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An Application of $^{13}C$ Tracer for the Determination of Size Fractionated Primary Productivity in Upper Stream of Lake Shihwa ($^{13}C$ 추적자를 사용한 시화호 상류역에서의 식물플랑크톤 크기에 따른 1차생산성 측정에 관한 연구)

  • Lee, Yeon-Jung;Kim, Min-Seob;Won, Eun-Ji;Shin, Kyung-Hoon
    • Korean Journal of Ecology and Environment
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    • v.39 no.1 s.115
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    • pp.93-99
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    • 2006
  • Primary productivity was determined by using $^{13}C$ tracer according to different cell size of phytoplank-ton through in situ incubation experiments in upper stream of the Lake Shihwa. The average concentration of chlorophyll a was 14 ${\mu}g\;L^{-1}$ demonstrating an eutrophic water. The ratio of POC/Chl-a was lower than 30, reflecting that the origin of organic matter might be mainly phytoplankton. The primary productivity was 93.9 mgC m^{-2}\;d^{-1}$ at St. 1, which was about 40-fold lower than the average value of the lake (3,972 mgC m^{-2}\;d^{-1}$) determined by Choi et al. (1997) before opening of gate but it was higher than the average primary productivity (3.98 mgC m^{-2}\;d^{-1}$) reported by KOWACO in 1993 before constructing dam. The fractionated size (20 ${\sim}$ 53 ${\mu}m$) of phytoplankton community account for 51% of total primary productivity, indicating the highest assimilation rate. This study suggest that $^{13}C$ tracer methodology should be applied as a useful approach for the water ecological research in the future.

Sexual Maturation Inducement of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Environmental Condition (환경조절에 의한 돌돔 Oplegnathus fasciatus 성 성숙 유도)

  • Kim, Sung-Yeon;Bang, In-Chul;Kim, Seok-Min
    • Korean Journal of Ichthyology
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    • v.12 no.1
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    • pp.46-53
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    • 2000
  • Artificial gonadal maturation and spawning inducement of striped knife-jaw Oplegnathus fasciatus were studied. Effects of water temperature and photoperiod under different regims on gonadal activity and maturation of three years old O. fasciatus were investigated histologically. In experiment I (Exp. I), water temperature was gradually increased from $14.5^{\circ}C$ to $21.0^{\circ}C$ and photoperiod was also gradually increased from 10 : 30 L to 15 : 30 L from December 1996 to February 1997 and this conditions were maintained till April. In experiment II (Exp. II), water temperature was increased in the same way from Exp. I and photoperiod was controlled as natural condition till early March and then increased to 15 : 30 L immediately. Control fish were reared in net-cage culture system in the sea from December 1996 to April 1997. Gonadal activity was initiated by increasing water temperature in both Exp. I and II from January. In Exp. I, gonadal maturation and spawning were induced from February when water temperature and photoperiod reached at $21.0^{\circ}C$ and 15 : 30 L, respectively. In Exp. II, complete gonadal maturation was not induced until early March but after treated by compensatory long photoperiod (15 : 30 L), the gonad was matured and subsequently spawning occurred.

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A study of comparison about estimation methods of sediment yield (토사유출량 산정식에 대한 비교연구)

  • Kwon, Hyuk Jae;Kim, Hyeong Gi
    • Journal of Korea Water Resources Association
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    • v.53 no.12
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    • pp.1109-1117
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    • 2020
  • In this study, results of RUSLE which is most popular equation for estimating sediment and MSDPM and LADMP have been compared and analyzed by applying to real watershed of mountain area. Crop factor (C), preservation factor (P), and soil erosion factor (VM) of RUSLE can be subjectively selected and differently applied. Therefore, effects of those factors were estimated and compared with different values of factors. Furthermore, sediment yield has been estimated by MSDPM and LADMP according to 10, 20, 30, 50, 100, and 200 year return period. From the results, it was found that sediment yield can be resulted with 400% diffrence. And it was also found that MSDPM and LADMP can be applied in mountain area of Korea.

