• Bulletin of the Korean Chemical Society
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• 제16권9호
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• pp.835-839
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• 1995

Mer,trans-Re(≡NC6H5)(PPh3)2Cl3, Ⅰ, reacted with trimethylphosphine to give a mixture of two stereoisomers, mer,trans-Re(≡NC6H5)(PMe3)2Cl3,Ⅱ, and fac,cis-Re(≡NC6H5)(PMe3)2Cl3, Ⅲ. These compounds could also be prepared from the reaction of Re(≡NC6H5)(PMe3)(PPh3)Cl3 with trimethylphosphine. In both reactions the mer,trans-isomer is a major product. The products have been characterized by NMR, elemental analysis, and X-ray crystallography. Crystal data for Ⅱ: monoclinic space group P21, a=10.053(1) Å, b=10.844(1) Å, c=10.058(2) Å, β=113.45(2)°, Z=2, R(wR2)=0.0348 (0.0894). Crystal data for Ⅲ: monoclinic space group P21/n, a=7.183(2) Å, b=16.983(4) Å, c=15.543(4) Å, β=90.38(2)°, Z=4, R(wR2)=0.0603 (0.1484).

• The Korean Journal of Physiology and Pharmacology
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• 제6권2호
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• pp.127-130
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• 2002

The present study was aimed to investigate whether the adriamycin-induced nephrosis is associated with an altered regulation of local renin-angiotensin system (RAS) in the kidney. Rats were subjected to a single injection of adriamycin (2 mg/kg body weight, IV) and kept for 6 weeks to allow the development of nephrosis. They were then divided into two groups, and supplied with and without cilazapril, an angiotensin converting enzyme (ACE) inhibitor, in drinking water (100 mg/l) for additional 6 weeks. Another group without adriamycin-treatment served as control. The mRNA expression of renin, ACE, type 1 and type 2 angiotensin II receptors (AT1R, AT2R), and transforming growth factor $(TGF)-{\beta}1$ was determined in the cortex of the kidney by reverse transcription-polymerase chain reaction. Adriamycin treatment resulted in heavy proteinuria. Accordingly, the mRNA expression of renin, ACE, and AT1R was increased in the renal cortex, while that of AT2R was decreased. Co-treatment with cilazapril attenuated the degree of proteinuria. While not affecting the altered expression of renin, cilazapril decreased the expression of ACE to the control level. Cilazapril further increased the expression of AT1R, while it restored the decreased expression of AT2R. The expression of $(TGF)-{\beta}1$ was increased by the treatment with adriamycin, which was abolished by cilazapril. An altered expression of local RAS components may be causally related with the development of adriamycin-induced nephrosis, in which AT1R is for and AT2R is against the development of nephrosis.

• Journal of Sericultural and Entomological Science
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• 제9권
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• pp.43-47
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• 1969

This experiment was carried out to investigate the effect of gamma ray on the eggs of later stage in late fall rearing season. The results are summarized as follows: 1. The duration of larval stage was simmilar to that of control in any dose. The silkworm growth was uniform in does of 4,000 gamma ray, but in does of 8,000r 16000r was irregular. There were not malformed silkworms but many small silkworm. 2. Mortality of silkworms was very high 8,000r and over; virus diseases of 87.6 per cent, flacherie of 5.9 per cent and complication of 6.5 per cent. 3. The more the dose of gamma ray became, the lighter the weight full grown silkworm and matured silkworm became. 4. The uniformness of cocoons was registered in dose of 2,000r and the cocoons were irregular or small in dose of 16,000r

• Journal of Korean Home Economics Education Association
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• 제5권1호
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• pp.97-111
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• 1993

