• Title/Summary/Keyword: 7S

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Cytotoxic and Antitumor Constituents from Scutellaria indica

  • Bae, Ki-Hwan;Min, Byung-Sun;Ahn, Byung-Zun
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.245-245
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    • 1994
  • From the active fraction, cytotoxic constituents 1-5 were isolated and identified to be 2(S)-5,7-dihydroxy-8,2'-dimethoxyflavanone (1), wogonin (5,7-dihydroxy-8-methoxyflavone, 2), 5,7-dihydruxy-8,2'-dimethoxyflavone (3), 2(5) -5,7,2'-trihydroxy-8-methoxyflavanone (4), 2(S) - 5,2',5'-trihydroxy-7,8-dimethoxyflavanone (5), respectively, with those of specimens$\^$1-2)/

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Karyotype Analysis and Physical Mapping of rDNAs in Diploid Nicotiana plumbaginifolia (2배체 담배 Nicotiana plumbaginifolia의 핵형 분석과 rDNAs의 Physical Mapping)

  • Cho, Hye-Gyoung;Koo, Dal-Hoe;Kim, Soo-Young;Bang, Jae-Wook
    • Journal of Plant Biotechnology
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    • v.30 no.1
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    • pp.7-11
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    • 2003
  • Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using FISH (fluorescence in situ hybridization) technique were carried out in Nicotiana plumbaginifolia. Somatic chromosome number of N. plumbaginifolia was 2n=20 and kyarotype was composed of three pairs of metacentric chromosomes (1,2 and 7) and seven pairs of submetacentric chromosomes (3, 4, 5, 6, 8, 9 and 10). Length of chromosomes was ranged from 2.29 to 4.50 ${\mu}{\textrm}{m}$. Chromosome 1 and 2 were identified as satellite chromosomes. In FISH experiment, one pair of 5S rDNA signals was detected on the centromeric region of chromosome 2 and one pair of 45S rDNA signals was detected on the terminal region of chromosome 1.

Growth Inhibitory Effects of Chloride Salts and Organic Acid Salts Against Food-Borne Microorganisms (Chloride염 및 유기산 칼슘염의 식중독 미생물에 대한 증식 억제 효과)

  • 이나영;김용석;신동화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1233-1238
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    • 2003
  • The growth inhibitory effects of chloride salts and organic acid salts against six food-borne microorganisms (Bacillus cereus ATCC 11778, Escherichia coli O157:H7 ATCC 43894, Listeria monocytogenes ATCC 19111, Salmonella Typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, Vibrio parahaemolyticus ATCC 17802) were determined using Bioscreen C in broth medium. The growth inhibitory concentrations of sodium chloride and potassium chloride on B. cereus were 7 and 9%, respectively. E. coli O157:H7 and S. aureus were inhibited by treatment of 3% calcium chloride. Magnesium chloride showed growth inhibitory effect on B. cereus, S. Typhimurium, and S. aureus at 5%. The order of growth inhibition effects by organic acid salts was calcium propionate>calcium acetate>calcium lactate. Calcium chloride (3%) with 0.01% lactic acid showed strong inhibition on the growth of S. Typhimurium and exhibited stronger growth inhibition than calcium chloride alone (5%). We concluded that calcium chloride and calcium propionate had strong growth inhibitory activities and that calcium chloride and sodium chloride in combination with lactic acid had stronger inhibitory activities than that of chloride salts alone.

Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer

  • Kim, Yu-Jin;Park, Soo-Chul
    • BMB Reports
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    • v.44 no.2
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    • pp.113-117
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    • 2011
  • Previous studies suggest that EphA7 plays a critical role in neural tube closure or cortical progenitor apoptosis. In this report, enhancer trap assay was used to modify various EphA7 BAC clones and screen a large genomic region spanning 570 kb downstream of the EphA7 gene. We found that the dorsal midline-specific EphA7 enhancer resides on the 457D20 EphA7 BAC clone and is localized to a 35 kb genomic region in between +345.7 kb to +379.8 kb downstream of the EphA7 transcription start site. Identification of the EphA7 BAC clone containing a long-range dorsal midline enhancer may constitute a useful tool for investigating the biological functions of EphA7 in vivo.

