• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권8호
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• pp.4191-4211
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• 2019

Software product lines (SPLs) are complex software systems by nature due to their common reference architecture and interdependencies. Therefore, any form of evolution can lead to a more complex situation than a single system. On the other hand, software product lines are developed keeping long-term perspectives in mind, which are expected to have a considerable lifespan and a long-term investment. SPL development organizations need to consider software evolution in a systematic way due to their complexity and size. Addressing new user requirements over time is one of the most crucial factors in the successful implementation SPL. Thus, the addition of new requirements or the rapid context change is common in SPL products. To cope with rapid change several researchers have discussed the evolution of software product lines. However, for the evolution of an SPL, the literature did not present a systematic process that would define activities in such a way that would lead to the rapid evolution of software. Our study aims to provide a requirements-driven process that speeds up the requirements engineering process using social network sites in order to achieve rapid software evolution. We used classification, topic modeling, and sentiment extraction to elicit user requirements. Lastly, we conducted a case study on the smartwatch domain to validate our proposed approach. Our results show that users' opinions can contain useful information which can be used by software SPL organizations to evolve their products. Furthermore, our investigation results demonstrate that machine learning algorithms have the capacity to identify relevant information automatically.

• 대한건축학회논문집:계획계
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• 제35권4호
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• pp.3-14
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• 2019

The purpose of this study is to analyze the distribution characteristics of the agency-managed shared housings in Seoul and draw the possible implications from it. The needed data for the study was obtained from an on-line shared housing portal service which provides housing information from various shared housing management agencies. By mapping out the locations and other related data into a GIS(Geographic Information System) program, this study shows that shared housings in Seoul can be sorted into four large clusters. Each cluster has a different ratio of housing types and room capacities reflecting each regional circumstance and common causes. Women-only shared housing takes up 79% of the overall shared housing ratio and, while multi-family housing type has the most count, the apartment type has a noticeably high ratio in women-only housing compared to the apartment type ratio among the other gender-specific housings. Given the high crime rate against the single-person household of young adult women, the reason for the popularity of shared-apartment housing among young women can be deduced. However, the potential tenants' practical choices and their concern for safety are not the only causes that drive the current shared housing market. The young adults in their 20's and 30's choose to live in popular places where they can engage social activities. A new tier of shared housing market has also appeared in places where young adults could not have afforded if they lived alone. Choosing where we live is no longer about just meeting the requirements for a house based on what she/he needs but also about satisfying the preferences for a living based on what she/he desires. This paper indicates that although there is an undeniable premise that 'sharing a house' revolves around the realm of housing welfare and is not a typical topic for the upper-income households, the way we live and how we choose our place to live is shifting.

• 한국정보통신학회논문지
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• 제10권1호
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• pp.117-122
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• 2006

본 논문에서는 MB-OFDM 방식의 초광대역 시스템 응용을 위한 단일 단 cascode 구조의 CMOS 저잡음증폭기를 설계하였다. 광대역 ($3.1GHz\~4.9GHz$) 입력 매칭은 칩 면적과 잡음지수를 줄이기 위해 간단한 대역 통과 필터를 사용하여 수행하였다. $0.18{\mu}m$ CMOS 공정변수를 사용하여 모의실험 한 결과, 설계된 증폭기는 9.7dB의 최대 이득, $2.1GHz\~7.1GHz$의 3dB 대역폭, 2dB의 최소잡음지수, -2dBm의 IIP3, -11.8dB 이하의 입력 반사 손실 특성을 보이며, 1.8V 공급 전원전압에 25.8mW의 전력을 소모한다. 칩면적은 패드를 포함해서 $0.74mm^2$이다.

• 대한전자공학회논문지SD
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• 제47권6호
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• pp.35-42
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• 2010

본 논문에서는 무선 통신 응용 시스템에 적합 하도록 10비트 80MHz 전류구동 방식의 D/A 변환기를 제안하였다. 제안한 회로는 $0.18{\mu}m$ CMOS n-well 1-poly 6-metal 공정을 이용하여 구현하였다. 10비트 중에서 LSB 4비트는 이진 디코더를 사용 하였으며, ULSB 3비트와 MSB 3비트는 온도계 디코더를 사용한 혼합구조를 채택하였다. 구현된 D/A 변환기의 측정결과, 샘플링 주파수가 80MHz, 입력 주파수 1MHz에서 SFDR은 60.42 dBc, 유효비트수는 8.75 비트를 보여주었다. INL/DNL은 ${\pm}$0.38LSB/${\pm}$0.32LSB로 측정되었으며, 글리치 에너지는 4.6 $pV{\cdot}s$로 나타났다. 전력 소모는 1.8V 전원전압에서 최대 속도인 80MHz일 때 48mW로 측정되었다.

