• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.2B
• /
• pp.250-262
• /
• 2004

In IPv6 environment, the Internet Telephony, VoD(Video on Demand) and high capacity file exchange service will be more increased than IPv4. Therefore, the strict guarantee of QoS based on End-to-End and differentiated quality control schemes are simultaneously required. This paper proposes the flow control schemes on IPv6 network that the traffic is identified by flow and the QoS of multi-service is improved by QoS information in IPv6 hop-by-hop option header. The object of flow control includes not only non-default QoS traffic, which uses the flow label, but also best-effort or encrypted traffic. Therefore, the guarantee of real-time service is strengthened and the flow, which abuses unnecessarily the network resources, is effectively controlled. Also, this paper proposes the mapping scheme between the flow and MPLS by reflecting the minimum change of the existed network resource and the status of backbone network of ISP(Internet Service Provider). In the simulation result, It is shown that the proposed scheme is effective in the side of QoS on real-time services and utilization of backbone resources.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.6
• /
• pp.2447-2451
• /
• 2014

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.11C
• /
• pp.963-969
• /
• 2008

This paper presents 6bit 100MHz Interpolation Flash Analog-to-Digital Converter, which can be applied to the Receiver of Wireless Tele-communication System. The 6bit 100MHz Flash Analog-to-Digital Converter simplifies and integrates S-R latch which multiplies as the resolution increases. Whereas the conventional NAND based S-R latch needed eight MOS transistors, this Converter was designed with only six, which makes the Dynamic Power Dissipation of the A/D Converter reduced up to 12.5%. The designed A/D Converter went through $0.18{\mu}m$ CMOS n-well 1-poly 6-metal process to be a final product, and the final product has shown 282mW of power dissipation with 1.8V of Supply Voltage, 100MHz of conversion rate. And 35.027dBc, 31.253dB SFDR and 4.8bits, 4.2bits ENOB with 12.5MHz, 50MHz of each input frequency.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.6
• /
• pp.539-545
• /
• 2004

The purpose of this study was to locate the quantitative trait loci (QTL) associated with quality properties in the recombinant inbred lines derived from the 'Milyang 23' and 'Gihobyeo' cross. Four quality-related traits; protein content, amylose content, fat acid content and sensory value were measured. Eight QTLs for protein content were detected on chromosomes 1 (two loci), 3, 6, 7 and 8 (three loci), each accounting for $6.0\%\~15.2\%$ of the phenotypic variation. Three QTLs for amylose content were detected on chromosomes 6 and 7 (two loci), each explaining from $7.3\%\;to\;24.4\%$ of the phenotypic variation. Six QTLs for fat acid content were detected on chromosomes 2 (two loci), 3, 6 (two loci) and 7, each explaining form $5.5\%\;to\;14.0\%$ of the phenotypic variation. Six QTLs for sensory value were detected on chromosomes 2, 6, 7(two loci) and 8 (two loci), each accounting for $5.5\%\~10.3\%$ of the phenotypic variation.

• Journal of Animal Science and Technology
• /
• v.47 no.4
• /
• pp.615-624
• /
• 2005

The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.2
• /
• pp.685-689
• /
• 2013

Objective: To study the effects of down-regulation of HDAC6 expression on proliferation, cell cycling and migration of esophageal squamous cell carcinoma (ESCC) cells and related molecular mechanisms. Methods: ESCC cell line EC9706 cells were randomly divided into untreated (with no transfection), control siRNA (transfected with control siRNA) and HDAC6 siRNA (transfected with HDAC6 small interfering RNA) groups. Effects of HDAC6 siRNA interference on expression of HDAC6 mRNA and protein in EC9706 cells were investigated by semi-quantitative RT-PCR, Western blotting and immunocytochemistry methods. Effects of down-regulation of HDAC6 expression on cell proliferation, cell cycle, and cell migration were studied using a CCK-8 kit, flow cytometry and Boyden chambers, respectively. Changes of mRNA and protein expression levels of cell cycle related factor (p21) and cell migration related factor (E-cadherin) were investigated by semi-quantitative RT-PCR and Western blotting methods. Results: After transfection of HDAC6 siRNA, the expression of HDAC6 mRNA and protein in EC9706 cells was significantly downregulated. In the HDAC6 siRNA group, cell proliferation was markedly inhibited, the percentage of cells in G0/G1 phase evidently increased and the percentage of cells in S phase decreased, and the number of migrating cells significantly and obviously decreased. The mRNA and protein expression levels of p21 and E-cadherin in the HDAC6 siRNA group were significantly higher than those in the untreated group and the control siRNA group, respectively. Conclusions: HDAC6 siRNA can effectively downregulate the expression of HDAC6 mRNA and protein in EC9706 cells. Down-regulation of HDAC6 expression can obviously inhibit cell proliferation, arrest cell cycling in the G0/G1 phase and reduce cell migration. The latter two functions may be closely related with the elevation of mRNA and protein expression of p21 and E-cadherin.

