• Title/Summary/Keyword: 70% 알코올

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Glycoprotein in the Fruit Body of Sarcodon aspratus (능이자실체의 Glycoprotein)

  • Cho, Nam-Seok;Choi, Tae-Ho;Cho, Hee-Yeon;Leonowicz, Andrzej
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.5
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    • pp.51-58
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    • 2004
  • This study was performed to investigate compositions of inorganic elements, amino acids and glycoprotein fractions as biological substances in fruit body of Sarcodon aspratus. The fruit body of Sarcodon aspratus contained Ca, Mg, Zn, Mn, Fe, Cu, and Pb, in particular high Ca and Na. Hot water extracts consisted of 54% of polysaccharide fraction and 32.6% of protein. In amino acids composition, fourteen free amino acids were detected, mainly glutamic acid, alanine and arginine. Fifteen kinds of total amino acids were contained with major components of glutamic acid, aspartic acid, serine and threonine. Concerned to glycoprotein extraction, 95% ethyl alcohol concentration gave the highest yields with 70.6% sugar fraction, 332% glycoprotein. Different ethyl alcohol concentration resulted in different protein precipitations, and lower concentration ethyl alcohol in the range of 30 to 70% gave more than 92% of higher sugar fraction. Crude glycoprotein (GP) was fractionated by P fraction of more than MW 300,000, P-1 fraction unadsorbed by DEAE-Sephadex, P-2 fractionated from P-1 by Sepharose 2B gel chromatography and P-3 fraction adsorbed by DEAE-Sephadex. Total sugars were increased and protein contents decreased during fractionation. GP and P-3 contained glucose, galactose, mannose and fucose. GP had high glucose with high contents of glutamic acid, serine, alanine and glycine. P-3 fraction contained high mannose with aspartic acid, glutamic acid, and glycine. P-2 fraction was 700,000 MW with high glucose and fucose, and low protein of 1.1%, high amounts of aspartic acid, glutamic acid and alanine, but no mannose and no cysteine.

Quality Characteristics of Yakju as Affected by Rice Nuruk with Different Degrees of Milling (도정도별 쌀누룩 약주의 품질특성)

  • Choi, Jeong Sil;Yeo, Soo Hwan;Jeong, Seok Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.7
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    • pp.848-856
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    • 2017
  • This study aimed to investigate the effect of rice nuruk prepared from rice with different degrees of milling (0, 10, 30, 50, and 70%) on physicochemical qualities of yakju. The results indicated significant differences in the qualities of yakju prepared using rice nuruk from rice with different degrees of milling. Specially, pH, amino acidity, brownness, b value, and color difference were significantly reduced as the degrees of milling increased, whereas contents of alcohol and volatile acid, reducing sugar, and L value were increased. Malic acid, succinic acid, and lactic acid were the main organic acids in yakju, and there were significant differences among the treatments in malic acid, lactic acid. However, there was no tendency according to different degrees of milling. In addition, contents of total free amino acids and nitrogen compounds showed no tendency according to degrees of milling, whereas the content of aspartic acid showed a tendency to decrease significantly. The content of 2-phenyl alcohol in the volatile flavor components showed a tendency to increase significantly with higher degree of milling of rice nuruk, whereas contents of n-propanol and iso-amyl alcohol tended to decrease significantly.

Physiochemical Properties, and Antioxidative and Alcohol-metabolizing Enzyme Activities of Nectarine Vinegar (천도복숭아 식초의 이화학적 특성과 항산화 및 알코올 대사 효소 활성)

  • Jung, Kyung Im;Jung, Han Nah;Ha, Na Yeon;Choi, Young Ju
    • Journal of Life Science
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    • v.28 no.10
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    • pp.1193-1200
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    • 2018
  • This study investigated the physiochemical properties, antioxidative, nitrite-scavenging, and alcohol metabolism enzyme activities of nectarine vinegar prepared by a traditional fermentation method. The pH of nectarine vinegar was 3.70, the sugar content was $8.87^{\circ}Brix$, and the total acidity was 6.29%. Among organic acids detected, acetic acid was highest at 32.42 mg/ml, followed by lactic acid, malic acid, and succinic acid. Total phenol content of the nectarine vinegar was $121.84{\mu}g$ tannic acid equivalents (TAE)/100 ml. The antioxidative effects of muskmelon vinegar were measured using 1,1-Diphenyl2-picrylhydrazy (DPPH) radical-scavenging activity and superoxide dismutase (SOD) assay. DPPH of nectarine vinegar was increased in a dose-dependent manner, which was 84.47% at 40% concentration. SOD activity was increased in a dose-dependent manner, which was 89.06% at 60% concentration. Nitric scavenging activities of nectarine vinegar were 94.17%, 76.91%, and 20.21% at pH values 1.2, 3.0, and 6.0 at 100% concentration, respectively. The effects of nectarine vinegar on alcohol-metabolism were determined by measuring the generation of reduced nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). ADH and ALDH activities of nectarine vinegar were increased in a dose-dependent manner, which were 153.61% and 178.20 % at 60% concentration, respectively. These results suggest that nectarine vinegar has great potential as a resource for high quality functional health beverages.