본 연구는 국민학교 실과 과목의 식품 영양 단원에 관한 교사, 학생의 인지 및 학생의 지식, 태도, 기능 평가를 위하여 71개 인천시내 공립 국민학교 4,5,6학년 교사 510명과 12개교학생 1,754명을 대상으로 설문조사를 통한 내용을 분석한 것으로 연구의 목적은 1)실과의 정해진 교육목표에 관한 교사의 인지정도 규명 2)식품, 영양 단원에서 교사가 중요하다고 인지하는 내용과 가르치기에 어려움을 느끼는 내용 규명 3)교사가 인지하는 식품영양 단원학습을 증진하기 위한 요소 4) 학생들이 흥미를 느끼는 내용 5)본 단원 학습 후 학생의 지식, 태도, 기능 정도 6)지식, 태도 기능간의 상호 상관관계 규명 등이며 그 연구결과는 다음과 같다. 1. 교사들이 인지하는 실과의 정해진 교육목표는 거의 모두가 중요한 것으로 나타났다. 2. 교사들이 인지하는 식품 영양 단원의 중요한 내용은 영양과 질병과의 관계, 식품위생, 식품저장 등이며, 가장 중요하지 않은 내용으로는 영양 필요량이었다. 또한, 지도에 어려움을 느끼는 내용은 영양 필요량, 조리준비, 식품구성/함유량 등이며 어려움을 느끼지 않는 내용은 영양과 질병과의 관계, 식품위생, 식품저장 등이었다. 3. 교사가 인지하는 단원 학습 증진을 위한 긍정적 요소는 적합한 실습설비와 자료, 시청가자료등이었고 부정적 요소는 남교사, 실험 실습이 필요한 부분은 숙제로 미루는 것 등이었다. 4. 학생들이 흥미를 느끼는 내용으로는 간단한 음식 만들기, 영양소의 기능, 영양문제 등이었고, 쥬스나 차 준비, 조리연료, 부엌용구 사용법 등은 흥미를 느끼지 못하는 내용이었다. 5. 학생의 학업성취 중 지식은 4학년이 식품위생, 기초식품군이, 5학년은 알맞은 조리용구, 조리원리, 6학년은 여가활용의 뜻, 올바른 예절 등이 90% 이상 바르게 답하였고 5학년의 삶은 달걀의 화학적 변화, 감자의 주된 영양 등은 60%이하였다. 6 태도 테스트 결과는 5학년이 4,6학년보다 약간 높은정도(Mn=3.653) 였으나 기능테스트에서는 4학년이 5,6학년보다 약간 낮게 (Mn=3.0) 나타났다(구체적인 것은 본문 참조). 7, 4, 5, 6학년 모두 지식, 태도 기능 간에 정적 상관을 나타내었는데 특히 태도와 기능 사이에는 4학년 r=.7166, 5학년 r=.5175, 6학년 r=.6796을 나타내었고 지시과 태도(4학년 r=.1546, 5학년 r=.0872, 6학년 r=.2192)와 지식과 기능사이에서는 (4학년 r=.1631, 5학년 r=.0585, 6학년 r=.2521)태도와 기능간보다 약한 상관관계를 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3961-3968
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• 2015

Background: Variants of human papillomavirus (HPV) show more oncogenicity than do prototypes. The HPV16 Asian variant (HPV16As) plays a major role in cervical cancer of Asian populations. Some amino acid changes in the E6 protein of HPV16 variants affect E6 functions such as p53 interaction and host immune surveillance. This study aimed to investigate activities of HPV16As E6 protein on modulation of expression of miRNA-21 as well as interferon regulatory factors (IRFs) 1, 3, 7 and c-fos. Materials and Methods: Vectors expressing E6 protein of HPV16As (E6D25E) or HPV16 prototype (E6Pro) were constructed and transfected into C33A cells. HCK1T cells expressing E6D25E or E6Pro were established by transducing retrovirus-containing E6D25E or 16E6Pro. The E6AP-binding activity of E6 and proliferation of the transfected C33A cells were determined. MiR-21 and mRNA of interesting genes were detected in the transfected C33A cells and/or the HCK1T cells, with or without treatment by culture medium from HeLa cells (HeLa-CM). Results: E6D25E showed binding activity with E6AP similar to that of E6Pro. Interestingly, E6D25E showed a higher activity of miR-21 induction than did E6Pro in C33A cells expressing E6 protein. This result was similar to the HCK1T cells expressing E6 protein, with HeLa-CM treatment. The miR-21 up-regulation significantly corresponded to its target expression. Different levels of expression of IRFs were also observed in the HCK1T cells expressing E6 protein. Interestingly, when treated with HeLa-CM, IRFs 1, 3 and 7 as well as c-fos were significantly suppressed in the HCK1T cells expressing E6D25E, whereas those in the HCK1T cells expressing E6Pro were induced. A similar situation was seen for IFN-${\alpha}$ and IFN-${\beta}$. Conclusions: E6D25E of the HPV16As variant differed from the E6 prototype in its activities on epigenetic modulation and immune surveillance and this might be a key factor for the important role of this variant in cervical cancer progression.

