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Speciation of Viola albida var. takahashii based on both artificial and natural hybridization between V. albida var. albida and V. albida var. chaerophylloides

  • Songhee AHN;Sung Soo WHANG
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.226-234
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    • 2022
  • The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F1, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F1 bore also both chasmogamous and cleistogamous flowers. F1 seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F2, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F2 leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F2 takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.

The Effects of Oral Care on Children by Parents (부모의 아동에 대한 구강관리가 아동의 구강관리행위에 미치는 영향)

  • Kang, Hyun Suk;Kim, Kyung Hee;Chung, Hae Kyung
    • Journal of the Korean Society of School Health
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    • v.13 no.2
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    • pp.319-329
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    • 2000
  • This study suggestes that the improvement of oral care through on investigation of the effects of oral care on children by parents. This investigation was done on Sep. 29 1999 through Oct. 8 examining 376 of elementary, 1grade, 2grade, 3grade students in Kyunggi-Do. The content of the questions was analzed with a t-test and a one-way ANOVA; the relation to the oral care on children by parents with oral care behavior was analyzed by a Pearson's Correlation Coefficient. The results are as follow; 1) Statistically women were higher(F=-2.675, p<0.01). The average of the oral care of children was 2.34, man averaged 2.29, woman are raged 2.32. Statistically women were also higher(F=4.682, p<0.052). 2) The more educatior the mother had higher, the higher the grade. Statistically there was a difference(F=6.756, p<0.01. The oral care of children above Uni graduate(M=2.38), high (M=2:23), middle graduate(M=2.Z3). Statistically, the higher the academic degree, the more optimistic(F=4.114, p<0.05). 3) In the oral care on children by parents, when the conversation with the family was often 2.17, there was little difference(F=7.343, p<0.01). In the oral care of children was 2.45, there was little difference(F=7.343. p<0.01). As a result, the conversation with family affects the behavior. 4) In the oral care of children, if the food that they like was fruits, vegetables, and fresh food, the average was 2.37, very high. There was little difference in other cases(F=-3.179, p<0.05). We can conclude that fresh food affects the oral care of children. 5) In the oral care on children by parents, when the course of knowledge on the oral care was viewed by a grandfather(M=2.02), grandmother(M=2.05), father(M=2.14), mother(M=2.08). There was little difference(F=2.666, p<0.05). 6) In oral care, if the level of income was viewed high level(M=2.42), middle(M=2.34), 10w(M=1.88), there was a little difference(F=3.300, p<0.05). 7) The relation of the oral care on children by parents and the oral care was normal. In other words, the more management by parents, the more management by children.(p<0.01) Therefore in this study, the oral care on children by parents effect the oral care behavior of children. To improve the mouth health of children, the parents's interest in their children must be constant. Also, it is needed to develope an education program where parents and children can be educated.

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Effect of Levels of Total Neutral Detergent Fiber and Forage-derived Neutral Detergent Fiber on Feed Intake and Milk production in Holstein Dairy Cows (홀스타인 착유우에서 중성세제불용섬유소의 수준과 조사료유래 중성세제불용섬유소의 수준이 사료섭취량 및 유생산성에 미치는 영향)

  • Lee, Do Hyung;Kwon, Chan Ho;Kim, Eun Joong;Kim, Hyun-Jin;Kim, Gyeom-Heon;Kim, Soo-Ki
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.4
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    • pp.304-312
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    • 2013
  • This study was conducted to investigate the effect of total neutral detergent fiber (NDF) and forage-derived NDF (fNDF) on nutrient intake and milk production in Holstein dairy cows. Thirty-six Holstein dairy cows at weeks 4 to 20 of lactation were included in this study. The cows were arranged in a randomized complete block design and assigned to one of six diets: 1) 38% NDF with 18% fNDF (HN-HF); 2) 38% NDF with 15% fNDF (HN-MF); 3) 38% with 12% fNDF (HN-LF); 4) 34% NDF with 18% fNDF (LN-HF); 5) 34% NDF with 15% fNDF (LN-MF); and 6) 34% with 12% fNDF (LN-LF). The cows were fed a total mixed ration twice daily (07:00 and 18:00 h) and given ad libitum access to feed and water throughout the experiment. Data on the intake of nutrients (DM, NEl, CP, NDF, fNDF, NFC), milk yield, composition, fat-corrected milk (FCM), fat and protein corrected milk (FPCM), and energy corrected milk (ECM) were analyzed. Lower dietary concentrations of total NDF or fNDF resulted in an increase in DM, NEl, and NFC intake (p<0.01), milk yield (p<0.05) and a lower milk fat concentration (p<0.01). The LN treatment showed no significant differences in milk fat concentration compared to the HN however, further reduction in fNDF decreased milk fat content (p<0.01). The lowest level of milk fat was observed in the LN-LF diet group. Changes in the dietary concentrations of NDF or fNDF did not affect the concentrations of milk protein or SNF (solid not fat), although the yield of milk protein and SNF increased with higher milk yield (p<0.01). The level of fNDF influenced the level of FCM (p=0.07), as well as FPCM and ECM yield (p<0.05). A highly positive correlation between fNDF intake and milk fat concentration was observed in animals with low NDF compared to those offered high NDF. A strong correlation was also observed between milk yield and low NDF intake compared to high NDF intake.

