• Journal of Korean Society on Water Environment
• /
• v.26 no.4
• /
• pp.622-628
• /
• 2010

In this study, Solid-phase microextraction (SPME) with GC/FID was studied as a possible alternative to liquid-liquid extraction for the analysis of BTEX and MTBE. Experimental parameters affecting the SPME process (such as kind of fibers, adsorption time, desorption time, volume ratio of sample to headspace, salt addition, and magnetic stirring) were optimized. Experimental parameters such as CAR/PDMS, adsorption time of 20 min, desorption time of 5 min at $250^{\circ}C$, headspace volume of 50 mL, sodium chloride (NaCl) concentration of 25% combined with magnetic stirring were selected in optimal experimental conditions for analysis of BTEX and MTBE. The general affinity of analytes to CAR/PDMS fiber was high in the order p-Xylene>Toluene>Ethylbenzene>MTBE>Benzene. The linearity of $R^2$ for BTEX and MTBE was from 0.970 to 0.999 when analyte concentration ranges from $30{\mu}g/L$ to $500{\mu}g/L$, respectively. The relative standard deviation (% RSD) were from 2.5% to 3.2% for concentration of $100{\mu}g/L$ (n=5), respectively. Finally, the limited of detection (LOD) observed in our study for BTEX and MTBE were from $7.5{\mu}g/L$ to $15{\mu}g/L$, respectively.

• Journal of Biosystems Engineering
• /
• v.9 no.1
• /
• pp.11-21
• /
• 1984

This study was intended to analyze the dynamic force system which induced the shattering of paddy grains. A model to predict the shattering of paddy grains was developed, and physical quantities, such as mass distribution and rigidity of rice plant, needed for evaluating the minimum shattering forces were also measured. Under the assumption that rice plant right before harvesting is a vibratory system, the mathematical model of the vibratory system was developed and solved with the varied conditions of forcing functions. The results of the study were summarized as follows: 1. The shattering of grain occurred at the abscission layer of grain by the bending moments resulted from the impact force due to the collision of panicles of rice plant. 2. The vibratory model developed for milyang 23 rice variety was analyzed to give the natural frequencies of 7-9 Hz, which were closely related with the excitation frequencies of 4-10 Hz caused by various machine parts besides engine. Thus, avoiding the resonance should be taken into consideration in the design of the harvesting machinery. 3. It was analyzed to predict the lowest frequency that could develop the shattering when the excitation force was applied to the lower end of stem. The lowest frequency for the Milyang 23 rice variety ranged from 8.33 Hz to 11.66 Hz as the amplitude varied from 1 cm to 2.5 cm. 4. The degree of shattering depended upon the magnitude of the impact force and its application point. For Milyang 23 rice variety, the minimum impact force developing the shattering was $5g_f$ when it was applied at 1 cm above the lower end of stern and $1g_f$ when applied at 5 cm above the lower end of stem. 5. The minimum colliding velocity of the panicle, when it was on the ground that would just develop the shattering, was given as follows, $$V=\sqrt{\frac{K_t}{m_g}{\cdot}{{\phi}^2}}$$ where V : The colliding velocity of the panicle against ground to cause the shatteering of rice grain. (cm/sec) $K_t$ : The minimum spring constant for bending at the abscission layer of grain. (dyne-cm/rad) ${\phi}$ : The minimum shattering angle of grain (rad) $m_g$ : The maximum mass of grain. (g).

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.27 no.8
• /
• pp.725-734
• /
• 2016

Recently, novel candidate waveform techniques for spectral efficiency improvement was proposed in order to satisfy key performance indicators(KPIs) of 5th generation(5G) mobile communication. Multi-carrier based universal filtered multi-carrier(UFMC) and filter bank multi-carrier(FBMC) are very famous as 5G candidate waveform techniques. Also, weighted orthogonal frequency division multiplexing (W-OFDM) that has low-complexity is receiving the spotlight slowly. In this paper, firstly, we describe a basic OFDM system. And then, we also describe UFMC, FBMC, and W-OFDM system. Next, we evaluate and analyze spectrum and BER performance of these systems under the nonlinear high power amplifier(HPA) environment. As simulation results, spectrum characteristic and BER performance of UFMC, FBMC, and W-OFDM are similar to each other. Therefore, under the nonlinear HPA environment, W-OFDM system is more advantageous because W-OFDM system uses a simple time-domain windowing technique and has similar characteristics to the others.

