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The Evaluation of UV-induced Mutation of the Microalgae, Chlorella vulgaris in Mass Production Systems (자외선에 의해 유도된 Chlorella vulgaris 돌연변이 균주의 대량 생산 시스템에서의 평가)

  • Choi, Tae-O;Kim, Kyong-Ho;Kim, Gun-Do;Choi, Tae-Jin;Jeon, Young Jae
    • Journal of Life Science
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    • v.27 no.10
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    • pp.1137-1144
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    • 2017
  • The microalgae Chlorella vulgaris has been considered an important alternative resource for biodiesel production. However, its industrial-scale production has been constrained by the low productivity of the biomass and lipid. To overcome this problem, we isolated and characterized a potentially economical oleaginous strain of C. vulgaris via the random mutagenesis technique using UV irradiation. Two types of mass production systems were compared for their yield of biomass and lipid content. Among the several putatively oleaginous strains that were isolated, the particular mutant strain designated as UBM1-10 in the laboratory showed an approximately 1.5-fold higher cell yield and lipid content than those from the wild type. Based on these results, UBM1-10 was selected and cultivated under outdoor conditions using two different types of reactors, a tubular-type photobioreactor (TBPR) and an open pond-type reactor (OPR). The results indicated that the mutant strain cultivated in the TBPR showed more than 5 times higher cell concentrations ($2.6g\;l^{-1}$) as compared to that from the strain cultured in the OPR ($0.5g\;l^{-1}$). After the mass cultivation, the cells of UBM1-10 and the parental strain were further investigated for crude lipid content and composition. The results indicate a 3-fold higher crude lipid content from UBM1-10 (0.3%, w/w) as compared to that from the parent strain (0.1% w/w). Therefore, this study demonstrated that the economic potential of C. vulgaris as a biodiesel production resource can be increased with the use of a photoreactor type as well as the strategic mutant isolation technique.

Study on Plans for the Establishment of Strategy on Climate Change Adaptation in Korea (국가 기후변화 적응 전략 수립 방안에 관한 연구)

  • Kwon, Won-Tae;Baek, Hee-Jeong;Choi, Kyung-Cheol;Chung, Hyo-Sang
    • Atmosphere
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    • v.15 no.4
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    • pp.213-227
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    • 2005
  • The global mean surface temperature has already increased by $0.6{\pm}0.2^{\circ}C$ over the last century, and warming in Korea is approximately twice as large as the global average. The Intergovernmental Panel on Climate Change (IPCC) has concluded that the majority of warming over the past 50 years could be attributed to human activities (IPCC, 2001a). In addition, the global surface temperature is expected to increase by 1.4 to $5.8^{\circ}C$ depending on the greenhouse gas emission scenarios during the $21^{st}$ century.Climate change resulting from increased greenhouse gas concentrations has the potential to harm societies and ecosystems. Reductions in emissions of greenhouse gases and their concentration in the atmosphere will reduce the degree and likelihood of significant adverse conditions due to the anticipated climate change. Mitigation policy has generally been the primary focus of public attention and policy efforts on climate change. However, some degree of climate change is inevitable due to the combination of continued increases in emissions and the inertia of the global climate system. Adaptation actions and strategies are needed for a complementary approach to mitigation. The United Nations Framework Convention on Climate Change (UNFCCC) currently addresses vulnerability and adaptation in the context of climate change negotiations and in future adaptation may be an important element of work under the Kyoto Protocol. There are several on-going programs to develop effective adaptation strategies and their implementation. But in general, many other countries are still on an initiating stage. The climate change science programs of the United States, Japan, England, and Germany are initiated to understand the current status of climate change science and adaptation researches in the developed countries. In this study, we propose the improvement on systems in policy and research aspects to effectively perform the necessary functions for development of nation-wide adaptation measures and their implementation. In policy aspect, the Korean Panel on Climate Change (KPCC) is introduced as a coordinating mechanism between government organizations related with climate change science, impact assessment and adaptation. Also in research aspect, there is a strong consensus on the need for construction of a national network on climate change research as trans-disciplinary research network.

