• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1508-1518
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• 2021

Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

• 생명과학회지
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• 제19권6호
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• pp.765-769
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• 2009

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기 서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기 서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), MIL, Q3R, P5S, Q14H, D25N, 127R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집 단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다.

• Current Research on Agriculture and Life Sciences
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• 제4권
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• pp.36-41
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• 1986

대장균(大腸菌)과 효모(酵母)의 셔틀 벡터인 YIp, YRp, YEp를 S. cerevisiae MC 16, DBY747에 형질전환(形質轉換)시켰다. 이들 벡터들을 대장균(大腸菌)에 형질전환(形質轉換)시켰을 때 그 빈도(頻度)가 YIp5, YIp26, YEp13, YRp7에서 각각 $5.1{\times}10^{-4}$, $1.5{\times}10^{-3}$, $3{\times}10^{-3}$, $1.3{\times}10^{-3}$으로 나타났다. YEp13을 MC16과 DBY747에 Ito 법(法)으로 형질전환(形質轉換)시켰을 때 MC16, DBY747에서의 빈도(頻度) 각각 $3.3{\times}10^{-4}$, $1.2{\times}10^{-4}$으로 나타났다. DBY747을 수용세포(受容細胞)로 하여 YRp7과 YIp26을 환상(環狀)으로 각각 형질전환(形質轉換)시켰을 때 그 빈도(頻度)는 각각 $3{\times}10^{-6}$, $6{\times}10^{-8}$이하로 나타났다. DBY747을 수용세포(受容細胞)로 하여 YEp13과 YIp26을 선상(線狀)으로 절단하여 $Li^+$ 처리(處理)한 S. cerevisiae에 cotransformation을 하였을 때 YIp26+YEp13 ($Leu^+$, $Ura^+$)의 cotransformant는 $1{\times}10^{-5}$ 빈도(頻度)가 나왔으며 YIp26과 YEp13에 대한 각각의 빈도(頻度)는 $10^{-7}$ 이하, $5{\times}10^{-5}$ 빈도(頻度)로 나타났다. 또 YIp5와 YEp13, YIp26과 YRp7을 원형질체화(原形質體化)한 S. cerevisiae DBY747에 cotransformation 하였을 때 빈도(頻度)는 YIp5+YEp13은 $4{\times}10^{-6}$으로 나오고 YIp26+YRp7에서 $1.5{\times}10^{-6}$으로 나타났다.

• Tribology and Lubricants
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• 제14권3호
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• pp.17-23
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• 1998

Friction materials with three different formulations containing different solid lubricants were investigated to study the role of lubricants on the friction performance. The three friction materials contained graphite 10 vol.%, graphite 7 vol.%+$MoS_2$ 3 vol.%, and graphite 7 vol.%+$Sb_2S_3$ 3 vol.%, respectively, with the same amount of other ingredients. Results of this work showed that each formulation with different lubricants had unique advantages and disadvantages. The friction materials containing graphite 7 vol.%+$MoS_2$ 3 vol.% and graphite 7 vol.%+$Sb_2S_3$ 3 vol.% showed better resistance to fading and improved friction stability compared to the friction materials containing graphite only as a lubricant. However, the friction materials with two lubricants (graphite+$MoS_2$ or $Sb_2S_3$) showed disadvantages on DTV generation and rotor wear.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1998년도 제27회 춘계학술대회
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• pp.328-334
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• 1998

In this work, friction materials with three different formulations containing different amounts of the solid lubricants were investigated to study the role of lubricants on the friction performance. The three friction materials contained graphite 10 vol. %, graphite 7 vol. % + MoS$_2$ 3 vol.%, and graphite 7 vol. % + $Sb_2S_3$ 3 vol. %, respectively, with the same amount of other ingredients. Results of this work showed that each formulation with different lubricants had unique advantages and disadvantages. The friction materials containing graphite 7 vol. % + MoS$_2$ 3 vol. % and graphite 7 % + $Sb_2S_3$ 3 vol. % showed better resistance to fading and improved friction stability compare to the friction materials containing graphite only as a lubricant. However, the friction materials with two lubricants (graphite + MoS$_2$ or $Sb_2S_3$) showed disadvantages on stick-slip phenomena, amplitude of torque, and rotor wear.

• 한국환경교육학회지:환경교육
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• 제13권2호
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• pp.103-113
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• 2000

The 7th national curriculum for environmental education in elementary and secondary school focuses on consideration of learner's ability, learner's activities, and learner's region of everyday life. The contents of environmental education are scattered subjects in elementary schools and an independent subject as 'Environment'in middle school and 'Environment and Ecology'in high school of the second school. With upgrading of schools, the aims and activities of environmental learning move up from value-centered, through activity-oriented and to cognition-centered.

