• 제목/요약/키워드: 97B99

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Molecular Characterization and Phylogenetic Analysis of Season Influenza Virus Isolated in Busan during the 2006-2008 Seasons (부산지역에서 유행한 계절인플루엔자바이러스의 유전자 특성 및 계통분석('06-'08 절기))

  • Park, Yon-Koung;Kim, Nam-Ho;Choi, Seung-Hwa;Lee, Mi-Oak;Min, Sang-Kee;Kim, Seong-Joon;Cho, Kyung-Soon;Na, Young-Nan
    • Journal of Life Science
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    • v.20 no.3
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    • pp.365-373
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    • 2010
  • To monitor newly emerged influenza virus variants and to investigate the prevalence pattern, our laboratory performed isolation of the viruses from surveillance sentinel hospitals. In the present study, we analysed influenza A/H1N1, A/H3N2, B viruses isolated in Busan during the 2006/07 and 2007/08 seasons by sequence analysis of the hemagglutinin (HA1 subunit) and neuraminidase (NA) genes. The isolates studied here were selected by the stratified random sample method from a total of 277 isolates, in which 15 were A/H1N1, 16 were A/H3N2 and 29 were B. Based on the phylogenetic tree, the HA1 gene showed that A/H1N1 isolates had a 96.7% to 97.7% homology with the A/Brisbane/59/2007, A/H3N2 isolates had a 98.4% to 99.7% homology with the A/Brisbane/10/2007, and B isolates had a 96.5% to 99.7% homology with the B/Florida/4/2006(Yamagata lineage), which are all the vaccine strains for the Northern Hemisphere in 2008~2009 season. In the case of the NA gene, A/H1N1 isolates had 97.8% to 98.5% homologies, A/H3N2 isolates had 98.9% to 99.4% homologies, and B isolates had 98.9% to 100% homologies with each vaccine strain in the 2008~2009 season, respectively. Characterization of the hemagglutinin gene revealed that amino acids at the receptor-binding site and N-linked glycosylation site were highly conserved. These results provide useful information for the control of influenza viruses in Busan and for a better understanding of vaccine strain selection.

A Study on the Characteristics of Color trend for Interior textile (인테리어 직물색채의 트랜드변화 특성에 관한 연구)

  • 박소영;박영순
    • Korean Institute of Interior Design Journal
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    • no.23
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    • pp.109-115
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    • 2000
  • The purpose of this study is to examine the interior textile color changes and trends by analyzing the characteristics of interior color hues and tones. Heimtextil in the interior field was selected for color trend analysis from 1996 to 2000. The conclusions of this study are as follows: First, In terms of hue, warm colors like Y, YR, and R were most prominent in all four years. Some new colors, however, emerged with higher frequency. The frequency of GY and B increased in 96/97, GY, BG and PB in 97/98 PB and B in 98/99, and B and PB in 99/2000. Especially between 96/2000, the frequency of PB and B has been increased, although the frequency of GY has been decreased. Over the four years, P, RP, and Neutral showed medium frequency, and G and BG showed low frequency. Gold appeared as an accent color in two years while silver appeared in one year. In summary, warm colors like Y, YR, and R were most prominent in interior color. Second, In terms of tone, p, b, v, and sf were most prominent over the four years. Although 에 and ltg appeared with high frequency n general, a wide variation was presented. The tones such as p, dp, and v appeared with high frequency in the three years of 96/97, 97/98 b and sf in 97/98 ; lt, dk and sf in 98/99. On the other hand, a new trend emerged in 99/2000. In this year, ltg, sf, s, d and dkg appeared with high frequency unlike the other three years. In other words, soft or light tones, such as pale, soft, light, grayish, and deep, and strong tones, such as vivid and bright, appeared with high frequency.

