• Title/Summary/Keyword: A culture society

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A Simple Culture Device for the Culture and Transportation of Mouse Oocytes and Embryos in vitro(I) (생쥐난자의 단기간 체외배양과 수송을 위한 Simple Culture Device)

  • Cho, Wan-Kyoo;Yoon, Yong-Dal
    • The Korean Journal of Zoology
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    • v.22 no.4
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    • pp.175-183
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    • 1979
  • This simple culture device is based on the microtube culture method (Cho, 1974). The microtube culture system was placed in a plastic tube (Falcon, 12 $\\times$ 75 mm) and the transporting system used in the present study were Trans-Temp 310 (Royal Industries, 209 D 135-1). The simple culture device in the Trans-Temp 310 seems to be an available method for short term culture and some distance transportation of oocytes and embryos.

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Studies on the Air-Liquid Interface Culture as an Experimental Model for Physiology and Pharmacology of Tracheal Epithelial Cells (기관(氣管) 상피세포 생리 및 약리 실험모델로서의 공기-액체 접면 일차배양법 연구)

  • 이충재;이재흔;석정호;허강민
    • Biomolecules & Therapeutics
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    • v.10 no.4
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    • pp.281-286
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    • 2002
  • In this study, we intended to get a preliminary data for establishing rat tracheal surface epithelial(RTSE) cell culture system as an experimental model for physiology and pharmacology of tracheal epithelial cells. Primary culture on the membrane support and application of the air-liquid interface system at the level of cell layer were performed. The cell growth rate and mucin production rate were measured according to the days in culture. The results were as follows: this culture system was found to manifest mucocilliary differentiation of rat tracheal epithelial cells, the cells were confluent and the quantity of produced and released mucin was highest on culture day 9, the mucin was mainly released to the apical side and tbe free $^3{H}$-glucosamine which was not incorporated to process of synthesis of mucin was left on the basolateral side. Taken together, we suggest that air-liquid interface culture system can be used as a substitute for immersion culture system and as an experimental model for in vivo mucus-hypersecretory diseases.

Factors Influencing Indonesian Consumers' Intention to Purchase Korean Food (인도네시아 소비자의 한국식품 구매의도 영향요인 분석)

  • Jeong, Jinyi;Choi, Young Min
    • Journal of the Korean Society of Food Culture
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    • v.34 no.5
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    • pp.543-552
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    • 2019
  • Indonesia has the largest economy in Southeast Asia and is one of the fastest growing economies of the world. This study was conducted to understand Indonesian food consumers, and to provide the marketing implications for food exporting companies in Korea. An online survey was conducted on 795 local consumers in Indonesia and the study analyzed their preference for Korean food and Korean culture. Then this study identified the relationship of these variables and consumers' intention to purchase Korean food. The results of this study were as follows. First, Indonesian consumers had a strong preference for Ramyeon, noodles, snacks and kimchi among Korean foods. Second, the preference for Korean culture was high in a descending order of Korean dramas, cosmetics, songs and news. Third, both Korean food and Korean culture preferences showed significant differences depending on gender, age and the frequency of eating out. Lastly, analysis of the relationships among the variables showed that there is a positive relationship between Korean food preferences and Korean food purchase intentions. Further, the consumers' Korean culture preference had a mediating effect within this relationship. The results of this study imply that marketers in Korea food companies need to consider the consumers' preference for Korean food and Korean culture when they build proper strategies for exporting Korean foods to Indonesia.

View sustainability of community identity and community culture - Centered on Dong-gye and Sangyeo-gye in Bufori, Yean-myeon (공동체 정체성과 공동체문화의 지속가능성 고찰 - 예안면 부포리의 동계와 상여계를 중심으로)

  • Lee, Mi Hong;Kwon, Ki Chang
    • Journal of Korea Multimedia Society
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    • v.24 no.7
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    • pp.942-952
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    • 2021
  • This study looked at the factors that shaped the identity of the Bupo-dong community through Dong-gye and Sangyeo-gye, mainly in the case of Bupori, Yeah, and Adong, and to identify that Dong-gye and Sangyeo-gye, played an important role in shaping and strengthening the community culture of its members. As a result of his inspection, Bupo-dong formed and maintained his belonging and identity as a member of Bupo-dong through the Dong-gye, the town's autonomy code. We were able to confirm that bupo-dong, unlike other villages where community culture is largely destroyed, has been able to continue its own community culture as a bupo people to this day, with a sense of belonging, solidarity and intimacy through the Sangyeo-gye of cooperation and relief. And it can be seen that the fact that the Burawon space, which has symbolic meaning to the people of Bupo-dong, exists without being watered down. It is important to discuss the community culture of Bupo-dong, which is sustainable, mainly in Bura-dong, which continues to be an important cultural space for bupo-dong people to meet and town events.

Comparison of Repast Tool Culture from Food Space of Korea.China.Japan (한.중.일 식공간에서의 취식문화(取食文化) 비교)

  • Lee, U-Joo
    • Journal of the Korean Society of Food Culture
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    • v.18 no.3
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    • pp.279-291
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    • 2003
  • Korea, China and Japan are countries located in Northeast Asia territory sharing similar natural environment. Countries are also using rice as a main diet material accompanied with vegetables, marine products and bean or fermented bean food. Yet, at the same time each country's food culture appeared as to be unique in food space due to their diverse food materials from different natural, cultural and religious background. This research is probing a way to recover subjective food culture and accomplish food environment which appropriates to modern globalized era by developing simple and economical repast tools meeting public's needs and distributing unique repast tools becomes Korean food culture and can be used in the life pattern of modernized nuclear families.

