• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.8
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• pp.4191-4211
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• 2019

Software product lines (SPLs) are complex software systems by nature due to their common reference architecture and interdependencies. Therefore, any form of evolution can lead to a more complex situation than a single system. On the other hand, software product lines are developed keeping long-term perspectives in mind, which are expected to have a considerable lifespan and a long-term investment. SPL development organizations need to consider software evolution in a systematic way due to their complexity and size. Addressing new user requirements over time is one of the most crucial factors in the successful implementation SPL. Thus, the addition of new requirements or the rapid context change is common in SPL products. To cope with rapid change several researchers have discussed the evolution of software product lines. However, for the evolution of an SPL, the literature did not present a systematic process that would define activities in such a way that would lead to the rapid evolution of software. Our study aims to provide a requirements-driven process that speeds up the requirements engineering process using social network sites in order to achieve rapid software evolution. We used classification, topic modeling, and sentiment extraction to elicit user requirements. Lastly, we conducted a case study on the smartwatch domain to validate our proposed approach. Our results show that users' opinions can contain useful information which can be used by software SPL organizations to evolve their products. Furthermore, our investigation results demonstrate that machine learning algorithms have the capacity to identify relevant information automatically.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.4
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• pp.3-14
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• 2019

The purpose of this study is to analyze the distribution characteristics of the agency-managed shared housings in Seoul and draw the possible implications from it. The needed data for the study was obtained from an on-line shared housing portal service which provides housing information from various shared housing management agencies. By mapping out the locations and other related data into a GIS(Geographic Information System) program, this study shows that shared housings in Seoul can be sorted into four large clusters. Each cluster has a different ratio of housing types and room capacities reflecting each regional circumstance and common causes. Women-only shared housing takes up 79% of the overall shared housing ratio and, while multi-family housing type has the most count, the apartment type has a noticeably high ratio in women-only housing compared to the apartment type ratio among the other gender-specific housings. Given the high crime rate against the single-person household of young adult women, the reason for the popularity of shared-apartment housing among young women can be deduced. However, the potential tenants' practical choices and their concern for safety are not the only causes that drive the current shared housing market. The young adults in their 20's and 30's choose to live in popular places where they can engage social activities. A new tier of shared housing market has also appeared in places where young adults could not have afforded if they lived alone. Choosing where we live is no longer about just meeting the requirements for a house based on what she/he needs but also about satisfying the preferences for a living based on what she/he desires. This paper indicates that although there is an undeniable premise that 'sharing a house' revolves around the realm of housing welfare and is not a typical topic for the upper-income households, the way we live and how we choose our place to live is shifting.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.1
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• pp.117-122
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• 2006

A CMOS LNA based on a single-stage cascode configuration is designed for MB-OFDM ultra-wide band(UWB) systems. Wideband($3.1GHz\~4.9GHz$) input matching is performed using a simple bandpass filter to minimize the chip size and the noise figure degradation. The simulation results using $0.18{\mu}m$ CMOS process parameters show a power gain of 9.7dB, a 3dB band width of $2.1GHz\~7.1GHz$, a minimum NF of 2dB, an IIP3 of -2dBm. better than -11.8dB of input matching while occupying only $0.74mm^2$ of chip area. It consumes 25.8mW from a 1.8V supply.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.6
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• pp.35-42
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• 2010

This paper describes a 10 bit 80MHz CMOS D/A converter for wireless communication system. The proposed circuit in the paper is implemented with a $0.18{\mu}m$ CMOS n-well 1-poly 6-metal process. The architecture of the circuit consists of the 4bit LSB with binary decoder, and both the 3bit ULSB and the 3bit MSB with the thermometer decoder. The measurement results demonstrates SFDR of 60.42dBc at sampling frequency 80MHz, input frequency 1MHz and ENOB of 8.75bit. INL and DNL have been measured to be ${\pm}$0.38LSB and ${\pm}$0.32LSB and glitch energy is measured to be 4.6$pV{\cdot}s$. Total power dissipation is 48mW at 80MHz(maximum sampling frequency) with a single power supply of 1.8V.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.4
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• pp.519-526
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• 2002

In this paper, a microstrip patch array antenna for a Doppler radar at 10.525GHz is desinged and fabricated. To be used for mobile radar system, the antenna is fabricated on a single layer laminate to resist a fire impact and is covered with the Teflon foam. To obtain the desired characteristics, the array antenna is designed 4$\times$8 array using a corporate 3-dB amplitude taper. Also, using square patch elements, the antenna can be converted to a circular polarized antenna later. The designed and fabricated array antenna has the reflection coefficient$({S_11})$ -53.498dB, the horizontal beam width of $10^{\circ}$, the vertical beam width of $18.8^{\circ}$, the gain of 21dBi, the bandwidth of 220MHz for VSWR<1.5 and a side lobe level of less than -17.5dB.

• Journal of Korean Library and Information Science Society
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• v.22
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• pp.205-244
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• 1995