Biochemical Characterization of Lectin Purified from Kidney Bean Seedling (강낭콩 유식물로부터 분리한 Lectin의 생화학적 특성)

  • Roh, Kwang-Soo
    • KSBB Journal
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    • v.22 no.1
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    • pp.53-57
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    • 2007
  • We have studied biochemical characterization of lectin purified from kidney bean seedling through PBS extraction, $(NH_4)_2SO_4$ precipitation, and Sephadex G-100 affinity chromatography. The lectin was agglutinated by rabbit erythrocytes. This lectin analyzed by SDS-PAGE is a tetramer composed of two subunits with molecular weights of 46 and 44 kDa. The optimal temperature and thermal stability of the lectin was 30$^{\circ}C$ and 40-80$^{\circ}C$, respectively. The maximal pH of this lectin was pH 8.2.

Physical and Chemical Properties of Cement Mortar with Gamma-C2S

  • Lee, Sung-Hyun;Kim, Kyungnam;Mabudo, Mabudo;Song, Myong-Shin
    • Journal of the Korean Ceramic Society
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    • v.53 no.2
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    • pp.194-199
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    • 2016
  • Presently, for the cement industry, studies that seek to reduce $CO_2$, because of the development of the plastic industry and demand for reduction of energy use, have been actively conducted among them, studies attempting to use Gamma-$C_2S({\gamma}-C_2S)$ to fix $CO_2$ have been actively conducted. The ${\gamma}-C_2S$ compound has an important function in reacting to $CO_2$ and stiffening through carbonatization in the air. The ${\gamma}-C_2S$ compound, reacting to $CO_2$ in the air, generates $CaCO_2$ within the pore structure of cement materials and densifies the pore structure this leads to an improvement of the durability and to the characteristic of resistance against neutralization. Therefore, in this experiment, in order to synthesize ${\gamma}-C_2S$, limestone sludge and waste foundry sands are used these materials are plasticized for 30 or 60 minutes at $1450^{\circ}C$, and are prevented from being cooled in the temperature range of $30{\sim}1000^{\circ}C$ when they are about to be cooled. XRD analysis and XRF analysis are used to determine the effects of this process on ${\gamma}-C_2S$ synthesization, the temperature at which a thing is plasticized, and the conditions for cooling that obtain in the plasticized clinker also, in order to confirm the $CO_2$ capture function, analysis of the major hydration products is conducted through an analysis of carbonatization depth and compressive strength, and through MIP analysis and XRD Rietveld analysis. As a result of these analyses, it is found that when ${\gamma}-C_2S$ was synthesized, the clinker that was plasticized at $1450^{\circ}C$ for one hour demonstrated the highest yield rate the sample with which the ${\gamma}-C_2S$ was mixed generated $CaCO_3$ when it reacted with $CO_2$ therefore, carbonatization depth and porosity were reduced, and the compressive strength was increased.

A Biomechanical Comparison of Intralaminar C7 Screw Constructs with and without Offset Connector Used for C6-7 Cervical Spine Immobilization : A Finite Element Study

  • Qasim, Muhammad;Hong, Jae Taek;Natarajan, Raghu N.;An, Howard S.
    • Journal of Korean Neurosurgical Society
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    • v.53 no.6
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    • pp.331-336
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    • 2013
  • Objective : The offset connector can allow medial and lateral variability and facilitate intralaminar screw incorporation into the construct. The aim of this study was to compare the biomechanical characteristics of C7 intralaminar screw constructs with and without offset connector using a three dimensional finite element model of a C6-7 cervical spine segment. Methods : Finite element models representing C7 intralaminar screw constructs with and without the offset connector were developed. Range of motion (ROM) and maximum von Mises stresses in the vertebra for the two techniques were compared under pure moments in flexion, extension, lateral bending and axial rotation. Results : ROM for intralaminar screw construct with offset connector was less than the construct without the offset connector in the three principal directions. The maximum von Misses stress was observed in the C7 vertebra around the pedicle in both constructs. Maximum von Mises stress in the construct without offset connector was found to be 12-30% higher than the corresponding stresses in the construct with offset connector in the three principal directions. Conclusion : This study demonstrated that the intralaminar screw fixation with offset connector is better than the construct without offset connector in terms of biomechanical stability. Construct with the offset connector reduces the ROM of C6-7 segment more significantly compared to the construct without the offset connector and causes lower stresses around the C7 pedicle-vertebral body complex.

