• Journal of Life Science
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• v.19 no.2
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• pp.277-283
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• 2009

Biogenic amines are generally formed through the decarboxylation of specific free amino acids by exogenous decarboxylases released by microbial species associated with the fish products and fermented feeds. This study was conducted to investigate the properties of e tuna waste regarding the control of degradation of biogenic amines (histamine, tyramine, tryptamine, putrescine, and cadaverine) that might be related with the anti-nutritional factor of the tuna waste that is used for manufacturing domestic fish meal. The values of pH and the salt content were 6.51, 3.35% in tuna waste and 5.58 and 5.83% in tuna fish meal, respectively. The strains and dominant bacteria tested in the tuna waste sample were 9.20, 9.29, 5.67, 7.82 and 7.58 log CFU/g of total bacteria, aerobic plate count (APC), total coliform (TC), Lactobacillus spp. and Bacillus spp., respectively. The main histamine forming-bacteria (HFB) in tuna waste were detected by silica gel thin-layer chromatography (TLC) and 7 histamine-forming bacterial species were isolated among microbes grown in selective medium. The histamine concentration was determined by detection of fluorescence of ο-phthaldialdehyde (OPA) derivatives using HPLC and the date were used to reconfirm the identities of the amine-producing bacteria. The 15 histamine- forming bacteria strains grown in trypicase soy broth (TSB) supplemented with 1% L-histidine (TSBH) were identified as Lactococcus(L.) lactis subsp. lactis, Klebsiella pneummonlae, L. garvieae 36, Vibrio olivaceus, Hafnia alvei and L. garvieae which were main dominant amine - producing strains, and Morganella morganii identified by 16S ribosomal RNA (rRNA) sequencing with PCR amplification. A Phylogenetic tree generated from the 16S rRNA sequencing data showed different phyletic lines that could be readily classified as biogenic amine forming gram-positive and negative bacteria.

• Korean Journal of Biological Psychiatry
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• v.9 no.1
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• pp.50-61
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• 2002

Objectives:High nailfold plexus visibility can reflect central nervous system defects as an etiologic factor of schizophrenia indirectly. Previous studies suggest that this visibility is particularly related to the negative symptoms of schizophrenia and frontal lobe deficiency. In this study, we examined the relationships between nailfold plexus visibility, and various clinical variables and neuropsychological functions in schizo-phrenic patients. Methods:Forty patients(21males, 19 females) satisfying the DSM-IV criteria for schizophrenia and thirty eight normal controls(20 males, 18 females) were measured for Plexus Visualization Score(PVS) by using the capillary microscopic examination. For the assessment of psychopathology, process-reactivity, premorbid adjustment, and neuropsychological functions, we used Positive and Negative Syndrome Scale(PANSS), Ullmann-Giovannoni Process-Reactive Questionnaire(PRQ), Phillips Premorbid Adjustment Scale(PAS), Korean Wechsler Adult Intelligence Scale(KWIS), Continuous Performance Test(CPT), Wisconsin Card Sort Test (WCST), and Word Fluency Test. We also collected data about clinical variables. Results:PVS was correlated with PANSS positive symptom score and composite score negatively. There were no correlations between PVS and PRQ score, PAS score and neuropsychological variables respectively. Conclusions:This study showed that nailfold plexus visibility was a characteristic feature in some schizophrenic patients, and that higher plexus visibility was associated with the negative symptoms of schizophrenia. There was no association between plexus visibility and neuropsychological functions.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.95-108
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• 1999

Field experiments were conducted in the 101 tobacco fields(51 fields in 1985 and 50 fields in 1986) of chief tobacco producing counties of Chungbuk province(Jincheon, Eumseong, Goesan, and Joongweon counties), Chungnam province(Cheonweon county), and Kyongbuk province (Cheongdo, Seongju, and Andong counties) for two years from 1985 to 1986 in order to evaluate soil fertility using chemical properties and soil map database. Pot experiments also on the same soils were conducted and the results were compared to those of field experiments. The yield of tobacco in the plots of no fertilization was considered as a basic factor representing the soil fertility and was evaluated by nineteen independent variables, that was 9 chemical properties and 10 soil map databases. These independent variables were classified into two groups, 11 quantitative indexes and 9 qualitative indexes, and were analyzed by multiple linear regression(MLR) of SAS by REG and GLM models. The yield of tobacco in the plot of no fertilization showed high variations, e.g. the difference between minimum and maximum yields was about 5.0-5.5 times in the pot experiment and 8.2-14.9 times in the field experiment. The indexes indicating close link between yield of tobacco and soil chemical indexes, was selected but it was not well matched by the years or between pot and field experiments. Also, the standardized partial regression coefficients of quantitative indexes for the yield of field were less than 1.0, suggesting that it is difficult to develop an available single index for the evaluation of soil fertility. Evaluation for the soil fertility of field by MLR was better than that of single regression and it was gradually improved by adding chemical properties, quantitative indexes, and qualitative indexes of soil map. For example, the coefficient of determination ($R^2$) of MLR for the yield of 1985 was increased to 0.422 with chemical indexes, 0.503 by addition of quantitative indexes, and 0.633 by the additional adding of qualitative indexes of soil map, compared to 0.244 of single index, $NO_3-N$ content of soil. Consequently, it is assumed that this approach by MLR with quantitative and qualitative indexes including chemical properties and soil map databases was available as an evaluation model of soil fertility for tobacco field.

