• Korean Journal of Veterinary Service
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• v.38 no.3
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• pp.167-171
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• 2015

Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.

• Journal of agriculture & life science
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• v.43 no.5
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• pp.39-42
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• 2009

Soybean is an important sources of plant proteins for human and animal nutrition. The use of soybean proteins has been expanded in the food industry due to their excellent nutritional benefits. But, Soybeans contain allergenic proteins that cause allergies to sensitive individuals. ${\beta}$-conglycinin(7S globulin) and glycinin(11S globulin) are the major components of storage protein in soybean. ${\beta}$-conglycinin consists of three subunits, ${\alpha}^{\prime}$, ${\alpha}$, ${\beta}$ and exhibits poorer nutritional and food processing properties than glycinin. There is a great deal of interest in the development of soybean lines with reduced amounts of ${\beta}$-conglycinin. The objective of this study was to determine the inheritance of ${\alpha}^{\prime}$-subunit protein in 7S globulin. F2 population was developed from the cross of "Jinpumkong2ho"(${\alpha}^{\prime}$-subunit presence) and PI506876(${\alpha}^{\prime}$-subunit absence) parent. Total 98 of F2 seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein by SDS-PAGE. Among 98 F2 seeds, 70 F2 seeds showed ${\alpha}^{\prime}$-subunit protein and 28 F2 seeds did not show ${\alpha}^{\prime}$-subunit protein. The segregation ratios of 3 : 1 for presence and absence of ${\alpha}^{\prime}$-subunit protein were observed(${\chi}^2=0.667$, P=0.414). These data indicate that presence and absence of ${\alpha}^{\prime}$-subunit protein is controlled by a single major gene and might be useful for strain selection of 7S protein reduced soybean.

• Analytical Science and Technology
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• v.10 no.5
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• pp.378-385
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• 1997

In order to clarify the functional role of Tyr7 in human glutathione S-transferase P1-1, we extensively investigated the effect of mutation of Tyr7 on the substrate specificity and inhibition characteristics. The mutational replacement of Tyr7 with phenylalanine lowered the specific activities with 1,2-dichloro-4-nitrobenzene and 1,2-epoxy-3-(p-nitrophenoxy) propane for GSH-conjugation reaction to 3~5% of the values for the wild-type enzyme. The pKa of the thiol group of GSH bound in Y7F was about 2.4 pK units higher than that in the wild-type enzyme. The $I_{50}$ of hematin for Y7F was similar to that for the wild-type enzyme and those of benastatin A and S-(2,4-dinitrophenyl)glutathione were only moderately decreased. These results suggest that Tyr7 is considered to be important the catalytic activities not only for GSH-chloronitrobenzene derivatives but also for GSH-epoxide conjugation reaction, rather than to binding of the substrates.

• The Journal of Sustainable Design and Educational Environment Research
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• v.17 no.1
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• pp.33-39
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• 2018

The number of elementary school classroom's students. It will be decreased to 21.1 OECD even and furthemore to 19.8 in 2030. Therfore fore the time being the number of elementary school classroom's students will be sustained in 20~22. But nowadays the classroom's area which is fitted the number of 30 is too big compare with the number of 20~22. This reserch is finding the profit module of elementary school's classroom of the number of 20~20. Using one student's unit and various displays of class by teaching methods, I found the conclusions as follows. 1st, the horizontal length of center line is 7,100~7,500 and the vertical length of center line is 7,000~8,000 in the classroom's area of a team of 2. 2nd, if you make adjustment those lenghts to 30cm module, horizontal length is transfered to 7.2m, 7.5m, and vertical length is transferred to 7.2m, 7.5m, 7.8m. Therefore unit classroom's module are $7.2m{\times}7.2m$, $7.5m{\times}7.5m$ in square, and $7.2m{\times}7.5m$, $7.2m{\times}7.8m$, $7.5m{\times}7.8m$ in rectangular. 3rd, the areas of modules are $7.2m{\times}7.2m(51.84m^2)$, $7.5m{\times}7.5m(56.25m^2)$, $7.2m{\times}7.5m(54m^2)$, $7.2m{\times}7.8m(56.16m^2)$, $7.5m{\times}7.8m(58.5m^2)$. Therfore th area of module is from $51.84m^2$ to $58.5m^2$ compared to nowadays' classrooms.

