Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Substrate-immobilized bone morphogenic protein-7 peptides on titanium surface support the expression of extracellular matrix proteins

  • Kim, Young-Joon (Department of Periodontology, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Chan-Gil (Department of Periodontology, School of Dentistry, Chonnam National University) ;
  • Cui, De-Zhe (Dental Science Research Institute, Chonnam National University)
  • 김영준 (전남대학교 치의학전문대학원 치주과학 교실, 치의학 연구소) ;
  • 정찬길 (전남대학교 치의학전문대학원 치주과학 교실) ;
  • 최득철 (전남대학교 치의학 연구소)
  • Published : 2006.09.30
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Abstract

이 연구는 rh BMP-7-immobilized substrates에 대한 백서 태자 두개관 세포의 반응을 석회화 결절 측정, 알카리 효소 분석, 역전사 중합반응 및 단백질 분석등으로 평가하여 다음과 같은 결과를 얻었다. 1. 배양 14일 째, 석회화 결절 형성율을 측정한 결과, rh BMP-7-immobilized substrates에서 대조군과 비교하여 더 많은 석회화 결절을 형성하였다. 2. 배양 7일에 염기성 인산 분해효소 활성도는 rh BMP-7-immobilized substrates에서 대조군에 비해 효소 활성도가 유의하게 높았다. 3. 역전사 중합반응의 결과에서 BSP 와 OCN 유전자 발현은 대조군보다 더 현저하였다. 4. 단백질 분석에서 rh BMP-7-immobilized substrates와 대조군 모두 Smad 1,5,8 단백질의 인산화를 활성화시키지 못했다. 이상의 결과 rh BMP-7-immobilized substrates는 백서 태자 두개관세포가 조골세포로의 분화와 석회화를 유도하며 따라서 rh BMP-7-immobilized substrates는 임프란트 주변의 골 형성에 유용하리라 사료된다.

Keywords

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