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http://dx.doi.org/10.4014/jmb.2106.06031

Hsa_Circ_0001947/MiR-661/DOK7 Axis Restrains Non-Small Cell Lung Cancer Development  

Bao, Yuyan (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Yu, Yanjie (Sanmen Market Supervisory Authority)
Hong, Bing (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Lin, Zhenjian (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Qi, Guoli (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Zhou, Jie (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Liu, Kaiping (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Zhang, Xiaomin (Pharmaceutical Preparation Section, Sanmen People's Hospital)
Publication Information
Journal of Microbiology and Biotechnology / v.31, no.11, 2021 , pp. 1508-1518 More about this Journal
Abstract
Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.
Keywords
Non-small cell lung cancer; hsa_circ_0001947; miR-661; downstream of tyrosine kinase 7;
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