Browse > Article
http://dx.doi.org/10.5657/KFAS.2020.0566

Molecular Biological Species Identification of Imported Groupers(Epinephelus moara ♀×E. lanceolatus ♂)  

Kim, Yong Hwi (Department of Life Science and Biotechnology, Soonchunhyang University)
Park, Jong Yeon (Department of Life Science and Biotechnology, Soonchunhyang University)
Kim, Jae Hoon (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Bang, In-Chul (Department of Life Science and Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.4, 2020 , pp. 566-571 More about this Journal
Abstract
To classify a presumed hybrid of imported grouper species acquired from the National Fishery Products Quality Management Service, maternal and paternal lines were identified based on partial sequencing of mitochondrial cytochrome c oxidase 1 (co1) and nuclear recombination activation gene 1 (rag1) genes. The matrilineal species was identified as Epinephleus moara by a partial (760 bp) co1 sequence. Ambiguous sequences with base pairs belonging to E. moara or E. lanceolatus were found in a total of 15 different base pairs in the partial 1,159 bp of the rag1 gene, and the patrilineal species was found to be E. lanceolatus. Therefore, all of the groupers examined in the study were identified to be hybrids of E. moara and E. lanceolatus. In addition, a fast and convenient method using random amplification of polymorphic DNA (RAPD) was established for hybrid discrimination. Hybrids between E. moara ♀ and E. lanceolatus ♂ were identified through specific bands of 387 bp and 433 bp in PRIMER 6.
Keywords
Epinephelus; co1; rag1; RAPD; Hybrid grouper;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Thompson JD, Higgins DG and Gibson TJ. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673.   DOI
2 Tian Y, Chen Z, Tang J, Duan H, Zhai J, Li B, Ma W, Liu J, Ho u Y and Sun Z. 2017. Effects of cryopreservation at various temperatures on the survival of kelp grouper (Epinephelus moara) embryos from fertilization with cryopreserved sperm. Cryobiology 75, 37-44. https://doi.org/10.1016/j.cryobiol.2017.02.007.   DOI
3 Wang S, Du J, Wang J and Ding S. 2007. Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers. Acta Oceanolog Sin 26, 122-129.
4 Bang KH, Chung JW, Kim YC, Cho IH, Kim JU, Shin MR and Moon JY. 2013. Analysis of genetic polymorphism of Korean ginseng cultivars and breeding lines using RAPD markers. J Korean Soc Int Agric 25, 184-193. https://doi.org/10.12719/KSIA.2013.25.2.184.   DOI
5 Choi JI. 2019. Molecular biological identification for two hybrid groupers, Epinephelus fuscoguttatus female$\times$E. lanceolatus male and E. akaara female$\times$E. lanceolatus male. MS Thesis, Soonchunhyang University, Asan, Korea.
6 Eschmeyer WN, Fricke R, Fong JD and Polack DA. 2010. Marine fish diversity: history of knowledge and discovery (Pisces). Zootaxa 2525, 19-50. https://doi.org/10.11646/zootaxa.2525.1.2.   DOI
7 FAO (Food and Agriculture Organization). 2017. FishStatJ, a tool for fishery statistics analysis. FAO Fisheries and Aquaculture Department, FIPS-Statistics and information. FAO, Rome, Italy.
8 Han SH, Kim MJ and Song CB. 2014. First record of the obliquebanded grouper, Epinephelus radiatus (Perciformes: Serranidae) from Korea. Korean J Ichthyol 26, 143-146.
9 Govindaraju GS and Jayasankar P. 2004. Taxonomic relationship among seven species of groupers (genus Epinephelus; family Serranidae) as revealed by RAPD fingerprinting. Mar Biotechnol 6, 229-237. https://doi.org/10.1007/s10126-003-0021-9.   DOI
10 Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95-98.
11 Hauswirth WW and Clayton DA. 1985. Length heterogeneity of a conserved displacement-loop sequence in human mitochondrial DNA. Nucleic Acids Res 13, 8093-8104. https://doi.org/10.1093/nar/13.22.8093.   DOI
12 Kim HS, Yun SW, Kim HT and Park JY. 2015. Occurrence of a Natural Hybrid between Acheilognathus signifer and A. lanceolatus (Pisces: Cyprinidae). Korean J Ichthyol 27, 199-204.
