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Optimization of Indole-3-Acetic production by phosphate solubilization bacteria isolated from waste mushroom bed of Agaricus bisporus

  • Walpola, Buddhi Charana;Noh, Jae-Geun;Kim, Chan Kyem;Kyung, Ki-Cheon;Kong, Won-Sik;Yoon, Min-Ho
    • Journal of Mushroom
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    • v.11 no.2
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    • pp.53-62
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    • 2013
  • A total of 35 phosphate solubilizing bacterial strains were isolated from waste mushroom bed of Agaricus bisporus in Buyeo-Gun, Chungnam and screened for the production of indole acetic acid (IAA). The best IAA producing strain was identified as Pantoea rodasii using 16S rRNA analysis. In addition to the IAA production, this strain could act as an efficient phosphate solubilizer (1100 ${\mu}g$ $ml^{-1}$ after 5 days of incubation) also. The selected strain was cultured under different conditions in order to assess the optimum conditions for maximum IAA production. The nutrient broth (NB) medium was recorded as the best medium, where the maximum IAA production (229 ${\mu}g$ $ml^{-1}$) was recorded at the start of stationary phase (12 hours after inoculation) of the bacteria growth. The performance of the strain was found to be maximum at the temperature of $30^{\circ}C$ followed by $25^{\circ}C$. IAA production was found to be increased with increasing tryptophan concentration (from 0.1 to 0.6%), however beyond this limit, a slight reduction in IAA production was observed. The strains' ability to produce IAA was further confirmed by extraction of crude IAA and subsequent TLC analysis. A specific spot from the extracted IAA preparation was found corresponding with the standard spot of IAA with same $R_f$ value. The results of HPLC analysis conducted in identifying and quantifying the IAA production more precisely, are in agreement with the results of the assessment done with colorimetric method. As revealed by the results of the pot experiment, the isolated strain could significantly enhance the growth (as measured by shoot and root growth) of mung bean plants compared to that of non-inoculated plants. Therefore it can be concluded that the present strain, Pantoea rodasii has great potential to be used as bio-inoculants.

Changes in Endogenous Gibberellin Contents during Bulb Development Period in the Cold-type Cultivar of Garlic (Allium sativum L.) of Korea (한지형 마늘의 인경 발육 과정에서 내생 지베렐린류의 함량변화)

  • Sohn, Eun-Young;Kim, Yoon-Ha;Kim, Byung-Su;Seo, Dong-Hwan;Lee, Hyun-Suk;Lee, In-Jung
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.750-756
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    • 2010
  • This study was performed to investigate the role of phytohormones in the bulbing of garlic in order to assess the yield and quality. The effect on endogenous plant hormones such as gibberellin (GA) content was also examined during growth stage i.e. clove differentiation to bulbing in garlic. More than 18 gibberellins in garlic were identified with extensive gas chromatograph-mass spectrometry-selected ion monitoring (GC-MS-SIM) quantitative analysis. The results showed that GAs were biosynthesized by both non C-13 hydroxylation pathway (NCH) and early C-13 hydroxylation pathway (ECH) in garlic plant. It was also revealed that NCH pathway leading to synthesis of bioactive $GA_4$ was the more prominent GA biosynthesis pathway than ECH pathway in which bioactive $GA_1$ was synthesized. Total GAs level was gradually increased from clove differentiation to bulbing and later decreased, which portrays the active role of GA in differentiation. The biosynthesis ratio of bioactive $GA_4$ and $GA_1$ concentration was similar to that of total GAs content, which was closely related with bulb development in garlic.

Predictors of Protective Factors for Depression in Adolescent using Decision Making Tree Analysis (의사결정나무분석을 이용한 청소년 우울의 보호요인 예측모형)

  • Kim, Bo-Young
    • The Journal of the Korea Contents Association
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    • v.15 no.5
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    • pp.375-385
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    • 2015
  • The study is to develop specific strategies to prevent adolescents' depression, early detection and intervention services. This study was a descriptive research study with the purpose of predictors of protective factors for depression in adolescent using decision making tree analysis. The subjects for the study were 485 student in G city. This study collected data between September 23, 2013 and September 26, 2013 and analyzed them with frequency analysis, percentage, the mean and standard deviation, ${\chi}^2$-test, t-test, and a decision-making tree by using SPSS 20.0 program. From the data analysis, the predictive model for protective factors related to depression in adolescent with 4 pathways, 12 nodes. The common predicting variables of depression in adolescent were characteristics, family cohesion, parent adolescent communication, peer communication. The specialty of training data and test data was 76.0% and 65.4%. The sensitivity of training data was 78.2% and 63.7%. As for the classification accuracy, training data and test data explained 70.1% and 69.7%. Parent adolescent communication and peer communication to decrease depression of Korean middle and high school students are necessary. This study should contribute as baseline data for intervention strategies and planning ability of depression prevention in adolescents.