Effects of Communication Skill on Interpersonal Satisfaction and Subjective Happiness among College Students (대학생의 의사소통 기술이 대인관계 만족도 및 주관적 행복감에 미치는 영향)

  • Park, Mijeong;Oh, Doonam
    • The Journal of the Korea Contents Association
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    • v.17 no.11
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    • pp.423-434
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    • 2017
  • This study is a descriptive survey research to investigate the effect of communication skills of college students on interpersonal satisfaction and subjective happiness. Data were collected using structured questionnaires from 222 students attending two universities located in C and S cities between June 1 and June 10, 2016, and were analyzed using t-test, One-way ANOVA, $Scheff{\acute{e}}$ test, Pearson's correlation coefficient, and hierarchical multiple regression, etc. The results of the study are as follows. The communication skills of the subjects were found to be 2.13 out of 1~5 points, and it was found that there was a significant difference according to grade, perceived home economy level, and leisure activities. Second, communication skills showed a positive correlation with interpersonal satisfaction and subjective happiness. Third, in relation to interpersonal satisfaction, the explanatory power of sex, grade, academic achievement, perceived family economic level and leisure activity was 26.7%, and communication skills further explained 18.4% of interpersonal satisfaction. Fourth, in relation to subjective happiness, the explanatory power of subjective gender, academic achievement, perceived family economic level and leisure activity was 33.0%, and communication skills further explained only 0.3% of subjective happiness. The results of this study suggest that communication-related education can be used as a major intervention strategy in the development of programs to improve interpersonal satisfaction and happiness of college students.

핵융합용 초전도선재의 크롬도금기술

  • Park, Pyeong-Ryeol
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2012.11a
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    • pp.32-32
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    • 2012
  • 화석연료의 남용으로 지구 온난화가 심화되어 환경과 생태계변화가 가속화되고 있고, 급속한 산업의 발달과 인류 삶의 질 향상에 따른 에너지 수요가 급증하고 있는 실정에 있으며, 일본 후쿠시마 원전사태로 원자력 에너지의 위험성으로 지구 인류환경은 심각한 국면을 맞이 하고 있어 대체 에너지의 하나로 핵융합 에너지 필요성이 증대되고 있다. 핵융합 에너지 연구 개발은 우리나라에서 KSTAR가 1997년부터 건설하기 시작하여 지난 2007년에 완공되어 지금 운용 중에 있고, 국제적으로 미국, EU, 러시아, 중국, 한국, 일본 인도가 참여하는 ITER 국제 공동프로젝트가 2004년에 건설을 시작하여 프랑스 카다라쉬에 실증 플란트를 건설 중에 있다. 이러한 핵융합 반응을 위해서는 10e-7이상의 높은 진공과 1억$^{\circ}C$ 이상에서 중수소와 삼중수소가 반응하여 발생하는 플라즈마를 제어 할 필요가 있으며, 초고온의 핵융합 플라즈마를 가두고 가동시키기 위해서는 약 12Tesla이상의 고자장 마그넷이 필요하다. 현재 ITER 실증 플란트에 사용되는 고자장 마그넷은 TF (Toroidal Field)코일과 CS (Central Field)코일에 Nb3Sn 초전도선재가 핵심부품으로 사용되고 있으며 ITER프로젝트에서는 약 850톤의 Nb3Sn 초전도선재가 사용될 전망이다. 그 중에서 일본 25%, EU, 러시아와 한국이 각각 20%, 중국7%, 미국8% 할당되어 참여국 대부분은 초전도선재를 전략적으로 공급하고 있다. 초전도 선재의 크롬도금은 1~2 마이크로미터 이하의 균일하고 얇은 도금 두께와 밀착성이 우수한 품질이 요구된다. 일반적으로 크롬도금은 산업현장에서 컨베이어 벨트 방식으로 장식이나, 내식성 및 내마모성의 특성을 필요로 할 때 사용되고 있으나, 선재에 크롬도금을 릴투릴(Reel to Reel) 방식으로 적용되는 경우는 세계적으로 아주 드물다. 핵융합 마그넷의 CICC(Conduct In Cable Conduit)도체를 만들기 위해서는 초전도선재를 이용, 3(Sc 2+OFC 1)$^*3^*5^*5^*6$형태로 연선과 케이블링을 하게 되며, 초전도 선재를 연선하고 케이블링을 할 때 크롬 도금층이 박리될 가능성이 있어 크롬도금 방법과 프로세스를 특별히 고안할 필요가 있다. ITER핵융합로 마그넷의 TF코일은 높이 14m, 폭 9m 최대자장 12Tesla, 최대전류 68kA, CICC도체 직경이 40mm로서 그 초전도 조관/도체 내부에 0.82mm 직경의 Nb3Sn 초전도 선재가 약 1350가닥으로 연선과 케이블링으로 구성되어 있다. ITER 핵융합 마그넷용 초전도 선재의 크롬도금은 마그넷 권선 후 Nb3Sn 초전도물질을 형성하기 위해서 $650^{\circ}C$에서 500시간 열처리를 실시하며 열처리 시 초전도 선재의 소선들 사이에 발생할 수 있는 소착을 방지하고, 초전도 선재에서 발생하는 AC loss를 감소시키며, Quench시 발생되는 열을 쉽게 확산시킴으로써, 초전도 마그넷의 열적 안정성(Thermal Stability) 향상과 필요에 따라서 소선간 통전울 가능하게 한다. 고려제강의 자회사인 케이에이티는 크롬도금 밀착성이 우수하고 도금두께 0.1마이크로 미터 이내 제어가 가능한 얇고 균일한 도금품질을 개발하여 한국형 핵융합 실험로인 KSTAR에 65톤 전량 공급하였고, 크롬 도금된 무산소동 선재 32톤과 초전도 선재 93톤을 전량 ITER 프로젝트에 공급하고 있으며, 2013년도 상반기에는 공급을 마무리할 예정이다.

