• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1544-1549
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• 2024

This study presents a fluorescent mechanism for two-step amplification by combining two widely used techniques, exponential amplification reaction (EXPAR) and catalytic hairpin assembly (CHA). Pseudomonas aeruginosa (P. aeruginosa) engaged in competition with the complementary DNA in order to attach to the aptamer that had been fixed on the magnetic beads. The unbound complementary strand in the liquid above was utilized as a trigger sequence to initiate the protective-EXPAR (p-EXPAR) process, resulting in the generation of a substantial quantity of short single-stranded DNA (ssDNA). The amplified ssDNA can initiate the second CHA amplification process, resulting in the generation of many double-stranded DNA (dsDNA) products. The CHA reaction was initiated by the target/trigger DNA, resulting in the release of G-quadruplex sequences. These sequences have the ability to bond with the fluorescent amyloid dye thioflavin T (ThT), generating fluorescence signals. The method employed in this study demonstrated a detection limit of 16 CFU/ml and exhibited a strong linear correlation within the concentration range of 50 CFU/ml to 10⁵ CFU/ml. This method of signal amplification has been effectively utilized to create a fluorescent sensing platform without the need for labels, enabling the detection of P. aeruginosa with high sensitivity.

• Journal of Digital Contents Society
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• v.19 no.3
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• pp.561-567
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• 2018

In this paper, we apply this method to the entire process of smart disaster safety management based on the $4^{th}$ industrial revolution to minimize human, social, economic and environment damage from accidents and disasters, prevention evaluation and disaster information collection analysis and real-time detection of field situation. Prevention of $5^{th}$ generation communication system by analysis, contrast by education and training using virtual reality and augmented reality disaster safety management decision support system intelligent robot for recovery, disaster, discovery, reconnaissance relief, and scale analysis of damages were proposed.

• Fire Science and Engineering
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• v.31 no.6
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• pp.112-117
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• 2017

This research discusses the correlation about fire mechanism based on real fire cases. Electric power condenser failure mechanism is classified into 7 steps and fire mechanism is classified into 12 steps. In the 5th step, the procedure of operating a protection channel of a protection relay was identical in the case of the failure and fire. As the fire occurrence mechanism was applied from the 6th step, internal pressure was increased because of gas generation produced by internal combustion phenomenon and arc. This caused explosion in 10st step of fire occurrence mechanism. In 11th step, the flame such as arc gushed out with insulating oil which caused fire and leaded to second accident. This kind of step correlation could play an important part to examine fire.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.1
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• pp.21-23
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• 2011

In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.

• Journal of Sericultural and Entomological Science
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• v.31 no.1
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• pp.20-24
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• 1989

The purpose of this experiment was to obtain the basic information on heterosis and inbreeding depression of quantitative characters in the silkworm by diallel crosses of seven varieties differing widely in each character. Period of 5th instar cocoon weight, cocoon layer weight, cocoon layer ratio, boiling off ratio, raw silk percent, bave length, bave weight, bave size, weight of first molting larva and fourth molting larva were investigated. The results obtained were summarized as follows : The degrees of positive heterosis of cocoon weight and cocoon layer weight were higher. Period of 5th instar and boiling off ratio were negative heterosis. In F1 generation, cocoon weight and bave weight showed positive heterobeltiosis, while fifth instar-period, cocoon layer ratio and boiling off ratio showed negative heterobeltiosis in the F1 and F2 generation. Inbreeding depression was observed in cocoon layer ratio and boiling off ratio.

• Education of Primary School Mathematics
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• v.22 no.1
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• pp.1-12
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• 2019

This study examined the attention and attention shift of general students and mathematically gifted students about pattern by the types of mathematical patterns. For this purpose, we analyzed eye movements during the problem solving process of 5th general and mathematically gifted students using eye tracker. The results were as follows: first, there was no significant difference in attentional style between the two groups. Second, there was no significant difference in attention according to the generation method between the two groups. The diversion was more frequent in the incremental strain generation method in both groups. Third, general students focused more on the comparison between non-contiguous terms in both attributes. Unlike general students, mathematically gifted students showed more diversion from geometric attributes. In order to effectively guide the various types of mathematical patterns, we must consider the distinction between attention and attention shift between the two groups.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4776-4797
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• 2022