• 한국정보통신학회논문지
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• 제6권4호
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• pp.519-526
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• 2002

본 논문에서는 이동 목표물의 속도를 측정하기 위한 도플러 레이더용 중심 주파수 10.525GHz인 마이크 로스트립 패치 배열 안테나를 설계 및 제작하였다. 이동용 레이더 시스템에 적용하기 위해서 비교적 충격에 강한 단일기판을 사용하였고, 원하는 특성을 얻기 위하여 급전회로망은 corporated 3-dB amplitude taper를 이용하여 4 $\times$ 8로 배열하였다. 또한 정사각형의 패치 소자를 사용하여 원편파를 이용할 수 있도록 하였다. 설계 및 제작된 안테나는 반사계수(S$_1$$_1$) -53.498dB, 수평 빔폭 $10^{\circ}$, 수직 빔폭 $18.8^{\circ}$, 이득 21dBi 및 SLL(Side Lobe Level) -17.5 dB 이하, 220MHz의 VSMR 1.5 이하 대역폭을 얻었다.

• 한국도서관정보학회지
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• 제22권
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• pp.205-244
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• 1995

Originally the concept of the traditional resource sharing has been discussed in the context of bibliographic materials, and has been labor-intensive and high-cost activities. The technology has had a great impact on such pattern of the resource sharing, and has expanded the limited scope of the traditional concept into the sharing of library information in the levels of local, regional and national systems, and expertise, materials, facilities, equipments and personnels of the library system. While the traditional circulation service as a basic method to share library materials by users can provide the resource to a single person at a time, the electronic resource can be shared, by multi-users at a time anytime anywhere. The maximization of the electronic resource sharing requires that publishing process should be fundamentally changed and articles, books, chapters, speech manuscripts, music scores, maps, sound, and other formats of materials should be prepared in machine readable format. This study examined the positive effects of the technology on the resource sharing, but not investigate the concrete and complex problems as to the cost, guidelines, detailed procedures, design details, and intellectual properties and protection involved in the resource sharing. Some findings extracted from the study can be summarized as follows; (1) ILL will lose its meaning as a method to share the materials if they are all in the electronic format and the phrase 'networked information resource' becomes omnipresent. (2) The technology keeps on changing the concept of resource sharing. Today, the scope of resource sharing not only encompasses the sharing of the primary and secondary materials but also the sharing of the processings(eg. cataloging), expertise, user education, special facilities, and the integrated automated library systems. (3) The sharing of the networked resource will be a method to provide better services for library users in the low cost. (4) The a n.0, pplication of the technology to the resource sharing should be focus on the method which allows an end-users to do the direct access to the needed materials, and to be delivered the primary document as soon as possible.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.69-73
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• 2013

Background: An increasing trend of cytotoxic drug use, mainly in cancer treatment, has increased the occupational exposure among the nurses. This study aimed to assess the change of nurses' safety-related knowledge as well as attitude levels and subsequently to assess the change of cytotoxic drug handling practices in wards after a series of pharmacist-based interventions. Materials and Methods: This prospective interventional study with a before and after design requested a single group of 96 nurses in 15 wards actively providing chemotherapy to answer a self-administered questionnaire. A performance checklist was then used to determine the compliance of all these wards with the recommended safety measures. The first and second assessments took 2 months respectively with a 9-month intervention period. Pharmacist-based interventions included a series of technical, educational and administrative support measures consisting of the initiation of closed-system cytotoxic drug reconstitution (CDR) services, courses, training workshops and guideline updates. Results: The mean age of nurses was $32.2{\pm}6.19$ years. Most of them were female (93.8%) and married (72.9%). The mean knowledge score of nurses was significantly increased from $45.5{\pm}10.52$ to $73.4{\pm}8.88$ out of 100 (p<0.001) at the end of the second assessment. Overall, the mean practice score among the wards was improved from $7.6{\pm}5.51$ to $15.3{\pm}2.55$ out of 20 (p<0.001). Conclusions: The pharmacist-based interventions improved the knowledge, attitude and safe practices of nurses in cytotoxic drug handling. Further assessment may help to confirm the sustainability of the improved practices.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제22권4호
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• pp.7-17
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• 2016