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Characterization and Outbreak Pattern of Isolated Influenzavirus in Busan, 2000-2002 Years. (최근 3년 부산지역에서 분리한 인플루엔자바이러스의 특성 및 발생 양상)

  • 정명주;조경순
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.329-333
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    • 2003
  • Influenzavirus were isolated from patients with acute respiratory infections in Busan during 2000-2002 and characterized their antigenic properties. In 2000, 39 viruses were isolated and they were identified as Influenzavirus. Among the isolated influenzavirus, 23 were A type influenzavirus and 16 were B type influenzavirus. As a result of antigenic characterization, the influenzavirus were determined to A/Sydney/05/97 (H3N2)-like, A/Beijing/262/95 (H1N1)-like, and B/Harbin/07/94-like virus. In 2001, 56 viruses were isolated and all of the viruses were identified as influenzavirus. They were A/Panama/2007/99 (H3N2)-like and A/Newcaledonia/20/99 (H1N1)-like virus In 2002, 114 Influenzaviruses were isolated. Among the isolated influenzavirus, 83 were A type influenzaviruses, and 31 were B type influenzaviruses. They were A/Panama/2007/99 (H3N2)-like, A/Newcaledonia/20/99 (H1N1)-like and B/Beijing/243/97, B/Honkong/22/2001, B/Sichuam/379/99-like virus when determined their antigenic properties. These results may be useful for the establishment of reliable epidemic data.

A Classification and Analysis of the Articles in -From issue 1 to issue 99- (<수학교육>에 게재된 논문의 분류와 분석 -통권 1호부터 통권 99호까지 -)

  • 이강섭
    • The Mathematical Education
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    • v.42 no.2
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    • pp.247-258
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    • 2003
  • In this study, total 486 articles in the journal of the Korea Society of Mathematical Education Series A are classified and analyzed by MSC2000 and ZDM classification. The results are as follows: 1) 2000 Mathematics Subject Classification : (number of articles) 1) 2000 Mathematics Subject Classification : (number of articles) 97- : (31), 97A : (7), 97B : (40), 97D : (229), 97U(105) 2) ZDM classification : (number of articles) A : (36), B (45), C : (57), D : (145), E : (19), F : (27), G : (37) H : (7), I : (19), K : (18), M (4), N : (12), U : (60) 3) Some remarks and suggestions are proposed.

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Analysis of Genetic Polymorphism by Bloodtyping in Jeju Horse (혈액형에 의한 제주말의 유전적 다형성 분석)

  • Cho Gil-Jae
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.972-978
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    • 2005
  • The present study was carried out to investigate the blood markers of Jeju horses. The redcell cypes (blood groups) and blood protein types (biochemical polymorphisms) were tested from 102 Jeju horses by serological and electrophoretc procedure, and their phenotypes and gene frequencies were estimated. The blood group and biochemical polymorphism phenotypes observed with high frequency were $A^{af}\;(27.45\%$), $C^{a}\;(99.02\%$), $K^{-}\;(97.06\%$), $U^{a}\;(62.75\%$), $P^{b}\;(36.27\%$), $Q^{c}\;(47.06\%$), $D^{cgm/dghm}\;(13.73\%$), $D^{adn/cgm}\;(9.80\%$), $D^{ad/cgm}$\;(8.82\%$), $D^{dghm/dghm}(7.84\%$), $D^{cgm/cgm}(7.84\%$), $AL^{B}\;(48.04\%$), $GC^{F}\;(99.02\%$), $AlB^{K}\;(97.06\%$), $ES^{FI}\;(36.27\%$), $TF^{F2}\;(25.49\%$), $HB^{B1}\;(45.10\%$), and $PGD^{F}\;(86.27\%$) in Jeju horses, respectively. Alleles observed with high gene frequency were $A^{af}$ (0.3726), $A^{C}$ (0.2647), $C^{-}$ (0.5050), $K^{-}$ (0.9853), $U^{-}$ (0.6863), $P^{b}$ (0.4657), $Q^{c}$ (0.5294), $D^{cgm}$ (0.3039), $HB^{B1}$(0.6863), $PGD^{F}$ (0.9265), $AL^{B}$ (0.6912), $ALB^{K}$ (0.9852), $GC^{F}$ (0.9950), $ES^{I}$ (0.5000) and $TF^{F2}$ (0.4950) in Jeju horses, and sfecific alleles, $D^{cgm(f)}$ (0.0196), $HB^{A}$ (0.0147), $HB^{A2}$ (0.0196), $ES^{G}$ (0.0441), $ES^{H}$ (0.0098), $TF^{E}$TF'(0.0246), $TF^{H2}$ (0.0049) and $PGD^{D}$ (0.0098) were detected in Jeju horses. These preliminary results present basic information for detecting the genetic markers in Jeju horse. and developing a system for parentage verification and individuals identification in jeju horses.