A Study on Positive Safety Reporting Culture in Aviation Maintenance (긍정적인 항공정비안전보고문화에 관한 연구)

  • Kim, Chun-Yong
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.20 no.2
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    • pp.64-71
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    • 2012
  • In field of Aviation Maintenance, honest and expedite voluntary report of potential hazard provide airworthiness aircraft by eliminating or avoiding from dangerous factors of aircraft. Although it supports for safety flight, voluntary incident reporting system consist of Aviation practitioner and require cooperation of practitioner due to there are no forcibleness. These occur when positive safety culture and report culture are settled. In this regard, this study firstly identify the current status of Aviation Safety Reporting System in Korea. Then, this article also find out the level of reporting culture of the AMT(Aircraft Maintenance Technicians) and problems in reporting system. Finally, suggestions on the model of positive safety reporting culture in a field of aircraft maintenance.

Korean Alcohol Beverage from the Viewpoint of Food Culture (한국(韓國) 술의 음식문화적(飮食文化的) 고찰(考察))

  • Koh, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.24 no.1
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    • pp.33-38
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    • 2009
  • Alcoholic beverages can be viewed as repositories of historical information reflecting agricultural environment, economic and scientific level, religion, literature and art of their country. Korean Alcoholic beverages are based largely on rice, which have been developed in the following order: Takju, Cheongju and distilled spirits, Soju. They are closely related with the development of agriculture and historical levels of science and technology, and thus can be seen as symbolizing economic and political changes as well as rises and declines throughout the culture's history. The present review assessed the past and present flows of Korean alcoholic beverage culture, which has had a significant influence on the mental world of Korean people, based on literature concerning the history of food in Korea. Another focus of the study was a discussion on the future transmission and refining of Korean alcoholic beverage culture, which is undeniably informed by the Korean people's unique imagination and cultural sense.

Perfusion Cultivation of Transgenic Nicotiana tabacum Suspensions in Bioreactor for Recombinant Protein Production

  • Lee Sang-Yoon;Kim Dong-Il
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.673-677
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    • 2006
  • A perfusion culture of transgenic Nicotiana tabacum cell suspensions, transformed to express recombinant glucuronidase (GUS), was successfully performed in a 5-1 stirred tank bioreactor. With 0.1 $day^{-1}$ of perfusion rate, the maximum dry cell weight (DCW) reached to 29.5 g/l in 16 days, which was 2.1-fold higher than the obtained in batch culture (14.3 g/l). In terms of the production of GUS, the volumetric activity could be increased up to 12.8 U/ml by using perfusion, compared with 4.9 U/ml in batch culture. The specific GUS activities in both perfusion and batch cultures were maintained at similar levels, 200-400 U/g DCW. Consequently, a perfusion culture could be a good strategy for the enhanced production of recombinant proteins in a plant cell culture system.

Development of the Pulsatile Pump System for a Perfusion Bioreactor (관류형 바이오리액터를 위한 박동 펌프 시스템 개발)

  • Kim, Hak-Jun;Kim, Sun-Hong;Chung, Ho-Yun;Yun, Won-Soo
    • Journal of the Korean Society for Precision Engineering
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    • v.28 no.4
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    • pp.526-533
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    • 2011
  • This research is about the pulsatile pump system utilized in the perfusion bioreactor for the in vitro human tissue culture. A pulsatile pump system which can be applied to the culture of the vascular tissues including blood vessel is developed by using the idea of human heart's blood pumping into organs as followings: culture chamber, a pressurizing device which generates laminar pulsatile flow by controlling the x-sectional area of the culture media delivering tubing, a compliance chamber which supplies the pressuring device with a constant pressure, and a peristaltic pump which circulates the culture media in a circuit ranging from the culture chamber to the compliance chamber. The developed pulsatile pump system shows that a physiology of the human heart's blood pumping including pulsatile pressure waveform of systolic-diastolic pressure is well represented. Not only time domain but also frequency domain characteristics of pulsatile pump system which are necessary for the vascular tissue culture such as pulsatile pressure waveform's shape, the frequency, and the magnitude can be easily generated and manipulated by using the proposed system.

Effect of Nursing Organizational culture, Organizational Silence, and Organizational Commitment on the Intention of Retention among Nurses: Applying the PROCESS Macro Model 6 (간호사의 재직의도에 대한 간호조직문화, 조직침묵과 조직몰입의 영향: PROCESS Macro model 6 적용)

  • Han, Sujeong
    • Korean Journal of Occupational Health Nursing
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    • v.31 no.1
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    • pp.31-41
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    • 2022
  • Purpose: This study aimed to identify the effects of organizational culture, organizational silence, and organizational commitment on the intention of retention as perceived by nurses Methods: The research model was designed on the basis of the PROCESS Macro model 6 proposed by Hayes. The participants were 142 nurses from general hospitals. Measurements included the scales of organizational culture, organizational silence, organizational commitment, and intention of retentione. Data were analyzed using descriptive statistics, Pearson's correlation coefficient analysis, and Hayes's PROCESS macro method for mediation. Results: Retention intention showed a significantly positive correlation with relationship-orientated culture (r=.32, p<.001), innovation-orientated culture (r=.30, p<.001), and organizational commitment (r=.48, p<.001). However retention intention showed a significantly negative correlation with organizational silence (r=-.42, p<.001). Furthermore, organizational silence and commitment had a mediating effect on the relationship between organizational culture and intention of retention. Conclusion: The impact of organizational culture on intention of retention in general hospitals was mediated by organizational silence and organizational commitment. Considering the mediating effects of organizational silence and organizational commitment on the relationship between nursing organizational culture and retention intention, a strategy should be developed to enhance organizational commitment and weaken organizational silence by strengthening related and innovative nursing culture.