Fall Detection for Mobile Phone based on Movement Pattern (스마트 폰을 사용한 움직임 패턴 기반 넘어짐 감지)

  • Vo, Viet;Hoang, Thang Minh;Lee, Chang-Moo;Choi, Deok-Jai
    • Journal of Internet Computing and Services
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    • v.13 no.4
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    • pp.23-31
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    • 2012
  • Nowadays, recognizing human activities is an important subject; it is exploited widely and applied to many fields in real-life, especially in health care and context aware application. Research achievements are mainly focused on activities of daily living which are useful for suggesting advises to health care applications. Falling event is one of the biggest risks to the health and well-being of the elderly especially in independent living because falling accidents may be caused from heart attack. Recognizing this activity still remains in difficult research area. Many systems equipped wearable sensors have been proposed but they are not useful if users forget to wear the clothes or lack ability to adapt themselves to mobile systems without specific wearable sensors. In this paper, we develop a novel method based on analyzing the change of acceleration, orientation when the fall occurs and measure their similarity to featured fall patterns. In this study, we recruit five volunteers in our experiment including various fall categories. The results are effective for recognizing fall activity. Our system is implemented on G1 smart phone which are already plugged accelerometer and orientation sensors. The popular phone is used to get data from accelerometer and results showthe feasibility of our method and significant contribution to fall detection.

Effects of Reclaimed Wastewater and Waste Nutrient Solution Irrigation on Seedling Growth of Chinese Cabbage (하수처리수 및 폐양액의 재이용이 배추 유묘 생장에 미치는 영향)

  • Hong, Ki-Chan;Choi, Bong-Su;Lim, Kyoung-Jae;Won, Jae-Hee;Jeon, Shin-Jae;Hur, Seung-Oh;Ha, Sang-Keun;Kim, Nam-Won;Yang, Jae-E;Ok, Yong-Sik
    • Korean Journal of Environmental Agriculture
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    • v.28 no.2
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    • pp.171-178
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    • 2009
  • Water shortages are expected to be a major impact of climate change. This study examined the growth of Chinese cabbage seedling using reclaimed wastewater and waste nutrient solution as alternative irrigation resources. Generally, the concentration of nutrients, such as $K^+$, $NH_4^+$, $Mg^{2+}$, $Ca^{2+}$, $Cl^-$,$NO_3^-$, $PO_4^-$ and $SO_4^{2-}$, in waste nutrient solution was higher than that in wastewater. However, Chinese cabbage seedling irrigated with wastewater was supplied a higher concentration of $Na^+$ and $Cl^-$ than waste nutrient solution. The growth of Chinese cabbage seedling irrigated with waste nutrient solution was similar or higher than those irrigated with groundwater as control, while the growth of those irrigated with wastewater was similar to those irrigated with groundwater. The total nitrogen uptake in Chinese cabbage seedling irrigated with groundwater, waste nutrient solution from organic and inorganic hydroponic cultures, and wastewater was 5.47, 10.02, 5.20, and 4.59 mg/plant, respectively. The nitrogen uptake of Chinese cabbage seedling irrigated with waste nutrient solution from organic hydroponic substrates in a 50% lower dose than recommended was 8.34 mg/plant, which is higher than that of the cabbage irrigated with groundwater. Overall, the results suggest that waste nutrient solution and wastewater can be used as alternate water resources, and can allow a reduction in the amount of fertilizer needed to raise Chinese cabbage seedling.

