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Biochemical Characterization of Lectin Purified from Kidney Bean Seedling  

Roh, Kwang-Soo (Department of Biology, Keimyung University)
Publication Information
KSBB Journal / v.22, no.1, 2007 , pp. 53-57 More about this Journal
Abstract
We have studied biochemical characterization of lectin purified from kidney bean seedling through PBS extraction, $(NH_4)_2SO_4$ precipitation, and Sephadex G-100 affinity chromatography. The lectin was agglutinated by rabbit erythrocytes. This lectin analyzed by SDS-PAGE is a tetramer composed of two subunits with molecular weights of 46 and 44 kDa. The optimal temperature and thermal stability of the lectin was 30$^{\circ}C$ and 40-80$^{\circ}C$, respectively. The maximal pH of this lectin was pH 8.2.
Keywords
Hemagglutination activity; kidney bean seedling; lectin;
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Times Cited By KSCI : 4  (Citation Analysis)
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