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Embryonic Development of Fertilized Eggs of Convict Grouper (Hyporthodus septemfasciatus) ♀×Giant Grouper (Epinephelus lanceolatus) ♂  

Noh, Choong Hwan (Korea Institute of Ocean Science & Technology)
Yoon, Nak Jin (Cheong Sol Fisheries Co.)
Publication Information
Korean Journal of Ichthyology / v.31, no.1, 2019 , pp. 23-29 More about this Journal
Abstract
The embryonic development and hatchability of the artificially fertilized eggs of convict grouper (Hyporthodus septemfasciatus) ♀${\times}$giant grouper (Epinephelus lanceolatus) ♂ (CGGG) were compared to those of the maternal pure species (convict grouper ♀${\times}$♂, CG) to establish a novel grouper hybrid for aquaculture industry. The fertilized eggs were divided into nine 5-L beakers (3,000~5,000 eggs/beaker) filled with UV sterilized seawater and incubated at a temperature range of $23.5{\sim}24.8^{\circ}C$ (32.1~32.8 psu). The percentages of fertilization and hatching of CGGG were $69.4{\pm}1.5%$ and $59.0{\pm}5.1%$, respectively and were significantly lower than those of CG (p<0.05). The CGGG proceeded normal embryonic development similar to that of CG, but showed an irregular cleavage, immature embryonic body and spinal deformity in hatched larvae. The incubation time from fertilization to hatching of CGGG was 31 hrs, which was approximately 2 hrs slower than that of CG. Our study provided the possibility of mass production of grouper hybrid CGGG larvae.
Keywords
Hyporthodus septemfasciatus; Epinephelus lanceolatus; grouper hybrid; embryonic development; hatched larvae deformity;
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