A Study on Mobile Antenna System Design with Tri-band Operation for Broadband Satellite Communications and DBS Reception (광대역 위성 통신/방송용 삼중 대역 이동형 안테나 시스템 설계에 관한 연구)

  • Eom Soon-Young;Jung Young-Bae;Son Seong-Ho;Yun Jae-Seung;Jeon Soon-Ick
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.5 s.108
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    • pp.461-475
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    • 2006
  • In this paper, it is described about the tri-band mobile antenna system design to provide broadband multimedia and direct broadcasting services using goo-stationary Koreasat 3, simultaneously operated in Ka/K/Ku band. The radiating part of the antenna system with a fan beam characteristic in the elevation plane is composed of the quasi-offset dual shaped reflector and the tri-band feeder. The tri-band feeder is also composed of the Ka/K dual band feeder with the protruding dielectric rod, the circular polarizer, the ortho-mode transducer and the circular-polarized Ku band feed array. Especially, the Ka/K dual band circular polarizer was realized firstly using the comb-type structure. For fast satellite-tracking on the movement, the Ku band feed array has the structure of the $2{\times}2$ active phased array which can make electrical beams. And, the circular-polarized characteristic in the feed array was improved by $90^{\circ}$ rotating arrangement of four radiating elements polarized circularly by a $90^{\circ}$ hybrid coupler, respectively. Four beam forming channels to make electrical beams at Ku band are divided into the main beam channel and the tracking beam channel in the output, and noise temperature characteristics of each channel were analyzed on the basis of the contributions of internal sub_units. From the fabricated antenna system, the output power at $P_{1dBc}$ of Ka_Tx channel was measured more than 34.1 dBm and the measured noise figures of K/Ku_Rx channels were less than 2.4 dB and 1.5 dB, respectively, over the operating band. The radiation patterns with co- and cross-polarization in the tri-band were measured using a near-field measurement in the anechoic chamber. Especially, Ku radiation patterns were measured after correcting each initial phase of active channels with partial radiation patterns obtained from the independent excitation of each channel. The antenna gains measured in Ka/K/Ku band of the antenna system were more than 39.6 dBi, 37.5 dBi, 29.6 dBi, respectively. And, the antenna system showed good system performances such as Ka_Tx EIRP more than 43.7 dBW and K/Ku_Rx G/T more than 13.2 dB/K and 7.12 dB/K, respectively.

Effect of Annual and Basal Dressing with Liquid Pig Manure on Growth and Quality of Rice in Double Cropping System of Rice-Malting Barley (벼.맥주보리 작부체계에서 돈분액비 연용이 벼 생육과 미질에 미치는 영향)

  • Lee, Seong-Tae;Seo, Dong-Cheol;Cho, Ju-Sik;Dahlgren, Randy A.;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.624-630
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    • 2010
  • In order to investigate the effects of continual pre-plant application of liquid pig manure (LPM) on growth and quality of rice in double cropping system of rice and malting barley, the liquid pig manure was applied after harvesting rice and malting barley for 3 years. Field experiment was designed with non-fertilizer, chemical fertilizer (CF) 100% recommended by soil testing, rice (LPM 50%+CF 50%)+malting barley (CF 100%), rice (LPM 50%+CF 50%)+malting barley (LPM 50%+CF 50%), rice (LPM 100%)+malting barley (CF 100%) and rice (LPM 100%)+malting barley (LPM 100%). The yield of rice was increased by 13% with increasing culm length and No. of panicle per hill as 482 kg $10a^{-1}$ in rice (LPM 100%)+malting barley (LPM 100%) plot compare with 427 kg $10a^{-1}$ in rice (CF 100%)+malting barley (CF 100%) plot. Whereas, lodging index was high in rice (LPM 100%)+malting barley (LPM 100%) plot by increasing of internodes length from 3rd to 4th and decreasing of breaking strength. When the quality of milled rice was compare with rice (CF 100%)+malting barley (CF 100%) plot, it was decreased by increasing of protein contents and decreasing of rate of perfect grain in rice (LPM 100%)+malting barley (LPM 100%) plot. The content of amylose was not significant by 19.4~22.0%. Toyo-taste value was not different in LPM 100% and CF 100% plot. Rice could be grown with only liquid pig manure 100%, but considering yield and quality of rice and lodging risk, basal fertilization by LPM 50% and top-dressing by CF 50% application was recommended for rice cultivation.