• International journal of advanced smart convergence
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• 제7권2호
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• pp.127-134
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• 2018

This study found the digital divide of Korean elderly persons on the basis of the 2013-2017 survey data of the perception of media information adopters. The studied media were traditional ones, such as newspaper and TV. The media dependence of those in their 20s to 60s was compared and analyzed. As a result, regarding their dependence on newspaper, elderly persons in their 60s had 20.7% on average, 13.6% higher than the percentage (7.1%) of young persons. Regarding their dependence on TV, those in their 60s had 97.6% and those in their 20s had 63.7%, so that there was a wide gap of 33.9% on average. The smartphone penetration rate of those in their 60s was 61.3%(2015-2017) on average, which tended to rise. The elderly's dependence on traditional media featured Digital Divide which is found in the informatization process. Accordingly, in order to solve their digital divide, it is required to expand education in line with their knowledge level through remote online centers, develop inclusive ICT for convenience, and change philosophical policy paradigm in the dimension of quality of life.

• 한국식품조리과학회지
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• 제10권3호
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• pp.260-266
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• 1994

The object of this study was investigation of the appreciable cooking condition in seven wild grasses, an evening primerose(Oenothera lamarckiana Seringe, S$_1$), a spiderwort(Commelina Communis L. S$_2$), the flower of a convolvulus(Calystegia Ja-ponica form Vulgaris gaea, S$_3$), So Ru Jaeng Yi(Portulaca Oleracea, S$_4$), Shoe Bi Rum(Portulacae Oleracea L, S$_5$), O Yi Pul(Sanguisorb aOfficinalis, S$_6$), Jip Sin Na Mul(Agrimonia poisa var. Japonica Nakai, S$_7$), in order to find out the importance as an useful vegetables. In this work 7 wild grasses with different soaking solutions and boiling conditions were examined to investigation the preference factors by sensory evaluation. Results of sensory evaluation for samples showed a significant difference at 1% and 5% level There were high significant differents in samples boiled for different time about astringent taste, bitter taste, perfume, green color and grassy smell at 1% and 5% level. In cooking methods of 7 samples, Namul was liked best and Guk, Salad and Fritter were followed. The condiments which were used for seasoning Namul were Gochujang. Soy Paste, Salt. Gochujang was most preferred.

• 한국응용곤충학회지
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• 제35권1호
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• pp.80-84
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• 1996

곤충병원성선충과 곰팡이를 이용한 파리의 생물적 방제 가능성을 검토한결과, 여과지상에서의 큰집파리에 대한 고충병원성선충의 병원력은 동래에서 분리한 Steinernema glaseri strain이 $96.7\pm$2.8%의 치사유을 보여 가장 효과가 좋았으며, 다음이 S. carpocapsae로서 $90.0\pm$0.0%였고 함양에서 분리한 Heterorhabditis bacteriophora strain은 $86.7\pm$2.7%, 포천에서 분리한 S. carpocapsae strain은 $70.0\pm$9.4%였다. 농가 화장실에 곤충병원성선충 260,000마리씩을 처리한 경우, H. bacteriophora 함양 strain이 100%, S. glaseri 동래 strain이 $76.9\pm$3.9%, S. carpocapsae 포천 strain이 $58.5\pm$6.1%의 파리유충을 치사시켰다. 곤충병원성곰팡이 Beauveria brongniartii 7.0$\times${TEX}$10^{9}${/TEX} cfu를 단독처리한 농가 화장실에서는 집파리유충의 치사율이 $73.6\pm$0.1%였으나, S. carpocapsae포천 strain이 130,000마리와 곰팡이 혼합처리에서는 77.8$\pm$3.9%, H. bacteriophora 함양 strain 130,000마리와 곰팡이 혼합처리에서는 $77.7\pm$5.1%의 치사율을 보여, 파리방제의 곤충병원성선충과 곰팡이의 이용가능성이 있었다.

• KSBB Journal
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• 제17권6호
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• pp.537-542
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• 2002

50가지 생약재를 95% 에탄올과 물추출하여 식중독 세균인 S. aureus와 E. coli O157:H7에 대한 항균활성을 조사하였다. 한천배지확산법에 따른 생육저해효과를 측한 결과, S. aureus 에 대하여 우슬>목단피>지실>황백, 건강, 민들레 순으로, E. coli O157:H7에 대하여 목단피＞우슬>건강>지실, 황백 순으로 활성을 나타내었다. MIC는 S. aureus에 대하여 우슬이 156.25 $\mu$g/mL, 목단피와 황백이 625 $\mu$g/mL로 나타났으며, E. coli O157:H7에 대하여 우슬과 목단피의 MIC는 각각 625, 312.5 $\mu$g/mL로 높은 항균력을 나타내었다. 가장 높은 활성을 보인 우슬과 목단피를 용매분획(hexane, CHC1$_3$, ethyl acetate, butanol)하여 한천배지 확산법에 의한 두 균에 대한 생장저해 효과를 측정하였다. 우슬은 ethyl acetate 분획물에서, 목단피는 CHC1$_3$과 ethyl acetate 분획물에서 각각 높은 활성을 나타내었다.