Usefulness of Cardiac Troponin I as a Prognostic Marker in Noncardiac Critically Ill Patients (비순환기계 중환자의 예후인자로서 Cardiac Troponin I의 유용성)

  • Kim, Hwi Jong;Ham, Hyoun Seok;Cho, Yu Ji;Kim, Ho Cheol;Lee, Jong Deok;Hwang, Young Sil
    • Tuberculosis and Respiratory Diseases
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    • v.59 no.1
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    • pp.53-61
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    • 2005
  • Background : Cardiac troponin I (cTnI) is a specific marker of myocardial injury. It is known that a higher level of cTnI is associated with a poor clinical outcome in patients with acute coronary syndrome. An elevation in cTnI is also observed in various noncardiac critical illnesses. This study evaluated whether cTnI is useful for predicting the prognosis in noncardiac critically ill patients. Methods : From June 2003 to July 2004 at Gyeongsang National University Hospital, we enrolled 215 patients (male:142, female:73, mean age:$63{\pm}15$ years ) who were admitted for critical illness other than acute coronary syndrome at the medical intensive care unit(ICU). The severity score of critical illness (SAPS II and SOFA) was determined and serum cTnI level was measured within 24 hours after admission to the ICU. The mortality rate was compared between the cTnI-positive (${\geq}0.1{\mu}g/L$) and cTnI-negative ($cTnI<0.1{\mu}g/L$) patients at the $10^{th}$ and $30^{th}$ day after admission to the ICU. The mean cTnI value was compared between the survivors and non-survivors at the $10^{th}$ and $30^{th}$ day after admission to the ICU in the cTnI-positive patients. The correlation between cTnI and the severity of the critical illness score (SAPS II and SOFA) was also analyzed in cTnI-positive patients. Results : 1) The number of cTnI-negative and positive patients were 95(44%) and 120(56%), respectively. 2) The mortality rate at the $10^{th}$ and $30^{th}$ day after admission to the ICU was significantly higher in the cTnI-positive patients (29%, 41%) than in the cTnI-negative patients (12%, 21%)(p<0.01). 3) In the cTnI-positive patients, the mean value of the cTnI at the $10^{th}$ and $30^{th}$ day after admission to the ICU was significantly higher in the non-survivors ($4.5{\pm}9.2{\mu}g/L$, $3.5{\pm}7.9{\mu}g/L$) than in the survivors($1.8{\pm}3.6{\mu}g/L$, $2.0{\pm}3.9{\mu}g/L$) (p < 0.05). 4) In the cTnI-positive patients, the cTnI level was significantly correlated with the SAPS II score (r=0.24, p<0.001) and SOFA score (r=0.30, p<0.001). Conclusion : The cTnI may be a useful prognostic marker in noncardiac critically ill patients.

Studies on the Press Drying and the Chemical Absorption of the Plywood Treated with Diammonium Phosphate (제2인산(第2燐酸)암모늄 처리합판(處理合板)의 약제흡수(藥劑吸收) 및 열판건조(熱板乾燥)에 관(關)한 연구(硏究))