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A Brief Efficiency and Clustering Measurement Way of Containerport by Using the Game Cross-efficiency Model (게임교차효율성모형을 이용한 컨테이너항만의 효율성 및 클러스터링 측정방법 소고)

  • Park, Rokyung
    • Journal of Korea Port Economic Association
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    • v.30 no.4
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    • pp.151-168
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    • 2014
  • The purpose of this paper is to show the brief efficiency and clustering measurement way by using the game cross-efficiency model which is newly introduced in this paper for 13 container ports during 3 years(2009, 2010, and 2013) with 3 input variables(depth, total area, and number of crane) and 1 output variable(container TEU). The main empirical results are as follows. First, the average rankings of game cross-efficiency model are Ningbo, Hongkong, Shanghai, Dubai, Singapore, Qingdao, Kaosiung, Busan, Tokyo, Incheon, Nagoya, Manila, Gwangyang ports in order. Second, according to ANOVA analysis, three models show the similar results in terms of the efficiency rankings. Third, in the clustering analysis using dendrogram, group A(Shangahi and Busan), group B(Ningbo and Nagoya), and group C(Incheon and Manila) show the common clustering ports during 3 or 2 years. The policy implication of this paper is that Korean port policy planner should introduce the game cross-efficiency method when measuring the individual port efficiency. Also port authority should consider the merits of the clustering ports for improving the port management and operations.

Ecological Characteristics of Vascular Plants by Habitat Types of Dry Field in Jeolla-do, Korea (전라도 밭경작지의 서식처 유형별 식물상 특성)

  • Cho, Kwang-Jin;Kim, Myung-Hyun;Kim, Min-Kyeong;Na, Young-Eun;Oh, Young-Ju;Choe, Lak-Jung
    • Korean Journal of Environmental Agriculture
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    • v.33 no.2
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    • pp.86-102
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    • 2014
  • BACKGROUND: According to the types of human interference, there are various plants that have strong vitality and ability to breed in the dry field. Recently, climate change alters the geographical distribution and phenology of the plant species. So, we need to understand present occurrence pattern and ecological characteristics of these plants. METHODS AND RESULTS: The plant species data were obtained from 8 regions in Jeolla-do. Flora investigation was done from May 2013 to September 2013. Habitat type of dry field in Jeolla-do was classified into 3 types (inside of dry field: IDF, embankment around the end of a dry field: EDF, levee slope of dry field: LS). The vascular plants of study area were listed 296 taxa which contain 68 families, 203 genera, 244 species, 43 varieties and 9 forms. The vascular plants of three different habitat types were IDF 174 taxa, EDF 249 taxa and LS 136 taxa. The occurrence rate of Therophyte was arranged by the order of IDF(67.6%), EDF(51.9%), LS(54.3%). Naturalized rate was analysed as IDF 27.9%, EDF 21.0%, LS 18.6%. Urbanization index was analysed as IDF 11.8%, EDF 13.7%, LS 10.0%. CONCLUSION: With these results, we found that three habitat types were ecological difference affected by the human impacts. Also, we found environmental indicators through the ecological characteristics of flora for the type of habitat of dry field. These indicators will help assess the agriculture environmental variability and the floral change according to the climate change in dry field.