Trends of Study and Classification of Reference on Occupational Health Management in Korea after Liberation (해방 이후 우리나라 산업보건관리에 관한 문헌분류 및 연구동향)

  • Ha, Eun-Hee;Park, Hye-Sook;Kim, Young-Bok;Song, Hyun-Jong
    • Journal of Preventive Medicine and Public Health
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    • v.28 no.4 s.51
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    • pp.809-844
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    • 1995
  • The purposes of this study are to define the scope of occupational health management and to classify occupational management by review of related journals from 1945 to 1994 in Korea. The steps of this study were as follows: (1) Search of secondary reference; (2) Collection and review of primary reference; (3) Survey; and (4) Analysis and discussion. The results were as follows ; 1. Most of the respondents majored in occupational health(71.6%), and were working in university (68.3%), males and over the age 40. Seventy percent of the respondents agreed with the idea that classification of occupational health management is necessary, and 10% disagreed. 2. After integration of the idea of respondents, we reclassified the scope of occupational health management. It was defined 3 parts, that is , occupational health system, occupational health service and others (such as assessment, epidemiology, cost-effectiveness analysis and so on). 3. The number of journals on occupational health management was 510. It was sightly increased from 1986 and abruptly increased after 1991. The kinds of journals related to occupational health management were The Korean Journal of Occupational Medicine(18.2%), Several Kinds of Medical Colloge Journal(17.0%), The Korean Journal Occupational Health(15.1%), The Korean Journal of Preventive Medicine(15.1%) and others(34.6%). As for the contents, the number of journals on occupational health management systems was 33(6.5%) and occupational health services 477(93.5%). Of the journals on occupational health management systems, the number of journals on the occupational health resource system was 15(45.5%), occupational finance system 8(24.2%), occupational health management system 6(18.2%), occupational organization 3(9.1%) and occupational health delivery system 1 (3.0%). Of the journals on occupational health services, the number of journals on disease management was 269(57.2%), health management 116(24.7%), working environmental management 85(18.1%). As for the subjects, the number of journals on general workers was 185(71.1%), followed by women worker, white coiler workers and so on. 4. Respondents made occupational health service(such as health management, working environmental management and health education) the first priority of occupational health management. Tied for the second are quality analysis(such as education, training and job contents of occupational health manager) and occupational health systems(such as the recommendation of systems of occupational and general disease and occupational health organization). 5. Thirty seven respondents suggested 48 ideas about the future research of occupational health management. The results were as follows: (1) Study of occupational health service 40.5%; (2) Study of organization system 27.1%; (3) Study of occupational health system (e.g. information network) 8.3%; (4) Study of working condition 6.2%; and (5) Study of occupational health service analysis 4.2%.

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Various Cultural Factors Associated with Disease Development of Garlic White Rot Caused by Two Species of Sclerotium (마늘 흑색썩음균핵병 발생에 관여하는 여러가지 경종적 요인)

  • Kim, Yong-Ki;Kwon, Mi-Kyung;Shim, Hong-Sik;Kim, Tack-Soo;Yeh, Wan-Hae;Cho, Weon-Dae;Choi, In-Hu;Lee, Seong-Chan;Ko, Sug-Ju;Lee, Yong-Hwan;Lee, Chan-Jung
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.28-34
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    • 2005
  • This study was conducted to investigate the control possibility of garlic white rot causing severe yield losses of Allium species and cultivars using cultural practices such as optimal sowing date and burial depth, and lime application. Inoculum density in infested field soil was investigated at different soil depth, and that on the diseased plant debris was done. Inoculum density and recovery ratio of white rot pathogen of garlic was highly different between two species of Sclerotium cepivorum forming comparatively small sclerotia and Sclerotium sp. forming comparatively large ones. It was confirmed that S. cepivorum formed more sclerotia on bulbs of garlic than S. sp., and sclerotial recovery of S. cepivorum was higher than that of S. sp. Inoculum density of white rot pathogen in the infested field at garlic seeding period ranged from one to thirteen sclerotia per 30 g soil. Inoculum density of white rot pathogen decreased remarkably with increasing soil depth and above 95% of sclerotia were distributed within 5 cm of soil depth. Disease severity of white rot was higher on slightly planted garlics than deeply-planted ones. Garlic seed bulbs infected by white rot pathogens were confirmed to be one of main inoculum sources of white rot in the field and the disease incidences caused by garlic seed transmission showed big differences among garlic varieties. When nine garlic varieties harvested from infested plots were sown in the field, highly susceptible varieties, ‘Wando’, ‘Daeseo’, ‘Namdo’ and ‘Kodang’ showed high disease incidences, whereas other five varieties were not infected at all. It was confirmed that white rot occurred higher on early-sown garlics, before middle October, than on late-sown ones, after late October. Meanwhile, increasing application rate of lime ranged from 100 to 300 g reduced disease severity of white rot.