Preparation and Characterization of Antimicrobial Films Using Water Soluble Polymer (수용성 고분자를 이용한 항균 필름의 제조 및 특성 연구)

  • Choi, Jun Ho;Choi, Yoo Sung;Oh, Il Hong;Kim, Maeng Su;Lee, In Hwa
    • Korean Chemical Engineering Research
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    • v.49 no.4
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    • pp.449-455
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    • 2011
  • This study was performed to develop antimicrobial films using polyvinyl alcohol and methyl cellulose. Methyl cellulose and polyvinyl alcohol films plasticized with PEG(polyethylene glycol) were prepared by solvent casting process under addition of 0.025~1.0 wt% ampicillin and 0.1~1.0 wt% streptomycin as an antimicrobial agent. The mechanical properties of prepared films were examined by universal testing machine(UTM). Tensile strength of methyl cellulose films was 15.44~21.70 $N/mm^2$. Tensile strength of PVA(15 wt%) film was 20.2~51.5 $N/mm^2$, and the tensile strength of the antimicrobial films were decreased linearly with increasing the antibiotic loading amount up to 1 wt%. Antimicrobial activities of PVA and methyl cellulose films containing ampicillin and streptomycin through the disc diffusion test for the Staphylococcus aureus and Escherichia coli. The antimicrobial activity of methyl cellulose films and PVA containing ampicillin were higher than that of containing streptomycin methyl cellulose films. The results indicate the films may be a proper materials for antimicrobial packing applications.

Development of Red Wine Using Domestic Grapes, Campbell Early. Part (I) - Chracteristics of Red Wine Fermentation Using Campbell Early and Different Sugars - (국산 포도(Campbell Early)를 이용한 적포도주의 개발(I) - 첨가되는 당을 달리한 Campbell Early 포도주의 발효특성 -)

  • Kim, Jae-Sik;Sim, Ji-Young;Yook, Cheol
    • Korean Journal of Food Science and Technology
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    • v.33 no.3
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    • pp.319-326
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    • 2001
  • Red wines were prepared with Campbell Early harvested at Youngdong, Chungbuk Province in 1999 of which average sugar content and total acidity(tartaric acid %) were $12^{\circ}Brix$ and 0.7%, respectively. In order to investigate the effect of addition of various sugars on the quality of red wine, sucrose, xylitol, glucose, corn syrup, high fructose corn syrup(HFCS) and isomaltooligosaccharide(IMO) were added to musts to have $21^{\circ}Brix$ of sugar content. Fermentation of red wine in which glucose was added was faster than any other sugars with the final ethanol content of 12%(v/v). Wines to which sucrose and HFCS were added showed similar fermentation rates to glucose added one but alcohol contents were 10.3%(v/v) and 11.2%(v/v), respectively. Alcohol contents of wines made with xylitol, corn syrup and IMO was relatively low to about 7% (v/v) after fermentation. The pH values of wines were almost unchanged in all treatments during fermentation and the total acidities of wines were decreased from 0.7% to lower than 0.3%. The colors of wines were changed to redder and darker during fermentation. In sensory evaluation xylitol added wine showed the best preference and kept xylitol unfermented in it.

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Fermentation Characteristics for Preparation of Distilled Liquor Made of Mixed Grains (혼합잡곡 증류주 제조를 위한 발효 특성)

  • Lee, Dae Hyoung;Jung, Jae Woon;Lee, Yong Sun;Seo, Jae Soon;Park, In Tae
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.446-455
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    • 2014
  • In this study, we aimed to develop mixed grain-derived hard liquor with enhanced favor and taste. First, fermentation characteristics analysis according to the grain used showed that rice and adlay produced high alcohol outputs of 15.2% and 13.3%, respectively, while that of barley was low at 5.6%. In an experiment in which different amounts sorghum flour were added, the sample without sorghum flour showed an alcohol content of 19.0%. The effect of adding up to 7% ipkuk (koji) was examined in a sensory test. Comprehensive preference increased with increasing amounts of added ipkuk. Fermentation characteristics analyses of distilled liquor into which milled rice (5% degrees of milling of unpolished waxy rice) was added showed that alcohol content and acidity were not greatly affected by its addition. Finally, an analysis of aromatic ingredients showed that the percentage of ethyl palmitate, which produces a smooth aroma, was the highest in distilled liquor made of mixed grains.