  • Kim, Jong-Man
    • Journal of the Korean Wood Science and Technology
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    • v.23 no.4
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    • pp.39-45
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    • 1995
  • The plywoods commonly used as decorative interior materials for the construction are inflammable and so it is a causative factor for making fire accidents, resulting in the destruction of human life and personal properties. Indeed, it is, therefore, required to produce fire-retardant plywoods. In this study, a special grade of defect-free, Kapur plywood was used. Specimens were cut into 3- by 20cm dimensions from 120- by 240- by 0.33-cm panels(thin panel) or 120- by 240- by 0.5-cm panels(thick panel). Some specimens were treated with diammonium phosphate(DAP), but some were not treated with diammonium phosphate to use as control panels. Chemical absorption, drying curves, drying rates and dynamic Young's modulus were investigated. The results were summaries as follows; 1. The specimens were soaked into 19% diammonium phosphate solution by a full cell pressure process and the diammonium phosphate retained in the thin and thick plywoods was 1.409kg/$(30cm)^3$, 1.487kg/$(30cm)^3$, respectively. 2. Diammonium phosphate-treated plywoods were redried with press-drying process at one of either condition dried on the platen($115^{\circ}C$) for a period of time or dried on the platen($50^{\circ}C$) for 3 hrs plus in a dry-oven($30^{\circ}C$) for 24 hrs. or dried on the platen($60^{\circ}C$) for 2 hrs plus in a dry-oven($30^{\circ}C$) for 24 hrs. The drying rate of treated thin specimens dried at $60^{\circ}C$ plus $30^{\circ}C$ and $115^{\circ}C$ only was found to be 0.04 %/min. and 8.53 %/min. Similarly, the drying rate of treated thick specimens were 0.03 %/min. and 6.77 %/min. respectively. 3. It was evident that highly-significantly different drying rate of treated plywoods was observed between plywood thicknesses and platen temperatures and the rate was increased by elevating the platen temperature up to $115^{\circ}C$. Based on the two-way variance analysis, highly significant drying rate was observed from the interaction between plywood thicknesses and platen temperatures. 4. After redrying, the specimens were weighed and reconditioned to a constant weight in a facility maintained temperature ($20^{\circ}C$) and relative humidity(65%) prior to test dynamic Young's modulus. The test revealed that the thin specimens dried at the platen temperature of $50^{\circ}C$, $60^{\circ}C$, $115^{\circ}C$ and untreated specimens showed 1.070E+09 dyne/$cm^2$, 1.156E+09 dyne/$cm^2$, 1.243E+09 dyne/$cm^2$, and 1.052E+09 dyne/$cm^2$, respectively. Likewise, the thick specimens revealed 5.647E+09 dyne/$cm^2$ 5.670E+09 dyne/$cm^2$, 6.395E+09 dyne/$cm^2$ and 5.415E+09 dyne/$cm^2$, respectively. 5. It was evident that significantly different dynamic Young's modulus was observed between the plywood thickness and the platen temperature, but not in the two-way interaction between the plywood thickness${\times}$the platen temperature.

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Expression of Jun and p53 Genes from the Brain of Rats Irradiated with $^{60}Co{\gamma}$-ray (감마선 조사에 의한 뇌조직의 Jun 및 p53유전자 발현)