The Application of Operations Research to Librarianship : Some Research Directions (운영연구(OR)의 도서관응용 -그 몇가지 잠재적응용분야에 대하여-)

  • Choi Sung Jin
    • Journal of the Korean Society for Library and Information Science
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    • v.4
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    • pp.43-71
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    • 1975
  • Operations research has developed rapidly since its origins in World War II. Practitioners of O. R. have contributed to almost every aspect of government and business. More recently, a number of operations researchers have turned their attention to library and information systems, and the author believes that significant research has resulted. It is the purpose of this essay to introduce the library audience to some of these accomplishments, to present some of the author's hypotheses on the subject of library management to which he belives O. R. has great potential, and to suggest some future research directions. Some problem areas in librianship where O. R. may play a part have been discussed and are summarized below. (1) Library location. It is usually necessary to make balance between accessibility and cost In location problems. Many mathematical methods are available for identifying the optimal locations once the balance between these two criteria has been decided. The major difficulties lie in relating cost to size and in taking future change into account when discriminating possible solutions. (2) Planning new facilities. Standard approaches to using mathematical models for simple investment decisions are well established. If the problem is one of choosing the most economical way of achieving a certain objective, one may compare th althenatives by using one of the discounted cash flow techniques. In other situations it may be necessary to use of cost-benefit approach. (3) Allocating library resources. In order to allocate the resources to best advantage the librarian needs to know how the effectiveness of the services he offers depends on the way he puts his resources. The O. R. approach to the problems is to construct a model representing effectiveness as a mathematical function of levels of different inputs(e.g., numbers of people in different jobs, acquisitions of different types, physical resources). (4) Long term planning. Resource allocation problems are generally concerned with up to one and a half years ahead. The longer term certainly offers both greater freedom of action and greater uncertainty. Thus it is difficult to generalize about long term planning problems. In other fields, however, O. R. has made a significant contribution to long range planning and it is likely to have one to make in librarianship as well. (5) Public relations. It is generally accepted that actual and potential users are too ignorant both of the range of library services provided and of how to make use of them. How should services be brought to the attention of potential users? The answer seems to lie in obtaining empirical evidence by controlled experiments in which a group of libraries participated. (6) Acquisition policy. In comparing alternative policies for acquisition of materials one needs to know the implications of each service which depends on the stock. Second is the relative importance to be ascribed to each service for each class of user. By reducing the level of the first, formal models will allow the librarian to concentrate his attention upon the value judgements which will be necessary for the second. (7) Loan policy. The approach to choosing between loan policies is much the same as the previous approach. (8) Manpower planning. For large library systems one should consider constructing models which will permit the skills necessary in the future with predictions of the skills that will be available, so as to allow informed decisions. (9) Management information system for libraries. A great deal of data can be available in libraries as a by-product of all recording activities. It is particularly tempting when procedures are computerized to make summary statistics available as a management information system. The values of information to particular decisions that may have to be taken future is best assessed in terms of a model of the relevant problem. (10) Management gaming. One of the most common uses of a management game is as a means of developing staff's to take decisions. The value of such exercises depends upon the validity of the computerized model. If the model were sufficiently simple to take the form of a mathematical equation, decision-makers would probably able to learn adequately from a graph. More complex situations require simulation models. (11) Diagnostics tools. Libraries are sufficiently complex systems that it would be useful to have available simple means of telling whether performance could be regarded as satisfactory which, if it could not, would also provide pointers to what was wrong. (12) Data banks. It would appear to be worth considering establishing a bank for certain types of data. It certain items on questionnaires were to take a standard form, a greater pool of data would de available for various analysis. (13) Effectiveness measures. The meaning of a library performance measure is not readily interpreted. Each measure must itself be assessed in relation to the corresponding measures for earlier periods of time and a standard measure that may be a corresponding measure in another library, the 'norm', the 'best practice', or user expectations.

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