• Journal of Oral Medicine and Pain
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• 제32권1호
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• pp.45-55
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• 2007

천연 식물 추출물을 구강 질환 분야에 활용하는 방안이 다양하게 모색되고 있다. 본 연구는 편백나무에서 추출한 휘발성 정유인 피톤치드를 치의학분야에 활용하고자 치아우식증 원인균인 Streptococcus mutans GS5와 Streptococcus sobrinus 6715, 급진성 치주염에 관련된 Actinobacillus actinomycetemcomitans Y4에 대한 항균효과를 미생물학적으로 실험하였다. 흡광도 측정, 생균수 검사, 항생제 감수성 검사를 통해 다음과 같은 결과를 얻을 수 있었다. 1. 피톤치드의 최소억제농도(minimum inhibitory concentration; MIC)는 S. mutans GS5는 0.5%, S. sobrinus 6715는 1%, A. actinomycetemcomitans Y4는 0.2%로 측정되었다. 2. 피톤치드의 최소살균농도(minimum bactericidal concentration; MBC)는 S. mutans GS5는 0.5%, S. sobrinus 6715는 2%, A. actinomycetemcomitans Y4는 0.2%로 측정되었다. 3. 피톤치드에 노출된 실험균주에 대한 항생제 감수성 실험에서 피톤치드를 적용했을 경우, S. mutans GS5과 S. sobrinus 6715는 ampicillin에 대한 감수성이 유의성 있게 증가하였다. S. sobrinus 6715의 경우는 penicillin과 amoxicillin에 대한 감수성도 피톤치드에 의해 유의성 있게 증가하였다. 반면, A. actinomycetemcomitans Y4는 amoxicillin과 cefotaxime에 대한 감수성이 다소 증가하였으나 유의성은 없었다. 이상의 결과로, 편백 피톤치드 정유는 치아우식증 원인균인 Streptococcus mutans와 Streptococcus sobrinus, 급진성 치주염 원인균인 Actinobacillus actinomycetemcomitans에 대한 살균효과가 있을 뿐만 아니라 이들 균의 항생제 감수성을 높이는 것으로 판단된다. 따라서, 피톤치드는 치아우식증과 치주질환을 포함한 구강질환에 대해 예방적이고 치료적인 효과를 얻을 가능성이 있는 것으로 생각된다.

• 약학회지
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• 제54권1호
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• pp.22-26
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• 2010

We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, $\varepsilon$-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration ($IC_{50}$) of compound 2 against S. aureus was 7.2 ${\mu}M$. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 ${\mu}M$, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.

• 약학회지
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• 제44권4호
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• pp.318-324
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• 2000

9 New cephalosporin derivatives were synthesized and screened for antibacterial activities against various bacteria. (6R,7R) -7$\beta$-[(Z)-2-(5-amino-1,2,4-thiadiazol-3-yl)-2-(fluoromethoxyimino)acetamido]-3-[(2-hydroxycarbamoyl)-s-triazolo[1,5-a]-pyrimidin-7-yl]thiomethyl]-3-cephem-4-carboxylic acid sodium salt (compound 3) showed 2 fold enhanced in vitro activity against P. aerurginosa, compared to ceftazidime.

• Journal of the Korean Wood Science and Technology
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• 제51권4호
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• pp.283-294
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• 2023

Chemical characterization of vinegars obtained from Durian wood (Durio sp.) and their termicidal activity against Coptotermes curvignathus and antifungal activity against Schizophyllum commune were evaluated. The process of pyrolysis produced wood vinegars at three distinct temperature: 350℃, 400℃, and 450℃. To determine their effectiveness against fungal growth, the vinegars were tested using a Petri dish with 1.0%, 2.0%, 3.0%, and 4.0% (v/v) against S. commune. In the experiment, termicidal activities were evaluated using a no-choice test for C. curvignathus with 3.0%, 6.0%, 9.0%, and 12.0% (v/v). The wood vinegar exhibited antitermitic activity to C. curvignathus workers in the no-choice experiment; For vinegar produced at 450℃, a 6% concentration was required to achieve 100% mortality against C. curvignathus. In addition, a 12% vinegar produced at 450℃ resulted in the lowest mass loss of treated filter paper, which was 20.00%. Furthermore, all the wood vinegars exhibited antifungal activities against S. commune at concentration of 2.0%. The dominant chemical components of wood vinegar produced at temperature of 350℃, 400℃, and 450℃ were 2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 3.5-dimethoxy-4-hydroxytoluene, and creosol.

• 농약과학회지
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• 제15권1호
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• pp.1-7
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• 2011

벼의 줄기에 주로 발생하는 잎집무늬마름병에 대한 thifluzamide의 방제 효과를 증진하기 위하여 벼 경엽을 통한 thifluzamide의 침투성을 강화하거나 벼 부착량을 증진할 수 있는 계면활성제를 선발하고 이를 함유하는 제제의 병 방제 효과를 측정하였다. 수화제 현탁액에 dodecaethylene glycol monohexadecyl ether를 첨가함으로써 벼 잎에 대한 thifluzamide의 침투성을 증진할 수 있었으나 병 예방효과는 오히려 감소하였다. 비이온성 계면활성제와 음이온성 계면활성제를 혼합하여 수화제 현탁액에 첨가하였을 때 유효성분 부착량이 약 5배 증대되었으나 잎집무늬마름병 예방효과는 약간 증가하여 $EC_{50}$가 5.8 mg $L^{-1}$에서 각각 4.4 mg $L^{-1}$와 3.4 mg $L^{-1}$로 감소하였다.