  • Kim Yong Seok;Woo Chong Kyu;Lee Yong Sung;Koh Jai Kyung;Chun Ha Chung;Lee Myung Za
    • Radiation Oncology Journal
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    • v.14 no.4
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    • pp.265-279
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    • 1996
  • Damage produced by radiation elicits a complex response in mammalian cells, including growth rate changes and the induction of a variety of genes associated with growth control and apoptosis. At doses of 10,000 cGy or greater, the exposed individual was killed in a matter of minutes to a couple of days, with symptoms consistent with pathology of the central nervous system(CNS) including degenerative changes. The nature of the damage in irradiated cells underlies the unique hazards of ionizing radiation. Radiation injury to CNS is a rare event in clinical medicine, but it is catastrophic for the patient in whom it occurs. The incidence of cerebral necrosis has been reported as high as 16% for doses greater than 6,000 cGy. In this study, the effect of radiation on brain tissue was studied in vivo. Jun and p53 genes in the rat brain were induced by whole body irradiation of rat with 600Co in doses between 1 Gy and 100 Gy and analyzed for expression of jun and p53 genes at the postirradiation time up to 6 hours. Northern analyses were done using 1.8 Kb & 0.8 Kb-pGEM-2-JUN/Eco RI/Pst I fragments, 2.0 Kb-php53B/Bam HI fragment and ,1.1 Kb-pBluescript SK--ACTIN/Eco RI fragment as the digoxigenin or [${\alpha}^{32}P$] dCTPlabeled probes for Jun, p53 and ${\beta}$-actin genes, respectively. Jun gene seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 1 hour after irradiation of $^{60}$Co in dose of 30 Gy. Jun was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in doses of 1 Gy and 10 Gy. After irradiation of $^{60}$Co in dose between 20 Gr and 100 Gy, the expression of Jun was however increased to peak in 2 hours and decreased thereafter. p53 gene in this study also seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 6 hours after irradiation of $^{60}$Co in dose of 1 Gy, p53 was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in dose between 1 Gy and 40 Gy. After irradiation of $^{60}$Co in doses of 50 Gy and 100 Gy, the expression of p53 was however increased to peak in 2 hours and decreased thereafter. The expression of Jun and p53 genes was not correlative in the brain tissue from rats. It seemed to be very important for the establishment of the optimum conditions for the animal studies relevant to the responses of genes inducible on DNA damage to ionizing radiation in mammalian cells. But there are many limitations to the animal studies such as the ununiform patterns of gene expression from the tissue because of its complex compositions. It is necessary to overcome the limitations for development of in situ Northern analysis.

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Treeing Phenomena of Epoxy-Layered Silicate Nanocomposites (Epoxy-Layered Silicate Nanocomposites Treeing 현상에 관한 연구)

  • Park, Jae-Jun;Cho, Dae-Ryng;Cho, Hog-Sok;Kim, Kyung-Tae;Hwang, Byung-Jun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.371-372
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    • 2008
  • 본 논문은 새롭게 초음파 분산기법을 이용하여 제조된 나노콤포지트 와 원형에폭시 수지에 대한 전기적 특성인 트리현상의 여러특성을 연구하였다. 나노필러인 Layered Silicate Particles가 에폭시수지 중에 Power Ultrasonic으로 분산된 나노콤포지트를 제조하였다. 충진된 혼합물에서 나노입자의 영향을 조사하기위해 열적, 구조적 특성을 연구하였고, 장시간 절연파괴 특성을 조사하기위해 침대평판 전극으로 원형에폭시수지와 나노콤포지트와 비교 측정하였다. 연구는 에폭시원형수지에 대한 인가전압레벌(교류 10, 15, 20kV)의 변화와 온도변화에 대한 (30,90,$130^{\circ}C$)의 트리특성을 연구하였다. 모든 전압레벨에서는 일정전압까지 1kV/s 로 승압 후 일정하게 인가되었고, 파괴에 이를 때까지 측정한 결과 10kV, 15Kv, 20KV의 경우 1042,75,488분후에 파괴에 이르렀다. 그러나 트리진행속도는 인가전압이 높을수록 빠르게 진행하였다. 온도 변화에 대한 트리특성으로서 15kV인가 후 파괴에 이르는 시간은 30,90,$130^{\circ}C$의 경우 75.3, 970, 226분으로 $90^{\circ}C$의 경우 절연성능이 가장 우수하였고, 트리진전속도는 $30^{\circ}C,130^{\circ}C,90^{\circ}C$ 순으로 나타났다. 이는 트리진전으로 파괴에 이르는 시간과 속도는 트리형태에 지배적으로 영향을 맡고 있음을 알 수 있었다. 또한 나노콤포지트 트리의 경우 15kV인가시 10902에 파괴에 이르렀고, 트리진전속도는 0.000729mm/min으로 원형에 비하여 53.36배의 트리진전시간이 느리고, 파괴시간은 145배 오래 견디는 절연내력을 측정할 수 있었다.

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