• Microbiology and Biotechnology Letters
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• 제26권2호
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• pp.89-95
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• 1998

Rhodopseudomonas sphaeroides K7 and E15-1 produced hydrogen from glucose rapidly for the first 24 hrs of culture under the anaerobic and photosynthetic conditions and then ceased the hydrogen production because of the accumulation of organic acids such as acetic acid and formic acid in the culture broth, decreasing the pH to 4.2-4.5. Only 43% and 73% of glucose in the culture were consumed even after 6 days of incubation by R. sphaeroides K7 and E15-1, respectively. The hydrogen production and glucose consumption, however, were substantially increased when the pH of the culture was adjusted to 6.8-7.0: Hydrogen production continues even after 10 days of culture and glucose was consumed completely after 2.5 and 4.5 days by R. sphaeroides K7 and E15-1, respectively, Furthermore, the bacteriochlorophyll contents in R. sphaeroides K7 and E15-1 were increased by 44 and 9 folds and the cell concentrations by 10 and 2.5 folds, respectively, after 7 days of culture. R. sphaeroides K7 and E15-1 also produced hydrogen from acetic, lactic, butyric and malic acids under the anaerobic and photosynthetic conditions even though the amounts of hydrogen produced were lower than that from glucose. The results of this experiment indicate that under the anaerobic and synthetic conditions R. sphaeroides K7 and E15-1 might use the NADH oxidation mediated by ferredoxin and hydrogenase to evolve hydrogen from glucose for the first 24 hrs and then the organic acids produced were used as electron donners for the production of hydrogen in the nitrogen-limited condition.

• Proceedings of the KSRS Conference
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.651-654
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• 2006

Infrared channels of newly launched Japanese geostationary satellite, MTSAT-1R are compared with well calibrated MODIS/Terra infrared measurements at 3.7, 6.7, 11, 12 ${\mu}m$ bands. There are four steps in this intercalibration method: 1) data collection, 2) spectral response function correction, 3) data collocation, and 4) calculation of mean bias and conversion coefficients. In order to minimize the navigation error of MTSAT-1R, comparisons are made over the area in which the viewing angle of MTSAT-1R is less than 50$^{\circ}$. The calibration method was tested for August 2005 and within the 40$^{\circ}N$-40$^{\circ}S$, 100$^{\circ}$E-180$^{\circ}$E domain. The differences of spectral response functions were corrected through radiative transfer model simulation. Constructing collocated data differences in viewing geometry, observation time and space were taken into account. In order to avoid the radiance variation induced by cloud presence, clear-sky targets are selected as intercalibration target. The mean biases of 11, 12, 6.7, and 3.7 ${\mu}m$ bands are about -0.16, 0.36, 1.31, and -6.69 K, suggesting that accuracies of 3.7 ${\mu}m$ is questionable while other channels are comparable to MODIS

• Journal of the Korean Applied Science and Technology
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• 제36권3호
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• pp.853-865
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• 2019