• KSBB Journal
• /
• v.5 no.3
• /
• pp.269-277
• /
• 1990

Methylobacillus sp. SK1, an obligate methylotroph, was cultivated in a fed-batch culture using DO as a methanol feeding indicator with a micro computer-aided control system. While 2.07g/l of cell density was obtained after 13 hr in the batch culture (initial methanol concentration: 1.0%(v/v)),45.3g/l of cell density was obtained after 17 hr by feeding methanol and metal ions in the fed-batch culture with oxygen supply. The high-density biomass was obtained in short cultuivation time by fed-batch culture with feedback control, and consequently the biomass productivity was significantly increased. It was mainly due to extension of logarithmic growth period by methanol feeding without methanol inhibition and intensive aeration without DO limitation with microcomputer-aided control system.

• Proceedings of the Korean Biophysical Society Conference
• /
• 2003.06a
• /
• pp.59-59
• /
• 2003

Serotonin (5-HT; 5-hydroxytryptamine) exerts multiple effects on central nervous system as well as behaviors such as mood and appetite. The signaling of serotonin is mediated by 7 families of serotonin receptors, designated 5-HT$_1$ to 5-HT$_{7}$. Six families of this receptor are G-protein coupled 7TM receptors, and the third intracellular loop of these receptors is proposed to interact with specific types of G-proteins. To investigate the specific interaction between the third intracellular loop of 5-HT$_{6}$ with G$\square$s, we have constructed a chimera protein that represent the third intracellular loop of 5-HT$_{6}$ within a leucine zipper motifs, In addition an alpha subunit of human G-protein that interact with 5-HT$_{6}$ was cloned into a bacterial expression vector. The two proteins were expressed in E. coli and purified in homogeneity. The interaction of the prepared proteins was examined by ELISA assay. The affinity between the two proteins and effect of insertion mutations were discussed.ussed.d.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.50 no.12
• /
• pp.270-276
• /
• 2013

We have been unwittingly exposed to magnetic field. Biological effects due to electromagnetic field exposure has been studied over the past several decades. There has been epidemiological studies and laboratory studies. In the case of laboratory studies, the effect has been observed through the cell stimulation of an artificial magnetic field. The used frequency and waveform are various in the cell experiment, but the intensity of the magnetic field is usually around 10G. Intensity of Earth's magnetic field is about 300-400mG. Although this intensity is not much higher than artificial magnetic field as 3-4% amplitude, we can't ignore the effect because every living thing on earth has already adapted for long time ago. In this study, we have designed and implemented the 3-axis magnetic field generation system in oder to remove the static magnetic field in the real-time and kept its intensity less than ${\pm}5mG({\pm}0.5uT)$ using 3-axis helmholtz coil.