Retrospective Study for the Isolation of Influenza Virus and Prevalence Period in Busan from 2000 to 2002 (2000년부터 2002년까지 부산지역에서 유행한 인플루엔자의 원인균 동정 및 유행시기)

  • Oh, Chang Hwan;Son, Byeong Hee;Kim, Ki Dae;Lee, Jung A;Kim, Sung Won;Cho, Kyung Soon
    • Clinical and Experimental Pediatrics
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    • v.48 no.3
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    • pp.260-265
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    • 2005
  • Purpose : During epidemics, influenza induces a high mortality and morbidity, and when influenza is prevalent, it is revealed by increased pneumonia, hospitalization due to influenza-like illness, and mortality in community. We aimed at the isolation of influenza virus and prevalence period in Busan from 2000 to 2002. Methods : For 3 years from 2000 to 2002, we analyzed the patterns of influenza virus, the occurrence distribution of influenza by age and sex and the prevalence period after cultivating the examined materials from throat smears and snivel, collected from patients in St. Benedict Hospital Pediatrics Department, from 10 monitoring hospitals, and from 16 public health centers. Results : For three years, a total of 209 strains of influenza virus were isolated. In 2000, there were A/sydney/05/97(H3N2)-like, A/Beijing/262/95(H1N1)-like and B/Harbin/07/94-like. In 2001, there were A/Panama/2007/99(H3N2)-like and A/Newcaledonia/20/99(H1N1)-like. In 2002, there were A/Panama/2007/99(H3N2)-like, A/Newcaledonia/20/99(H1N1)-like, B/Beijing/243/97, B/Honkong/22/2001 and B/Sichuam/379/99. The occurrence distribution by sexes were 14 males and 25 females in 2000, 23 males and 33 females in 2001, 57 males and 57 females in 2002. As for the occurrence distribution by ages, 0-10 years made up 48.4 percent in 2000, 11-20 years 33.93 percent in 2001, and below 10 years was 64.91 percent in 2002. As for the occurrence distribution by month, the rate was once high in January and somewhat high in April and by June, when there happened to be various viruses, though there was a low rate in 2000. On the other hand, the virus was concentrated in February and March in 2001. And in 2002, it happened high twice, in March and November. Conclusion : Influenza virus revealed frequent antigenic changes and infect children, especially those below 10 years of age from late fall to early spring. So we should consider appropriate prevention in children.

A molecular Genetic Variation among Intra-poplations of Korean shiner, Coreoleuciscus splendidus Mori (Cyprinidae) (한국산 쉬리, Coreoleuciscus splendidus (잉어과)의 종내 집단간 분자 유전 변이)

  • Song, Ho-Bok;Park, Gab-Man
    • Korean Journal of Ichthyology
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    • v.18 no.2
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    • pp.78-86
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    • 2006
  • We examined the genetic diversity in intra-populations of Korean shiner, Coreoleuciscus splendidus, in six major rivers (Bukhan, Namhan, Geum, Osipcheon, Nakdong and Seomjin river) of Korea based on two different mitochondrial genes, the mitochondrial cytochrome b and the 16S rRNA. Analysis of sequence variation in a 657-bp segment of the mitochondrial cytochrome b gene revealed deep divergence among populations (98.2~99.9%) and high genetic diversity from geographically isolated populations. Intra-specific variation in this 697-bp segment of the 16S rRNA gene sequences was very low and nearly identical. Six isolate populations of C. splendidus showed a high similarity (97.7%~99.7%). This result may be indicative of a complex history of connection and isolation of the rivers in the Korea peninsula.