• 약학회지
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• 제34권2호
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• pp.117-121
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• 1990

$7{\beta}$-[4-Phenyl-5-(3.4.5-trimethoxyphenyl)-4H-1.2.4-triazol-3-yl]thioacetoamidocepharosporanic acid was synthesized by condensation of 7-Aminocephalosporanic acid (7-ACA) with [4-Phenyl-5-(3.4.5-trimethoxyphenyl)-4H-1.2.4-triazol-3-yl] thioacetylchloride. This compound was tested for antimicrobial activity in vitro against nine species of microorganisms. It showed remakable antibacterial activity against Bacillus subtilis, Micrococcus luteus, and Staphylococcus aureus.

• Biomolecules & Therapeutics
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• 제20권5호
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• pp.477-481
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• 2012

The rhizomes of Acorus gramineus have frequently been used in traditional medicine mainly for sedation as well as enhancing brain function. In this study, the anti-allergic activity of A. gramineus was investigated. The 70% ethanol extract of the rhizomes of A. gramineus was found to inhibit the allergic response against 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat basophilic leukemia (RBL)-1 cells and ${\beta}$-hexosaminidase release from RBL-2H3 cells with $IC_{50}$'s of 48.9 and > $200{\mu}g/ml$, respectively. Among the 9 major constituents isolated, ${\beta}$-asarone, (2R,3R,4S,5S)-2,4-dimethyl-1,3-bis (2',4',5'-trimethoxyphenyl)tetrahydrofuran (AF) and 2,3-dihydro-4,5,7-trimethoxy-1-ethyl-2-methyl-3-(2,4,5-trimethoxyphenyl)indene (AI) strongly inhibited 5-LOX-catalyzed LT production in A23187-treated RBL-1 cells, AI being the most potent ($IC_{50}=6.7{\mu}M$). Against ${\beta}$-hexosaminidase release by antigen-stimulated RBL-2H3 cells, only AI exhibited strong inhibition ($IC_{50}=7.3{\mu}M$) while ${\beta}$-asarone and AF showed 26.0% and 39.9% inhibition at $50{\mu}M$, respectively. In addition, the ethanol extract of A. gramineus showed significant inhibitory action against the hapten-induced delayed hypersensitivity reaction in mice by oral administration at 200 mg/kg. Therefore, it is suggested that A. gramineus possesses anti-allergic activity and the constituents including ${\beta}$-asarone and AI certainly contribute to the anti-allergic activity of the rhizomes of A. gramineus.

• Nuclear Engineering and Technology
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• 제5권2호
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• pp.83-86
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• 1973

Acetylbenzoylaconine, 2-aminoethylisothiouronium bromide hydrobromide (AET), $\beta$-mercaptoethylamine HCI (MEA), L-thiazolidine-4-carboxylic acid (thioproline)의 Co-60 감마선에 대한 방어효과를 5-6주된 백색마우스를 이용하여 연구하였다. 백색마우스에 대한 acetylbenzoylaconine의 독성실험 결과 LD$_{50}$은 2.5mg/kg이었다. 위의 4종 실험약제를 백색마우스 복강내에 주사한 후 Co-60 감마선 800 rad를 전신조사하고 30일간 계속 관찰하여 실험군들에 대한 생존계수를 계산하였다. 이 생존계수치로 방사선장해에 대한 방어계수를 계산한 결과 acetylbenzoylaconine AET, MEA, thioproline에 대해 각각 2.24, 2.95, 2.78, 1.23 으로, acetylbenzoylaconine은 백색마우스를 Co-60 감마선장해로 부터 방어할 수 있는 능력이 있음을 알았다.

• Bulletin of the Korean Chemical Society
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• 제31권10호
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• pp.2854-2860
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• 2010

Synthesis of a new series of pyrimidinyl-imidazo[2,1-b]thiazole derivatives is described. Their antiproliferative activity against A375 human melanoma cell line was tested and the effect of substituents on the pyrimidinyl ring side chain was investigated. The biological results indicated that most of the newly synthesized compounds showed moderate activity against A375, compared with Sorafenib. Among all of these derivatives, the cyclic sulfamide derivatives IIIa, IIIb, and IIIe showed the most potent antiproliferative activity against A375 human melanoma cell line. The IC50 values of compounds IIIa,b were in nanomolar scale. In addition, compound IIIe ($IC_{50}=1.9\;{\mu}M$) also demonstrated more potent antiproliferative activity compared with Sorafenib ($IC_{50}=5.6\;{\mu}M$).

• Steel and Composite Structures
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• 제46권2호
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• pp.279-292
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• 2023

Structural design against the progressive collapse has been a vital necessity for decades due to occasional tragic events. The question of whether designed structures against the progressive collapse are still robust if subjected to multi-hazard scenarios containing column removal and successive localized fires is ad-dressed in the current study. Two seven-story steel structures with an identical area but different structural configurations of 4- and 5-bays are designed against the progressive collapse; the structural components are also fireproofed for a 60 min fire resistance. The structures are then subjected to different column re-moval scenarios over different stories followed immediately by localized fires. Results indicate that the structures are not able to keep their stability under all of the considered scenarios; the 4-bay structure is more vulnerable than the 5-bay structure. It is also indicated that upper stories are more sensitive toward the considered scenarios than lower stories. To advance structural safety, two strategies are adopted: in-creasing the thickness of the insulation materials to reduce the thermal effects, or, increasing the safety fac-tor (ΩN) of the structures when designing against the progressive collapse. As for the first strategy, provid-ing a 35% and a 25% increase in the insulation thicknesses of the structural components of the 4-bay and 5-bay structures, respectively, can prevent a progressive collapse to trigger. As for the second strategy, in-creasing ΩN by 10% can enhance the structural integrity to where no collapse occurs under all of the sce-narios.