Originally the concept of the traditional resource sharing has been discussed in the context of bibliographic materials, and has been labor-intensive and high-cost activities. The technology has had a great impact on such pattern of the resource sharing, and has expanded the limited scope of the traditional concept into the sharing of library information in the levels of local, regional and national systems, and expertise, materials, facilities, equipments and personnels of the library system. While the traditional circulation service as a basic method to share library materials by users can provide the resource to a single person at a time, the electronic resource can be shared, by multi-users at a time anytime anywhere. The maximization of the electronic resource sharing requires that publishing process should be fundamentally changed and articles, books, chapters, speech manuscripts, music scores, maps, sound, and other formats of materials should be prepared in machine readable format. This study examined the positive effects of the technology on the resource sharing, but not investigate the concrete and complex problems as to the cost, guidelines, detailed procedures, design details, and intellectual properties and protection involved in the resource sharing. Some findings extracted from the study can be summarized as follows; (1) ILL will lose its meaning as a method to share the materials if they are all in the electronic format and the phrase 'networked information resource' becomes omnipresent. (2) The technology keeps on changing the concept of resource sharing. Today, the scope of resource sharing not only encompasses the sharing of the primary and secondary materials but also the sharing of the processings(eg. cataloging), expertise, user education, special facilities, and the integrated automated library systems. (3) The sharing of the networked resource will be a method to provide better services for library users in the low cost. (4) The a n.0, pplication of the technology to the resource sharing should be focus on the method which allows an end-users to do the direct access to the needed materials, and to be delivered the primary document as soon as possible.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.69-73
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• 2013

Background: An increasing trend of cytotoxic drug use, mainly in cancer treatment, has increased the occupational exposure among the nurses. This study aimed to assess the change of nurses' safety-related knowledge as well as attitude levels and subsequently to assess the change of cytotoxic drug handling practices in wards after a series of pharmacist-based interventions. Materials and Methods: This prospective interventional study with a before and after design requested a single group of 96 nurses in 15 wards actively providing chemotherapy to answer a self-administered questionnaire. A performance checklist was then used to determine the compliance of all these wards with the recommended safety measures. The first and second assessments took 2 months respectively with a 9-month intervention period. Pharmacist-based interventions included a series of technical, educational and administrative support measures consisting of the initiation of closed-system cytotoxic drug reconstitution (CDR) services, courses, training workshops and guideline updates. Results: The mean age of nurses was $32.2{\pm}6.19$ years. Most of them were female (93.8%) and married (72.9%). The mean knowledge score of nurses was significantly increased from $45.5{\pm}10.52$ to $73.4{\pm}8.88$ out of 100 (p<0.001) at the end of the second assessment. Overall, the mean practice score among the wards was improved from $7.6{\pm}5.51$ to $15.3{\pm}2.55$ out of 20 (p<0.001). Conclusions: The pharmacist-based interventions improved the knowledge, attitude and safe practices of nurses in cytotoxic drug handling. Further assessment may help to confirm the sustainability of the improved practices.

• Journal of The Korea Institute of Healthcare Architecture
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• v.22 no.4
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• pp.7-17
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• 2016

Purpose: As our population ages and becomes an elderly society the number of elderly care hospitals is rapidly increasing. Because physical functions and spatial perception in the elderly decrease with age, these hospitals require more systematic and intelligent space designs. The design of these spaces are even more complex because they must accommodate medical programs to treat various different diseases and ailments and also because there are many first time patients and irregular short term patients that seek out outpatient treatment services. Also by analyzing the spatial configuration systems and systematic relationships between each of the functional spaces of the outpatient treatment service departments for hospitals specialized in care for the elderly by focusing on the hallway and corridor systems of these hospitals, the according characteristics and trends were examined. Methods: Based on preceding research, the types of hallway and corridor systems of these hospitals were categorized into five types, including gallery corridors, middle corridors, hall-type, mixed type and cyclic type corridors, and into six types according to function including by medical diagnosis, patient registration, examination, administration and convenience and shared common space to derive any interconnecting relationships between the corridor systems. Also by comprehensively examining the types and combined utilization of the corridor types and the integration and the intelligibility of the space syntax, any trends within the corridor system were derived. The elderly care hospitals examined in this research study were twelve hospitals that opened after the year 2000 in Korea with more that 150 sick beds with areas larger than $1000m^2$ and with all outpatient medical service related rooms located entirely on a single floor of the hospital. Results: The following results could be confirmed based on this research study. 1) The spaces where medical diagnosis and examination occurred were adjacent, and the movement lines for first time patients and re-visiting patients were taken into consideration by separating the treatment space. 2) This research study confirmed that the larger the size of the hospital was, there were more detailed categorizations of treatment services and that there was a tendency for treatment areas to be separated and independent from examination areas. 3) There was a tendency for integration and intelligibility to decrease the more complex and diverse the combination of hall types designed into the corridor systems of these hospitals was. cyclic type corridors dramatically decreased the intelligibility of the corridor systems of these hospitals. 4) The priority rank of these spaces were confirmed to be highest in the order of registration, diagnosis, examination, treatment, administration and shared common spaces. However it was confirmed for the local integration that the diagnosis scope had the highest priority rank. Implications: There were exceptional cases confirmed where the number of unit spaces did not have an absolute effect on integration and intelligibility. These results can be interpreted to mean that this can be overcome through efficient architectural planning.

• Journal of KIISE:Computer Systems and Theory
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• v.27 no.2
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• pp.177-187
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• 2000

In the past several years, many systems which adopted ring topology with high-speed unidirectional point-to-point links have emerged to overcome the limit of bus for interconnection network of clustered multiprocessor system. However, rapid increase of processor speed and performance improvement of local bus and memory system limit scalability of system with point-to-point link of standard bandwidth. Therefore, necessity to extend bandwidth is emphasized. In this paper, we adopt PANDA system as base model, which is clustering-based multiprocessor system. By simulating a model adopting commercial processor and local bus specification, we show that point-to-point link is bottleneck of system performance, and bandwidth expansion by more than 200% is needed. To expand bandwidth of interconnection network, it needs excessive design cost and time to develop new point-to-point link with doubled bandwidth. As an alternative to double bandwidth, we propose several ways to implement dual ring -simple dual ring, transaction-separated dual ring, direction-separated dual ring- by using off-the-shelf point-to-point links with IEEE standard bandwidth. We analyze pros. and cons. of each model compared with doubled-bandwidth single ring by simulation.