguttatus and giant grouper E. lanceolatus were genetically identified based on the partial sequence analysis of mitochondrial cytochrome c oxidase I (COX I) gene and nuclear recombination activating gene 2 (RAG 2) gene. Out of 585 base positions of RAG 2, a total of five nucleotide substitutions were detected between the two parental species (E. fuscoguttatus and E. lanceolatus). The hybrids had two distinct types of RAG 2 sequences corresponding to those of both parental species. Mitochondrial COX I gene sequencing showed that hybrids had sequences identical to E. fuscoguttatus. Molecular data clearly demonstrate that hybridization does occur between E. fuscoguttatus and E. lanceolatus, but with E. fuscoguttatus as the maternal parent.

• Fisheries and Aquatic Sciences
• /
• 제16권1호
• /
• pp.49-52
• /
• 2013

A single specimen of Epinephelus lanceolatus, belonging to Epinephelinae of the family Serranidae, was newly collected from Jeju Island, South Korea, in December 2009. Epinephelus lanceolatus is characterized by anterior lateral-line scales with branched tubules, cycloid scales, irregular white spots, and fins with numerous small black spots. We propose a Korean name, "Dae-wang-ba-ri' for this species.

• 한국어류학회지
• /
• 제27권3호
• /
• pp.199-204
• /
• 2015

묵납자루 Acheilognathus signifer와 납자루 A. lanceolatus간 잡종으로 추정되는 개체를 한강 수계의 김화남대천에서 채집하였다. 잡종의 기원을 확인하기 위하여 형태적 특징과 미토콘드리아 cytochrome b gene (cyt b)와 recombination-activating gene 1 (RAG-1)를 분석하였다. 외부 형태를 분석한 결과 잡종 개체는 등지느러미와 뒷지느러미 반문, 뒷지느러미 색깔, 몸의 체색 등은 두 부모종의 중간적 형질을 보였다. 미토콘드리아 cyt b 분석 결과 잡종 개체는 묵납자루와 염기서열이 99.9% 일치하여 묵납자루가 모계임이 밝혀졌다. 또한 RAG-1 분석 결과 double peak가 나타났는데 이는 잡종 개체임을 강력하게 시사하였다.

• Animal Systematics, Evolution and Diversity
• /
• 제18권2호
• /
• pp.203-211
• /
• 2002

한국의 동해안과 남해안에서 채집한 동문동물 1신종(Echinoderes lanceolatus)을 기재한다. 표본은 조하대의 저질과 해조류를 플랑크톤 넷트에 걸러 채집하였다. 이 종은 제3마디 흉판 중앙부 양측의 커다란 각흔(殼痕) 제4마디 흉판의 희미한 중앙연접 자국. 각각 해당 몸통 마디보다 약간 짧은 제6-10정중등가시. 암컷 제9-10마디 흉판의 중괄호형 근흔(筋痕), 마지막 복판의 뒷가장자리가 곧은점, 그리고 표창처럼 날카롭고 긴 제13마디의 등돌기가 특징이다.

• 한국수산과학회지
• /
• 제53권4호
• /
• pp.572-576
• /
• 2020

As grouper aquaculture production intensifies worldwide, hybrids are increasingly produced to induce heterosis of traits such as rapid growth and strong environmental adaptability. In this study, we compared the morphological characteristics of a hybrid grouper to those of its parent species Epinephelus moara and E. lanceolatus. The coefficients of external and skeletal traits evaluated using soft X-rays did not differ significantly among groups; however, several patterns were detected among measured traits. The body depth (BD), length of caudal peduncle (LCP), and pre-anal fin length (PAL) of the hybrid were the same as those of E. moara, whereas the pre-pectoral fin length (PPL) and interorbital width (IOW) of the hybrid were the same as those of E. lanceolatus. Traits that did not differ significantly among the three groups included pre-dorsal fin length (PDL), pre-ventral fin length (PVL), length of anal fin (LAF), and length of dorsal fin (LDF), whereas the depth of caudal peduncle (DCP) and snout length (SNL) showed intermediate traits, and eye diameter (ED) was significantly smaller in the hybrid. Therefore, intermediate traits such as DCP and SNL and specific traits such as ED may be used as indices for hybrid classification.

• 한국어류학회지
• /
• 제31권1호
• /
• pp.23-29
• /
• 2019

이번 연구에서 바리과 어종의 새로운 교잡종을 생산하기 위한 연구의 일환으로 능성어 (Hyporthodus septemfasciatus) ♀${\times}$대왕바리 (Epinephelus lanceolatus) ♂ (CGGG)의 발생 양상, 부화율, 기형 발생률 그리고 부화 소요시간을 조사하고, 교잡종의 모계어종의 순종 (능성어 ♀${\times}$♂, CG)과 비교하였다. CGGG와 CG 수정란은 자외선 살균 해수가 채워진 5-L 용량의 비이커 9개에 3,000~5,000개/비이커의 밀도로 수용되었으며, 발생 기간 중 수온은 $23.5{\sim}24.8^{\circ}C$ 그리고 염분은 32.1~32.8 ppt 였다. CGGG의 수정률과 부화율은 각각 $69.4{\pm}1.5%$와 $59.0{\pm}5.1%$로서 CG에 비해 유의하게 낮았다 (p<0.05). CGGG는 비균등 난할, 배체 체형 기형 그리고 부화자어의 척추 기형이 높게 출현한 것을 제외하고, CG와 동일한 난발생 양상을 보였다. CGGG의 부화까지 소요 시간은 CG에 비해 약 2시간 느렸다(p<0.05). 이상의 결과에서 CGGG는 수정률, 부화율 그리고 기형률 등 발생능력이 CG에 비해 낮았지만 부화자어의 대량생산이 가능할 것으로 판단된다.

• 한국수산과학회지
• /
• 제55권2호
• /
• pp.129-136
• /
• 2022

The nutrient composition of the muscle of the hybrid grouper (Epinephelus fuscoguttatus×E. lanceolatus, HG) and the hybrid longtooth grouper (E. moara×E. lanceolatus, HLG) was measured as a function of body weight in this study. The crude protein and lipid levels in HG were 21.0-21.2 g/100 g and 1.9-3.4 g/100 g, respectively. HLG contained 20.6-20.7 g/100 g protein, and 3.9-5.5 g/100 g crude lipid. The total amino acid contents of HG were 18,829.7-18,980.2 mg/100 g and that of HLG was 17,793.7-19,293.7 mg/100 g. The mean saturated fatty acid content was 0.61-1.09 g/100 g for HG and 1.27-1.77 g/100 g for HLG. Monounsaturated and polyunsaturated fatty acid levels were 0.59-1.02 g/100 g (Monoene), 0.67-1.11 g/100 g (Polyene) in HG, which were lower than the 1.21-1.78 (Monoene), 1.22-1.69 g/100 g (Polyene) found in HLG. The highest mineral content (K and P) of HG was 510.13-517.05 mg/100g and 231.59-247.67 mg/100 g, while that of HLG was 518.81-523.59 mg/100 g and 257.51-248.84 mg/100 g, respectively. In conclusion, there is potential for expanding the commercial utilization of HG and HLG as food resources as they are both nutrient-rich.

• Bulletin of the Korean Chemical Society
• /
• 제33권1호
• /
• pp.344-346
• /
• 2012

• 한국어병학회지
• /
• 제37권1호
• /
• pp.97-110
• /
• 2024

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.