Purpose: As our population ages and becomes an elderly society the number of elderly care hospitals is rapidly increasing. Because physical functions and spatial perception in the elderly decrease with age, these hospitals require more systematic and intelligent space designs. The design of these spaces are even more complex because they must accommodate medical programs to treat various different diseases and ailments and also because there are many first time patients and irregular short term patients that seek out outpatient treatment services. Also by analyzing the spatial configuration systems and systematic relationships between each of the functional spaces of the outpatient treatment service departments for hospitals specialized in care for the elderly by focusing on the hallway and corridor systems of these hospitals, the according characteristics and trends were examined. Methods: Based on preceding research, the types of hallway and corridor systems of these hospitals were categorized into five types, including gallery corridors, middle corridors, hall-type, mixed type and cyclic type corridors, and into six types according to function including by medical diagnosis, patient registration, examination, administration and convenience and shared common space to derive any interconnecting relationships between the corridor systems. Also by comprehensively examining the types and combined utilization of the corridor types and the integration and the intelligibility of the space syntax, any trends within the corridor system were derived. The elderly care hospitals examined in this research study were twelve hospitals that opened after the year 2000 in Korea with more that 150 sick beds with areas larger than $1000m^2$ and with all outpatient medical service related rooms located entirely on a single floor of the hospital. Results: The following results could be confirmed based on this research study. 1) The spaces where medical diagnosis and examination occurred were adjacent, and the movement lines for first time patients and re-visiting patients were taken into consideration by separating the treatment space. 2) This research study confirmed that the larger the size of the hospital was, there were more detailed categorizations of treatment services and that there was a tendency for treatment areas to be separated and independent from examination areas. 3) There was a tendency for integration and intelligibility to decrease the more complex and diverse the combination of hall types designed into the corridor systems of these hospitals was. cyclic type corridors dramatically decreased the intelligibility of the corridor systems of these hospitals. 4) The priority rank of these spaces were confirmed to be highest in the order of registration, diagnosis, examination, treatment, administration and shared common spaces. However it was confirmed for the local integration that the diagnosis scope had the highest priority rank. Implications: There were exceptional cases confirmed where the number of unit spaces did not have an absolute effect on integration and intelligibility. These results can be interpreted to mean that this can be overcome through efficient architectural planning.

• 한국정보과학회논문지:시스템및이론
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• 제27권2호
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• pp.177-187
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• 2000

최근 몇 년간 클리스터링 기반 다중 프로세서 시스템에서의 상호 연결망으로서의 버스의 제약을 극복하기 위한 단방향 지점간 링크를 이용한 링 구조가 제안되었다. 하지만 계속되는 프로세서의 고속화와 지역 버스 및 메모리의 고성능화로 인해 지점간 링크의 현재 표준 대역폭으로는 시스템 확장성에 한계를 보이며 이에 따라 대역폭 확장에 대한 연구가 필요하다. 본 논문에서는 클리스터링 기반 다중프로세서 시스템으로 개발된 PANDA 시스템을 기본 모델로 채택한다. 최근 대중화된 프로세서 및 지역 버스의 사양을 반영한 모의실험을 통해 현재의 지점간 링크가 전체 시스템 성능에 병목이 됨을 보여주고 두 배 이상의 대역폭 확장이 필요함을 보인다. 상호 연결망의 대역폭을 확장하기 위해, 두 배 증가된 대역폭을 지닌 새로운 링크를 개발하는 것은 과다한 설계비용과 개발시간이 요구된다. 이에 대한 대안으로 본 논문에서는 상용화되어있어 쉽게 적용 가능한 기존 IEEE 표준 대역폭을 가진 링크를 이용해 이중으로 링을 구성하는 몇 가지 방법 단순 이중 링, 트랜잭션 분리 이중 링, 방향 분리 이중 링 - 을 제시하고 모의실험을 통해 두 배 대역폭 단일 링과 더불어 각각의 방식에 대한 장단점을 분석한다.