Chemical Compositions of Korean Ginseng with Special Reference to the Part of Ginseng Plant (고려인삼의 부위별 성분함량)

  • 장진규;이광승
    • Journal of Ginseng Research
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    • v.11 no.1
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    • pp.84-89
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    • 1987
  • The contents of some chemical constituents in several parts of Panax ginseng were investigated. Each part of ginseng was extracted with 70% ethanol and then water. The yield of extract was the highest in fine root, and relatively low in roughly dried ginseng and white ginseng, On the other hand, the contents of total sugars in white ginseng and seedling ginseng were high, but low in leaf and peel. The contents of crude protein in roughly dried ginseng and white ginseng were high, but those in leaf, rhizome (nod) and peel were low. The content of crude fat was higher in leaf than in other parts of ginseng plants and that was the lowest in fine root. Among free sugars, the content of fructose was high in leaf and rhizome, but that was the lowest in fine root. In the case of glucose content, leaf contained the highest amount, but fine root did the lowest. Sucrose contents in white, roughly dried and lateral roots were high, whereas that in leaf was low.

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Nucleotide Sequence of an Extracellular Phospholipase D Gene from Streptomyces somaliensis and Transphosphatidylation Activity of Its Enzyme (Streptomyces somaliensis가 생산하는 세포외 Phospholipase D의 유전자 서열 분석과 Transphosphatidylation 활성 특성)

  • Jeong Sujin;Lee Sun-Hee;Uhm Tai-Boong
    • Korean Journal of Microbiology
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    • v.40 no.3
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    • pp.211-216
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    • 2004
  • A bacterial strain JE-ll found to produce active extracellular phospholipase D (PLD) was selected from the soil isolates. It was identified as Streptomyces somaliensis on the basis of 16S rDNA sequence analysis, morphological and physiological characteristics. The gene (sspld) encoding S. somaliensis PLD was isolated and characterized. The open reading frame was suggested to encode 538 amino acids with a signal peptide of 33 amino acids. The deduced amino acid sequence of the sspld shared a sequence similarity of 70-88% with PLDs of other Streptomyces sp. so far reported. The PLD converted phosphatidylcholine to phosphatidylglycerol or phosphatidylserine with the yield of 96 to 99% (㏖/㏖), but did not act on inositol or ethanolamine as a transphosphatidylation donor.

The Development of Natural Pigment with Mulberry Fruit as a Food Additive (뽕나무 오디를 이용한 cyanidin-3-glucoside 함유 천연식용색소 개발)

  • Kim, Hyun-Bok;Kim, Sun-Lim;Koh, Seong-Hyouk;Seok, Young-Seek;Kim, Yong-Soon;Sung, Gyoo-Byung;Kang, Pil-Don
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.18-22
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    • 2011
  • Study on extraction and color characteristics of mulberry fruit pigment(C3G; cyanidin-3-glucoside) was performed to increase utilization as new source of natural food colorant. C3G was extracted with 0.1 % citric acid-70% EtOH. Then it was evaporated with large scale evaporation system. After adding dextrin to C3G concentration materials, we made pigment powder with freezing dryer.

Effects of nutrition education on nutrition-related knowledge, dietary habits, and nutrient intakes of alcoholic patients (영양교육이 알코올중독자의 영양지식, 식습관 및 영양섭취상태에 미치는 효과)

  • Kim, An Na;Lim, Hyeon-Sook
    • Journal of Nutrition and Health
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    • v.47 no.4
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    • pp.277-286
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    • 2014
  • Purpose: The aim of this study was to examine the characteristics of nutrition-related knowledge, dietary habits, and nutrient intakes of alcoholic patients and how nutrition education affects these nutritional behaviors. Methods: Subjects included 37 adult male alcoholic patients who were hospitalized. The nutrition education program consisted of five lessons over a five-week period. An each 80-minute nutrition education program per week was implemented for the alcoholic patients over a five-week period. Both before and after the implementation of nutrition education, their nutrition-related knowledge and dietary habits were assessed and nutrient intakes were investigated. Results: The subjects showed drinking habits of considerably high frequency, a large quantity of alcohol consumption, and preference for soju (a liquor) over beer. They had proper weight, height, and BMI, and came from relatively poor socioeconomic backgrounds with a low-level of self-rated health status and a comparatively high rate of suffering from disease. Mean score of their nutrition-related knowledge and dietary habits was quite low. They consumed less energy, dietary fiber, vitamin C, thiamin, riboflavin, folic acid, Ca, and K, but more Na compared to each Dietary Reference Intake (DRI). After implementing the nutrition education, mean score of nutrition-related knowledge and dietary habits showed significant improvement. In addition, the meeting rate of each DRI of several nutrients was increased significantly, including energy, carbohydrate, dietary fiber, vitamins A, C, and $B_6$, thiamin, riboflavin, niacin, pyridoxine, folic acid, Ca, P, K, Fe, and Zn, while that of Na decreased. Conclusion: The findings of this study indicate that alcoholic patients had various nutritional problems, such as lack of nutrition-related knowledge, bad dietary habits, and insufficient nutrient intakes, however, these problems can be positively modified by implementation of a relatively short-term nutrition education program.