Dose verification for Gated Volumetric Modulated Arc Therapy according to Respiratory period (호흡연동 용적변조 회전방사선치료에서 호흡주기에 따른 선량전달 정확성 검증)

  • Jeon, Soo Dong;Bae, Sun Myung;Yoon, In Ha;Kang, Tae Young;Baek, Geum Mun
    • The Journal of Korean Society for Radiation Therapy
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    • v.26 no.1
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    • pp.137-147
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    • 2014
  • Purpose : The purpose of this study is to verify the accuracy of dose delivery according to the patient's breathing cycle in Gated Volumetric Modulated Arc Therapy Materials and Methods : TrueBeam STxTM(Varian Medical System, Palo Alto, CA) was used in this experiment. The Computed tomography(CT) images that were acquired with RANDO Phantom(Alderson Research Laboratories Inc. Stamford. CT, USA), using Computerized treatment planning system(Eclipse 10.0, Varian, USA), were used to create VMAT plans using 10MV FFF with 1500 cGy/fx (case 1, 2, 3) and 220 cGy/fx(case 4, 5, 6) of doserate of 1200 MU/min. The regular respiratory period of 1.5, 2.5, 3.5 and 4.5 sec and the patients respiratory period of 2.2 and 3.5 sec were reproduced with the $QUASAR^{TM}$ Respiratory Motion Phantom(Modus Medical Devices Inc), and it was set up to deliver radiation at the phase mode between the ranges of 30 to 70%. The results were measured at respective respiratory conditions by a 2-Dimensional ion chamber array detector(I'mRT Matrixx, IBA Dosimetry, Germany) and a MultiCube Phantom(IBA Dosimetry, Germany), and the Gamma pass rate(3 mm, 3%) were compared by the IMRT analysis program(OmniPro I'mRT system software Version 1.7b, IBA Dosimetry, Germany) Results : The gamma pass rates of Case 1, 2, 3, 4, 5 and 6 were the results of 100.0, 97.6, 98.1, 96.3, 93.0, 94.8% at a regular respiratory period of 1.5 sec and 98.8, 99.5, 97.5, 99.5, 98.3, 99.6% at 2.5 sec, 99.6, 96.6, 97.5, 99.2, 97.8, 99.1% at 3.5 sec and 99.4, 96.3, 97.2, 99.0, 98.0, 99.3% at 4.5 sec, respectively. When a patient's respiration was reproduced, 97.7, 95.4, 96.2, 98.9, 96.2, 98.4% at average respiratory period of 2.2 sec, and 97.3, 97.5, 96.8, 100.0, 99.3, 99.8% at 3.5 sec, respectively. Conclusion : The experiment showed clinically reliable results of a Gamma pass rate of 95% or more when 2.5 sec or more of a regular breathing period and the patient's breathing were reproduced. While it showed the results of 93.0% and 94.8% at a regular breathing period of 1.5 sec of Case 5 and 6, it could be confirmed that the accurate dose delivery could be possible on the most respiratory conditions because based on the results of 100 patients's respiratory period analysis as no one sustained a respiration of 1.5 sec. But, pretreatment dose verification should be precede because we can't exclude the possibility of error occurrence due to extremely short respiratory period, also a training at the simulation and careful monitoring are necessary for a patient to maintain stable breathing. Consequently, more reliable and accurate treatments can be administered.