• Journal of radiological science and technology
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• v.37 no.1
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• pp.49-56
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• 2014

Recently with CT developed, various studies for reduction of exposure dose is underway. Study of bismuth shields in these studies is actively underway, and has already been applied in the clinical. However, the application of the PET/CT examination was not activated. Therefore, through this study, depending on the application of bismuth shields in the PET/CT examination, we identify the quality of the image and the impact on the Standard Uptake Value (SUV). In this study, to apply to the shielding of the breast, by using the bismuth shields that contains 0.06 mm Pb ingredients, was applied to the PET/CT GEMINI TF 64 (Philips Healthcare, Cleveland, USA). Phantom experiments using the NEMA IEC Body Phantom, images were acquired according to the presence or absence of bismuth shields apply. Also, When applying, images were obtained by varying the spacing 0, 1, 2 cm each image set to the interest range in the depth of the phantom by using EBW-NM ver.1.0. When image of the PET Emission acquires, the SUV was in increased depending on the use of bismuth shields, difference in the depth to the surface from deep in the phantom increasingly SUV increased (P<0.005). Also, when using shields, as the more gab decreased, SUV is more increased (P<0.005). Through this study, PET/CT examination by using of bismuth shields which is used as purpose of reduction dose. When using shields, the difference of SUV resulting from the application of bismuth shields exist and that difference when gab is decrease and surface is wider. Therefore, setting spacing of shield should be considered, if considering the reduction of the variation of SUV and image quality, disease of deep organs should be a priority rather than superficial organ disease. Use of bismuth shielding factor considering the standard clinical examination, decrease unnecessary exposure can be expected to be considered.

• Journal of environmental and Sanitary engineering
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• v.20 no.1 s.55
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• pp.64-75
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• 2005

The total production of food waste was about 11,398ton/day('03) in Korea. Also, food waste was treated by landfill, incineration, reuse and anaerobic digestion. The method of food waste treatment depended primarily on landfill. However, the method of landfill causing social problems was prevented to treat food waste in the first of January 2005.12) Thus, anaerobic digestion is an important method to treat food waste because of possibility of energy recovery as methane gas. In this study, the possibility of food waste treatment containing high organic material and low pH in the one stage anaerobic reactor to save cost and time and energy recovery using $CH_{4}$ gas by the hybrid anaerobic reactor (HAR) was measured. The HAR was designed by combing the merits of the anaerobic filter (AF) to minimize the microorganism shock when food waste of very low pH was injected and up-flow anaerobic sludge blanket (UASB) to prevent from plugging and channeling phenomena by large suspended solids when semi solids were injected. Granule was packed in the section of HAR. The purpose of the BMP experiment was to measure the amount of methane generated when organic material was resolved under anaerobic conditions, to grasp bio resolution of organic material. Total accumulated methane production per VS amount was $0.471(m^{3}/\cal{kg}\;VS)$. So, the value was about $81.2\%$ of theoretical methane production which was $0.58(m^{3}/\cal{kg}\;VS)$ by elementary analysis and organic matter removal velocity (K) was $0.18(d^{-1})$. From these results, food waste was treated by anaerobic treatment. From this study, $CH_{4}$ generation from food waste (11,398 ton/day) could be estimated. By using an energy conversion factor of Braun's study, $5.97KWh/m^{3}\;CH4,\;60\%\;of\;CH_{4}$ gas generation, the amount of total energy producing food waste is to 6,727MWh/day. It could be confirmed that energy recovery using $CH_{4}$ gas was possible. Above these results, food waste containing organic matters of high concentration could be treated in HRT 30 days under an anaerobic condition, using the hybrid anaerobic reactor and reuse of $CH_{4}$ gas was possible.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.616-623
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• 2010