Fabrication and Oxygen Permeation Properties of ${La_{1-x}Sr_{x}B_{1-{\gamma}}Fe_{\gamma}O_{3-{\delta}}$(B=Co, Ga) Perovskite-Type Ceramic Membranes (${La_{1-x}Sr_{x}B_{1-{\gamma}}Fe_{\gamma}O_{3-{\delta}}$(B=Co, Ga) 페롭스카이트 세라믹 분리막의 제조 및 산소투과특성)

  • 임경태;조통래;이기성;한인섭;서두원
    • Membrane Journal
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    • v.11 no.4
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    • pp.143-151
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    • 2001
  • We have fabricated mixed-ionic conducting membranes, L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ and L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ by the solid state method. Ceramic membranes consisted of perovskite-type structures and exhibited high relative density, >95%. Especially, dense L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ layer was coated on the L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes by using screen printing technique in order to improve oxygen ion flux. We measured oxygen ion flux on uncoated L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/, uncoated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/, and coated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes. The L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ membranes showed the highest flux, 0.26 mL/min.$\textrm{cm}^2$ at 90$0^{\circ}C$, after steady state had been reached. The oxygen flux of coated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes showed higher value, 0.19 mL/min.$\textrm{cm}^2$ at 95$0^{\circ}C$. This flux was as much as 2 or 3 times higher than those of uncoated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes. 3-$\delta$/ membranes.X> 3-$\delta$/ membranes.membranes.

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Selection of a Soybean Line with Brown Seed Coat, Green Cotyledon, and Tetra-Null Genotype (갈색종피와 녹색자엽 및 Tetra Null 유전자형을 가진 콩 계통 선발)

  • Sarath Ly;Hyeon Su Oh;Se Yeong Kim;Jeong Hwan Lee;Jong Il Chung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.114-120
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    • 2023
  • Soybean is the one of the most important crops for providing quality vegetable protein to umans and livestock. Soybean cultivars with a brown seed coat have a wide range of antioxidant benefits because of the flavonoid components. However, they also contain lectin, 7S α′ subunit, lipoxygenase, and Kunitz trypsin inhibitor (KTI) proteins that can be allergenic and digestive inhibitors and reduce processing aptitude. Genetic removal of these four proteins is necessary in soybean breeding. Therefore, this study was conducted to select a new line with brown seed coat, green cotyledon, and tetra-null genotype (lecgy1lox1lox2lox3ti) for lectin, 7S α′ subunit, lipoxygenase, and KTI proteins in the mature seed. Five germplasms were used to create breeding population. From a total of 58 F2 plants, F2 plants with lele genotype were selected using a DNA marker, and F3 seeds with a brown seed coat, green cotyledon, and the absence of 7S α′ subunit protein were selected. Three lines (S1, S2, and S3) were developed. Genetic absence of lectin, 7S α′ subunit, lipoxygenase, and KTI proteins was confirmed in F6 seeds of the three lines, which had a brown seed coat, green cotyledons, and a white hilum. The 100 seed weights of the three lines were 26.4-30.9 g, which were lower than 36 g of the check cultivar - 'Chungja#3'. The new S2 line with 30.9 g hundred seed weight can be used as a parent to improve colored soybean cultivars without antinutritional factors such as lectin, 7S α′ subunit, lipoxygenase, and KTI proteins.

Studies on the Antibacterial Activity of Poria cocos (복령의 항균력(抗菌力)에 관한 연구(硏究))

  • Lee, Kook-Sung;Lee, Min-Woong;Lee, Ji-Yul
    • The Korean Journal of Mycology
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    • v.10 no.1
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    • pp.27-31
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    • 1982
  • The antibacterial activity of Poria cocos against the human pathogenic bacteria and general bacteria was observed. The results obtained were as follows; 1. There wasn't any anti­bacterial activity at the Poria cocos powders, pieces and its extracts in every different kinds and places of origin. 2. Hyphae of Poria cocos in every different kinds and places of origin had the antibacterial activity against four Gram positive bacteria, i.e., Staphylococcus aureus, S. epidermidis, Bacillus subtilis and Streptococcus faecalis, on the other hand, hadn't the antibacterial activity against six Gram negative bacteria, Salmonella typhi, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaria and Klebsiella pneumoniae. 3. The average inhibitory zone was 13.6mm for S. aureus, 14.7mm for S. epidermidis, 12.7mm for B. subtilis, and 13.2mm for S. faecalis at the concentration of $335{\mu}g$ of hyphae of Poria cocos.

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Evaluation of the Neural Fiber Tractography Associated with Aging in the Normal Corpus Callosum Using the Diffusion Tensor Imaging (DTI) (확산텐서영상(Diffusion Tensor Imaging)을 이용한 정상 뇌량에서의 연령대별 신경섬유로의 변화)