• Journal of Genetic Medicine
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• v.14 no.2
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• pp.62-66
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• 2017

Interchromosomal insertion of Y chromosome heterochromatin in an autosome was identified in a fetus and a family. A fetal karyotype was analyzed as 46,XX,dup(7)(?q22q21.1) in a referred amniocentesis at 16 weeks of gestation for advanced maternal age. In the familial karyotype analyses for identification of der(7), the mother, the first daughter and the maternal grandmother showed the same der(7) as the fetus's. CBG-banding was positive at 7q22 region of der(7) that indicated inserted material was originated from heterochromatin. The origin of heterochromatic insertion region in der(7) of the fetus and the mother was found in Yq12 region by fluorescent in situ hybridization with a DYZ1 probe. In the specific analysis of Y chromosomal heterochromatic region of ins(7;Y) of the mother, 15 sequence tagged sites from Yp11.3 region including SRY to Yq11.223 region was not detected. Final karyotypes of the mother, the first daughter and the maternal grandmother were reported as 46,XX,der(7)ins(7;Y)(q21.3;q12q12). All female carriers of ins(7;Y) in the family showed normal phenotype and the mother and the maternal grandmother were fertile. A healthy girl was born at term. We report a rare case of familial interchromosomal insertion of Y chromosome heterochromatin detected only in female family members with normal phenotype that was diagnosed prenatally.

• Journal of Periodontal and Implant Science
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• v.36 no.3
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• pp.627-637
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• 2006

이 연구는 rh BMP-7-immobilized substrates에 대한 백서 태자 두개관 세포의 반응을 석회화 결절 측정, 알카리 효소 분석, 역전사 중합반응 및 단백질 분석등으로 평가하여 다음과 같은 결과를 얻었다. 1. 배양 14일 째, 석회화 결절 형성율을 측정한 결과, rh BMP-7-immobilized substrates에서 대조군과 비교하여 더 많은 석회화 결절을 형성하였다. 2. 배양 7일에 염기성 인산 분해효소 활성도는 rh BMP-7-immobilized substrates에서 대조군에 비해 효소 활성도가 유의하게 높았다. 3. 역전사 중합반응의 결과에서 BSP 와 OCN 유전자 발현은 대조군보다 더 현저하였다. 4. 단백질 분석에서 rh BMP-7-immobilized substrates와 대조군 모두 Smad 1,5,8 단백질의 인산화를 활성화시키지 못했다. 이상의 결과 rh BMP-7-immobilized substrates는 백서 태자 두개관세포가 조골세포로의 분화와 석회화를 유도하며 따라서 rh BMP-7-immobilized substrates는 임프란트 주변의 골 형성에 유용하리라 사료된다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.1
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• pp.7-14
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• 1974

1. Stylochus ijimai spawns from May to October with peak spawning from July to September, P. obscurus spawns from June to October with peak spawning from July to September. 2. S. ijimai spawns approximately 96,000-132,000 eggs by one spawning. 1. Just after spawnings, the eggs of S. ijimai were $102\mu$ in diameter, and those of P. obscurus were $108\mu$. 4. S. ijimai and P. obscurus have simple eggs. S. ijimai develop indirectly having Gotte larvae, while P. obscurus develop directly. 5. These two species show very similar patterns of early developments from maturation division of eggs to the gastrula stage. 6. The zygots of S. ijimai reaches mesentoblast stage 48 hours after fertilization, and Gotte larvae hatch out 7 days after fertilization. The zygots of P. obscurus reaches mesentoblast stage 72 hours after fertilization, and juveniles hatch out 14 days after fertilization. 7. S. ijimai, have 14 days of planktonic larvae stages bearing strong phototacic behavior : P. obscurus have 7 days of planktonic life without phototacic behavior. 8. Newly hatched larvae of S. ijimai and P. obscurus are $138\mu\;and\;170\mu$ in length respectively. The early creeping forms of S. ijimai and P. obscurus are $152\mu\;and\;185\mu$ in length respectively. 9. In the early creeping stage S. ijimai are characterized by testing processes and flattening of the body. In the same stage P. obscurus lost 2 eye-spots in the cerebral area. 10. The early creeping larvae of these two species were found only in mud-flat substrates.

• Applied Biological Chemistry
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• v.34 no.3
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• pp.231-234
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• 1991

In the present investigation we have studied the effect of bear's gall on mammalian cells and demonstrated that COS-7 cells, which were derived Monkey kidney cells, had shown almost same extent of growth with 78 hrs in 10% FCS Dulbecco's Modified Eagle's medium with bear's gall and without bear's gall. But the hybridoma cells which were fused murine myeloma cells and the rat spleen cells for monoclonal antibody production died almost within 48 hrs. To investigate the effect of biosynthetic mechanism, cDNA were transfected to COS-7 cells, and it was shown that cDNA-transfected COS-7 cell had produced 30-40% less the amount of recombinant protein than the medium without bear's gall.