Plant samples from 49 sites for Chinese cabbage, 28 sites for radish, 16 sites for cabbage, 8 sites for head lettuce, 20 sites for onion from farmers' and experimental fields in highland of Korea were collected and analyzed to find out the uptake patterns of nitrogen (N), phosphorus (P) and potassium (K) by altitude. Dry weight and uptake of N, P and K were increased at higher altitude in most vegetable crops. Nutrition uptake by Chinese cabbage was 163 ~ 283 kg $ha^{-1}$ for N, 42 ~ 69 kg $ha^{-1}$ for $P_2O_5$ and 146 ~ 270 kg $ha^{-1}$ for $K_2O$ according to altitude. Nutrient uptake by radish according to altitude was 153~159 kg $ha^{-1}$ for N, 38 ~ 46 kg $ha^{-1}$ for $P_2O_5$, and 151 ~ 185 kg $ha^{-1}$ for $K_2O$. In case of cabbage, the plant uptakes of N, P, and K were increased at altitudes of 600 ~ 1,000 m. Nutrient uptake of cabbage was 280 ~ 348 kg $ha^{-1}$ for N, 34 ~ 87 kg $ha^{-1}$ for $P_2O_5$, and 209 ~ 290 kg $ha^{-1}$ for $K_2O$ according to altitude. Uptakes of N-$P_2O_5-K_2O$ by head lettuce at an altitude of 800 ~ 850 m were 93-26-126 kg $ha^{-1}$, respectively. Uptakes of N-$P_2O_5-K_2O$ by onions at an altitude of 600 ~ 800 m were 313-140-234 kg $ha^{-1}$, respectively, but there was no tendency in nutrition uptake patterns by altitude. Small cultivation areas used for leaf vegetable crops do not have fertilizer recommendation standards in alpine regions. It might be preferable to use a correction factor equivalent to the index of available nutrient uptake for the determination of N, P and K fertilizer application rates.

• Journal of Chest Surgery
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• v.38 no.10 s.255
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• pp.680-684
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• 2005

Background: With the increasing age of the population, coronary artery bypass grafting in the elderly patients is becoming common. Off-pump coronary artery bypass grafting (OPCAB) has been proven to be less morbidity and to facilitate early recovery. The elderly patients may have benefits by avoiding the adverse effects of the cardiopulmonary bypass. The purpose of this study is to evaluate our results of OPCAB in elderly patients. Material and Method: A retrospective chart review was carried out for 12 patients aged over 80 years who underwent isolated OPCAB from January 2001 and March 2004. Data were collected risk factors for disease, extent of coronary disease, and in-hospital outcomes. Postoperative graft patiency was evaluated in 9 patients by multi-slice computed tomography. Result: Eleven patients had triple vessel disease or left main disease. Four patients were suffered from preoperative CVA, and 4 patients had chronic obstructive pulmonary disease. Two patients had myocardial infarction (MI), among them 1 patient was suffered from pulmonary edema after preoperative MI. There was no perioperative death, perioperative MI, and no ventricular arrhythmia. Also there was no perioperative stroke and renal failure. But there was one deep sternal infection who recovered by treating of muscle flap. Atrial fibrillation was newly developed in 1 patient, but was well controlled by medication. Mean intubation time was $15.9\pm4.4(8\~20hrs)$ hrs and mean ICU stay was $2.9\pm0.8(2\~4 days)$ days. Mean hospital day was $21.6\pm14.3(13\~56 days)$ days. Postoperative mean CK-MS was $11.3\pm14.1\;ng/mL$. Early postoperative graft patency rate was $100\%(24/24)$. Follow-up was completed in all patients. In this time, there was no patients with angina or death. Conclusion: The results of this study suggest that OPCAB reduces morbidity and favors hospital outcomes. Therefore, OPCAB is safe, reasonable and might be preferable operative strategy in elderly patients.

• Journal of Chest Surgery
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• v.36 no.12
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• pp.921-927
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• 2003