A New Early-Heading and High-Yielding Forage Winter Oat Cultivar, "Punghan" (내한 조숙 다수 추파 조사료용 귀리 신품종 "풍한")

  • Han, Ouk-Kyu;Park, Hyong-Ho;Heo, Hwa-Young;Park, Tae-Il;Seo, Jae-Hwan;Park, Ki-Hun;Kim, Jung-Gon;Ju, Jung-Il;Hong, Yun-Gi;Jeung, Jae-Hyun;Park, Nam-Geon
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.168-172
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    • 2009
  • 'Punghan' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2008. It was derived from a cross between 'Beltsville 61-150 (IT133501)' and $F_1$ between 'Sikyonggwiri' and 'PA202-210'. A oat cultivar from USA, 'Beltsville 61-150', has a high cold tolerance, while the $F_1$ between 'Sikyonggwiri' and 'PA202-210' has early heading and high yielding with large-size grain. Subsequent enerations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO99013-B-YB-31', was selected for earliness and good agronomic characteristics and designated as a line name of 'Gwiri 60'. The line 'Gwiri 60' was subsequently evaluated for winter hardiness, earliness, and forage yield in five locations, Yesan, Cheongwon, Iksan, Kimjae, and Jeju, from 2006 to 2008 and finally named as 'Punghan'. Over 3 years, the average forage dry matter yield of 'Punghan' harvested at milk-ripe stage was $14.5\;ton\;ha^{-1}$, compared with $13.6\;ton\;ha^{-1}$ of check cultivar 'Samhan'. 'Punghan' is about 2 days earlier in heading date than check cultivar. 'Punghan' is adapted primarily for winter planting use in the mid-southern regions of Korean peninsula.

Fabrication of Mesoporous Carbon Nanofibers for Electrical Double-Layer Capacitors (전기 이중층 커패시터용 메조 다공성 탄소 나노섬유의 제조)

  • Lee, Do-Young;An, Geon-Hyoung;Ahn, Hyo-Jin
    • Korean Journal of Materials Research
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    • v.27 no.11
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    • pp.617-623
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    • 2017
  • Mesoporous carbon nanofibers as electrode material for electrical double-layer capacitors(EDLCs) are fabricated using the electrospinning method and carbonization. Their morphologies, structures, chemical bonding states, porous structure, and electrochemical performance are investigated. The optimized mesoporous carbon nanofiber has a high sepecific surface area of $667m^2\;g^{-1}$, high average pore size of 6.3 nm, and high mesopore volume fraction of 80 %, as well as a unifom network structure consiting of a 1-D nanofiber stucture. The optimized mesoporous carbon nanofiber shows outstanding electrochemical performance with high specific capacitance of $87F\;g^{-1}$ at a current density of $0.1A\;g^{-1}$, high-rate performance ($72F\;g^{-1}$ at a current density of $20.0A\;g^{-1}$), and good cycling stability ($92F\;g^{-1}$ after 100 cycles). The improvement of the electrochemical performance via the combined effects of high specific surface area are due to the high mesopore volume fraction of the carbon nanofibers.

Implementation of Abalone Aquaculture Security System Based on the Expected Invading Route Analysis of a Thief;Focused on the Abalone Farm Field Located in the Jindo-Kun, Jeonranam-Do (어장도적 침입 경로분석에 기반한 전복 양식장 도적대응 시스템 구현에 관한 연구;전라남도 진도군 보전리 참전복 양식단지를 중심으로)

  • Yim, Jeong-Bin;Nam, Taek-Keun;Ahn, Young-Sup
    • Proceedings of KOSOMES biannual meeting
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    • 2007.11a
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    • pp.87-89
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    • 2007
  • 선행 연구개발한 집단감시 디지털 시스템(Group Digital Surveillance System for Fishery Safety and Security, GDSS- F2S)은 대단위 양식장에 침입하는 도적을 방어하기 위하여 레이더 추적정보와 적아식별 정보를 제공하는 시스템이다. 그러나 GDSS-F2S에서 제공하는 두 가지 정보만으로는 도적행위를 입증할 수 있는 법적 증거자료로 미흡하다. 본 논문에서는 이러한 문제점 해결방안의 하나로 CCD 카메라를 이용한 영상획득 장치를 개발하여 GDSS-F2S에 부가한 연구내용을 기술한다. 영상획득 장치 개발에 앞서, 실험지역의 지리적인 특성을 고려한 도적침입 예상경로 분석과 도적행위 시나리오를 기반으로 유효한 대응수단을 검토한 결과, 영상획득 장치가 유효한 대응수단임을 확인하였다. 영상획득 장치는 저가이변서 성능이 우수한 0.0001 룩스의 초저조도 CCD 카메라와 부가장치를 이용하여 개발하였다. 6개월 이상의 장기 현장실험을 통하여 본 연구에서 개발한 시스템을 평가한 결과, 주간은 물론 1 미터 앞도 식별할 수 없는 야간에도 차량의 행동과 번호판 및 사람의 행동과 인상착의 등의 영상정보를 확보할 수 있었다.