13 Kim KY and Bang IC. 2010. Molecular phylogenetic position of Abbottina springeri (Cypriniformes: Cyprinidae) based on nucleotide sequences of RAG1 gene. Korean J Ichthyol 22, 273-278.
14 Kim IS, Choi Y, Lee CL, Lee YJ, Kim BJ and Kim JH. 2005. Illustrated book of Korean fishes. Kyohak Publishing Co Ltd, Seoul, Korea, 613.
15 Kim KM, Lee HJ, Yun HB, Cho JH, Kim SR, Lee KM and Kim JH. 2020. Changes of hematological parameters and plasma components in the hybrid grouper (Epinephelus fuscoguttatus female$\times$E. lanceolatus male) by acute ammonia exposure. Korean J Environ Biol 38, 40-46. https://doi.org/10.11626/KJEB.2020.38.1.040.   DOI
16 Kim KS, Lee HR and Bang IC. 2014. Identification of Hybrid between the Tiger Grouper Epinephelus fuscoguttatus and the Giant Grouper E. lanceolatus by Analyzing COX I and RAG 2 Sequences. Korean J Ichthyol 26, 70-73.
17 Kim MJ and Song CB. 2010. First record of Epinephelus areolatus (Perciformes: Serranidae) from Korea. Korean J Fish Aquat Sci 13, 340-342. https://doi.org/10.5657/fas.2010.13.4.340
18 Kumar S, Stecher G, Li M, Knyaz C and Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35, 1547-1549. https://doi.org/10.1093/molbev/msy096.   DOI
19 Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111-120.   DOI
20 Kiriyakit A, Gallardo WG and Bart AN. 2011. Successful hybridization of groupers (Epinephelus coioides$\times$Epinephelus lanceolatus) using cryopreserved sperm. Aquaculture 320, 106-112. https://doi.org/10.1016/j.aquaculture.2011.05.012.   DOI
21 Park JM. 2018. A Study on the Hybrid between of Rhodeus uyekii and R. ocellatus. Ph. D Dissertation, Chonnam National University, Yeosu, Korea.
22 Lee IR, Yang H, Kim JH, Kim KY and Bang IC. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king Spine loach Iksookimia Longicorpa by Analyzing Mitochondrial COI and Nuclear RAG1 Sequences, Korean J Ichthyol 21, 287-290.
23 Lim SG, Han SB and Lim HK. 2016. Effects of salinity on the growth, survival and stress responses of red spotted grouper Epinesphelus akaara and hybrid grouper E. akaarafemale$\times$ E. lanceolatusmale. Korean J Fish Aquat Sci 49, 612-619. https://doi.org/10.5657/KFAS.2016.0612.   DOI
24 Myoung JG, Kang CB, Yoo JM, Lee EK, Kim S, Jeong CH and Kim BI. 2013. First record of the giant grouper Epinephelus lanceolatus (Perciformes: Serranidae: Epinephelinae) from Jeju island, South Korea. Korean J Fish Aquat Sci 16, 49-52. https://doi.org/10.5657/FAS.2013.0049.
25 Tahir D, Shariff M, Syukri F and Yusoff FM. 2018. Serum cortisol level and survival rate of juvenile Epinephelus fuscoguttatus following exposure to different salinities. Vet World 11, 327-331. https://doi.org/10.14202/vetworld.2018.327-331.   DOI
26 Pyo HJ and Choi KS. 1996. Analysis of genetic polymorphism among six Korean wild Artemisia spp. by using RAPD method. Korean J Agric Sci 23, 99-107.
27 Song HY and Bang IC. 2009. Genetic variation of Coreoleuciscus splendidus populations from four major rivers in Korea as assessed by RAPD PCR. Korean J Ichthyol 21, 129-133.
28 Song HY, Kim JH, Seo IY and Bang IC. 2017. Species and hybrid identification of genus Coreoleuciscus species in Hwnag-ji stream, Nakdong River Basin in Korea. Korean J Ichthyol 29, 1-12.
29 Tang J, Tian, Y, Li Z, Cheng M, Chen Z, Mao D and Li B. 2018. Analysis of genetic characters in Epinephelus moara, E. lanceolaus and their hybrids. J Agric Biotechnol 26, 819-829.