Arterial switch operation (ASO) has been the most effective surgical option for transposition of the great arteries. But, the inappropriate dilation of the neoaortic root has been reported and its effect on neoaortic valve function and growth of aorta has not been well documented. Material and Method: Forty-eight patients who underwent cardiac catheterization during follow up after arterial switch operation were included in this study. Arterial switch operation was performed at a median age of 18 days (range 1∼211 days). Preoperative cardiac catheterization was performed in 26 patients and postoperative catheterization was performed in all patients at 15.8$\pm$9.6 months after ASO. Postoperative ratios of the diameters of neoaortic annulus, root and aortic anastomosis against the descending aorta were compared to the size of preoperative pulmonary annular, root and sinotubular junction. Preoperative and operative parameters were analyzed for the risk factors of neoaortic insufficiency. Result: There were two clinically significant neoaortic insufficiencies (grade$\geq$II/IV) during follow up, one of which required aortic valve replacement. Another patient required reoperation due to aortic stenosis on the anastomosis site. Post-operatively, neoaortic annulus/DA ratio increased from 1.33$\pm$0.28 to 1.52$\pm$.033 (p=0.01) and neoaortic root/DA ratio increased form 2.02$\pm$0.40 to 2.56$\pm$0.38 (p<0.0001). However, the aortic anastomosis/DA ratio showed no statistically significant difference (p=0.06). There was no statistically significant correlation between the occurrence of neoaortic insufficiency and neoaortic annulus/DA ratio and neoaortic root/DA ratio. Non-neonatal repair (age>30days) (p=0.02), preopeative native pulmonaic valve stenosis (p=0.01), and bisuspid pulmonic valve (p=0.03) were the risk factors for neoaortic insufficiency in univariate risk factor analysis. Conclusion: After ASO, aortic anastomosis site showed normal growth pattern proportional to the descending aorta, but neoaortic valve annulus and root were disproportionally dilated. Significant neoaortic valve insufficiency rarely developed after ASO and neoaortic annulus and root size do not correlate with the presence of postoperative neoarotic insufficiency. ASO after neonatal period, preoperative native pulmonary valve stenosis, and bicuspid native pulmonic valve are risk factors for the development of neoaortic insufficiency.

• Journal of Korea Water Resources Association
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• v.51 no.2
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• pp.141-150
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• 2018

The purpose of this study is to evaluate the future hydrologic behavior affected by the potential climate and land use changes in upstream of Anseong-cheon watershed ($366.5km^2$) using SWAT. The HadGEM3-RA RCP 4.5 and 8.5 scenarios were used for 2030s (2020-2039) and 2050s (2040-2059) periods as the future climate change scenario. It was shown that maximum changes of precipitation ranged from -5.7% in 2030s to +18.5% in 2050s for RCP 4.5 scenarios and the temperature increased up to $1.8^{\circ}C$ and $2.6^{\circ}C$ in 2030s RCP 4.5 and 2050s 8.5 scenarios respectively based on baseline (1976-2005) period. The future land uses were predicted using the CLUE-s model by establishing logistic regression equation. The 2050 urban area were predicted to increase of 58.6% (29.0 to $46.0km^2$). The SWAT was calibrated and verified using 14 years (2002-2015) of daily streamflow with 0.86 and 0.76 Nash-Sutcliffe model efficiency (NSE) for stream flow (Q) and low flow 1/Q respectively focusing on 2 drought years (2014-2015) calibration. For future climate change only, the stream discharge showed maximum decrease of 24.2% in 2030s RCP 4.5 and turned to maximum increase of 10.9% in 2050s RCP 4.5 scenario compared with the baseline period stream discharge of 601.0 mm by the precipitation variation and gradual temperature increase. While considering both future climate and land use change, the stream discharge showed maximum decrease of 14.9% in 2030s RCP 4.5 and maximum increase of 19.5% in 2050s RCP 4.5 scenario by the urban growth and the related land use changes. The results supported that the future land use factor might be considered especially for having high potential urban growth within a watershed in the future climate change assessment.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.1 no.1
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• pp.90-99
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• 1998

Purpose: The aim of this study was to clarify the serum cytokine pattern in patients with chronic HBV infection in terms of their clinical state. Methods: Intravenous blood samples were taken from 35 patients who were seropositive for HBsAg for at least 6 months and 7 healthy controls. Samples were initially tested for serum aminotransferases and serologic markers for hepatitis B virus by EIA. Serum levels of interleukin(IL)-2, tumor necrosis factor-alpha (TNF-${\alpha}$), interferon-gamma (IFN-${\gamma}$), IL-4, and IL-10 were measured by ELISA. Results: Among 35 patients, seropositive for HBeAg was 20 and for anti-HBe was 15. The histologic diagnosis of 19 patients underwent liver biopsy were chronic persistent hepatitis (CPH) in 10 and chronic active hepatitis (CAH) in 9. Serum IL-10 level in patients seropositive for HBeAg was significantly higher than that in patients seropositive for anti-HBe (p<0.05). All measured cytokine levels in patients with CAH were higher than those of patients with CPH. High values of all measured cytokines except IL-4 were seen in patients with AST and ALT > 100 U/L. High level of IL-4 was seen in patients with normal aminotransferase levels. Conclusion: These results were thought to indicate that anti-inflammatory Th2-like cytokine (IL-10) production in chronic HBV infection is related to circulating HBeAg rather than activity of hepatitis and that Th1 cytokines seem to be associated with the increasing activity of hepatitis.