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Improvement of PMOLED life time using Mg-Zn-F thin film passivation

  • Kim, Jae-Hyun;Seo, Jun-Seon;Hong, Seok-Min;Kang, Byoung-Ho;Kim, Do-Eok;Lee, Sung-Youp;Shin, Byong-Wook;Lee, Hyeong-Rag;Kim, Hak-Rin;Kang, Shin-Won
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.794-797
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    • 2009
  • We manufactured a novel gas permeation thin film passivation by using inorganic Mg-Zn-F target which has better optical characteristics and high electronegativity. We fabricated targets in various composition ratio and formed about 200nm which is limited thickness of the flexible display. Applied to PLED device, the target which composed of $MgF_2$ and Zn at the ratio of 4:6, WVTR was reached the measurement limit of the equipment, $10^{-3}g/m^2{\cdot}day$ and the life time was increased 25 times better than PLED device which is non-passivation.

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First Report of Fruit Rot Caused by Fusarium decemcellulare in Apples in Korea

  • Lee, Seung-Yeol;Park, Su-Jin;Lee, Jae-Jin;Back, Chang-Gi;Ten, Leonid N.;Kang, In-Kyu;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.54-62
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    • 2017
  • In 2014, abnormal brown spots were observed on Hongro apples in fields in Gyeongsangbuk-do Province and during low-temperature storage. The spots were round, blight brown, and different from the symptoms of previously reported apple diseases. A fungal pathogen was isolated and cultured on potato dextrose agar, and it was morphologically similar to Fusarium decemcellulare. A pathogenicity test showed the same brown spots on both wounded and unwounded Hongro and Fuji apple cultivars. RPB1 and RPB2 sequences of F. decemcellulare KNU-GC01 matched with those of F. decemcellulare NRRL 13412 (98.3% and 97.6% similarities, respectively); both strains clustered together in the phylogenetic tree, indicating their close relationship at the species level. Therefore, F. decemcellulare is a newly reported pathogen that causes brown spots on apples in Korea.

Enhancement of NK Cytotoxicity, Antimetastasis and Elongation Effect of Survival Time in B16-F10 Melanoma Cells by Oregonin

  • Joo, Seong-Soo;Kim, Min-Soo;Oh, Won-Sik;Lee, Do-Ik
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.493-499
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    • 2002
  • We investigated the antitumor activity of oregonin, a diarylheptanoid derivative purified from Alnus hirsuta Turcz, Betulaceae. Oregonin is a potential novel immunomodulator, which augments the activation of natural killer (NK) cells, and thereby leads to a powerful antitumor activity. To evaluate the cytotoxicity of oregonin against tumor cells, we examined the effectiveness of NK cells and determined that oregonin could increase NK cell cytotoxicity. This was confirmed by MTT assay. In addition, the survival time of C57BL/6 mice were measured by inoculating 816-F10 melanoma cells to mice via intra muscular (i.m.) injection. Oregonin treatment after 10 hours of inoculation at 10 mg/kg dosage showed a significant extension of survival time by up to 51.32%, when compared to the control group. Moreover, oregonin significantly reduced the incidence of pulmonary metastasis, which may be developed from 816-F10 melanoma cells. These findings suggest that oregon in may be classified as a new and novel immunomodulator due to its potential antitumor activity.

The Characteristics of pulverized Powder by Automatic Tablet Crusher

  • Doh, Seong-Tak;Kim, Sung-Hwan;Lee, Do-Young;Kwon, Oh-Ju
    • Biomedical Science Letters
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    • v.8 no.2
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    • pp.101-106
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    • 2002
  • The purpose of this study was to test the capacity of automatic tablet crusher. The Automatic tablet crusher not only saved crushing time, but also removed floating fine powders that was occurred during crushing and caused allergy. The characteristics of powder produced by this crusher were as follows: First, to meet the condition as powder after pulverizing 5∼10 g tablet it took 6 to 12 seconds crushing time. But six seconds would do to get powder f3r oral administration. Second, when the quantity of medicine was measured by HPLC, powder from automatic crusher showed higher than that from pestle. HPLC chromatogram indicated that there was no change of medicine in the process of pulverization.

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