• Journal of Ginseng Research
• /
• v.38 no.1
• /
• pp.73-77
• /
• 2014

Field and nutrient cultures of American ginseng (Panax quinquefolius L.) were used to establish foliar symptoms related to boron (B) concentration in leaves and soils, and to evaluate radish as a time-saving model system for B nutrition. Application of excess B, 8 kg/ha versus the recommended 1.5 kg/ha, to field plantings of 2-, 3-, and 4-yr-old American ginseng plants just prior to crop emergence caused, within 4 wk after crop emergence, leaf symptoms of chlorosis followed by necrosis starting at the tips and progressing along the margins. The B concentration in leaves of 2-4-yr-old plants receiving 1.5 kg/ha Bwas $30{\mu}g/g$ dry mass compared to $460{\mu}g/g$ dry mass where 8 kg/ha B was applied. Similarly, B concentration in soils receiving the lower B concentration was 1.8 mg/g dry mass and $2.2-2.8{\mu}g/g$ dry mass where the higher B concentration was applied. Application of 8 kg/ha B reduced the dry yield of 3rd-yr roots by 20% from 2745 kg/ha to 2196 kg/ha and 4th-yr roots by 26% from 4130 kg/ha to 3071 kg/ha. Ginseng seedlings and radish were grown under greenhouse conditions in nutrient culture with four B concentrations ranging from 0 mg/L to 10 mg/L. At 5 mg/L and 10 mg/L ginseng and radish developed typical leaf B toxicity symptoms similar to those described above for field-grown plants. Increasing B in the nutrient solution from 0.5 mg/L to 10 mg/L decreased, in a linear fashion, the root and leaf dry mass of ginseng, but not radish. Given the many similarities of ginseng and radish to B utilization, radish might be used as a timesaving model system for the study of B, and other micronutrients, in the slow-growing perennial ginseng.

• Electronics and Telecommunications Trends
• /
• v.36 no.4
• /
• pp.61-71
• /
• 2021

Navigation satellite systems (GPS, GLONASS etc.) provide three main services, i.e., positioning for location based services, navigation for multi-modal transportation services, and timing for communication and critical infrastructure services. They were started as military systems but were extended to civil service. Navigation satellite navigation system began with GPS in the USA and GLONASS in Russia at nearly the same time. Indian NavIC and Chines BDS announced their FOCs in 2016 and 2020, respectively and European Galileo and Japanese QZSS are catching up others. In these days, Navigation Satellite System, Positioning, Navigation, and Timing services are part of our daily life very closely. They are required for autonomous driving car, Unmanned vehicles like UAV, UGV, and UMV, 5G/6G telecommunications, world financial system, power system, survey, agriculture, and so on. The services among navigation satellite systems are very competitive and also cooperative one another. This article describes the status of these systems and evolution in the technical and service senses, which may be helpful for planning korea positioning system(KPS).

• KSBB Journal
• /
• v.16 no.5
• /
• pp.511-515
• /
• 2001

In this paper, we development and operated a two-stage continuous stirred tank reactor (CSTR)/trickling biofilter(TBF)system for the long-term continuous treatment of trichloroethylene (TCE) using Burkholderia cepacia G4. In this reactor system. CDTR with cell recycle from TBF was coupled to the TBF for the reactivation of the cells deactivated during TCE degradation. The critical elimination capacity was determined to be 25.3 mg TCE/L day and the reactor has been stably operated for more than 1 months, which clearly represented that CSTR/TBF system can be used for long-term treatment of TCE.

• Development and Reproduction
• /
• v.14 no.1
• /
• pp.13-19
• /
• 2010

We developed a novel dicistronic system for the expression of target cDNA sequences in the milk of transgenic animals using goat beta-casein/hGH fusion construct, pGbc5.5hGH (Lee, 2006) and internal ribosome entry site (IRES) sequences of encephalomyocarditis virus (EMCV). Granulocyte colony-stimulating factor (hG-CSF) cDNA was linked to 3' untranslated region of hGH gene in the pGbc5.5hGH via EMCV IRES sequences. Transgenic mice were generated by microinjection and transgene expression was examined in the milk and mammary gland of transgenic mice at 10 days of lactation. Northern blot analysis showed that hGH gene and hG-CSF cDNA were transcribed as a single dicistronic mRNA. The hG-CSF and hGH proteins were independently translated from the dicistronic mRNA and secreted into the milk of transgenic mice. The highest concentration of hG-CSF and hGH in the milk of transgenic mice were $237{\mu}g/m{\ell}$ and $8,990{\mu}g/m{\ell}$, respectively. In contrast, another hG-CSF expression cassette, in which hG-CSF genomic sequences were inserted into a commercial milk-specific expression vector (pBC1), generated a lower level ($91{\mu}g/m{\ell}$) of hG-CSF expression in the milk of transgenic mice. These results demonstrated that the novel pGbc5.5hGH-based dicistronic construct could be useful for an efficient cDNA expression in the milk of transgenic animals.