• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.566-571
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• 2020

To classify a presumed hybrid of imported grouper species acquired from the National Fishery Products Quality Management Service, maternal and paternal lines were identified based on partial sequencing of mitochondrial cytochrome c oxidase 1 (co1) and nuclear recombination activation gene 1 (rag1) genes. The matrilineal species was identified as Epinephleus moara by a partial (760 bp) co1 sequence. Ambiguous sequences with base pairs belonging to E. moara or E. lanceolatus were found in a total of 15 different base pairs in the partial 1,159 bp of the rag1 gene, and the patrilineal species was found to be E. lanceolatus. Therefore, all of the groupers examined in the study were identified to be hybrids of E. moara and E. lanceolatus. In addition, a fast and convenient method using random amplification of polymorphic DNA (RAPD) was established for hybrid discrimination. Hybrids between E. moara ♀ and E. lanceolatus ♂ were identified through specific bands of 387 bp and 433 bp in PRIMER 6.

• Korean Journal of Ichthyology
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• v.26 no.1
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• pp.70-73
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• 2014

Interspecific hybrids between tiger grouper Epinephelus fuscoguttatus and giant grouper E. lanceolatus were genetically identified based on the partial sequence analysis of mitochondrial cytochrome c oxidase I (COX I) gene and nuclear recombination activating gene 2 (RAG 2) gene. Out of 585 base positions of RAG 2, a total of five nucleotide substitutions were detected between the two parental species (E. fuscoguttatus and E. lanceolatus). The hybrids had two distinct types of RAG 2 sequences corresponding to those of both parental species. Mitochondrial COX I gene sequencing showed that hybrids had sequences identical to E. fuscoguttatus. Molecular data clearly demonstrate that hybridization does occur between E. fuscoguttatus and E. lanceolatus, but with E. fuscoguttatus as the maternal parent.

• Fisheries and Aquatic Sciences
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• v.16 no.1
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• pp.49-52
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• 2013

A single specimen of Epinephelus lanceolatus, belonging to Epinephelinae of the family Serranidae, was newly collected from Jeju Island, South Korea, in December 2009. Epinephelus lanceolatus is characterized by anterior lateral-line scales with branched tubules, cycloid scales, irregular white spots, and fins with numerous small black spots. We propose a Korean name, "Dae-wang-ba-ri' for this species.

• Korean Journal of Ichthyology
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• v.27 no.3
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• pp.199-204
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• 2015

A natural hybrid between Acheilognathus signifer and A. lanceolatus was collected in their sympatric area, the Gimhwanamdaecheon of Hangang (River), Korea. Morphological characters, mitochondrial cytochrome b gene (cyt b), and recombination-activating gene 1 (RAG-1) were investigated to confirm the natural hybrid origin. As a result of morphological characters, the natural hybrid was appeared to have intermediate characters between two parental species in three characters; the band of dorsal fin, the color patterns of anal fin membrane, and the body color. In analysis of cyt b, it was revealed that the maternal species of the natural hybrid was appeared to be A. signifer due to their 99.9% sequence identity. Also, in analysis of RAG-1, an electropherogram of the hybrid individual displayed double peaks, strongly indicating its hybrid state.

• Animal Systematics, Evolution and Diversity
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• v.18 no.2
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• pp.203-211
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• 2002

A new kinorhynch species belonging to the genus Echinoderes is described on the basis of the materials from the subtidal sediments and various macroalgae in the south and east coast of Korea. Echinoderes lanceolatus n. sp. is characteristic in having the subcuticular scars in subventral position on segment 3 and incomplete midventral articulation on segment 4, the middorsal spines a little shorter than the corresponding segments, the ‘brace-shaped’muscle scars at sternal plates of segments 9 and 10 in females, the flat posterior margin of terminal ventral plates and the lanceolate terminal tergal extension.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.572-576
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• 2020

As grouper aquaculture production intensifies worldwide, hybrids are increasingly produced to induce heterosis of traits such as rapid growth and strong environmental adaptability. In this study, we compared the morphological characteristics of a hybrid grouper to those of its parent species Epinephelus moara and E. lanceolatus. The coefficients of external and skeletal traits evaluated using soft X-rays did not differ significantly among groups; however, several patterns were detected among measured traits. The body depth (BD), length of caudal peduncle (LCP), and pre-anal fin length (PAL) of the hybrid were the same as those of E. moara, whereas the pre-pectoral fin length (PPL) and interorbital width (IOW) of the hybrid were the same as those of E. lanceolatus. Traits that did not differ significantly among the three groups included pre-dorsal fin length (PDL), pre-ventral fin length (PVL), length of anal fin (LAF), and length of dorsal fin (LDF), whereas the depth of caudal peduncle (DCP) and snout length (SNL) showed intermediate traits, and eye diameter (ED) was significantly smaller in the hybrid. Therefore, intermediate traits such as DCP and SNL and specific traits such as ED may be used as indices for hybrid classification.

• Korean Journal of Ichthyology
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• v.31 no.1
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• pp.23-29
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• 2019

The embryonic development and hatchability of the artificially fertilized eggs of convict grouper (Hyporthodus septemfasciatus) ♀${\times}$giant grouper (Epinephelus lanceolatus) ♂ (CGGG) were compared to those of the maternal pure species (convict grouper ♀${\times}$♂, CG) to establish a novel grouper hybrid for aquaculture industry. The fertilized eggs were divided into nine 5-L beakers (3,000~5,000 eggs/beaker) filled with UV sterilized seawater and incubated at a temperature range of $23.5{\sim}24.8^{\circ}C$ (32.1~32.8 psu). The percentages of fertilization and hatching of CGGG were $69.4{\pm}1.5%$ and $59.0{\pm}5.1%$, respectively and were significantly lower than those of CG (p<0.05). The CGGG proceeded normal embryonic development similar to that of CG, but showed an irregular cleavage, immature embryonic body and spinal deformity in hatched larvae. The incubation time from fertilization to hatching of CGGG was 31 hrs, which was approximately 2 hrs slower than that of CG. Our study provided the possibility of mass production of grouper hybrid CGGG larvae.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.2
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• pp.129-136
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• 2022

The nutrient composition of the muscle of the hybrid grouper (Epinephelus fuscoguttatus×E. lanceolatus, HG) and the hybrid longtooth grouper (E. moara×E. lanceolatus, HLG) was measured as a function of body weight in this study. The crude protein and lipid levels in HG were 21.0-21.2 g/100 g and 1.9-3.4 g/100 g, respectively. HLG contained 20.6-20.7 g/100 g protein, and 3.9-5.5 g/100 g crude lipid. The total amino acid contents of HG were 18,829.7-18,980.2 mg/100 g and that of HLG was 17,793.7-19,293.7 mg/100 g. The mean saturated fatty acid content was 0.61-1.09 g/100 g for HG and 1.27-1.77 g/100 g for HLG. Monounsaturated and polyunsaturated fatty acid levels were 0.59-1.02 g/100 g (Monoene), 0.67-1.11 g/100 g (Polyene) in HG, which were lower than the 1.21-1.78 (Monoene), 1.22-1.69 g/100 g (Polyene) found in HLG. The highest mineral content (K and P) of HG was 510.13-517.05 mg/100g and 231.59-247.67 mg/100 g, while that of HLG was 518.81-523.59 mg/100 g and 257.51-248.84 mg/100 g, respectively. In conclusion, there is potential for expanding the commercial utilization of HG and HLG as food resources as they are both nutrient-rich.

• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.344-346
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• 2012

• Journal of fish pathology
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• v.37 no.1
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• pp.97-110
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• 2024

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.