• Journal of Families and Better Life
• /
• v.26 no.5
• /
• pp.155-163
• /
• 2008

This study examined the relationships between children's stress about obsesity and depression. In order to expand the study on the relationships, special attention was given to the possible moderator effects of child's ego-resiliency. The subjects were 494 4th and 6th graders residing in Seoul. It was found that 6th grade children's stress about obesity and depression was higher more than 4th graders in every boys and girls. Otherwise, 4th grade children's ego resiliency more higher in girls, 6th grade children's ego resiliency more higher in boysn. Children's stress about obesity was significantly related with the levels of childre's depression. The results indicated that children's higher obesity stress was related to higher levels of children's depression. Children's ego resiliency were found to be a significant moderator of relationship between children's obesity stress and depression. These findings indicated that children's ego resiliency could fuction as a protecting factor for children who perceive obesity stress.

• Korean journal of food and cookery science
• /
• v.30 no.4
• /
• pp.428-434
• /
• 2014

Physicochemical properties, sensory characteristics, and consumer acceptability of cookies produced with oat and barley powder were evaluated. Oat and barley powder was incorporated into cookie dough at 6 levels: oat : barley = 100:0 (S1), 80:20 (S2), 60:40 (S3), 40:60 (S4), 20:80 (S5), 0:100 (S6) and control. pH of the cookie dough was lowest in the S6 group and highest in the control, S1, S2, and S3 groups. There were no significant differences in density. The spread ratio and loss rate of cookies increased significantly with an increase in content of barley powder. The leavening rate of the S1 cookie group was the lowest. With regard to color, the a-values and b-values of cookies increased with a rise in content of oat powder, and the L-value increased with an increase in barley powder content. Analysis of cookie texture showed that hardness was highest in the S6 group with barley and lowest in the S1 group, but there was no significant difference between the control group and other experimental groups. In the sensory evaluation of cookies, there was no significant difference between the control and experimental groups in roasted nuttiness and sweetness. In overall acceptance of cookies, S6 was the least acceptable and S1, S2, and S3 were the most acceptable. Results suggest that oat powder is a suitable ingredient for increasing the functionality of cookies and consumer acceptability.

• Proceedings of the Korean Information Science Society Conference
• /
• 2007.06d
• /
• pp.327-332
• /
• 2007

현재 전 세계는 IPv4 주소 자원의 고갈에 대비한 IPv6의 도입을 위해 국가 차원의 전략적인 노력을 기울이고 있으며, 이에 따라 IPv4망에서 IPv6망으로의 안정적인 전이를 위한 상호 운영기술이 지속적으로 개발되고 있다. 이러한 기술을 적용하기 위한 다양한 응용 프로그램의 운영을 위해서 IPv6 뿐만 아니라 IPv6 기반의 Recursive DNS 기술도 그 핵심적인 요소라 할 수 있다. 그렇지만 IPv6 및 IPv6 DNS를 구축과 동시에 사용자 PC의 OS에도 IPv6 및 IPv6 DNS가 설정되어야 한다. 그러나 사용자가 사용하는 운영체제의 종류가 다양하기 때문에 운영체제별 동작도 다르다. 따라서 IPv6 및 IPv6 Recursive DNS을 사용하기 위해서는 사용자 PC의 서로 상이한 운영체제별 IPv6 및 IPv6 DNS 동작을 파악해야만 한다. 이에 본 논문은 운영체제별 IPv6 및 IPv6 DNS 동작을 확인하고 IPv6 및 IPv6 DNS 구축시 고려해야 할 가이드라인을 제시한다.

• Bulletin of the Korean Chemical Society
• /
• v.20 no.6
• /
• pp.749-752
• /
• 1999