Effect of Aqueous Extracts from Rubus coreanus Miquel and Angelica gigas Nakai on Anti-tumor and Anti-stress activities in mice (복분자와 당귀 열수추출물의 마우스를 이용한 항암 및 항스트레스 효과)

  • Kim, Jung-Hwa;Kim, Cheol-Hee;Kim, Hyou-Sung;Kwon, Min-Chul;Song, Young-Kyu;Seong, Nak-Sul;Lee, Seung-Eun;Yi, Jae-Seon;Kwon, Oh-Woung;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.206-211
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    • 2006
  • This study was performed to examine antitumor activities of Rubus coreanus Miquel and Angelica gigas Nakai extracts against sarcoma-180 and anti-stress activities in ICR mice. The variation of body weights of the 20 days of Rubus coreanus extracts-administrated mice group was very low. The survival rate (T/C %) of Rubus coreanus extract administrated group was 161% after 50 days from the inoculation of sarcoma-180 and the increment of their body weights was suppressed. Anti-stress effect of the extracts of R. coreanus and A. gigas were estimated by maeasuring blood chemical value and internal organs weight in ICR mice. The extracts of R. coreanus reduced the cholesterol and glucose to the normal level in the all stress animal models. The extracts of R. coreanus reduced the hypertrophy of the internal organs such as adrenal, spleen and liver to the regular level.

Concurrent Cisplatin-Radiation Therapy in Locally Advanced Head & Neck Cancers - Preliminary Report - (국소진행된 두경부종양의 Cisplatin-방사선 동시병용치료 - 예비적 임상결과보고 -)

  • Kim In Ah;Choi Ihl Bhong;Cho Seung Ho;Hong Young Seon;Choi Byung Ok;Kang Young Nam
    • Radiation Oncology Journal
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    • v.19 no.3
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    • pp.205-210
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    • 2001
  • Purpose : This study tried to evaluate the effectiveness of combined treatment using radiation therapy and concurrent cisplatin as a radiosensitizer in the management of locally advanced head and neck cancer. Materials and methods : From January 1995 to August 1998, 29 evaluable patients with locally advanced head & neck cancels (AJCC stage $II\~IV$) were received curative radiation therapy $(total\;70\~75.6\;Gy/35\~42\;fractions,\;1.8\~2\;Gy/fraction)$ and concurrent cisplatin chemotherapy ($100\;mg/m^2$, D1, D22, D43). The neck dissections were peformed for residual lymphadenopathy. Follow-up ranged from 5 to 55 months (median 24 months). Results : Twenty-one $(72.4\%)$ patients achieved clinical complete responses. The partial response and minimal response rates were $17.2\%\;and\;10.4\%$, respectively. Locoregional failure rate was $27.6\%$, and included 6 patients with local failures, 4 patients with regional failures, and 2 patients with combined local and regional failures. Four of 29 patients $(13.8\%)$ developed distant metastasis. The disease free survival rate at 3 years was $60\%$. Nasopharyngeal primary tumors or complete responders showed significantly higher disease free survival rate. The grade 3 mucositis and nausea/vomiting was noted in $34.5\%$, respectively. Major prolongation of radiation therapy duration was inevitable in three patients. Twenty-one patients $(72.4\%)$ completed 3 courses of cisplatin and 5 patients received 2 courses of cisplatin. Three patients received only one course of cisplatin due to nephrotoxicity and neurotoxicity, and then changed to 5-FU regimen. Conclusions : Concurrent cisplatin-radiation therapy in locally advanced head and neck cancer showed high response rate, reasonable locoregional control, and survival rate. As expected, acute toxicities were increased, but compliance to treatment was acceptable. Assessment of the effect of the combination in this setting requires further accrual and follow-up.