In the one-dimensional thermal-hydraulic (TH) codes, a subcooled boiling model to predict the void fraction profiles in a vertical channel consists of wall heat flux partitioning, the vapor condensation rate, the bubbly-to-slug flow transition criterion, and drift-flux models. Model performance has been investigated in detail, and necessary refinements have been incorporated into the Safety and Performance Analysis Code (SPACE) developed by the Korean nuclear industry for the safety analysis of pressurized water reactors (PWRs). The necessary refinements to models related to pumping factor, net vapor generation (NVG), vapor condensation, and drift-flux velocity were investigated in this study. In particular, a new NVG empirical correlation was also developed using artificial neural network (ANN) techniques. Simulations of a series of subcooled flow boiling experiments at pressures ranging from 1 to 149.9 bar were performed with the refined SPACE code, and reasonable agreement with the experimental data for the void fraction in the vertical channel was obtained. From the root-mean-square (RMS) error analysis for the predicted void fraction in the subcooled boiling region, the results with the refined SPACE code produce the best predictions for the entire pressure range compared to those using the original SPACE and RELAP5 codes.

• Journal of Digital Convergence
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• v.18 no.12
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• pp.267-278
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• 2020

ESS is an essential requirement for resolving power shortages and power demand management and promoting renewable energy at a time when the energy paradigm changes. In this paper, we propose a cost-effective ESS Peak-Shaving operation plan through load and solar power generation forecast. For the ESS operation plan, electric load and solar power generation were predicted through RMS, which is a statistical measure, and a target load reduction guideline for one hour was set through the predicted electric load and solar power generation amount. The load and solar power generation amount from May 6th to 10th, 2019 was predicted by simulation of load and photovoltaic power generation using real data of the target customer for one year, and an hourly guideline was set. The average error rate for predicting load was 7.12%, and the average error rate for predicting solar power generation amount was 10.57%. Through the ESS operation plan, it was confirmed that the hourly guide-line suggested in this paper contributed to the peak-shaving maximization of customers.Through the results of this paper, it is expected that future energy problems can be reduced by minimizing environmental problems caused by fossil energy in connection with solar power and utilizing new and renewable energy to the maximum.

• Korean Journal of Veterinary Research
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• v.60 no.4
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• pp.203-207
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• 2020

This study presents neuronal migration pattern of dopamine (DA) neurons generated in separate regions occupying the ventral mesencephalic territory. A single pulse 5-bromodeoxyuridine (BrdU) was administered at embryonic day (E)10-E15. Distribution of tyrosine hydroxylase (TH) positive cells was determined at E13-postnatal day 0 (P0) by immunohistochemistry. BrdU positive cells labeled at E10 were spread out uniformly in the mesencephalon from E13 to E15, migrating through dorsal and ventral routes at E17 and P0. TH expression labeled at E10 was observed at E13 in the ventromedial region and clearly formed in the ventral tegmental area (VTA) at E15. At E17, TH expression in the substantia nigra (SN) was observed in the ventrolateral region, spreading more outward of the mesencephalon at P0. Generation of TH-positive cells labeled at E13 was also observed in VTA and SN of the mesencephalon at E17 and P0. The expression of these cells labeled after E15 was markedly decreased. These results demonstrated that an almost complete primary structure of DA neuron was formed at the early embryonic stage in the ventral mesencephalon, showing the most active neuronal migration was occurred at E13-E17.

• Korean journal of applied entomology
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• v.24 no.3 s.64
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• pp.151-156
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• 1985

Apple leaf miner(ALM), Phyllonorycter ringoniella(Matsumura), occurs $4{\sim}5$ generations a year in Suweon; adult emergence peaks being in mid April, early June, early July, mid August, and mid September with the highest one in the 4th generation. Numbers of days required to complete the development(egg to adult emergence) were different with oviposited dates; 43days for late May, $32{\sim}37$ days for June to July, and 39days for early August. ALM larvae oviposited after late August did not emerge and went into diapause. Sites of the infested leaf on the shoot seemed to be somewhat different with the ALM generations; the preferred leaf sites being the 1st-6th leaf for the 1st, the 4th-9th for the 2nd, the 4th-18th for the 3rd or 4th, and the terminal leaves of the first growth shoot or the leaves of the secondary shoot for the 5th generation. Parasites of three families emerged from the mines of ALM in Suweon. The encyrtid (Holcothorax testaceipes Ratzelburg) occurs 4 generations and the eulophids 5 generations a year. A small number of the braconid(Apanteles sp.) occcrred only in September.