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http://dx.doi.org/10.5352/JLS.2009.19.2.277

Characteristics of Histamine Forming Bacteria from Tuna Fish Waste in Korea  

Bang, Min-Woo (Department of Animal Science, Sunchon National University)
Chung, Chang-Dae (Department of Animal Science, Sunchon National University)
Kim, Seon-Ho (Department of Animal Science, Sunchon National University)
Chang, Moon-Baek (Department of Animal Science & Technology, Chung-Ang University)
Lee, Sung-Sil (Division of Applied Life Science, Gyengsang National University)
Lee, Sang-Suk (Department of Animal Science, Sunchon National University)
Publication Information
Journal of Life Science / v.19, no.2, 2009 , pp. 277-283 More about this Journal
Abstract
Biogenic amines are generally formed through the decarboxylation of specific free amino acids by exogenous decarboxylases released by microbial species associated with the fish products and fermented feeds. This study was conducted to investigate the properties of e tuna waste regarding the control of degradation of biogenic amines (histamine, tyramine, tryptamine, putrescine, and cadaverine) that might be related with the anti-nutritional factor of the tuna waste that is used for manufacturing domestic fish meal. The values of pH and the salt content were 6.51, 3.35% in tuna waste and 5.58 and 5.83% in tuna fish meal, respectively. The strains and dominant bacteria tested in the tuna waste sample were 9.20, 9.29, 5.67, 7.82 and 7.58 log CFU/g of total bacteria, aerobic plate count (APC), total coliform (TC), Lactobacillus spp. and Bacillus spp., respectively. The main histamine forming-bacteria (HFB) in tuna waste were detected by silica gel thin-layer chromatography (TLC) and 7 histamine-forming bacterial species were isolated among microbes grown in selective medium. The histamine concentration was determined by detection of fluorescence of ο-phthaldialdehyde (OPA) derivatives using HPLC and the date were used to reconfirm the identities of the amine-producing bacteria. The 15 histamine- forming bacteria strains grown in trypicase soy broth (TSB) supplemented with 1% L-histidine (TSBH) were identified as Lactococcus(L.) lactis subsp. lactis, Klebsiella pneummonlae, L. garvieae 36, Vibrio olivaceus, Hafnia alvei and L. garvieae which were main dominant amine - producing strains, and Morganella morganii identified by 16S ribosomal RNA (rRNA) sequencing with PCR amplification. A Phylogenetic tree generated from the 16S rRNA sequencing data showed different phyletic lines that could be readily classified as biogenic amine forming gram-positive and negative bacteria.
Keywords
Histamine forming bacteria; Biogenic amine; 16S rRNA; tuna fish waste;
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