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Effect of Exogenous Application of Salicylic Acid or Nitric Oxide on Chilling Tolerance and Disease Resistant in Pepper Seedlings (외생 살리실산과 일산화질소 처리가 고추묘의 저온 내성 및 병 저항성에 미치는 영향)

  • Park, Song-Yi;Kim, Heung-Tae;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.329-336
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    • 2014
  • As an abiotic stress, chilling stress is one of the major factors limiting plant growth and increasing susceptibility to pathogens. Therefore, enhancing stress tolerance in plants is an important strategy for their survival under unfavorable environmental conditions. The objective of this study was to determine the effects of the exogenous application of salicylic acid (SA) or nitric oxide (NO) on chilling tolerance in pepper seedlings. Pepper (Capsicum annuum L. 'kidaemanbal') seedlings were grown under normal growing conditions ($20/25^{\circ}C$, 15 hours photoperiod, $145{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, fluorescence lamps) for 23 days after transplanting. The solution (3 mL) of 1 mM SA and 0.3 mM NO with surfactant triton 0.1% were sprayed two times a week, respectively. Right after the completion of chemical application, seedlings were subjected to chilling condition at $4^{\circ}C$ for 6 hours under dark condition and then the seedlings were recovered at the normal growing conditions for 2 days. In order to assess plant tolerance against chilling stress, growth characteristics, chlorophyll fluorescence (Fv/Fm), and membrane permeability were determined after chilling stress imposition. Total phenolic concentration and antioxidant capacity were measured during the whole experimental period. Disease incidence for pepper bacterial spot and wilt was also analyzed. Pepper seedlings treated with SA or NO were maintained similar dry mass ratio, while the value in control increased caused by chilling stress suggesting relatively more water loss in control plants. Electrolyte leakage of pepper seedlings treated with SA or NO was lower than that of control 2 days after chilling treatment. Fv/Fm rapidly decreased after chilling stress in control while the value of SA or NO was maintained about 0.8. SA increased higher total phenolic concentration and antioxidant capacity than NO and control during chemical treatment. In addition, increase in total phenolic concentration was observed after chilling stress in control and NO treatment. SA had an effect on the reduction of bacterial wilt in pepper seedlings. The results from this study revealed that pre-treatment with SA or NO using foliar spray was effective in chilling tolerance and the reduction of disease incidence in pepper seedlings.

On Method for LBS Multi-media Services using GML 3.0 (GML 3.0을 이용한 LBS 멀티미디어 서비스에 관한 연구)

  • Jung, Kee-Joong;Lee, Jun-Woo;Kim, Nam-Gyun;Hong, Seong-Hak;Choi, Beyung-Nam
    • 한국공간정보시스템학회:학술대회논문집
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    • 2004.12a
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    • pp.169-181
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    • 2004
  • SK Telecom has already constructed GIMS system as the base common framework of LBS/GIS service system based on OGC(OpenGIS Consortium)'s international standard for the first mobile vector map service in 2002, But as service content appears more complex, renovation has been needed to satisfy multi-purpose, multi-function and maximum efficiency as requirements have been increased. This research is for preparation ion of GML3-based platform to upgrade service from GML2 based GIMS system. And with this, it will be possible for variety of application services to provide location and geographic data easily and freely. In GML 3.0, it has been selected animation, event handling, resource for style mapping, topology specification for 3D and telematics services for mobile LBS multimedia service. And the schema and transfer protocol has been developed and organized to optimize data transfer to MS(Mobile Stat ion) Upgrade to GML 3.0-based GIMS system has provided innovative framework in the view of not only construction but also service which has been implemented and applied to previous research and system. Also GIMS channel interface has been implemented to simplify access to GIMS system, and service component of GIMS internals, WFS and WMS, has gotten enhanded and expanded function.

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