  • Im, In-Chul;Goo, Eun-Hoe;Lee, Jae-Seung
    • Journal of the Korean Society of Radiology
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    • v.5 no.4
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    • pp.189-194
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    • 2011
  • This study used magnetic resonance diffusion tensor imaging (DTI) to quantitatively analyze the neural fiber tractography according to the age of normal corpus callosum and to evaluate of usefulness. The research was intended for the applicants of 60 persons that was in a good state of health with not brain or other disease. The test parameters were TR: 6650 ms, TE: 66 ms, FA: $90^{\circ}$, NEX: 2, thickness: 2 mm, no gap, FOV: 220 mm, b-value: $800s/mm^2$, sense factor: 2, acquisition matrix size: $2{\times}2{\times}2mm^3$, and the test time was 3 minutes 46 seconds. The evaluation method was constructed the color-cored FA map include to the skull vertex from the skull base in scan range. We set up the five ROI of corpus callosum of genu, anterior-mid body, posterior-mid body, isthmus, and splenium, tracking, respectively, and to quantitatively measured the length of neural fiber. As a result, the length of neural fiber, for the corpus callosum of genu was 20's: $61.8{\pm}6.8$, 30's: $63.9{\pm}3.8$, 40's: $65.5{\pm}6.4$, 50's: $57.8{\pm}6.0$, 60's: $58.9{\pm}4.5$, more than 70's: $54.1{\pm}8.1mm$, for the anterior-mid body was 20's: $54.8{\pm}8.8$, 30's: $58.5{\pm}7.9$, 40's: $54.8{\pm}7.8$, 50's: $56.1{\pm}10.2$, 60's: $48.5{\pm}6.2$, more than 70's: $48.6{\pm}8.3mm$, for the posterior-mid body was 20's: $72.7{\pm}9.1$, 30's: $61.6{\pm}9.1$, 40's: $60.9{\pm}10.5$, 50's: $61.4{\pm}11.7$, 60's: $54.9{\pm}10.0$, more than 70's: $53.1{\pm}10.5mm$, for the isthmus was 20's: $71.5{\pm}17.4$, 30's: $74.1{\pm}14.9$, 40's: $73.6{\pm}14.2$, 50's: $66.3{\pm}12.9$, 60's: $56.5{\pm}11.2$, more than 70's: $56.8{\pm}11.3mm$, and for the splenium was 20's: $82.6{\pm}6.8$, 30's: $86.9{\pm}6.4$, 40's: $83.1{\pm}7.1$, 50's: $81.5{\pm}7.4$, 60's: $78.6{\pm}6.0$, more than 70's: $80.55{\pm}8.6mm$. The length of neural fiber for normal corpus callosum were statistically significant in the genu(P=0.001), posterior-mid body(P=0.009), and istumus(P=0.012) of corpus callosum. In order of age, the length of neural fiber increased from 30s to 40s, as one grows older tended to decrease. For this reason, the nerve cells of brain could be confirmed through the neural fiber tractography to progress actively in middle age.

Syntheses and Optical Properties of the Water-Dispersible ZnS:Mn Nanocrystals Surface Capped by L-Aminoacid Ligands: Arginine, Cysteine, Histidine, and Methionine

  • Lee, Ju-Ho;Kim, Yong-Ah;Kim, Ki-Moon;Huh, Young-Duk;Hyun, June-Won;Kim, H.S.;Noh, S.J.;Hwang, Cheong-Soo
    • Bulletin of the Korean Chemical Society
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    • v.28 no.7
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    • pp.1091-1096
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    • 2007
  • Water dispersible ZnS:Mn nanocrystals were synthesized by capping the surface of the nanocrystals with four kinds of aminoacids ligands: arginine, cystein, histidine, and methionine. The aminoacids capped ZnS:Mn nanocrystal powders were characterized by XRD, HR-TEM, EDXS, and FT-IR spectroscopy. The optical properties of the aminoacids capped ZnS:Mn colloidal nanocrystals were also measured by UV/Vis and solution photoluminescence (PL) spectroscopies in aqueous solvents. The solution PL spectra showed broad emission peaks around 575 nm (orange light emissions) with PL efficiencies in the range of 4.4 to 7.1%. The measured particle sizes for the aminoacid capped ZnS:Mn nanocrystals by HR-TEM images were in the range of 5.3 to 11.7 nm.

Lessons from 7th Fleet's Naval Ship Collisions to the Republic of Korea Navy (미 7함대 해군함정 사고 경과와 교훈)

  • Moon, Changhwan
    • Strategy21
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    • s.43
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    • pp.201-224
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    • 2018
  • The purpose of this study is to analyze the causes of a series of naval ship collisions in the United States 7 Fleet and diagnose the problems of the Republic of Korea Navy(ROKN). The ROKN should thoroughly analyze the lessons of the U.S. Navy's collisions and try to avoid the same cases in the future. In chapter 2, this article briefly explains how the USS Fitzgerald(DDG62) and USS John S. McCain(DDG56) have a collision with the civilian vessels. This paper uses three official documents published by the U.S. Navy in 2017 to get details of incidents. In chapter 3, this paper analyzes the cause of the two incidents. According to the official reports, two aegis ships have complex problems. First, executives significantly lacked the seamanship and qualification. Second, there were no organizational culture and teamwork to form the right decisions. Also, there was a structural problem inside the 7 Fleet itself. In chapter 4 and 5, this study applies some lessons to the ROKN. Especially, this chapter suggests policy recommendations to prevent the similar incidents from occurring in the ROKN. The ROKN should be aware of the possibility that the sorts of incident could occur any time.