In this study, the skin permeability was measured by adding skin penetrating peptides, arginine oligomers R4(tetra-D-arginine), R6(hexa-D-arginine) to little skin-permeable wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK liposomes, and the results were analyzed by the following six cases. (1) In cases where only wrinkle improvement peptides GHK, GHK-Cu, and Pal-GHK were contained liposomes; the final cumulative permeations in 24 hours were 6.05%, 7.4%, and 8.83% respectively. (2) In cases where arginine oligomers R4, R6 were added to GHK liposomes; the final cumulative permeations in 24 hours were 13.63% and 7.68%. (3) In cases where R4, R6 were added to GHK-Cu liposomes; the final cumulative permeations in 24 hours were 15.46% and 8.64%. (4) In cases where R4, R6 were added to Pal-GHK liposomes; the final cumulative permeations in 24 hours were 16.9% and 10.67%. (5) In cases where R4 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 13.63%, 15.46%, and 16.9% respectively. (6) In cases where R6 were added to GHK, GHK-Cu, and Pal-GHK liposomes; the final cumulative permeations in 24 hours were 7.68%, 8.64%, and 10.67% respectively. This experiment showed that skin absorption of GHK was increased by copper ion (Cu2+) and palmitic acid and skin absorption of wrinkle improvement peptides was enhanced by cell penetrating peptides, and R4 showed higher effect than R6 in GHK, GHK-Cu and Pal-GHK. Through this process, we propose broad use and application in wrinkle improvement functional cosmetics by presenting the optimal conditions for increasing skin absorption of GHK, GHK-Cu, thus maximizing its efficacy.

• Journal of Nutrition and Health
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• 제36권7호
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• pp.743-748
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• 2003

This study was longitudinally conducted to evaluate vitamin A intake of exclusively breast-fed infants compared with the Recommended Dietary Allowances (RDA) for Korean infants. Twenty-seven Korean lactating women and their infants during the first 6 months of lactation in Cheongju and Anseong areas were participated. Retinol and $\beta$-carotene contents in the milk were determined using HPLC and also the milk consumption of the infants was measured by the test-weighing methods. Vitamin A (retinol and $\beta$-carotene) contents of the milk were 65.7, 57.2, 48.1, 43.9, 38.2, 38.7 and 44.0 R.E./100 ml, and vitamin A intakes of the breast-fed infants were 361, 402, 348, 331, 304, 305 and 322 R.E./day at 0.5, 1, 2, 3, 4, 5 and 6 month of lactation, respectively. The average intake of vitamin A was 339.1 R.E./day and the percentage to RDA was 96.9% during 6 months. Vitamin A intakes per body weight of the breast-fed infants were 96.7, 88.3, 62.1, 50.0, 41.8, 39.3 and 39.6 R.E./kg/day at 0.5, 1, 2, 3, 4, 5 and 6 month. The body weight increased normally from 3.4 $\pm$ 0.5 kg at birth during lactation. It is suggested that the breast-fed infants in Cheongju and Anseong areas consumed adequately vitamin A from the milk compared with RDA for Korean infants.

• Korean Journal of Veterinary Research
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• 제26권1호
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• pp.69-77
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• 1986

The purpose of this study was the examination for presence of K99 antigen (K99), enterotoxigenicity, 0-groups, colicin and antibiotic susceptibility among E. coil isolated from calves and cows. A total of 49(18.7%) among 262 strains, isolated from 30(26.5%) out of 113 calves and cows, possesed K99, and thirty three of 49 $K99^+$ strains produced ST. Of the strains of diarrheal calf origin which less than 15 days old, a high correlation was observed between enterotoxigenic ability and K99: 92.3% of the $K99^+$ strains produced heat stable enterotoxin(ST). In O group typing of 33 $ST^+$ strains, they belonged to O20(48.4%), O8(9.1%), O9(6.1%), O139(6.1%), O149(6.1%), O101(3.0%), O115(3.0%), except six which were untypable or autoagglutinable. Of 262 E. coil isolates, 30 strains(13.3%) produced colicin and K99 were detected in 6 strains. One hundred eighty eight strains(71.8%) of 262 E. coil isolates were resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, tetracycline, alone or in combination thereof. One hundred and fourteen(60.6%) out of 188 drug resistance strains carried R factor($R^+$) which were transferable to the recipients by conjugation. Sixty five $R^+$ strains(57.0%) carried thermo-sensitive R plasmid.