• Journal of Microbiology and Biotechnology
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• v.33 no.8
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• pp.998-1005
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• 2023

Streptococcus salivarius is a beneficial bacterium in oral cavity, and some strains of this bacterium are known to be probiotics. The purpose of this study was to investigate the anti-inflammatory effect and mechanism of S. salivarius G7 lipoteichoic acid (LTA) on lipopolysaccharide (LPS) and LTA of periodontopathogens. The surface molecules of S. salivarius G7 was extracted, and single- or co-treated on human monocytic cells with LPS and LTA of periodontopathogens. The induction of cytokine expression was evaluated by real-time PCR and ELISA. After labeling fluorescence on LPS and LTA of periodontopathogens, it was co-treated with S. salivarius LTA to the cell. The bound LPS and LTA were measured by a flow cytometer. Also, the biding assay of the LPS and LTA to CD14 and LPS binding protein (LBP) was performed. The surface molecules of S. salivarius G7 did not induce the expression of inflammatory cytokines, and S. salivarius G7 LTA inhibited the inflammatory cytokines induced by LPS and LTA of periodontopathogens. S. salivarius G7 LTA inhibited the binding of its LPS and LTA to cells. Also, S. salivarius G7 LTA blocked the binding of its LPS and LTA to CD14 and LBP. S. salivarius G7 has an inhibitory effect on inflammation induced by LPS or LTA of periodontopathogens, and may be a candidate probiotics for prevention of periodontitis.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.120-120
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• 2021

2020년 8월 7일부터 8월 8일까지 호우는 용담댐, 섬진강댐, 합천댐 하류 유역의 막대한 침수피해를 일으켰다. 이들 다목적 댐 유입량의 신뢰도 높은 모의는 홍수기 댐 운영 및 하류하천의 홍수 해석에 필수다. 여기서는 일 유출 모의 기반으로 개발된 ONE 모형을 10분 단위, 1시간 단위로 적용한 결과를 제시하고자 한다. 보통 홍수모의는 사상별로 실시하지만, 여기서는 1월1일부터 12월 31일까지 연속으로 모의한 결과에서 해당 홍수사상 결과를 제시하였다. 3개 다목적 댐의 홍수사상은 8월6일부터 8월 10일까지 5일간으로 설정하였다. 유역면적은 용담댐, 섬진강댐, 합천댐, 각각 930km², 763km², 925km², 총강우량은 각각 490.7mm, 451.9mm, 452.4mm, 첨두유입량은 10분 단위는 각각 4,872.7m³/s, 3,533.7.0m³/s, 2,776.0m³/s, 1시간 단위는 각각 4,394.9m³/s, 3,401.8m³/s, 2,745.6m³/s, 총유입량은 각각 3억8,836만m³, 3억1,324만m³, 3억2,816만m³였다. 첨두유입량 상대오차가 0일 때의 매개변수로 모의한 결과를 제시하며, 총유입량 상대오차(Vq), R², RMSE, NSE 등으로 평가하였다. 용담댐 결과는 10분 단위 경우 최대면적강우량 7.3mm, 첨두유입량 4,872.4m³/s, 총유입량 3억 8,138만m³, Vq 1.9%, R² 0.968, RMSE 207.347, NSE 0.978였고, 1시간의 경우 최대면적강우량 29.6mm, 첨두유입량 4394.9m³/s, 총유입량 4억157만m³, Vq -8.4%, R² 0.970, RMSE 186.962, NSE 0.982였다. 섬진강댐 결과는 10분 단위 경우 최대면적강우량 9.2mm, 첨두유입량 3,533.3m³/s, 총유입량 2억7,223만m³, Vq 18.4%, R² 0.885, RMSE 808.296, NSE 0.925였고, 1시간의 경우 최대 면적강우량 37.9mm, 첨두유입량 3401.6m³/s, 총유입량 2억7,029만m³, Vq 13.7%, R² 0.907, RMSE 285.544, NSE 0.936였다. 합천댐 결과는 10분 단위 경우 최대면적강우량 5.5mm, 첨두유입량 2,776.2m³/s, 총유입량 3억3,667만m³, Vq -2.7%, R² 0.941, RMSE 191.896, NSE 0.965였고, 1시간의 경우 최대면적강우량 17.0mm, 첨두유입량 2,746.7m³/s, 총유입량 3억1,333만m³, Vq 4.5%, R² 0.965, RMSE 140.739, NSE 0.981였다. 이상 ONE 모형으로 10분, 1시간 단위의 댐 홍수 유입량 모의결과는 높은 신뢰도를 나타냈다.